FastQCFastQC Report
Fri 17 Jun 2016
SRR1527763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585786
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT19970.3409094788881947No Hit
TTGGACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA17150.2927690316941682No Hit
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG16820.28713557510763316No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC16560.2826970941606662No Hit
TTGGACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG12690.2166320123731192No Hit
TTGGACGGGCTGGGGCTACAGAGCTGACTGACGTCACAGGATTCAAACCAAT8670.14800626850078358No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT8560.14612844963860522No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT8420.14373849835946914No Hit
TTGGACGGGAGCGTAGAGCCGGGCGCGGTGGCGCGCGTCTTTAATCCCAGCA7920.13520295807684035No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC6780.11574192623244667No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGC6700.11437623978722605No Hit
TTGGACGGGGATATTACCTGTGAGAAACTCACTTTAAATACAATGGTGTGTG6660.11369339656461576No Hit
TTGGACGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT6540.11164486689678482No Hit
TTGGACGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA6370.10874278320069104No Hit
TTGGACGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCA6160.10515785628198693No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT6010.10259719419719829No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC6000.10242648339154571No Hit
TTGGACGGGGGGAGCATGTAGAAGGGGAGGGAGGGAGAGAGGGAGGGAAGAA5940.10140221855763026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGC301.8598294E-646.046
TTAGGCG206.308907E-446.020
TAATAGT253.4150893E-546.035
TAATAGA253.4150893E-546.016
GGTACGA253.4150893E-546.015
GCCTTAA206.308907E-446.025
TCGCAAT206.308907E-446.043
CGTATTG253.4150893E-546.020
CGTATTA301.8598294E-646.040
GTCGAAT301.8598294E-646.022
ATAGGCG405.6024874E-946.010
CGACGTC206.308907E-446.036
CCGATTA501.6370905E-1146.037
CACGGGT206.308907E-446.017
ACGCATA551.8189894E-1246.033
CCGATGA301.8598294E-646.046
TCCGATT600.046.036
TCCGATA253.4150893E-546.021
CCGATAG253.4150893E-546.022
GCGCGAT253.4150893E-546.033
Produced by FastQC (version 0.11.5)