##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527763_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	585786
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22445910281229	33.0	31.0	34.0	31.0	34.0
2	32.772389575715366	34.0	31.0	34.0	31.0	34.0
3	32.629294657093205	34.0	31.0	34.0	31.0	34.0
4	36.30027860003482	37.0	37.0	37.0	35.0	37.0
5	35.82155770195942	37.0	35.0	37.0	35.0	37.0
6	36.17558801337007	37.0	37.0	37.0	35.0	37.0
7	36.396728498120474	37.0	37.0	37.0	35.0	37.0
8	36.57762561754634	37.0	37.0	37.0	35.0	37.0
9	38.61811822064713	39.0	39.0	39.0	38.0	39.0
10	37.88373057737809	39.0	38.0	39.0	35.0	39.0
11	37.60665499004756	39.0	37.0	39.0	35.0	39.0
12	37.227214375215524	39.0	37.0	39.0	34.0	39.0
13	36.954025872929705	39.0	37.0	39.0	33.0	39.0
14	37.93913306224457	40.0	38.0	40.0	33.0	40.0
15	38.025729532627956	40.0	38.0	40.0	34.0	40.0
16	38.063113491957814	40.0	38.0	40.0	34.0	40.0
17	38.027960722857834	40.0	38.0	40.0	34.0	40.0
18	38.03269965482275	40.0	38.0	40.0	34.0	40.0
19	37.97411170632279	40.0	38.0	40.0	34.0	40.0
20	37.90907259647721	40.0	38.0	40.0	34.0	40.0
21	37.929776402986754	40.0	38.0	40.0	34.0	40.0
22	37.77643542180933	40.0	37.0	40.0	33.0	40.0
23	37.72939605931176	40.0	37.0	40.0	33.0	40.0
24	37.71327413082593	40.0	37.0	40.0	33.0	40.0
25	37.68027743920135	40.0	37.0	40.0	33.0	40.0
26	37.47325473807841	40.0	37.0	40.0	33.0	40.0
27	37.41481189376325	40.0	36.0	40.0	33.0	40.0
28	37.26239957936858	39.0	36.0	40.0	33.0	40.0
29	37.1581208837357	39.0	36.0	40.0	32.0	40.0
30	37.11738757839894	39.0	36.0	40.0	32.0	40.0
31	37.0208233040735	39.0	35.0	40.0	32.0	40.0
32	36.90623538288727	39.0	35.0	40.0	32.0	40.0
33	36.60504860136637	39.0	35.0	40.0	31.0	40.0
34	36.595179809691594	39.0	35.0	40.0	31.0	40.0
35	36.610588167009794	39.0	35.0	40.0	31.0	40.0
36	36.378095413683496	38.0	35.0	40.0	31.0	40.0
37	36.34053903643993	38.0	35.0	40.0	31.0	40.0
38	35.96732424469004	38.0	35.0	40.0	30.0	40.0
39	36.0483691996736	38.0	35.0	40.0	30.0	40.0
40	35.960200482770155	38.0	35.0	40.0	30.0	40.0
41	35.63828258101081	38.0	34.0	40.0	29.0	40.0
42	35.653539005711984	38.0	34.0	40.0	29.0	40.0
43	35.58756098643532	38.0	34.0	40.0	29.0	40.0
44	35.49228216447645	37.0	34.0	40.0	29.0	40.0
45	35.36642220879297	37.0	34.0	40.0	29.0	40.0
46	35.00783904019556	37.0	34.0	40.0	28.0	40.0
47	34.7474299488209	36.0	33.0	40.0	27.0	40.0
48	34.76878928482415	36.0	33.0	39.0	28.0	40.0
49	34.6581208837357	36.0	33.0	39.0	27.0	40.0
50	34.56158050892305	36.0	33.0	39.0	27.0	40.0
51	34.92122037740745	36.0	34.0	39.0	28.0	40.0
52	34.60463548121669	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	12.0
17	42.0
18	72.0
19	144.0
20	309.0
21	496.0
22	738.0
23	1163.0
24	1765.0
25	2462.0
26	3421.0
27	4585.0
28	6284.0
29	7885.0
30	10102.0
31	12921.0
32	16997.0
33	23754.0
34	38901.0
35	48130.0
36	69739.0
37	103725.0
38	179220.0
39	52916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.03753247773078	0.8508226553724397	0.08501398121498295	0.026630885681801886
8	99.41275482855514	0.4597241996223877	0.11113273447982709	0.016388237342647317
9	96.89340475873442	1.7209356317836206	0.5725640421587406	0.8130955673232205
10	47.392733865268205	39.01151615094932	5.170488881605228	8.425261102177245
11	32.500435312554416	24.418302929738847	23.607085181277803	19.474176576428935
12	27.38576886439758	19.62354853137494	27.261832819493808	25.728849784733676
13	24.31843710843209	19.287248244239365	30.42732328870953	25.96699135861902
14	25.217058789387252	19.727340701211705	30.50892305381146	24.54667745558958
15	25.037641732646392	22.503098401122596	27.6887805444309	24.770479321800114
16	28.380842150546442	23.471711512395313	24.587135916529245	23.560310420529
17	28.83681071244448	23.126875684977108	23.15794505160588	24.87836855097254
18	27.428958698227678	22.859713274130826	24.271662347683286	25.43966567995821
19	28.37606224798817	23.512340684140625	23.28068612087008	24.830910947001122
20	28.391426220496907	22.908707275353116	24.21720560068011	24.48266090346987
21	29.467587139330746	20.984284363231623	25.671149532423104	23.87697896501453
22	27.914972361920565	21.729607740710772	25.619594869116025	24.735825028252638
23	26.78964673105878	23.492538230684925	25.243177542652095	24.474637495604195
24	26.442933084778403	23.454811142635705	25.143311721345334	24.958944051240557
25	26.681586791080704	24.887245512866475	23.290416636792273	25.140751059260545
26	25.583574889123334	26.39581690241829	21.63742390565838	26.3831843028
27	26.147432680193788	23.786843659629966	23.92426585818029	26.141457801995948
28	26.380111508298253	25.069223231692117	23.21188966619209	25.338775593817537
29	26.861345269432864	23.999207901861773	24.39747621144923	24.741970617256133
30	27.63978654320861	21.916536072900342	24.856858989460314	25.586818394430733
31	27.961405701058066	24.577063978995742	24.116827646956395	23.344702672989794
32	28.75418668250863	24.492220708586412	23.36040806710983	23.393184541795126
33	29.430372183698484	23.24654395973956	23.084197983563964	24.23888587299799
34	26.995523962675787	25.94326255663331	24.13680081121775	22.924412669473153
35	27.150187952597022	25.39408589484897	24.944945765177042	22.51078038737696
36	26.85127333189936	25.46868651691915	24.008084863755705	23.67195528742578
37	26.68090394785809	26.82464244621756	23.09478205351442	23.399671552409927
38	26.933214518612598	26.39598761322394	23.56440747986466	23.1063903882988
39	26.12899591318331	24.854298327375528	22.930216836865338	26.086488922575825
40	26.24081149088575	25.50573076174576	22.77589426855541	25.47756347881308
41	25.293366519513956	26.079831201155372	23.86827271392625	24.75852956540443
42	25.22747214853206	25.822740727842593	23.248080356990435	25.701706766634913
43	25.223887221613357	24.821692563495883	25.267247766249106	24.687172448641654
44	25.294902916764826	24.965772483466658	24.369479639322208	25.369844960446308
45	25.195549227875023	24.92685041977787	24.20986503603705	25.667735316310054
46	26.084098971296687	24.146019194722985	24.175040031683924	25.594841802296404
47	24.85737112187727	24.680685438026856	25.197768468348507	25.264174971747362
48	26.606303325787916	23.980088291628686	24.65644450362419	24.75716387895921
49	24.240934402665822	25.746262286910238	25.217741632609865	24.795061677814083
50	24.38399005780268	25.66022404086134	25.118900076137017	24.836885825198962
51	25.914071008866717	24.408401703011	24.17589358571219	25.501633702410093
52	24.249811364559754	26.196085259804775	26.029300802682208	23.524802572953263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	4.0
6	8.0
7	4.5
8	1.0
9	2.0
10	3.0
11	148.5
12	294.0
13	755.5
14	1562.0
15	1907.0
16	1748.0
17	1589.0
18	1531.5
19	1474.0
20	1544.5
21	1615.0
22	1520.5
23	1426.0
24	1756.5
25	2087.0
26	2423.0
27	2759.0
28	3261.5
29	3764.0
30	4403.5
31	5043.0
32	5190.5
33	5338.0
34	6815.0
35	8292.0
36	9622.0
37	10952.0
38	12612.0
39	15707.0
40	17142.0
41	18450.0
42	19758.0
43	20806.0
44	21854.0
45	23610.5
46	25367.0
47	28090.5
48	30814.0
49	30750.0
50	30686.0
51	32192.5
52	33699.0
53	35863.5
54	38028.0
55	38574.5
56	39121.0
57	40509.5
58	41898.0
59	44353.0
60	46808.0
61	45671.5
62	44535.0
63	41798.5
64	34312.0
65	29562.0
66	24855.0
67	20148.0
68	16563.0
69	12978.0
70	12000.0
71	11022.0
72	10401.5
73	9781.0
74	8108.5
75	6436.0
76	4926.0
77	3416.0
78	2165.5
79	915.0
80	719.5
81	524.0
82	313.0
83	102.0
84	92.0
85	82.0
86	44.0
87	6.0
88	3.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	585786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.94753715520685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1923500078586	8.864158583510019
2	9.89326589223713	2.3640032366768753
3	3.5406575506879845	1.2690641292212514
4	1.6417334647863175	0.7845868627792402
5	0.8687327646562933	0.5189608491838317
6	0.5129524054467258	0.3677110753756491
7	0.3057710717704717	0.2557247868675591
8	0.22861388543586666	0.21850983123529755
9	0.13716833126152	0.14749413608382583
>10	3.37777015731493	11.571290539548572
>50	3.194879048966236	27.673757993533478
>100	2.0603826424907483	41.7005527615887
>500	0.0385785931673025	2.84404202217192
>1k	0.007144183919870833	1.4201431922237815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1997	0.3409094788881947	No Hit
TTGGACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1715	0.2927690316941682	No Hit
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1682	0.28713557510763316	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1656	0.2826970941606662	No Hit
TTGGACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	1269	0.2166320123731192	No Hit
TTGGACGGGCTGGGGCTACAGAGCTGACTGACGTCACAGGATTCAAACCAAT	867	0.14800626850078358	No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	856	0.14612844963860522	No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	842	0.14373849835946914	No Hit
TTGGACGGGAGCGTAGAGCCGGGCGCGGTGGCGCGCGTCTTTAATCCCAGCA	792	0.13520295807684035	No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	678	0.11574192623244667	No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGC	670	0.11437623978722605	No Hit
TTGGACGGGGATATTACCTGTGAGAAACTCACTTTAAATACAATGGTGTGTG	666	0.11369339656461576	No Hit
TTGGACGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	654	0.11164486689678482	No Hit
TTGGACGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA	637	0.10874278320069104	No Hit
TTGGACGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCA	616	0.10515785628198693	No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	601	0.10259719419719829	No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	600	0.10242648339154571	No Hit
TTGGACGGGGGGAGCATGTAGAAGGGGAGGGAGGGAGAGAGGGAGGGAAGAA	594	0.10140221855763026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.047457603971416185	0.0	0.0	0.0	0.0
8	0.04882329041663679	0.0	0.0	0.0	0.0
9	0.0495061336392471	0.0	0.0	0.0	0.0
10	0.05872451714448621	0.0	0.0	0.0	0.0
11	0.06657721420450471	0.0	0.0	0.0	0.0
12	0.07630773012670156	0.0	0.0	0.0	0.0
13	0.08518469202063553	0.0	0.0	0.0	0.0
14	0.09286667827500145	0.0	0.0	0.0	0.0
15	0.10157292936328284	0.0	0.0	0.0	0.0
16	0.11488837220418378	0.0	0.0	0.0	0.0
17	0.12752097182247443	0.0	0.0	0.0	0.0
18	0.13708077693901868	0.0	0.0	0.0	0.0
19	0.1519326170307928	0.0	0.0	0.0	0.0
20	0.16302881939821026	0.0	0.0	0.0	0.0
21	0.1722472029034494	0.0	0.0	0.0	0.0
22	0.18197771882564623	0.0	0.0	0.0	0.0
23	0.1929032103874111	0.0	0.0	0.0	0.0
24	0.20348728033787084	0.0	0.0	0.0	0.0
25	0.21304708545441509	0.0	0.0	0.0	0.0
26	0.22397257701617998	0.0	0.0	0.0	0.0
27	0.23780015227403864	0.0	0.0	0.0	0.0
28	0.24906706544710866	0.0	0.0	0.0	0.0
29	0.2618703758710519	0.0	0.0	0.0	0.0
30	0.27074733776498583	0.0	0.0	0.0	0.0
31	0.27928287804761465	0.0	0.0	0.0	0.0
32	0.29072050202633726	0.0	0.0	0.0	0.0
33	0.2989146206976609	0.0	0.0	0.0	0.0
34	0.31018153387073094	0.0	0.0	0.0	0.0
35	0.3233262659059793	0.0	0.0	0.0	0.0
36	0.33732455196949057	0.0	0.0	0.0	0.0
37	0.3487621759482132	0.0	0.0	0.0	0.0
38	0.3612240647608512	0.0	0.0	0.0	0.0
39	0.37300311035087896	0.0	0.0	0.0	0.0
40	0.38614784238612737	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGC	30	1.8598294E-6	46.0	46
TTAGGCG	20	6.308907E-4	46.0	20
TAATAGT	25	3.4150893E-5	46.0	35
TAATAGA	25	3.4150893E-5	46.0	16
GGTACGA	25	3.4150893E-5	46.0	15
GCCTTAA	20	6.308907E-4	46.0	25
TCGCAAT	20	6.308907E-4	46.0	43
CGTATTG	25	3.4150893E-5	46.0	20
CGTATTA	30	1.8598294E-6	46.0	40
GTCGAAT	30	1.8598294E-6	46.0	22
ATAGGCG	40	5.6024874E-9	46.0	10
CGACGTC	20	6.308907E-4	46.0	36
CCGATTA	50	1.6370905E-11	46.0	37
CACGGGT	20	6.308907E-4	46.0	17
ACGCATA	55	1.8189894E-12	46.0	33
CCGATGA	30	1.8598294E-6	46.0	46
TCCGATT	60	0.0	46.0	36
TCCGATA	25	3.4150893E-5	46.0	21
CCGATAG	25	3.4150893E-5	46.0	22
GCGCGAT	25	3.4150893E-5	46.0	33
>>END_MODULE