FastQCFastQC Report
Fri 17 Jun 2016
SRR1527762_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527762_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130351
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGA40403.0993241325344645No Hit
TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAA15661.201371681076478No Hit
TCACTTGGGGAAAGGAAAGAAAGGAAAGAAAGGAAAGAAAGGAAAGAAAG8690.6666615522704084No Hit
TCACTTGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATA7760.595315724467016No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA7050.5408474043160391No Hit
TCACTTGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTG6260.4802418086550928No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5740.44034951784029275No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4930.3782096033018542No Hit
TCACTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA4730.36286641452693114No Hit
TCACTTGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT4530.34752322575200806No Hit
TCACTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG4510.3459889068745157No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4350.33371435585457726No Hit
TCACTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.2853833112135695No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT3550.2723416007548849No Hit
TCACTTGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCT3490.267738644122408No Hit
TCACTTGGGGAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGT3370.2585327308574541No Hit
TCACTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC3340.25623125254121565No Hit
TCACTTGGGGTGCAGTGCTTCACTTGGGATTACTGTAGCTCTCTATAGTG3180.24395670152127716No Hit
TCACTTGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACT3160.24242238264378485No Hit
TCACTTGGGGAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3110.23858658545005407No Hit
TCACTTGGGGGGGAAATCGACTGGGAAGTGCCGAGGATCGCCATCTGTTT2990.22938067218510025No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2900.22247623723638485No Hit
TCACTTGGGGTTCGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTC2760.2117360050939387No Hit
TCACTTGGCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA2730.2094345267777002No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA2660.20406441070647713No Hit
TCACTTGGGGACTAAGCCCAGTATGAGAGGCGAGTGTGTGTGTGTGTGTG2620.2009957729514925No Hit
TCACTTGGGATTCAGGCCGGTGCCTACTATGGAATCAAGCCTCTGCCACC2520.19332417856403095No Hit
TCACTTGGGCTGGGCTGTGATCCTCTAGCCATCCCGACCTCACACTTCAC2510.1925570191252848No Hit
TCACTTGGGCACCACACCACCACCCCTATCTTCTCCCCCCACCCCATACT2510.1925570191252848No Hit
TCACTTGGGGGGACGCGGAGCGAGAAGCTGAGAGAGTCGGAGACGCTATC2330.17874814922785404No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGG2320.17798098978910787No Hit
TCACTTGGGGAGGAAAACGATCCAGACTATGACCCAAAGAAGGATCAGAA2280.17491235203412325No Hit
TCACTTGGGAGGTTATTTCTTTGCTTTGCGTTCCCTGGCTCCACCCCCCA2240.17184371427913864No Hit
TCACTTGGGACCTGTCTCTGGGCTTTCACTTGGGCAGAGGGAGCCATTTG2150.16493927933042324No Hit
TCACTTGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAA2110.16187064157543862No Hit
TCACTTGGGGTGCTTCACTTGGGATGGGCCATATTACTAGGCTTCAGGCA2100.16110348213669246No Hit
TCACTTGGGGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCG2080.15956916325920015No Hit
TCACTTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA2070.158802003820454No Hit
TCACTTGGGGGAAACTCTGAGTTCCCTCGACTTGTGCAGGTGACCTCAGG2060.15803484438170784No Hit
TCACTTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA2050.1572676849429617No Hit
TCACTTGGGGGGACCGGGAAATCGGGGTCCTACGGAATGTGGAACCACCC2030.1557333660654694No Hit
TCACTTGGGGTGCGAGAAAACCTCAGGCTTTTTGCCAAGATAAAGGGAAT2010.15419904718797708No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCGGGTAGGATTTGCTGAAGGA2010.15419904718797708No Hit
TCACTTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA2010.15419904718797708No Hit
TCACTTGGGTGGGTGTTGTGGTAGGTGCCTGTAGTCCCAACTACTCAGGA1980.1518975688717386No Hit
TCACTTGGGGTGCTTCACTTGGGGGGGGCTGGTGAGATGGCTCAGCAGGT1980.1518975688717386No Hit
TCACTTGGGGAGTGCAGTGCTTCACTTGGGAGGCATTCAGGCAGCGAGAG1960.1503632499942463No Hit
TCACTTGGGTGTTTAATATCATATATGAAATGAGTCGCCAGTCCAGGTTC1960.1503632499942463No Hit
TCACTTGGGGCAGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG1950.14959609055550016No Hit
TCACTTGGGGGATTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCT1900.14576029336176938No Hit
TCACTTGGGAGGTGCTGGAGAGGTGGCTCAGCTGTTAAGAGCACCTACTG1900.14576029336176938No Hit
TCACTTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT1900.14576029336176938No Hit
TCACTTGGGTGCTTCACTTGGGAGCATTTATCTGCAGAGTATGAAAACTT1890.1449931339230232No Hit
TCACTTGGGCGTCTCTACTAAAAATACAAAATTAGCCGGGCGTGGTGGCA1880.14422597448427707No Hit
TCACTTGGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAA1870.1434588150455309No Hit
TCACTTGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT1870.1434588150455309No Hit
TCACTTGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCA1850.1419244961680386No Hit
TCACTTGGGATGGTGTGCATATGTATATGCATCTGTACAGGAACTGAGGG1840.14115733672929245No Hit
TCACTTGGGGTGCAGTGCTTCACTTGGGGACTCTTAGCGGTGGATCACTC1830.1403901772905463No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCCAA1830.1403901772905463No Hit
TCACTTGGGGCATCTGTCTCCTTCAGTTTCTATGTCCCTAGATAACTGGG1790.1373215395355617No Hit
TCACTTGGGGTCTCACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTG1770.13578722065806936No Hit
TCACTTGGGAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGG1770.13578722065806936No Hit
TCACTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1760.1350200612193232No Hit
TCACTTGGACTCACAGGAAGAAATGCACGGCCAAGGCTATGGCTCATTGG1750.13425290178057706No Hit
TCACTTGGGAGGGTGCTGGAGAGGTGGCTCAGCTGTTAAGAGCACCTACT1740.1334857423418309No Hit
TCACTTGGGGAGAGGCTGAGATCCAAGCAGTAACTTCCATCTGTCTCCCT1740.1334857423418309No Hit
TCACTTGGGGCCGGGCTGTGGTGGCACATGCCTTTAATCCCAGCACTTGG1710.13118426402559244No Hit
TCACTTGGGACATACAATAAATAAATTTTAAAAAGAATTTGGACTACTAA1700.1304171045868463No Hit
TCACTTGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGC1680.128882785709354No Hit
TCACTTGGGAAGGCAGGTGAAGCACCAACTGAGAACCCCGCTCCAGCTAC1670.12811562627060782No Hit
TCACTTGGGGCAGACTGTGCCGGACCTGGTGCTGAAACAGACTGAGGCAG1650.12658130739311552No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG1630.1250469885156232Illumina Single End Adapter 2 (100% over 23bp)
TCACTTGGGGCCGGTACTGGTGGCGCAGGCCGGTAGGATTTGCTGAAGGA1630.1250469885156232No Hit
TCACTTGGGTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAAT1620.12427982907687704No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGC1590.12197835076063858No Hit
TCACTTGGGGACATTCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCC1580.12121119132189243No Hit
TCACTTGGGGAGGTGCTGGAGAGGTGGCTCAGCTGTTAAGAGCACCTACT1580.12121119132189243No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCCGA1560.11967687244440013No Hit
TCACTTGGGGCCCTCCCCGCCCCGGGATGTCGGAAGAAACCAGGCAGAGC1530.11737539412816164No Hit
TCACTTGGGCAAAGGAGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.11584107525066933No Hit
TCACTTGGGGCGTGAGTGGTGGTGCGCCTGCGCCCTGTAAACGCGGCGGT1500.1150739158119232No Hit
TCACTTGGGAGTCCATCATTAAGGGAATCCAAGGGAGGAGCTTAGAGCAG1490.11430675637317704No Hit
TCACTTGGGATTGTACCAAAGGCTGAGGAAATGTGTCTTGAGTATCTTCA1490.11430675637317704No Hit
TCACTTGGGGCCCAAACCCAACCTGACCCCACACTGTCACCTTGCTAGCC1490.11430675637317704No Hit
TCACTTGGGAGTTGGGTTTGCAGCCTTCGCCTACACGGTCAAGTACGTGC1480.11353959693443089No Hit
TCACTTGGGACCTGACCTGTGGAAGCTTGGTTACACTAAGACACAGGAAA1470.11277243749568473No Hit
TCACTTGGGACCAGGATGCCGATCAGGACGCCCACCATGGGGTCGATCAC1470.11277243749568473No Hit
TCACTTGGGAGGGTATCAACGCAGAGTCAAATGCTTTTGTTTTATTTTTT1450.11123811861819242No Hit
TCACTTGGGGTCACACCATGGGAGTTTGTTGCACCAGAAGCAGGTAGCTT1450.11123811861819242No Hit
TCACTTGGGAGTGCAGTGCTTCACTTGGATGCACAGAAGGCTTGGAACCG1440.11047095917944626No Hit
TCACTTGGGACGACAGCTAAGACCCAAACTGGGATTAGATACCCCACTAT1440.11047095917944626No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT1430.1097037997407001No Hit
TCACTTGGGGGCGGTCCCTCTCAAACTCACTCTTTGGATCCGTGTTGGGC1420.10893664030195396No Hit
TCACTTGGGGCCGTTGAGTGGGCCCGGAACATCCCCTTCTTCCCTGACCT1410.1081694808632078No Hit
TCACTTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC1410.1081694808632078No Hit
TCACTTGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGG1370.10510084310822318No Hit
TCACTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1360.10433368366947703No Hit
TCACTTGGGGCCCCCCGGGCCGCGGTTTTCCGCGCGGCGCCTCGCCTCGG1360.10433368366947703No Hit
TCACTTGGGGATCTGAAGTAATATATTTCTTCTTCTGAGAAGACATTTTG1340.10279936479198472No Hit
TCACTTGGGGGGCCGCAGCCTGGGTCCTCACCGTCGCCATGGCTCCGTAC1340.10279936479198472No Hit
TCACTTGGGGGGACTCGCTCATGTGTCAGACTGAGCCCTGAGTGTTTGGA1340.10279936479198472No Hit
TCACTTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA1330.10203220535323856No Hit
TCACTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG1320.10126504591449241No Hit
TCACTTGGGGGAGTTGGAAGAGGCGAGTCCGGTCTCAAAATGGAGGGCCA1320.10126504591449241No Hit
TCACTTGGGACATTCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCCG1310.10049788647574626No Hit
TCACTTGGGGGAACGATACAGAGAAGATTAGCATGGCCCCTGCGCAAGGA1310.10049788647574626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTA650.044.00000429
TGGGCAC950.044.0000046
TGATCGA254.4210854E-544.032
CTTGTGC351.4329089E-744.031
TCGCCCC302.5104182E-644.012
ACAACGT207.83079E-444.032
GTCATCC207.83079E-444.030
TATCAGG254.4210854E-544.024
TTCTAGC207.83079E-444.031
AACCGTC207.83079E-444.031
ATGGGCC351.4329089E-744.024
AGACTGT207.83079E-444.012
CGGGTAG302.5104182E-644.030
CGGGTAA351.4329089E-744.030
AAGTAAA207.83079E-444.041
ACAGTTC207.83079E-444.017
TCTGAGT351.4329089E-744.016
TACCCTT207.83079E-444.036
GCCCATT207.83079E-444.021
TCTGACC207.83079E-444.014
Produced by FastQC (version 0.11.5)