FastQCFastQC Report
Fri 17 Jun 2016
SRR1527761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194683
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAG48552.4937976094471526No Hit
TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAA19811.0175516095396104No Hit
TCACTTGGGGAAAGGAAAGAAAGGAAAGAAAGGAAAGAAAGGAAAGAAAGGA11870.6097091168720432No Hit
TCACTTGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA10660.547556797460487No Hit
TCACTTGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGAG10200.5239286429734491No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG9980.512628221262257No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC8730.44842127972139323No Hit
TCACTTGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC8190.42068388097574No Hit
TCACTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA7470.38370068264820245No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6870.3528813507085878No Hit
TCACTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC6390.3282258851568961No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGGAG5980.3071660083314927No Hit
TCACTTGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGA5670.2912426868293585No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.2737783987302435No Hit
TCACTTGGGGAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT5090.26145066595439764No Hit
TCACTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4840.24860927764622487No Hit
TCACTTGGGGGGGAAATCGACTGGGAAGTGCCGAGGATCGCCATCTGTTTCT4790.24604099998459034No Hit
TCACTTGGGGTGCAGTGCTTCACTTGGGATTACTGTAGCTCTCTATAGTGTT4710.24193175572597503No Hit
TCACTTGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG4570.2347405782733983No Hit
TCACTTGGGGTTCGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGG4340.22292650102987932No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC4270.21933091230359097No Hit
TCACTTGGGGAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4140.21265339038334113No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.20648952399541817No Hit
TCACTTGGCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC3890.19981200207516836No Hit
TCACTTGGGCTGGGCTGTGATCCTCTAGCCATCCCGACCTCACACTTCACTA3840.1972437244135338No Hit
TCACTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.18080674737907265No Hit
TCACTTGGGGGGACGCGGAGCGAGAAGCTGAGAGAGTCGGAGACGCTATCCG3500.1797794363144188No Hit
TCACTTGGGATTCAGGCCGGTGCCTACTATGGAATCAAGCCTCTGCCACCTC3480.178752125249765No Hit
TCACTTGGGGTGCGAGAAAACCTCAGGCTTTTTGCCAAGATAAAGGGAATTT3480.178752125249765No Hit
TCACTTGGGAGGTTATTTCTTTGCTTTGCGTTCCCTGGCTCCACCCCCCACG3460.17772481418511119No Hit
TCACTTGGGGAGGAAAACGATCCAGACTATGACCCAAAGAAGGATCAGAACC3460.17772481418511119No Hit
TCACTTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG3430.17618384758813047No Hit
TCACTTGGGGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTA3380.1736155699264959No Hit
TCACTTGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT3360.17258825886184206No Hit
TCACTTGGGACCTGTCTCTGGGCTTTCACTTGGGCAGAGGGAGCCATTTGGT3360.17258825886184206No Hit
TCACTTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC3280.16847901460322678No Hit
TCACTTGGGAAGGCAGGTGAAGCACCAACTGAGAACCCCGCTCCAGCTACCG3270.16796535907089988No Hit
TCACTTGGGGGAAACTCTGAGTTCCCTCGACTTGTGCAGGTGACCTCAGGGG3270.16796535907089988No Hit
TCACTTGGGAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTG3240.16642439247391913No Hit
TCACTTGGGTGGGTGTTGTGGTAGGTGCCTGTAGTCCCAACTACTCAGGAGG3240.16642439247391913No Hit
TCACTTGGGGTGCTTCACTTGGGATGGGCCATATTACTAGGCTTCAGGCAAA3170.16282880374763078No Hit
TCACTTGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACC3140.16128783715065004No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT3100.15923321502134238No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTTCACTTGGGAAG3090.15871955948901548No Hit
TCACTTGGGTGTTTAATATCATATATGAAATGAGTCGCCAGTCCAGGTTCGA3040.15615128182738094No Hit
TCACTTGGGGACTAAGCCCAGTATGAGAGGCGAGTGTGTGTGTGTGTGTGTG3030.155637626295054No Hit
TCACTTGGGGAGTGCAGTGCTTCACTTGGGAGGCATTCAGGCAGCGAGAGCA3010.1546103152304002No Hit
TCACTTGGACTCACAGGAAGAAATGCACGGCCAAGGCTATGGCTCATTGGTG3000.1540966596980733No Hit
TCACTTGGGAGGTGCTGGAGAGGTGGCTCAGCTGTTAAGAGCACCTACTGCT2940.1510147265041118No Hit
TCACTTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA2910.14947375990713108No Hit
TCACTTGGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACG2910.14947375990713108No Hit
TCACTTGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTC2900.14896010437480417No Hit
TCACTTGGGTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATAC2880.14793279331015036No Hit
TCACTTGGGGCATCTGTCTCCTTCAGTTTCTATGTCCCTAGATAACTGGGGT2850.1463918267131696No Hit
TCACTTGGGGGATTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCTGC2830.1453645156485158No Hit
TCACTTGGGCGTCTCTACTAAAAATACAAAATTAGCCGGGCGTGGTGGCATG2810.14433720458386196No Hit
TCACTTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA2750.14125527138990052No Hit
TCACTTGGGTGCTTCACTTGGGAGCATTTATCTGCAGAGTATGAAAACTTTC2740.1407416158575736No Hit
TCACTTGGGACATACAATAAATAAATTTTAAAAAGAATTTGGACTACTAATT2690.13817333819593902No Hit
TCACTTGGGGCGTGAGTGGTGGTGCGCCTGCGCCCTGTAAACGCGGCGGTGG2680.13765968266361212No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCGGGTAGGATTTGCTGAAGGAGG2680.13765968266361212No Hit
TCACTTGGGGAGAGGCTGAGATCCAAGCAGTAACTTCCATCTGTCTCCCTCC2670.1371460271312852No Hit
TCACTTGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGA2660.1366323715989583No Hit
TCACTTGGGGACATTCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCCGC2660.1366323715989583No Hit
TCACTTGGGATGGTGTGCATATGTATATGCATCTGTACAGGAACTGAGGGTG2650.1361187160666314No Hit
TCACTTGGGGTGCTTCACTTGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAA2640.1356050605343045No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCCAAGT2640.1356050605343045No Hit
TCACTTGGGGCCGGTACTGGTGGCGCAGGCCGGTAGGATTTGCTGAAGGAGG2630.13509140500197758No Hit
TCACTTGGGGTCTCACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGAT2600.13355043840499684No Hit
TCACTTGGGGCCGGGCTGTGGTGGCACATGCCTTTAATCCCAGCACTTGGGA2590.13303678287266993No Hit
TCACTTGGGGGGACCGGGAAATCGGGGTCCTACGGAATGTGGAACCACCCAC2590.13303678287266993No Hit
TCACTTGGGGTGCAGTGCTTCACTTGGGGACTCTTAGCGGTGGATCACTCGG2580.13252312734034302No Hit
TCACTTGGGGCAGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT2540.13046850521103537No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGCAG2500.12841388308172774No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTT2490.12790022754940084No Hit
TCACTTGGGGCAGACTGTGCCGGACCTGGTGCTGAAACAGACTGAGGCAGCG2470.126872916484747No Hit
TCACTTGGGAGGGTGCTGGAGAGGTGGCTCAGCTGTTAAGAGCACCTACTGC2470.126872916484747No Hit
TCACTTGGGGGGACTCGCTCATGTGTCAGACTGAGCCCTGAGTGTTTGGAGA2460.1263592609524201No Hit
TCACTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA2450.12584560542009318No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC2430.12481829435543935Illumina Single End Adapter 2 (100% over 25bp)
TCACTTGGGCACCACACCACCACCCCTATCTTCTCCCCCCACCCCATACTCT2390.12276367222613171No Hit
TCACTTGGGACCTGACCTGTGGAAGCTTGGTTACACTAAGACACAGGAAAGC2390.12276367222613171No Hit
TCACTTGGGGCCCTCCCCGCCCCGGGATGTCGGAAGAAACCAGGCAGAGCAA2370.1217363611614779No Hit
TCACTTGGGGCTTGCTATATTAATAAGAAAAAAAGAAAAGAACCAGGCGAGG2360.12122270562915098No Hit
TCACTTGGGAGTGCGGTGCTGTGAGAAGTATCGGTGGGAGCTACGCTGACTC2310.11865442796751642No Hit
TCACTTGGGGCCCAAACCCAACCTGACCCCACACTGTCACCTTGCTAGCCCA2310.11865442796751642No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCCGAGT2280.11711346137053569No Hit
TCACTTGGGGTCACACCATGGGAGTTTGTTGCACCAGAAGCAGGTAGCTTAA2280.11711346137053569No Hit
TCACTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2240.11505883924122805No Hit
TCACTTGGGAGTCCATCATTAAGGGAATCCAAGGGAGGAGCTTAGAGCAGAA2210.11351787264424731No Hit
TCACTTGGGGAACAACCCAAACTCAATTTCCCCCAAAAGCCAAAAATATGGG2190.11249056157959349No Hit
TCACTTGGGAGTTGGGTTTGCAGCCTTCGCCTACACGGTCAAGTACGTGCTG2180.11197690604726658No Hit
TCACTTGGGACCAGGATGCCGATCAGGACGCCCACCATGGGGTCGATCACGG2180.11197690604726658No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT2160.11094959498261275No Hit
TCACTTGGCGCAATTTTTCCCCCTTTTTAATTGAGAGGATCGTTCGAATGAG2140.10992228391795893No Hit
TCACTTGGGAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGG2140.10992228391795893No Hit
TCACTTGGGACGACAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGC2130.10940862838563203No Hit
TCACTTGGGGGCTTCTGCCGACACATGCGCACGGCAGGGCGGGCCCTCTCTC2120.10889497285330513No Hit
TCACTTGGGATTGTACCAAAGGCTGAGGAAATGTGTCTTGAGTATCTTCAGT2110.10838131732097821No Hit
TCACTTGGGACATTCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCCGCC2090.10735400625632438No Hit
TCACTTGGGGATCTGAAGTAATATATTTCTTCTTCTGAGAAGACATTTTGTT2090.10735400625632438No Hit
TCACTTGGGAGTGCAGTGCTTCACTTGGATGCACAGAAGGCTTGGAACCGCT2080.10684035072399747No Hit
TCACTTGGGGCCCCAGGAGGGAAAAGAGTTAAACATAATTACTGAACTACCC2080.10684035072399747No Hit
TCACTTGGGGAGGTGCTGGAGAGGTGGCTCAGCTGTTAAGAGCACCTACTGC2060.10581303965934365No Hit
TCACTTGGGTAGCACACAATATGTTATTATATGCTATTGGCATTGTACAAAC2060.10581303965934365No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA2030.10427207306236291No Hit
TCACTTGGGGAAGGCTTTCCCATGCAGGGAGGGGATGTGCAGTGTGCGGGAG2020.10375841753003599No Hit
TCACTTGGGCCCCCCCAGCAACAATTCACCCACAGTGCACCTGCACATGCCT2010.10324476199770909No Hit
TCACTTGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGC1990.10221745093305529No Hit
TCACTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG1990.10221745093305529No Hit
TCACTTGGGGGGCCGCAGCCTGGGTCCTCACCGTCGCCATGGCTCCGTACCG1960.10067648433607454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTATG351.0137228E-746.00000410
TCGCCCC351.0137228E-746.00000412
CGGAATC551.8189894E-1246.00000438
CGGCGTA351.0137228E-746.00000414
CAGCGGA551.8189894E-1246.00000435
AACCGGG351.0137228E-746.00000431
AACCGCG351.0137228E-746.00000410
AATCCCC551.8189894E-1246.00000441
GAAGCGA351.0137228E-746.00000443
CAAACAA351.0137228E-746.00000424
CTACTAT551.8189894E-1246.00000424
CGAGTGT351.0137228E-746.00000431
GCTGGTT351.0137228E-746.0000049
CCCTATC351.0137228E-746.00000424
GGCGTGA351.0137228E-746.0000049
GGCGTAG351.0137228E-746.00000415
CCAATTG351.0137228E-746.00000429
GCCGGTG551.8189894E-1246.00000416
CCCAGTA351.0137228E-746.00000417
CCTACTA551.8189894E-1246.00000423
Produced by FastQC (version 0.11.5)