FastQCFastQC Report
Fri 17 Jun 2016
SRR1527760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195725
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAG44372.266956188529825No Hit
TCACTTGGGCAAAGGAGGTCATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAA17890.9140375526887213No Hit
TCACTTGGGGAAAGGAAAGAAAGGAAAGAAAGGAAAGAAAGGAAAGAAAGGA11730.5993102567377698No Hit
TCACTTGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA10570.5400434282794737No Hit
TCACTTGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGAG9560.4884404138459573No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG9070.46340528803167713No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGGAG8880.45369779026695617No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC8490.43377187380252913No Hit
TCACTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA7260.3709285987993358No Hit
TCACTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC6680.34129518457018776No Hit
TCACTTGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC6670.3407842636352024No Hit
TCACTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6520.3331204496104228No Hit
TCACTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5820.297355984161451No Hit
TCACTTGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGA5550.2835611189168476No Hit
TCACTTGGGGAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT4730.2416656022480521No Hit
TCACTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4610.2355345510282284No Hit
TCACTTGGGGGGGAAATCGACTGGGAAGTGCCGAGGATCGCCATCTGTTTCT4610.2355345510282284No Hit
TCACTTGGGGTGCAGTGCTTCACTTGGGATTACTGTAGCTCTCTATAGTGTT4290.21918508110869844No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC4160.21254310895388936No Hit
TCACTTGGCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC4070.20794482053902158No Hit
TCACTTGGGGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG3980.2033465321241538No Hit
TCACTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.20079192744922722No Hit
TCACTTGGGGTTCGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGG3820.19517179716438882No Hit
TCACTTGGGGAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3800.1941499552944182No Hit
TCACTTGGGCTGGGCTGTGATCCTCTAGCCATCCCGACCTCACACTTCACTA3770.19261719248946227No Hit
TCACTTGGGATTCAGGCCGGTGCCTACTATGGAATCAAGCCTCTGCCACCTC3460.17677864350491762No Hit
TCACTTGGGGACTAAGCCCAGTATGAGAGGCGAGTGTGTGTGTGTGTGTGTG3300.16860390854515264No Hit
TCACTTGGGGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTA3280.16758206667518202No Hit
TCACTTGGGGGGACGCGGAGCGAGAAGCTGAGAGAGTCGGAGACGCTATCCG3230.16502746200025548No Hit
TCACTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.16451654106527017No Hit
TCACTTGGGGGAAACTCTGAGTTCCCTCGACTTGTGCAGGTGACCTCAGGGG3190.1629837782603142No Hit
TCACTTGGGGTGCTTCACTTGGGATGGGCCATATTACTAGGCTTCAGGCAAA3150.16094009452037297No Hit
TCACTTGGGAGGTTATTTCTTTGCTTTGCGTTCCCTGGCTCCACCCCCCACG3140.16042917358538766No Hit
TCACTTGGGTGGGTGTTGTGGTAGGTGCCTGTAGTCCCAACTACTCAGGAGG3120.15940733171541704No Hit
TCACTTGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACC3100.15838548984544643No Hit
TCACTTGGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACG3080.1573636479754758No Hit
TCACTTGGGGTGCGAGAAAACCTCAGGCTTTTTGCCAAGATAAAGGGAATTT3080.1573636479754758No Hit
TCACTTGGGCGTCTCTACTAAAAATACAAAATTAGCCGGGCGTGGTGGCATG2970.15174351769063738No Hit
TCACTTGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT2970.15174351769063738No Hit
TCACTTGGGAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAAC2950.15072167582066676No Hit
TCACTTGGGGAGGAAAACGATCCAGACTATGACCCAAAGAAGGATCAGAACC2930.1496998339506961No Hit
TCACTTGGGTGTTTAATATCATATATGAAATGAGTCGCCAGTCCAGGTTCGA2910.1486779920807255No Hit
TCACTTGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG2910.1486779920807255No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGCAG2890.14765615021075487No Hit
TCACTTGGGGGATTCTCTCTCGCTGGCATCTCTCTGGCTCTGCGCGCCCTGC2880.14714522927576956No Hit
TCACTTGGGACCTGTCTCTGGGCTTTCACTTGGGCAGAGGGAGCCATTTGGT2880.14714522927576956No Hit
TCACTTGGGAAGGCAGGTGAAGCACCAACTGAGAACCCCGCTCCAGCTACCG2860.14612338740579894No Hit
TCACTTGGGAGGTGCTGGAGAGGTGGCTCAGCTGTTAAGAGCACCTACTGCT2800.1430578617958871No Hit
TCACTTGGGGTGCTTCACTTGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAA2780.14203601992591647No Hit
TCACTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA2780.14203601992591647No Hit
TCACTTGGGTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATAC2770.14152509899093116No Hit
TCACTTGGGACATACAATAAATAAATTTTAAAAAGAATTTGGACTACTAATT2750.14050325712096054No Hit
TCACTTGGGGCCGGGCTGTGGTGGCACATGCCTTTAATCCCAGCACTTGGGA2710.1384595733810193No Hit
TCACTTGGACTCACAGGAAGAAATGCACGGCCAAGGCTATGGCTCATTGGTG2700.137948652446034No Hit
TCACTTGGGGGGACCGGGAAATCGGGGTCCTACGGAATGTGGAACCACCCAC2690.13743773151104868No Hit
TCACTTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA2690.13743773151104868No Hit
TCACTTGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTC2670.13641588964107806No Hit
TCACTTGGGAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTG2650.13539404777110742No Hit
TCACTTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA2650.13539404777110742No Hit
TCACTTGGGGCATCTGTCTCCTTCAGTTTCTATGTCCCTAGATAACTGGGGT2620.1338612849661515No Hit
TCACTTGGGCACCACACCACCACCCCTATCTTCTCCCCCCACCCCATACTCT2570.13130668029122494No Hit
TCACTTGGGTGCTTCACTTGGGAGCATTTATCTGCAGAGTATGAAAACTTTC2570.13130668029122494No Hit
TCACTTGGGGTCAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGA2550.13028483842125432No Hit
TCACTTGGGATGGTGTGCATATGTATATGCATCTGTACAGGAACTGAGGGTG2530.12926299655128368No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCGGGTAGGATTTGCTGAAGGAGG2510.12824115468131306No Hit
TCACTTGGGGAGGTGCTGGAGAGGTGGCTCAGCTGTTAAGAGCACCTACTGC2510.12824115468131306No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT2500.12773023374632775No Hit
TCACTTGGGGAGAGGCTGAGATCCAAGCAGTAACTTCCATCTGTCTCCCTCC2500.12773023374632775No Hit
TCACTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATCTGCCTCCCAAGT2440.12466470813641589No Hit
TCACTTGGGGTCTCACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGAT2420.12364286626644527No Hit
TCACTTGGGGAGTGCAGTGCTTCACTTGGGAGGCATTCAGGCAGCGAGAGCA2400.12262102439647465No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTTCACTTGGGAAG2390.12211010346148933No Hit
TCACTTGGGGCGTGAGTGGTGGTGCGCCTGCGCCCTGTAAACGCGGCGGTGG2360.1205773406565334No Hit
TCACTTGGGGCAGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT2360.1205773406565334No Hit
TCACTTGGGGACATTCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCCGC2340.11955549878656278No Hit
TCACTTGGGAGGGTGCTGGAGAGGTGGCTCAGCTGTTAAGAGCACCTACTGC2330.11904457785157747No Hit
TCACTTGGGGCCCAAACCCAACCTGACCCCACACTGTCACCTTGCTAGCCCA2310.11802273598160685No Hit
TCACTTGGGAGTGCAGTGCTTCACTTGGATGCACAGAAGGCTTGGAACCGCT2300.11751181504662155No Hit
TCACTTGGGATTGTACCAAAGGCTGAGGAAATGTGTCTTGAGTATCTTCAGT2260.11546813130668031No Hit
TCACTTGGGGTGCAGTGCTTCACTTGGGGACTCTTAGCGGTGGATCACTCGG2250.11495721037169497No Hit
TCACTTGGGAGTGCGGTGCTGTGAGAAGTATCGGTGGGAGCTACGCTGACTC2230.11393536850172435No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTT2230.11393536850172435No Hit
TCACTTGGGGCAGACTGTGCCGGACCTGGTGCTGAAACAGACTGAGGCAGCG2200.11240260569676842No Hit
TCACTTGGGGTCACACCATGGGAGTTTGTTGCACCAGAAGCAGGTAGCTTAA2200.11240260569676842No Hit
TCACTTGGGGCCGGTACTGGTGGCGCAGGCCGGTAGGATTTGCTGAAGGAGG2180.1113807638267978No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC2180.1113807638267978Illumina Single End Adapter 2 (100% over 25bp)
TCACTTGGGAGTCCATCATTAAGGGAATCCAAGGGAGGAGCTTAGAGCAGAA2160.11035892195682719No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGTAG2090.10678247541192999No Hit
TCACTTGGGGCTTGCTATATTAATAAGAAAAAAAGAAAAGAACCAGGCGAGG2090.10678247541192999No Hit
TCACTTGGGGCCCCCCGGGCCGCGGTTTTCCGCGCGGCGCCTCGCCTCGGCC2080.10627155447694468No Hit
TCACTTGGGGATCTGAAGTAATATATTTCTTCTTCTGAGAAGACATTTTGTT2070.10576063354195937No Hit
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT2060.10524971260697406No Hit
TCACTTGGGGCCCTCCCCGCCCCGGGATGTCGGAAGAAACCAGGCAGAGCAA2030.10371694980201814No Hit
TCACTTGGGTAGCACACAATATGTTATTATATGCTATTGGCATTGTACAAAC2010.10269510793204752No Hit
TCACTTGGGGGGCCGCAGCCTGGGTCCTCACCGTCGCCATGGCTCCGTACCG2000.1021841869970622No Hit
TCACTTGGGACCTGACCTGTGGAAGCTTGGTTACACTAAGACACAGGAAAGC1980.10116234512709157No Hit
TCACTTGGGACATTCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCCGCC1970.10065142419210626No Hit
TCACTTGGGAGTTGGGTTTGCAGCCTTCGCCTACACGGTCAAGTACGTGCTG1960.10014050325712096No Hit
TCACTTGGGCTGGTTTTATAGATCACCCCCAATTGTATGGCTTAAAACAAGC1960.10014050325712096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATG351.0137592E-746.00000421
CGGGTAG351.0137592E-746.00000430
ATGGCTA351.0137592E-746.00000442
TACCAAA351.0137592E-746.00000414
CGCGGGT351.0137592E-746.00000428
GAGTATC351.0137592E-746.00000440
CCCAGTA351.0137592E-746.00000417
CACGCGG351.0137592E-746.00000426
GTGATCC351.0137592E-746.00000417
TTCGTCT351.0137592E-746.00000437
CGTTCGT351.0137592E-746.00000435
TGTGATC351.0137592E-746.00000416
CTAAACG351.0137592E-746.00000446
CCTCACA351.0137592E-746.00000438
ATCCCGA351.0137592E-746.00000431
GATATGG351.0137592E-746.00000422
TGCCTCG351.0137592E-746.00000431
AGACACA351.0137592E-746.00000439
GAGCCGT351.0137592E-746.00000431
TCCCGAC351.0137592E-746.00000432
Produced by FastQC (version 0.11.5)