FastQCFastQC Report
Fri 17 Jun 2016
SRR1527759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488071
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC36440.746612685449453No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG26780.5486906618094499No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC25450.5214405281198842No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG16920.34667087370485034No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC16430.3366313507665893No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT12410.25426628502820287No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT11540.23644100960720876No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG11080.22701615133863723No Hit
ACGAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA10400.21308375215900965No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT9870.20222467632782937No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC9650.1977171354167734No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTGCT9650.1977171354167734No Hit
ACGAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC9430.1932095945057174No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC8690.17804786598671096No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA8510.17435987796857425No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG8280.16964744883428845No Hit
ACGAGCGGGGGGCTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGAC7770.15919814944956778No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT7750.15878837300310816No Hit
ACGAGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC7450.15264172630621364No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG7410.15182217341329438No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT6880.14096309758211406No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC6790.13911910357304572No Hit
ACGAGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC6280.12866980418832505No Hit
ACGAGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG6210.12723558662571635No Hit
ACGAGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT6190.1268258101792567No Hit
ACGAGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG5960.12211338104497092No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT5780.1184253930268342No Hit
ACGAGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC5690.11658139901776585No Hit
ACGAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA5680.11637651079453604No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCT5530.11330318744608879No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC5420.11104941699056081No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC5390.11043475232087134No Hit
ACGAGCGGGGGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTTG5290.10838587008857319No Hit
ACGAGCGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG5270.10797609364211354No Hit
ACGAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC5220.10695165252596446No Hit
ACGAGCGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGG5030.10305877628459795No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACCGACTGCT5000.10244411161490848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG302.5247118E-644.017
AAACTCG254.4390632E-544.019
CGGGTAT502.7284841E-1144.06
TCGTTTG254.4390632E-544.040
TGATAGG254.4390632E-544.041
TGATAAC254.4390632E-544.029
TCGCAAG207.8520266E-444.036
ACACGAA254.4390632E-544.039
ATTAGTG254.4390632E-544.037
GTATTGA302.5247118E-644.015
GATAAGG207.8520266E-444.016
TACTTGT254.4390632E-544.013
CTATCTA302.5247118E-644.013
CGCGGAT254.4390632E-544.019
CCGATAT207.8520266E-444.015
GCGCGTA207.8520266E-444.039
CTATAGG302.5247118E-644.023
CTATAGC254.4390632E-544.028
CGGCATA254.4390632E-544.013
AACTTAA254.4390632E-544.022
Produced by FastQC (version 0.11.5)