##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527759_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	488071
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.90735978986664	33.0	31.0	34.0	3.0	34.0
2	30.343269729199235	34.0	31.0	34.0	16.0	34.0
3	32.199845924056135	34.0	31.0	34.0	28.0	34.0
4	36.066219873747876	37.0	35.0	37.0	35.0	37.0
5	36.18013772586365	37.0	35.0	37.0	35.0	37.0
6	36.23200517957428	37.0	37.0	37.0	35.0	37.0
7	36.55291340809022	37.0	37.0	37.0	35.0	37.0
8	36.67682160997068	37.0	37.0	37.0	35.0	37.0
9	38.76416750841578	39.0	39.0	39.0	39.0	39.0
10	38.29425431955597	39.0	39.0	39.0	37.0	39.0
11	37.964839132011534	39.0	38.0	39.0	35.0	39.0
12	37.09860655519381	39.0	37.0	39.0	34.0	39.0
13	36.99087018077288	39.0	37.0	39.0	33.0	39.0
14	37.716020824839006	40.0	37.0	40.0	33.0	40.0
15	37.70310057348214	40.0	36.0	40.0	33.0	40.0
16	37.65923605377087	40.0	36.0	40.0	33.0	40.0
17	37.52767322787054	40.0	36.0	40.0	33.0	40.0
18	37.37684886010437	40.0	36.0	40.0	33.0	40.0
19	37.29057657594899	39.0	35.0	40.0	33.0	40.0
20	37.08480118671259	39.0	35.0	40.0	32.0	40.0
21	36.97809949781897	39.0	35.0	40.0	32.0	40.0
22	36.99588584447755	39.0	35.0	40.0	32.0	40.0
23	36.861686107144244	39.0	35.0	40.0	32.0	40.0
24	36.74069551356258	38.0	35.0	40.0	32.0	40.0
25	36.66574125485841	38.0	35.0	40.0	32.0	40.0
26	36.491858766449965	38.0	35.0	40.0	32.0	40.0
27	36.29054584271551	38.0	35.0	40.0	32.0	40.0
28	35.97732092257069	37.0	34.0	40.0	31.0	40.0
29	36.04319863298578	37.0	35.0	40.0	31.0	40.0
30	36.20285982981984	37.0	35.0	40.0	32.0	40.0
31	36.14151219802037	37.0	35.0	40.0	32.0	40.0
32	36.09076548289081	37.0	35.0	40.0	32.0	40.0
33	35.88419102958381	36.0	35.0	40.0	32.0	40.0
34	35.43999336162157	36.0	34.0	40.0	31.0	40.0
35	35.36284270116438	35.0	34.0	40.0	30.0	40.0
36	35.36412939920626	35.0	34.0	40.0	31.0	40.0
37	35.319973938218006	35.0	34.0	40.0	31.0	40.0
38	35.25722077320718	35.0	34.0	39.0	31.0	40.0
39	35.121814244239054	35.0	34.0	39.0	31.0	40.0
40	34.89550085950609	35.0	34.0	39.0	30.0	40.0
41	34.764747342087524	35.0	34.0	39.0	30.0	40.0
42	34.66221922630109	35.0	34.0	39.0	30.0	40.0
43	34.57115255772214	35.0	34.0	38.0	30.0	40.0
44	34.226614570421106	35.0	34.0	37.0	29.0	40.0
45	34.25684992552313	35.0	34.0	37.0	29.0	40.0
46	34.00221484169312	35.0	34.0	37.0	29.0	40.0
47	34.01162535778606	35.0	34.0	37.0	29.0	40.0
48	33.93314702164234	35.0	34.0	37.0	29.0	40.0
49	33.881324643340825	35.0	34.0	36.0	29.0	40.0
50	33.38833489389863	35.0	33.0	36.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	4.0
14	5.0
15	10.0
16	23.0
17	43.0
18	110.0
19	186.0
20	357.0
21	524.0
22	814.0
23	1117.0
24	1563.0
25	2093.0
26	2816.0
27	3906.0
28	5380.0
29	7171.0
30	9616.0
31	12753.0
32	17567.0
33	28379.0
34	53765.0
35	70534.0
36	84209.0
37	79221.0
38	77576.0
39	28328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.04952353243688	0.7277629689123098	0.1628861374677045	0.05982736118310656
8	99.57547160146783	0.24545609142932073	0.06966199589813776	0.10941031120472225
9	98.74608407383352	0.653798320326346	0.22086950464174268	0.3792481011983912
10	62.76218009265045	28.653413130466674	2.7645567960399204	5.819849980842951
11	41.43372583087297	22.639943778671544	19.20970514535795	16.71662524509754
12	34.73183204902565	14.36102534262433	25.039184872692704	25.86795773565731
13	27.05344099526503	15.401652628408572	27.868896123719704	29.676010252606687
14	26.798560045567143	16.29885815793194	29.923310338045077	26.979271458455838
15	27.97523311157598	17.56301849525991	28.629236320125557	25.832512073038554
16	34.34561774823745	18.70793388666813	24.399933616215673	22.54651474887875
17	33.183081969631466	19.905710439669637	23.168555394604475	23.742652196094422
18	31.29134900455057	20.984242046751394	22.688092511130552	25.036316437567486
19	31.55626947718672	21.52842516764979	21.998848528185448	24.91645682697804
20	31.642527419166473	22.865115936001114	21.822644656207807	23.669711988624606
21	31.449727601107213	21.790886981607187	23.69819145165355	23.06119396563205
22	31.790251828115174	18.773907894548127	25.642990466550973	23.792849810785725
23	32.32152699095009	20.23783424952517	23.55456480716945	23.88607395235529
24	31.25569845370858	19.429959985330004	22.848315101696272	26.466026459265144
25	29.582376334590666	20.609091710017598	21.4927746168078	28.315757338583936
26	26.546342642771236	20.984651823197854	23.534895537739388	28.934109996291525
27	27.232513302367895	19.23162818524354	22.897898051717885	30.637960460670683
28	28.495239422133256	22.37871129405353	21.195891581347794	27.93015770246542
29	31.07908480528448	21.93922605522557	21.04693784305972	25.934751296430232
30	29.642408583997	18.96527349504478	23.634061437782616	27.758256483175604
31	32.61287804438289	20.2380391377484	20.569548282934242	26.579534534934467
32	34.83898858977485	19.717418162521437	22.553890724915025	22.889702522788692
33	34.17330675250117	18.642984319904276	24.947804725132205	22.235904202462347
34	28.651569136457606	21.067426665382698	28.52740687318034	21.753597324979356
35	27.320615238356716	26.25949913024949	26.72357095586503	19.696314675528765
36	29.97903993476359	26.37935874083894	22.640353555118008	21.00124776927947
37	30.069600529431167	26.839537690213106	20.070235682923183	23.020626097432544
38	31.235824296055288	26.47401709997111	20.870324194635618	21.419834409337984
39	31.41878947939951	22.911010898004594	21.126229585449657	24.543970037146234
40	28.641324725296112	23.573414523706592	20.26180617164306	27.523454579354233
41	26.627683267393476	24.349940889747597	21.095906128411645	27.926469714447283
42	24.692718887211083	22.44570974304968	21.895175087231163	30.96639628250808
43	25.371513570771466	20.43063406758443	24.412431797832692	29.78542056381141
44	28.338704819585676	19.32239366813435	23.397005763505717	28.941895748774254
45	28.73147554351723	22.320727926879492	22.593229263775147	26.354567265828127
46	27.767681341444174	22.594868369560984	21.993316546158244	27.644133742836598
47	26.143532395901413	22.7262017206513	24.320232097379275	26.81003378606801
48	28.532529078761083	20.80885772766667	25.131179684922888	25.527433508649356
49	28.085872752120082	21.108609198251894	24.09895281629107	26.706565233336953
50	26.577075876255705	21.111067856930653	24.508729262750705	27.803127004062933
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.5
8	7.0
9	4.0
10	1.0
11	1.0
12	1.0
13	19.0
14	37.0
15	249.5
16	462.0
17	701.0
18	940.0
19	753.0
20	566.0
21	579.0
22	592.0
23	692.5
24	793.0
25	718.0
26	643.0
27	847.0
28	1051.0
29	1517.5
30	1984.0
31	2065.5
32	2147.0
33	2720.0
34	3293.0
35	3234.0
36	3175.0
37	5144.0
38	7113.0
39	6720.5
40	6328.0
41	6625.5
42	6923.0
43	8675.5
44	10428.0
45	11109.0
46	11790.0
47	12585.0
48	13380.0
49	14687.0
50	15994.0
51	17011.5
52	18029.0
53	20147.0
54	22265.0
55	23877.5
56	25490.0
57	28218.5
58	30947.0
59	37746.0
60	44545.0
61	48259.0
62	51973.0
63	50141.0
64	48309.0
65	49370.5
66	50432.0
67	43945.5
68	37459.0
69	30374.5
70	23290.0
71	20642.5
72	17995.0
73	14080.0
74	10165.0
75	8376.5
76	6588.0
77	7413.5
78	8239.0
79	5685.5
80	3132.0
81	2062.0
82	992.0
83	702.5
84	413.0
85	259.5
86	106.0
87	63.5
88	21.0
89	18.5
90	16.0
91	14.5
92	13.0
93	7.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	488071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.518082410141147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.48132601442256	6.518109045610167
2	9.62652028845119	1.832520268567483
3	3.5625874502206436	1.0172700283360414
4	1.651060165751803	0.6285970688690785
5	0.9363900548918308	0.44563188552485195
6	0.6156495533311807	0.35158819106236594
7	0.4972554084597998	0.33130425696261406
8	0.38531912603594876	0.2933999356650979
9	0.3035195350339038	0.26000315527863777
>10	7.7666559035625875	21.431308149838856
>50	4.161016037025078	27.435967308035103
>100	1.935206113443117	32.19060341630623
>500	0.05812076202776881	3.832843991960186
>1k	0.019373587342589604	3.430853297983285
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3644	0.746612685449453	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	2678	0.5486906618094499	No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2545	0.5214405281198842	No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1692	0.34667087370485034	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1643	0.3366313507665893	No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1241	0.25426628502820287	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1154	0.23644100960720876	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1108	0.22701615133863723	No Hit
ACGAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	1040	0.21308375215900965	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	987	0.20222467632782937	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	965	0.1977171354167734	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTGCT	965	0.1977171354167734	No Hit
ACGAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	943	0.1932095945057174	No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	869	0.17804786598671096	No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	851	0.17435987796857425	No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	828	0.16964744883428845	No Hit
ACGAGCGGGGGGCTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGAC	777	0.15919814944956778	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	775	0.15878837300310816	No Hit
ACGAGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	745	0.15264172630621364	No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	741	0.15182217341329438	No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	688	0.14096309758211406	No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	679	0.13911910357304572	No Hit
ACGAGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	628	0.12866980418832505	No Hit
ACGAGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	621	0.12723558662571635	No Hit
ACGAGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	619	0.1268258101792567	No Hit
ACGAGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	596	0.12211338104497092	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	578	0.1184253930268342	No Hit
ACGAGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC	569	0.11658139901776585	No Hit
ACGAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	568	0.11637651079453604	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCT	553	0.11330318744608879	No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	542	0.11104941699056081	No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	539	0.11043475232087134	No Hit
ACGAGCGGGGGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTTG	529	0.10838587008857319	No Hit
ACGAGCGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	527	0.10797609364211354	No Hit
ACGAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	522	0.10695165252596446	No Hit
ACGAGCGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGG	503	0.10305877628459795	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACCGACTGCT	500	0.10244411161490848	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.005327093803975241	0.0	0.0	0.0	0.0
8	0.00676131136658396	0.0	0.0	0.0	0.0
9	0.009015081822111947	0.0	0.0	0.0	0.0
10	0.01352262273316792	0.0	0.0	0.0	0.0
11	0.0217181516623606	0.0	0.0	0.0	0.0
12	0.02704524546633584	0.0	0.0	0.0	0.0
13	0.03585543906521797	0.0	0.0	0.0	0.0
14	0.03995320352981431	0.0	0.0	0.0	0.0
15	0.04814873245900699	0.0	0.0	0.0	0.0
16	0.053885602709441864	0.0	0.0	0.0	0.0
17	0.06535934321031162	0.0	0.0	0.0	0.0
18	0.07334998391627448	0.0	0.0	0.0	0.0
19	0.08236506573838642	0.0	0.0	0.0	0.0
20	0.08953615355143002	0.0	0.0	0.0	0.0
21	0.09732190603416306	0.0	0.0	0.0	0.0
22	0.10551743496335574	0.0	0.0	0.0	0.0
23	0.1147374050086975	0.0	0.0	0.0	0.0
24	0.12170360459851129	0.0	0.0	0.0	0.0
25	0.13235779220646177	0.0	0.0	0.0	0.0
26	0.1409630975821141	0.0	0.0	0.0	0.0
27	0.14915862651130676	0.0	0.0	0.0	0.0
28	0.15837859655664852	0.0	0.0	0.0	0.0
29	0.169032784164599	0.0	0.0	0.0	0.0
30	0.1762038719776426	0.0	0.0	0.0	0.0
31	0.18276029512099673	0.0	0.0	0.0	0.0
32	0.19279981805925778	0.0	0.0	0.0	0.0
33	0.19956112942584173	0.0	0.0	0.0	0.0
34	0.21042020525702204	0.0	0.0	0.0	0.0
35	0.22250861042758124	0.0	0.0	0.0	0.0
36	0.23603123316074914	0.0	0.0	0.0	0.0
37	0.24996363234037672	0.0	0.0	0.0	0.0
38	0.2620520375109359	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	30	2.5247118E-6	44.0	17
AAACTCG	25	4.4390632E-5	44.0	19
CGGGTAT	50	2.7284841E-11	44.0	6
TCGTTTG	25	4.4390632E-5	44.0	40
TGATAGG	25	4.4390632E-5	44.0	41
TGATAAC	25	4.4390632E-5	44.0	29
TCGCAAG	20	7.8520266E-4	44.0	36
ACACGAA	25	4.4390632E-5	44.0	39
ATTAGTG	25	4.4390632E-5	44.0	37
GTATTGA	30	2.5247118E-6	44.0	15
GATAAGG	20	7.8520266E-4	44.0	16
TACTTGT	25	4.4390632E-5	44.0	13
CTATCTA	30	2.5247118E-6	44.0	13
CGCGGAT	25	4.4390632E-5	44.0	19
CCGATAT	20	7.8520266E-4	44.0	15
GCGCGTA	20	7.8520266E-4	44.0	39
CTATAGG	30	2.5247118E-6	44.0	23
CTATAGC	25	4.4390632E-5	44.0	28
CGGCATA	25	4.4390632E-5	44.0	13
AACTTAA	25	4.4390632E-5	44.0	22
>>END_MODULE