FastQCFastQC Report
Fri 17 Jun 2016
SRR1527758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621563
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC44520.7162588506716133No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT31870.5127396579268715No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT29370.4725184735899659No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA22010.3541073069021161No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG21510.346063070034735No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG16900.27189520611748125No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT14300.23006517440709953No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT13490.21703351068194213No Hit
ACGAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG13400.21558554804581354No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC13200.2123678532988611No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC12700.20432361643148No Hit
ACGAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC12310.19804911167492276No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTGCTCT12240.1969229185134894No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT11520.18533921742446058No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11160.17954736687994619No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG10770.17327286212338894No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC10490.1687680894776555No Hit
ACGAGCGGGGGGCTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG9890.1591150052367982No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG9710.15621907996454099No Hit
ACGAGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT9510.15300138521758855No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA8620.1386826435936502No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT8320.13385610147322155No Hit
ACGAGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA7870.12661628829257857No Hit
ACGAGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC7850.1262945188178833No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC7670.1233985935456261No Hit
ACGAGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC7660.12323770880827847No Hit
ACGAGCGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT7500.12066355301071653No Hit
ACGAGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG7460.12002001406132604No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGC7210.11599789562763549No Hit
ACGAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG7080.11390639404211642No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG6980.11229754666864018No Hit
ACGAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT6980.11229754666864018No Hit
ACGAGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC6780.10907985192168775No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACCGACTGCTCT6750.10859719770964488No Hit
ACGAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT6530.10505773348799718No Hit
ACGAGCGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG6480.10425330980125909No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA6250.10055296084226378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGTA351.0185613E-746.00000428
TCGACGC351.0185613E-746.00000435
TCAACGA351.0185613E-746.00000413
GCATAAC206.309206E-446.031
TCACGAC253.415332E-546.024
CTTAACG551.8189894E-1246.017
CGTGGAA206.309206E-446.021
ACTATCG206.309206E-446.045
GGTACTC206.309206E-446.08
ACTATAG206.309206E-446.035
GATACGA253.415332E-546.034
TCTGACG206.309206E-446.043
GTCGCAA301.8600131E-646.010
TCGCAAA301.8600131E-646.011
CGAACGT301.8600131E-646.038
ACAAAAC206.309206E-446.034
CGTATTG253.415332E-546.013
AATTTCG206.309206E-446.031
ATTAGGT206.309206E-446.027
CGTATAT206.309206E-446.028
Produced by FastQC (version 0.11.5)