##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527758_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	621563
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.209874139869974	33.0	31.0	34.0	31.0	34.0
2	32.631327797825804	34.0	31.0	34.0	31.0	34.0
3	32.74814137907179	34.0	31.0	34.0	31.0	34.0
4	36.212856942900395	37.0	37.0	37.0	35.0	37.0
5	36.12757033478505	37.0	37.0	37.0	35.0	37.0
6	35.94725072116584	37.0	37.0	37.0	35.0	37.0
7	36.498282555428815	37.0	37.0	37.0	35.0	37.0
8	36.63870436303319	37.0	37.0	37.0	35.0	37.0
9	38.70829505617291	39.0	39.0	39.0	39.0	39.0
10	38.057746358776186	39.0	38.0	39.0	37.0	39.0
11	37.664568193409195	39.0	38.0	39.0	35.0	39.0
12	36.802295181663	39.0	35.0	39.0	33.0	39.0
13	36.70662024605712	39.0	35.0	39.0	33.0	39.0
14	37.44776313905429	40.0	36.0	40.0	33.0	40.0
15	37.47953626583307	40.0	36.0	40.0	33.0	40.0
16	37.463636027241	40.0	35.0	40.0	33.0	40.0
17	37.35753093411287	40.0	35.0	40.0	33.0	40.0
18	37.3037873876019	40.0	35.0	40.0	33.0	40.0
19	37.24996339872225	39.0	35.0	40.0	33.0	40.0
20	37.116202219244066	39.0	35.0	40.0	32.0	40.0
21	36.98959880173047	39.0	35.0	40.0	32.0	40.0
22	36.93633951827892	39.0	35.0	40.0	32.0	40.0
23	36.84124859426961	39.0	35.0	40.0	32.0	40.0
24	36.708549254057914	38.0	35.0	40.0	32.0	40.0
25	36.64902833662879	38.0	35.0	40.0	32.0	40.0
26	36.468047486739074	38.0	35.0	40.0	32.0	40.0
27	36.31377350324907	38.0	35.0	40.0	32.0	40.0
28	36.06526450255244	37.0	35.0	40.0	31.0	40.0
29	35.89649158653266	37.0	35.0	40.0	31.0	40.0
30	35.73588035323853	37.0	34.0	40.0	31.0	40.0
31	35.51201889430355	36.0	34.0	40.0	30.0	40.0
32	35.37140241616699	36.0	34.0	40.0	30.0	40.0
33	35.13881135138353	35.0	34.0	39.0	30.0	40.0
34	34.78980730835008	35.0	33.0	39.0	28.0	40.0
35	34.60489121778484	35.0	33.0	39.0	28.0	40.0
36	34.64734548227613	35.0	33.0	39.0	29.0	40.0
37	34.60929302419867	35.0	34.0	39.0	29.0	40.0
38	34.52909520032563	35.0	34.0	39.0	29.0	40.0
39	34.40047750590045	35.0	33.0	38.0	29.0	40.0
40	34.13640129801806	35.0	33.0	38.0	28.0	40.0
41	34.006734635105374	35.0	33.0	38.0	27.0	40.0
42	33.92527386604415	35.0	33.0	37.0	27.0	40.0
43	33.816784461108526	35.0	33.0	37.0	27.0	40.0
44	33.6837778310485	35.0	33.0	37.0	27.0	40.0
45	33.60325341115864	35.0	33.0	37.0	27.0	40.0
46	33.37921176131784	35.0	33.0	36.0	26.0	40.0
47	33.258100948737294	35.0	33.0	36.0	26.0	40.0
48	33.05833037037275	35.0	32.0	36.0	25.0	39.0
49	33.42380257512111	35.0	33.0	36.0	27.0	39.0
50	33.5662193534686	35.0	33.0	36.0	27.0	39.0
51	33.614467077351776	35.0	33.0	36.0	28.0	39.0
52	33.177335201741414	35.0	33.0	35.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	1.0
15	10.0
16	27.0
17	71.0
18	143.0
19	314.0
20	527.0
21	816.0
22	1282.0
23	1933.0
24	2725.0
25	3678.0
26	5041.0
27	6646.0
28	8768.0
29	11096.0
30	14127.0
31	18389.0
32	24097.0
33	35067.0
34	70373.0
35	93043.0
36	107908.0
37	89547.0
38	97685.0
39	28245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.09309273557145	0.7088581527536227	0.1504272294200266	0.04762188225489612
8	99.65409781470261	0.24100533654673784	0.03861233696342929	0.06628451178722028
9	98.791594737782	0.6565706131156456	0.20464538590617526	0.3471892631961684
10	62.72622405130293	28.65662853162109	2.8158046730580812	5.801342744017903
11	41.40980077642975	22.69391839604352	19.236344505705777	16.659936321820958
12	34.88512025329693	14.279163978550846	25.012267461222752	25.823448306929464
13	27.126775564182555	15.287911281720437	27.904975038733	29.68033811536401
14	26.812728556879996	16.473470911235065	29.70575790386493	27.008042628020007
15	28.059102617112025	17.431539522140152	28.68365716749549	25.825700693252333
16	34.40938408496001	18.738567128352233	24.399457496665665	22.452591290022088
17	33.265976256630466	19.791557734292418	22.99380754645949	23.94865846261763
18	31.29256406832453	21.000606535459802	22.796884627946	24.909944768269668
19	31.58344367344903	21.51205911548789	22.068720306710663	24.835776904352414
20	31.488682563151283	22.89840289721235	21.95400948898181	23.65890505065456
21	31.490130525787407	21.797146870067877	23.786325762633876	22.926396841510837
22	31.813187078381432	18.674213233413187	25.626364503678627	23.88623518452675
23	32.29439332778817	20.236082263583903	23.55256023926778	23.916964169360146
24	31.266822510348913	19.33754100549743	22.83823200544434	26.55740447870932
25	29.54567759020405	20.491406341754576	21.544879601906803	28.418036466134566
26	26.412608215096455	21.11097346528027	23.465843365837415	29.010574953785863
27	27.091702691440773	19.19837570769174	22.844023855988855	30.865897744878634
28	28.54642248653797	22.378262541367487	21.344095449696972	27.73121952239757
29	30.897270268661426	21.911535918322038	21.000606535459802	26.190587277556745
30	29.48743731528421	18.982146620696536	23.733072914571814	27.79734314944744
31	32.7424573213013	20.162879708090735	20.469043363263257	26.62561960734471
32	34.798886034078606	19.726399415666634	22.40239525196963	23.07231929828513
33	34.18607606952151	18.56432895780476	25.07404720036424	22.175547772309486
34	28.530494897540553	20.930460789976237	28.645849254218803	21.893195058264407
35	27.240842842962014	26.35324174701519	26.910224707712654	19.495690702310146
36	29.96767825626686	26.238048275074288	22.689574508135138	21.104698960523713
37	30.12228848885793	26.704614013382393	20.160788206505213	23.012309291254464
38	31.02967840749852	26.693673851242757	20.759118544701018	21.51752919655771
39	31.37992448070429	22.912399869361593	21.17307497389645	24.534600676037666
40	28.63249582101895	23.610961398924967	20.13665549590307	27.619887284153016
41	26.52072275859406	24.509824426486134	21.08925402573834	27.880198789181463
42	24.641106372161794	22.503430867023937	21.87951985558986	30.975942905224414
43	25.351412487551546	20.524548597648188	24.394470069807888	29.729568844992382
44	28.087900985097246	19.415730987848377	23.30174093374284	29.19462709331154
45	28.781957742014892	22.33144508279933	22.574863690406282	26.3117334847795
46	27.423768789326264	22.603662058391507	22.16042460699881	27.812144545283424
47	26.266203104110126	22.62522061319609	24.158452160118927	26.950124122574863
48	28.515210847492533	20.82669013438702	25.203720298666425	25.454378719454024
49	28.17284812641679	21.00108918967184	24.173253555954908	26.652809127956456
50	26.354689709651314	21.118695932672953	24.499688688033235	28.026925669642498
51	26.305298095285597	20.170763060220768	24.779949900492788	28.743988944000847
52	26.41727387247954	20.644407726972165	26.2407833156092	26.697535084939094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	8.0
8	16.0
9	10.5
10	5.0
11	4.0
12	3.0
13	24.0
14	292.0
15	539.0
16	860.5
17	1182.0
18	867.0
19	552.0
20	667.5
21	783.0
22	834.5
23	886.0
24	833.5
25	781.0
26	845.5
27	910.0
28	1269.5
29	1629.0
30	2131.0
31	2633.0
32	3131.5
33	3630.0
34	3522.0
35	3414.0
36	4599.0
37	5784.0
38	6522.5
39	8084.5
40	8908.0
41	10045.0
42	11182.0
43	10872.0
44	10562.0
45	12625.0
46	14688.0
47	16934.5
48	19181.0
49	17916.0
50	16651.0
51	20367.0
52	24083.0
53	24771.0
54	25459.0
55	29637.5
56	33816.0
57	37058.5
58	40301.0
59	46702.0
60	53103.0
61	57646.0
62	62189.0
63	59497.5
64	59808.0
65	62810.0
66	55742.0
67	48674.0
68	41845.0
69	35016.0
70	29179.0
71	23342.0
72	19530.0
73	15718.0
74	12857.5
75	9997.0
76	10593.5
77	11190.0
78	8259.5
79	5329.0
80	3374.0
81	1419.0
82	1047.5
83	676.0
84	508.5
85	341.0
86	193.0
87	45.0
88	30.0
89	9.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	621563.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.055498798995437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.65668064709406	5.369528108976886
2	9.88016776512882	1.5917935913173724
3	4.357898941481926	1.0531514906775339
4	2.170960655082884	0.6995268379874606
5	1.2762133013780708	0.5140267358256524
6	0.7729179149191132	0.3735743601211784
7	0.5012981825444377	0.2826744835197719
8	0.43539045336528864	0.28058298193425285
9	0.2915917715198722	0.21140254487477536
>10	6.09746355102856	14.07049647421098
>50	4.7313760734971035	27.309057971597404
>100	2.7102057120031957	38.93716324813414
>500	0.08388256440982625	4.452163336620745
>1k	0.03395246654683443	4.854857834201843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4452	0.7162588506716133	No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3187	0.5127396579268715	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2937	0.4725184735899659	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2201	0.3541073069021161	No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2151	0.346063070034735	No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1690	0.27189520611748125	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1430	0.23006517440709953	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1349	0.21703351068194213	No Hit
ACGAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1340	0.21558554804581354	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1320	0.2123678532988611	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1270	0.20432361643148	No Hit
ACGAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1231	0.19804911167492276	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTGCTCT	1224	0.1969229185134894	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1152	0.18533921742446058	No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1116	0.17954736687994619	No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1077	0.17327286212338894	No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1049	0.1687680894776555	No Hit
ACGAGCGGGGGGCTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG	989	0.1591150052367982	No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	971	0.15621907996454099	No Hit
ACGAGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	951	0.15300138521758855	No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	862	0.1386826435936502	No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	832	0.13385610147322155	No Hit
ACGAGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	787	0.12661628829257857	No Hit
ACGAGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	785	0.1262945188178833	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	767	0.1233985935456261	No Hit
ACGAGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	766	0.12323770880827847	No Hit
ACGAGCGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT	750	0.12066355301071653	No Hit
ACGAGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	746	0.12002001406132604	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGC	721	0.11599789562763549	No Hit
ACGAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	708	0.11390639404211642	No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	698	0.11229754666864018	No Hit
ACGAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	698	0.11229754666864018	No Hit
ACGAGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	678	0.10907985192168775	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACCGACTGCTCT	675	0.10859719770964488	No Hit
ACGAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	653	0.10505773348799718	No Hit
ACGAGCGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG	648	0.10425330980125909	No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	625	0.10055296084226378	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.003861233696342929	0.0	0.0	0.0	0.0
8	0.004987426857776284	0.0	0.0	0.0	0.0
9	0.006918043705947748	0.0	0.0	0.0	0.0
10	0.012066355301071653	0.0	0.0	0.0	0.0
11	0.020271476905800376	0.0	0.0	0.0	0.0
12	0.02590244271296715	0.0	0.0	0.0	0.0
13	0.03346402536830539	0.0	0.0	0.0	0.0
14	0.03861233696342929	0.0	0.0	0.0	0.0
15	0.0489089601536771	0.0	0.0	0.0	0.0
16	0.05630965807166771	0.0	0.0	0.0	0.0
17	0.0649974338884393	0.0	0.0	0.0	0.0
18	0.07416786391725376	0.0	0.0	0.0	0.0
19	0.08430360237015395	0.0	0.0	0.0	0.0
20	0.0926696087122303	0.0	0.0	0.0	0.0
21	0.10055296084226378	0.0	0.0	0.0	0.0
22	0.10891896718434012	0.0	0.0	0.0	0.0
23	0.11921559037458794	0.0	0.0	0.0	0.0
24	0.12822513566605476	0.0	0.0	0.0	0.0
25	0.13626937253343588	0.0	0.0	0.0	0.0
26	0.14560068729959794	0.0	0.0	0.0	0.0
27	0.15316226995493618	0.0	0.0	0.0	0.0
28	0.16024119839823156	0.0	0.0	0.0	0.0
29	0.16957251316439365	0.0	0.0	0.0	0.0
30	0.17938648214259859	0.0	0.0	0.0	0.0
31	0.18646541058589394	0.0	0.0	0.0	0.0
32	0.19450964745327504	0.0	0.0	0.0	0.0
33	0.20287565379535138	0.0	0.0	0.0	0.0
34	0.20963281276395151	0.0	0.0	0.0	0.0
35	0.22314713070115177	0.0	0.0	0.0	0.0
36	0.23891383496121874	0.0	0.0	0.0	0.0
37	0.25065842078759515	0.0	0.0	0.0	0.0
38	0.26015062029110486	0.0	0.0	0.0	0.0
39	0.2723778603295241	0.0	0.0	0.0	0.0
40	0.2810656361462957	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACGTA	35	1.0185613E-7	46.000004	28
TCGACGC	35	1.0185613E-7	46.000004	35
TCAACGA	35	1.0185613E-7	46.000004	13
GCATAAC	20	6.309206E-4	46.0	31
TCACGAC	25	3.415332E-5	46.0	24
CTTAACG	55	1.8189894E-12	46.0	17
CGTGGAA	20	6.309206E-4	46.0	21
ACTATCG	20	6.309206E-4	46.0	45
GGTACTC	20	6.309206E-4	46.0	8
ACTATAG	20	6.309206E-4	46.0	35
GATACGA	25	3.415332E-5	46.0	34
TCTGACG	20	6.309206E-4	46.0	43
GTCGCAA	30	1.8600131E-6	46.0	10
TCGCAAA	30	1.8600131E-6	46.0	11
CGAACGT	30	1.8600131E-6	46.0	38
ACAAAAC	20	6.309206E-4	46.0	34
CGTATTG	25	3.415332E-5	46.0	13
AATTTCG	20	6.309206E-4	46.0	31
ATTAGGT	20	6.309206E-4	46.0	27
CGTATAT	20	6.309206E-4	46.0	28
>>END_MODULE