SRR1527757_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527757_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	624757
Sequences flagged as poor quality	0
Sequence length	52
%GC	59

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4182	0.6693802550431608	No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3093	0.49507248418184985	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2693	0.4310475913034988	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2152	0.3444539236855289	No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2027	0.3244461446610442	No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1531	0.24505527749188885	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1415	0.226488058557167	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1309	0.20952146194440396	No Hit
ACGAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1276	0.20423940828194	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1223	0.19575610997555848	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTGCTCT	1217	0.19479573658238322	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1216	0.19463567435018736	No Hit
ACGAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1200	0.19207467863505331	No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1091	0.17462789532570264	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1062	0.1699860905920222	No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1039	0.16630465925151697	No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	990	0.158461609873919	No Hit
ACGAGCGGGGGGCTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG	943	0.15093868496071272	No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	909	0.14549656906605288	No Hit
ACGAGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	889	0.14229532442213533	No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	815	0.13045071923964038	No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	810	0.129650408078661	No Hit
ACGAGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	747	0.11956648745032067	No Hit
ACGAGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	727	0.11636524280640313	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	725	0.11604511834201138	No Hit
ACGAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	720	0.11524480718103197	No Hit
ACGAGCGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT	704	0.11268381146589794	No Hit
ACGAGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	700	0.11204356253711444	No Hit
ACGAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	698	0.11172343807272268	No Hit
ACGAGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	691	0.11060300244735152	No Hit
ACGAGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	683	0.10932250458978451	No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	665	0.1064413844102587	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGC	645	0.10324013976634117	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGCACACCAACTGCTCT	628	0.10051908181901122	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	35	1.0185613E-7	46.000004	17
TCGTTTC	35	1.0185613E-7	46.000004	29
CGAAAGT	35	1.0185613E-7	46.000004	15
GCGAATT	70	0.0	46.000004	28
TAACGAG	35	1.0185613E-7	46.000004	19
ATCGTTT	35	1.0185613E-7	46.000004	28
TAGCATA	35	1.0185613E-7	46.000004	30
AATTCGC	35	1.0185613E-7	46.000004	33
CTCGAAC	35	1.0185613E-7	46.000004	13
CCACGTA	55	1.8189894E-12	46.000004	28
GTGCGTT	35	1.0185613E-7	46.000004	14
TATAACA	35	1.0185613E-7	46.000004	34
TCGAACC	35	1.0185613E-7	46.000004	14
AGTAAGC	55	1.8189894E-12	46.000004	46
GATAGCC	35	1.0185613E-7	46.000004	9
TATTTCG	25	3.4153534E-5	46.0	20
ACAACGG	20	6.3092326E-4	46.0	41
ACACGAA	45	3.092282E-10	46.0	39
AATTTCG	45	3.092282E-10	46.0	31
AATCACG	30	1.8600294E-6	46.0	22

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content