##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527757_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	624757
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.165401908261934	33.0	31.0	34.0	31.0	34.0
2	32.600129330283615	34.0	31.0	34.0	31.0	34.0
3	32.73146199242265	34.0	31.0	34.0	31.0	34.0
4	36.18520960949617	37.0	37.0	37.0	35.0	37.0
5	36.13732699273478	37.0	37.0	37.0	35.0	37.0
6	35.8953705200582	37.0	37.0	37.0	35.0	37.0
7	36.464713480601255	37.0	37.0	37.0	35.0	37.0
8	36.615378459144914	37.0	37.0	37.0	35.0	37.0
9	38.66408539640212	39.0	39.0	39.0	38.0	39.0
10	37.96929686262018	39.0	38.0	39.0	37.0	39.0
11	37.6337023834867	39.0	38.0	39.0	35.0	39.0
12	36.75375385950058	39.0	35.0	39.0	33.0	39.0
13	36.5093996545857	39.0	35.0	39.0	32.0	39.0
14	37.38708329798626	40.0	36.0	40.0	33.0	40.0
15	37.422397828275635	40.0	36.0	40.0	33.0	40.0
16	37.44261368820197	40.0	35.0	40.0	33.0	40.0
17	37.36151975888225	40.0	35.0	40.0	33.0	40.0
18	37.32820280525068	40.0	35.0	40.0	33.0	40.0
19	37.226275175788345	39.0	35.0	40.0	33.0	40.0
20	37.10294402463678	39.0	35.0	40.0	32.0	40.0
21	37.0375393953169	39.0	35.0	40.0	32.0	40.0
22	36.87723867039505	39.0	35.0	40.0	32.0	40.0
23	36.803390758326834	39.0	35.0	40.0	32.0	40.0
24	36.71793513317978	38.0	35.0	40.0	32.0	40.0
25	36.628263468836685	38.0	35.0	40.0	32.0	40.0
26	36.39794512106307	38.0	35.0	40.0	32.0	40.0
27	36.23567242944057	38.0	35.0	40.0	31.0	40.0
28	36.016289533370575	37.0	35.0	40.0	31.0	40.0
29	35.8526483096628	37.0	35.0	40.0	31.0	40.0
30	35.67450384709575	37.0	34.0	40.0	31.0	40.0
31	35.477139111686625	36.0	34.0	40.0	30.0	40.0
32	35.34053720086369	36.0	34.0	40.0	30.0	40.0
33	34.8805151442881	35.0	34.0	39.0	29.0	40.0
34	34.67549463231305	35.0	33.0	39.0	28.0	40.0
35	34.59192934212822	35.0	33.0	39.0	28.0	40.0
36	34.49004813071322	35.0	33.0	39.0	28.0	40.0
37	34.514542454106156	35.0	33.0	39.0	29.0	40.0
38	34.1564480269929	35.0	33.0	38.0	27.0	40.0
39	34.19972725395634	35.0	33.0	38.0	28.0	40.0
40	34.02343631203812	35.0	33.0	38.0	27.0	40.0
41	33.71053705680769	35.0	33.0	38.0	26.0	40.0
42	33.76195224703365	35.0	33.0	37.0	27.0	40.0
43	33.75005962318149	35.0	33.0	37.0	27.0	40.0
44	33.62236517558026	35.0	33.0	37.0	27.0	40.0
45	33.54279183746641	35.0	33.0	37.0	26.0	40.0
46	33.15209113303252	35.0	33.0	36.0	25.0	40.0
47	32.93934281648705	35.0	32.0	36.0	24.0	40.0
48	32.90080623346357	35.0	32.0	36.0	24.0	39.0
49	32.84988723615742	35.0	32.0	35.0	24.0	39.0
50	32.767475994666725	35.0	32.0	35.0	24.0	39.0
51	33.16388611892304	35.0	33.0	35.0	26.0	39.0
52	32.95567716728264	35.0	33.0	35.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	4.0
15	4.0
16	15.0
17	69.0
18	163.0
19	330.0
20	579.0
21	906.0
22	1396.0
23	2021.0
24	2823.0
25	4023.0
26	5263.0
27	6940.0
28	8881.0
29	11597.0
30	14752.0
31	19406.0
32	26007.0
33	38551.0
34	74210.0
35	93430.0
36	106141.0
37	89366.0
38	94239.0
39	23640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.09372764130694	0.7130772444326354	0.14565663129824877	0.04753848296217569
8	99.6387395419339	0.2533785135660745	0.04273661599629936	0.06514532850372225
9	98.77472361254055	0.648091978161109	0.22440724953862062	0.3527771597597146
10	62.700537969162404	28.679950764857377	2.8002887522668813	5.819222513713332
11	41.43947166658396	22.596305443556457	19.21803197083026	16.74619091902932
12	34.71381673194538	14.286034410178678	25.15906184324465	25.841087014631288
13	27.254756649385282	15.338923773563163	27.78792394482975	29.618395632221805
14	26.743197755287255	16.450395913931338	29.928436175985222	26.87797015479618
15	28.21929166059764	17.505206024102172	28.6906749344145	25.584827380885688
16	34.420742784794726	18.666777643147654	24.402287609422544	22.510191962635073
17	33.16169326634195	19.766405178333336	23.064487472729397	24.007414082595314
18	31.272958926430594	21.00368623320747	22.805506781036467	24.917848059325465
19	31.579157976621307	21.44993973656958	22.02648389693913	24.94441838986998
20	31.593243453054548	22.865530118109923	21.7598202181008	23.781406210734733
21	31.423577486926916	21.85873867759785	23.79597187386456	22.921711961610676
22	31.92425214923562	18.548811778019296	25.685186400472503	23.84174967227258
23	32.37754839081435	20.077406095489927	23.57540611789864	23.969639395797085
24	31.26591618821398	19.28477792165594	22.949562790012756	26.499743100117325
25	29.647847082945848	20.46011489267027	21.423049281560672	28.46898874282321
26	26.477174325377707	21.109647430921143	23.4409538428541	28.97222440084705
27	27.190091507578146	19.229076264851773	22.82807555577609	30.752756671793996
28	28.440017478795752	22.38294248803935	21.32605156884997	27.850988464314923
29	31.09464959976439	21.77374563230184	21.083557287073216	26.04804748086056
30	29.405192738936897	19.058129800866578	23.769881730016632	27.766795730179894
31	32.79531081684559	20.090371136297794	20.63906446826526	26.475253578591357
32	34.82714079234006	19.62683091185853	22.412073814299	23.133954481502407
33	34.08669290620193	18.631403889832367	25.070867553304726	22.211035650660975
34	28.504842682835086	20.9054080226392	28.686833440841795	21.902915853683915
35	27.421061308636794	26.214032015647682	26.781292566549876	19.58361410916564
36	29.99758306029384	26.212911580022315	22.668493510276797	21.121011849407047
37	30.20758470893483	26.656283963204896	20.082848211384587	23.053283116475686
38	31.10793476503665	26.52679361735843	20.732540811867654	21.63273080573727
39	31.387563484682847	22.886658332759776	21.17415251049608	24.5516256720613
40	28.624249108053213	23.429429362136	20.285486997344567	27.66083453246622
41	26.92198726865005	24.399726613707408	21.08675853171713	27.59152758592541
42	24.629095792444105	22.43608314912838	21.98454759210381	30.950273466323708
43	25.448614421286997	20.353993632724404	24.5514656098291	29.6459263361595
44	28.009930260885437	19.34272044971085	23.50161742885634	29.14573186054738
45	28.8187247201712	22.35493159740507	22.53083999058834	26.295503691835385
46	27.513897403310406	22.635200565980053	22.08826791856674	27.762634112142802
47	26.432997149291644	22.6995455833228	24.162674447825314	26.704782819560247
48	28.55526228597679	20.875476385218573	25.14801754922314	25.421243779581502
49	28.12501500583427	20.96959297774975	24.137704739602757	26.767687276813223
50	26.397303271511962	20.97183384900049	24.58571892751902	28.045143951968527
51	26.32911676059652	20.275243014484033	24.831414453939693	28.564225770979757
52	26.39154103115291	20.55022992939655	26.270533983612825	26.787695055837705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.0
8	8.0
9	5.0
10	2.0
11	7.5
12	13.0
13	33.5
14	278.5
15	503.0
16	834.0
17	1165.0
18	900.5
19	636.0
20	705.5
21	775.0
22	832.5
23	890.0
24	843.0
25	796.0
26	852.0
27	908.0
28	1284.0
29	1660.0
30	2138.0
31	2616.0
32	3120.0
33	3624.0
34	3493.5
35	3363.0
36	4541.5
37	5720.0
38	6476.0
39	8060.0
40	8888.0
41	10059.0
42	11230.0
43	11142.0
44	11054.0
45	12888.5
46	14723.0
47	16899.5
48	19076.0
49	18034.0
50	16992.0
51	20469.0
52	23946.0
53	24707.5
54	25469.0
55	29574.5
56	33680.0
57	37087.5
58	40495.0
59	46928.0
60	53361.0
61	57764.5
62	62168.0
63	59931.0
64	60291.5
65	62889.0
66	56069.0
67	49249.0
68	42164.5
69	35080.0
70	29378.0
71	23676.0
72	19809.0
73	15942.0
74	13068.0
75	10194.0
76	10684.5
77	11175.0
78	8284.0
79	5393.0
80	3371.5
81	1350.0
82	1014.5
83	679.0
84	495.0
85	311.0
86	184.0
87	57.0
88	36.5
89	9.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	624757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.02611575380508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15995421513563	8.798300779343009
2	10.131232198472064	2.436787422950043
3	3.652141507173849	1.3176322954364657
4	1.6597013336172706	0.7983904141930382
5	0.8983948678361328	0.5402100336610874
6	0.5430297867809514	0.39183234441550874
7	0.3793222775308116	0.3193241532307761
8	0.31277450954294994	0.30091699652825016
9	0.2089599914818857	0.22616793409277525
>10	4.267042883381691	14.770382724803405
>50	3.039902041685522	25.954090950561575
>100	1.6716799318550857	35.50260341220667
>500	0.05456916975004659	4.2054750887144925
>1k	0.02129528575611574	4.437885449862907
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4182	0.6693802550431608	No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3093	0.49507248418184985	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2693	0.4310475913034988	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2152	0.3444539236855289	No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2027	0.3244461446610442	No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1531	0.24505527749188885	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1415	0.226488058557167	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1309	0.20952146194440396	No Hit
ACGAGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1276	0.20423940828194	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1223	0.19575610997555848	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGCACTGACTGCTCT	1217	0.19479573658238322	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1216	0.19463567435018736	No Hit
ACGAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1200	0.19207467863505331	No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1091	0.17462789532570264	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1062	0.1699860905920222	No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1039	0.16630465925151697	No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	990	0.158461609873919	No Hit
ACGAGCGGGGGGCTGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG	943	0.15093868496071272	No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	909	0.14549656906605288	No Hit
ACGAGCGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	889	0.14229532442213533	No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	815	0.13045071923964038	No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	810	0.129650408078661	No Hit
ACGAGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	747	0.11956648745032067	No Hit
ACGAGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	727	0.11636524280640313	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	725	0.11604511834201138	No Hit
ACGAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	720	0.11524480718103197	No Hit
ACGAGCGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT	704	0.11268381146589794	No Hit
ACGAGCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	700	0.11204356253711444	No Hit
ACGAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	698	0.11172343807272268	No Hit
ACGAGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	691	0.11060300244735152	No Hit
ACGAGCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	683	0.10932250458978451	No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	665	0.1064413844102587	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGC	645	0.10324013976634117	No Hit
ACGAGCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGCACACCAACTGCTCT	628	0.10051908181901122	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.005762240359051599	0.0	0.0	0.0	0.0
8	0.007362862681010377	0.0	0.0	0.0	0.0
9	0.00928360946736091	0.0	0.0	0.0	0.0
10	0.014885787594216632	0.0	0.0	0.0	0.0
11	0.021288276882051742	0.0	0.0	0.0	0.0
12	0.025609957151340443	0.0	0.0	0.0	0.0
13	0.03265269536795906	0.0	0.0	0.0	0.0
14	0.03617406447626837	0.0	0.0	0.0	0.0
15	0.044657362782649895	0.0	0.0	0.0	0.0
16	0.05314066108903141	0.0	0.0	0.0	0.0
17	0.06162395939541294	0.0	0.0	0.0	0.0
18	0.07010725770179446	0.0	0.0	0.0	0.0
19	0.07955092940135125	0.0	0.0	0.0	0.0
20	0.08915466333310391	0.0	0.0	0.0	0.0
21	0.09747789940728956	0.0	0.0	0.0	0.0
22	0.10275995306975352	0.0	0.0	0.0	0.0
23	0.11092312691174329	0.0	0.0	0.0	0.0
24	0.1194064252181248	0.0	0.0	0.0	0.0
25	0.12628910120254755	0.0	0.0	0.0	0.0
26	0.13621295959869198	0.0	0.0	0.0	0.0
27	0.14293557335091883	0.0	0.0	0.0	0.0
28	0.14981824933534157	0.0	0.0	0.0	0.0
29	0.15718111201635196	0.0	0.0	0.0	0.0
30	0.16438391246516645	0.0	0.0	0.0	0.0
31	0.1727071485393521	0.0	0.0	0.0	0.0
32	0.18391150479306353	0.0	0.0	0.0	0.0
33	0.188553309526744	0.0	0.0	0.0	0.0
34	0.19607623443995026	0.0	0.0	0.0	0.0
35	0.20776077739024934	0.0	0.0	0.0	0.0
36	0.22184625382348658	0.0	0.0	0.0	0.0
37	0.23177011221963098	0.0	0.0	0.0	0.0
38	0.24057353499040426	0.0	0.0	0.0	0.0
39	0.2504973933865487	0.0	0.0	0.0	0.0
40	0.261861811872456	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	35	1.0185613E-7	46.000004	17
TCGTTTC	35	1.0185613E-7	46.000004	29
CGAAAGT	35	1.0185613E-7	46.000004	15
GCGAATT	70	0.0	46.000004	28
TAACGAG	35	1.0185613E-7	46.000004	19
ATCGTTT	35	1.0185613E-7	46.000004	28
TAGCATA	35	1.0185613E-7	46.000004	30
AATTCGC	35	1.0185613E-7	46.000004	33
CTCGAAC	35	1.0185613E-7	46.000004	13
CCACGTA	55	1.8189894E-12	46.000004	28
GTGCGTT	35	1.0185613E-7	46.000004	14
TATAACA	35	1.0185613E-7	46.000004	34
TCGAACC	35	1.0185613E-7	46.000004	14
AGTAAGC	55	1.8189894E-12	46.000004	46
GATAGCC	35	1.0185613E-7	46.000004	9
TATTTCG	25	3.4153534E-5	46.0	20
ACAACGG	20	6.3092326E-4	46.0	41
ACACGAA	45	3.092282E-10	46.0	39
AATTTCG	45	3.092282E-10	46.0	31
AATCACG	30	1.8600294E-6	46.0	22
>>END_MODULE