FastQCFastQC Report
Fri 17 Jun 2016
SRR1527756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513971
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA35720.694980845222785No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC28380.5521712314508017No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT26800.5214301974235901No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC17960.34943605767640584No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16550.32200260325971697No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG16090.3130526819606554No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC13710.2667465674133365No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGA12950.25195974091923473No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12610.2453445816981892No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG11320.2202458893595164No Hit
CGTATTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA10870.2114905315669561No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8990.17491259234470427No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG8270.16090401987660782No Hit
CGTATTGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGC8200.15954207533109846No Hit
CGTATTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA7920.15409429714906092No Hit
CGTATTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA7320.14242048675898056No Hit
CGTATTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA7290.14183679623947654No Hit
CGTATTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC6670.1297738588363935No Hit
CGTATTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6470.1258825887063667No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6430.12510433468036133No Hit
CGTATTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG6340.12335326312184929No Hit
CGTATTGGGGGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGT6130.11926742948532117No Hit
CGTATTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT5960.11595984987479839No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC5950.11576528636829705No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG5930.11537615935529437No Hit
CGTATTGGGATATGTGGTTGCTGTGGGCTCCCCCTGGCCCCTGTGTCATA5600.10895556364075017No Hit
CGTATTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT5570.10837187312124615No Hit
CGTATTGGGAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTG5450.10603711104323008No Hit
CGTATTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT5170.10058933286119255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA302.5249738E-644.00000427
CACGGTA302.5249738E-644.00000427
CTCAAAC302.5249738E-644.00000422
CGCGCAT302.5249738E-644.00000439
ATATGCT1750.044.00000434
AGTATAC650.044.00000417
TTATAGC302.5249738E-644.00000413
GTTACGC302.5249738E-644.00000416
TACGCGA302.5249738E-644.00000418
ACCTAAT302.5249738E-644.00000411
AATTAGT302.5249738E-644.00000430
GTATACC650.044.00000418
TTACGCG302.5249738E-644.00000417
TATACCC650.044.00000419
TAGCTTA302.5249738E-644.00000416
TCGAAGT302.5249738E-644.00000439
AGCGTAC207.8524166E-444.011
ATTAGTT254.4393946E-544.010
TCCGCGA207.8524166E-444.041
CATATCC207.8524166E-444.029
Produced by FastQC (version 0.11.5)