##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527756_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	513971
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.990641495337286	33.0	31.0	33.0	31.0	34.0
2	32.75595510252524	34.0	31.0	34.0	31.0	34.0
3	30.76448476665026	31.0	30.0	33.0	28.0	33.0
4	35.54462605866868	35.0	35.0	37.0	35.0	37.0
5	36.16414933916505	37.0	35.0	37.0	35.0	37.0
6	36.51027198032574	37.0	37.0	37.0	35.0	37.0
7	36.41005620939703	37.0	36.0	37.0	35.0	37.0
8	36.657091547966715	37.0	37.0	37.0	35.0	37.0
9	38.753945261503084	39.0	39.0	39.0	39.0	39.0
10	38.26893346122641	39.0	39.0	39.0	37.0	39.0
11	38.070858083432725	39.0	38.0	39.0	37.0	39.0
12	37.688671150706945	39.0	37.0	39.0	35.0	39.0
13	37.577571497224554	39.0	37.0	39.0	35.0	39.0
14	38.43248354479144	40.0	38.0	40.0	35.0	40.0
15	38.42544034585609	40.0	38.0	40.0	35.0	40.0
16	38.4266797153925	40.0	38.0	40.0	35.0	40.0
17	38.40941609546064	40.0	38.0	40.0	35.0	40.0
18	38.30148199022902	40.0	38.0	40.0	35.0	40.0
19	38.26453048907429	40.0	38.0	40.0	34.0	40.0
20	38.1977465654677	40.0	38.0	40.0	34.0	40.0
21	38.11473020851371	40.0	38.0	40.0	34.0	40.0
22	38.21900457418804	40.0	38.0	40.0	34.0	40.0
23	38.14790328637219	40.0	38.0	40.0	34.0	40.0
24	38.02655791863743	40.0	38.0	40.0	34.0	40.0
25	37.98581632037605	40.0	38.0	40.0	34.0	40.0
26	37.83156831805685	40.0	37.0	40.0	34.0	40.0
27	37.68207350220148	40.0	37.0	40.0	34.0	40.0
28	37.51678791215847	40.0	37.0	40.0	33.0	40.0
29	37.6058804874205	40.0	37.0	40.0	33.0	40.0
30	37.726260820163006	40.0	37.0	40.0	34.0	40.0
31	37.75209885382638	40.0	37.0	40.0	34.0	40.0
32	37.67120129345819	40.0	37.0	40.0	34.0	40.0
33	37.592296063396574	40.0	37.0	40.0	34.0	40.0
34	37.15169922038403	40.0	36.0	40.0	33.0	40.0
35	37.12379297664654	40.0	36.0	40.0	33.0	40.0
36	37.03041416733629	40.0	35.0	40.0	33.0	40.0
37	36.86411101015427	40.0	35.0	40.0	32.0	40.0
38	36.71680503374704	40.0	35.0	40.0	32.0	40.0
39	36.52701027879005	40.0	35.0	40.0	31.0	40.0
40	36.25811183899481	39.0	35.0	40.0	31.0	40.0
41	36.12119166256462	39.0	35.0	40.0	30.0	40.0
42	36.025758262625715	39.0	35.0	40.0	30.0	40.0
43	35.83313455428419	39.0	35.0	40.0	30.0	40.0
44	35.51941841076636	39.0	35.0	40.0	28.0	40.0
45	35.426175795910666	38.0	35.0	40.0	28.0	40.0
46	35.240215887666814	38.0	35.0	40.0	27.0	40.0
47	35.15219730296067	38.0	35.0	40.0	27.0	40.0
48	35.03768695120931	37.0	35.0	40.0	27.0	40.0
49	34.863070095394484	37.0	34.0	40.0	27.0	40.0
50	34.148123143134534	36.0	33.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	18.0
17	34.0
18	79.0
19	132.0
20	281.0
21	446.0
22	678.0
23	1060.0
24	1571.0
25	2371.0
26	3185.0
27	4258.0
28	5601.0
29	6960.0
30	8109.0
31	9217.0
32	11561.0
33	15620.0
34	27413.0
35	30365.0
36	48120.0
37	80534.0
38	183541.0
39	72812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.52896175076026	0.259547717672787	0.15409429714906092	0.057396234417895174
8	99.20520807594202	0.6171554426222492	0.1317194939014069	0.04591698753431614
9	97.84715480056268	1.1798331034241232	0.4340711830044886	0.5389409130087106
10	53.45496146669754	31.987991540378736	6.050925052191661	8.506121940732065
11	32.968591613145485	26.532430818081178	21.283301976181534	19.2156755925918
12	27.86441258358935	19.912602072879597	26.31257405573466	25.910411287796393
13	24.756066003723944	19.200305075578193	28.46600294569149	27.57762597500637
14	25.001216021915635	20.980950287078453	28.477871319588072	25.53996237141784
15	24.285806008510207	20.827050553435893	28.382729764908916	26.504413673144985
16	29.113315731821444	22.08568187699306	24.325886090849483	24.475116300336012
17	29.255930782086924	22.73960982234406	21.764846654772352	26.23961274079666
18	28.522426362576876	22.636880290911353	22.46352420661866	26.37716913989311
19	28.78820011245771	22.08840576608408	22.841950226763768	26.281443894694444
20	27.849236630082242	23.45579808977549	22.133155372579385	26.56180990756288
21	30.557171513567887	21.242832766829256	24.981370544252496	23.21862517535036
22	29.14755890896568	21.152749863319137	22.628319496625295	27.07137173108989
23	28.111313673339545	23.942790546548345	22.572479770259413	25.373416009852694
24	27.126627766936267	23.28010724340478	23.7328565230334	25.86040846662555
25	26.8845907648486	23.076982942617384	23.15636485326993	26.88206143926408
26	27.755457019948597	23.155002908724423	21.321047296442796	27.768492774884184
27	27.256012498759656	22.874636895855993	22.454963412332603	27.41438719305175
28	27.81946841358754	23.896873559014033	21.546546400477848	26.737111626920584
29	29.12985362987406	23.584599131079383	22.642717196106396	24.64283004294017
30	29.059421640520572	23.482064163153176	22.18899509894527	25.269519097380982
31	29.76841105821146	23.116479334437155	20.55057581069749	26.564533796653894
32	31.146504374760443	23.43964931873588	21.82827435789179	23.585571948611886
33	31.55372579386775	22.19619394868582	21.964468812442725	24.285611445003706
34	26.93284251446093	25.723630321555106	24.061668849020666	23.281858314963298
35	28.116372324508582	26.03960145611328	23.93753733187281	21.906488887505326
36	29.358465750013135	25.146360397765633	21.037373703963844	24.45780014825739
37	29.400102340404423	25.31446326738279	21.72418288191357	23.56125151029922
38	29.36527547274068	24.785055966192644	21.997350045041454	23.85231851602522
39	28.39907309945503	23.539654961077574	22.410602932850296	25.650669006617104
40	28.50413739296575	22.850900148062827	22.78299748429386	25.86196497467756
41	26.88731465393962	23.10071969041055	23.629154174068187	26.382811481581648
42	26.94860215848754	23.24605862976705	23.23594132742898	26.56939788431643
43	27.18110554875664	21.628263073208412	24.674738458006384	26.515892920028563
44	26.82271956978117	22.126151086345338	25.315241521408794	25.735887822464694
45	26.81318595796261	21.631570652818933	24.884283354508327	26.670960034710127
46	26.467252043403228	23.33789260483568	23.593938179391444	26.60091717236965
47	27.27858186551381	24.836809858922003	22.708285097797347	25.17632317776684
48	29.49991341923961	23.010442223393927	23.117257588463165	24.372386768903304
49	27.553500100200207	23.02970401053756	24.195333977987087	25.22146191127515
50	25.347149936475017	24.901794070093448	23.89804094005304	25.8530150533785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.5
4	11.0
5	12.5
6	14.0
7	27.5
8	41.0
9	551.0
10	1061.0
11	1582.0
12	2103.0
13	2217.5
14	2332.0
15	2075.5
16	1819.0
17	1733.0
18	1647.0
19	1758.0
20	1869.0
21	2402.5
22	2936.0
23	3416.0
24	3896.0
25	4029.5
26	4163.0
27	4440.0
28	4717.0
29	5430.5
30	6144.0
31	5647.5
32	5151.0
33	5801.5
34	6452.0
35	6494.0
36	6536.0
37	8196.5
38	9857.0
39	10482.5
40	11108.0
41	11689.5
42	12271.0
43	13311.5
44	14352.0
45	16396.5
46	18441.0
47	20270.0
48	22099.0
49	23438.0
50	24777.0
51	26966.0
52	29155.0
53	31848.0
54	34541.0
55	39241.5
56	43942.0
57	43833.5
58	43725.0
59	42995.5
60	42266.0
61	43120.5
62	43975.0
63	39699.0
64	35423.0
65	29413.5
66	23404.0
67	20202.0
68	17000.0
69	14801.0
70	12602.0
71	10206.5
72	7811.0
73	8871.0
74	9931.0
75	6975.5
76	4020.0
77	2634.0
78	1248.0
79	1078.5
80	909.0
81	512.0
82	115.0
83	88.5
84	62.0
85	40.0
86	18.0
87	18.0
88	18.0
89	13.5
90	9.0
91	4.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	513971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.469631555087739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15181279791997	9.85327965974734
2	8.406760075111945	2.2647192156755924
3	3.2384804275603063	1.3086341447280099
4	1.5109056767297413	0.8140537112016047
5	0.803120034667052	0.5408865480737239
6	0.5387837642640474	0.43543312754999797
7	0.41167124079156436	0.38815419547017244
8	0.3668929654773942	0.395353045210722
9	0.27733641484905386	0.3362057392343148
>10	7.438971544128268	28.54421747530503
>50	2.7762530694785497	25.399098392710872
>100	1.03423371370793	23.247226010806056
>500	0.02888920988010978	2.523877806335377
>1k	0.01588906543406038	3.948860927951188
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	3572	0.694980845222785	No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2838	0.5521712314508017	No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2680	0.5214301974235901	No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1796	0.34943605767640584	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1655	0.32200260325971697	No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1609	0.3130526819606554	No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1371	0.2667465674133365	No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGA	1295	0.25195974091923473	No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1261	0.2453445816981892	No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1132	0.2202458893595164	No Hit
CGTATTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1087	0.2114905315669561	No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	899	0.17491259234470427	No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	827	0.16090401987660782	No Hit
CGTATTGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGC	820	0.15954207533109846	No Hit
CGTATTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	792	0.15409429714906092	No Hit
CGTATTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	732	0.14242048675898056	No Hit
CGTATTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	729	0.14183679623947654	No Hit
CGTATTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	667	0.1297738588363935	No Hit
CGTATTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	647	0.1258825887063667	No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	643	0.12510433468036133	No Hit
CGTATTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	634	0.12335326312184929	No Hit
CGTATTGGGGGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGT	613	0.11926742948532117	No Hit
CGTATTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	596	0.11595984987479839	No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	595	0.11576528636829705	No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	593	0.11537615935529437	No Hit
CGTATTGGGATATGTGGTTGCTGTGGGCTCCCCCTGGCCCCTGTGTCATA	560	0.10895556364075017	No Hit
CGTATTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	557	0.10837187312124615	No Hit
CGTATTGGGAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTG	545	0.10603711104323008	No Hit
CGTATTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	517	0.10058933286119255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.006031468701541527	0.0	0.0	0.0	0.0
8	0.03755075675475854	0.0	0.0	0.0	0.0
9	0.04416591597580408	0.0	0.0	0.0	0.0
10	0.10798274610824346	0.0	0.0	0.0	0.0
11	0.17588540987721096	0.0	0.0	0.0	0.0
12	0.21577092870998557	0.0	0.0	0.0	0.0
13	0.24456632767218384	0.0	0.0	0.0	0.0
14	0.26810851195884594	0.0	0.0	0.0	0.0
15	0.29418002183002545	0.0	0.0	0.0	0.0
16	0.3315362150782826	0.0	0.0	0.0	0.0
17	0.36655764624852377	0.0	0.0	0.0	0.0
18	0.39399110066521265	0.0	0.0	0.0	0.0
19	0.42745602378344305	0.0	0.0	0.0	0.0
20	0.4646176535251989	0.0	0.0	0.0	0.0
21	0.5021684102799574	0.0	0.0	0.0	0.0
22	0.5492527788532816	0.0	0.0	0.0	0.0
23	0.5994501635306272	0.0	0.0	0.0	0.0
24	0.6467290956104528	0.0	0.0	0.0	0.0
25	0.6949808452227849	0.0	0.0	0.0	0.0
26	0.7475129919781466	0.0	0.0	0.0	0.0
27	0.7920680349669534	0.0	0.0	0.0	0.0
28	0.8475186343198352	0.0	0.0	0.0	0.0
29	0.8803998669185615	0.0	0.0	0.0	0.0
30	0.9154212980888027	0.0	0.0	0.0	0.0
31	0.9434384430249956	0.0	0.0	0.0	0.0
32	0.978654437701738	0.0	0.0	0.0	0.0
33	1.0068661461444324	0.0	0.0	0.0	0.0
34	1.03702348965214	0.0	0.0	0.0	0.0
35	1.0741851193938958	0.0	0.0	0.0	0.0
36	1.1039533358886007	0.0	0.0	0.0	0.0
37	1.136250877967823	0.0	0.0	0.0	0.0
38	1.1632952053715093	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	30	2.5249738E-6	44.000004	27
CACGGTA	30	2.5249738E-6	44.000004	27
CTCAAAC	30	2.5249738E-6	44.000004	22
CGCGCAT	30	2.5249738E-6	44.000004	39
ATATGCT	175	0.0	44.000004	34
AGTATAC	65	0.0	44.000004	17
TTATAGC	30	2.5249738E-6	44.000004	13
GTTACGC	30	2.5249738E-6	44.000004	16
TACGCGA	30	2.5249738E-6	44.000004	18
ACCTAAT	30	2.5249738E-6	44.000004	11
AATTAGT	30	2.5249738E-6	44.000004	30
GTATACC	65	0.0	44.000004	18
TTACGCG	30	2.5249738E-6	44.000004	17
TATACCC	65	0.0	44.000004	19
TAGCTTA	30	2.5249738E-6	44.000004	16
TCGAAGT	30	2.5249738E-6	44.000004	39
AGCGTAC	20	7.8524166E-4	44.0	11
ATTAGTT	25	4.4393946E-5	44.0	10
TCCGCGA	20	7.8524166E-4	44.0	41
CATATCC	20	7.8524166E-4	44.0	29
>>END_MODULE