FastQCFastQC Report
Fri 17 Jun 2016
SRR1527755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762835
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG51200.671180530521017No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC42290.554379387416676No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG39410.5166254825748687No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT27300.35787555631296414No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT23740.3112075350501747No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC20920.2742401698925718No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAG20600.27004529157681545No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18000.23596190526129504No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17650.23137375710343655No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT16220.21262789462990034No Hit
CGTATTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG15820.20738429673520486No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT12520.16412461410396745No Hit
CGTATTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC12180.1596675558934763No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12120.15888101620927197No Hit
CGTATTGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC11960.1567835770513938No Hit
CGTATTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA11800.15468613789351562No Hit
CGTATTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA10300.13502264578840772No Hit
CGTATTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC10090.1322697568936926No Hit
CGTATTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT9520.12479762989375161No Hit
CGTATTGGGAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAA9380.12296237063060819No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC9100.11929185210432139No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8860.1161456933675041No Hit
CGTATTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG8710.11417934415699332No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT8680.11378607431489117No Hit
CGTATTGGGGGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGTCA8620.11299953463068685No Hit
CGTATTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG8260.10828029652546094No Hit
CGTATTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT8230.10788702668335878No Hit
CGTATTGGGATATGTGGTTGCTGTGGGCTCCCCCTGGCCCCTGTGTCATAAC8140.10670721715705231No Hit
CGTATTGGGTAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCA7880.10329887852550028No Hit
CGTATTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG7800.10225015894656118No Hit
CGTATTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG7640.10015271978868301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG253.4160672E-546.029
AGGTATA206.3101144E-446.046
AACGTCG206.3101144E-446.017
ATCTCGT253.4160672E-546.032
ACACGAA301.8605733E-646.013
GTCGATA206.3101144E-446.017
CGTATCT253.4160672E-546.036
CGAGTTA206.3101144E-446.027
GATAAGC253.4160672E-546.031
GATAACG301.8605733E-646.09
CTCAAAC750.046.022
CAAAACG206.3101144E-446.018
GTTTCAT301.8605733E-646.015
TCCGATA206.3101144E-446.025
ACGTTAG253.4160672E-546.024
CCGATAG206.3101144E-446.026
TAGACGG650.046.023
TAGACCT206.3101144E-446.015
TTTCGTA206.3101144E-446.043
GACGTTA253.4160672E-546.010
Produced by FastQC (version 0.11.5)