##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527755_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	762835
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81730911665039	31.0	31.0	33.0	31.0	34.0
2	32.478373435933065	33.0	31.0	34.0	31.0	34.0
3	30.506563018214948	31.0	30.0	31.0	28.0	33.0
4	35.40873845589151	35.0	35.0	37.0	35.0	37.0
5	35.951140154817224	37.0	35.0	37.0	35.0	37.0
6	36.36746347506342	37.0	36.0	37.0	35.0	37.0
7	36.20100808169526	37.0	35.0	37.0	35.0	37.0
8	36.55436103482405	37.0	37.0	37.0	35.0	37.0
9	38.691974017972434	39.0	39.0	39.0	38.0	39.0
10	38.08082612884831	39.0	38.0	39.0	37.0	39.0
11	37.78798036272588	39.0	38.0	39.0	35.0	39.0
12	37.42764949169873	39.0	37.0	39.0	35.0	39.0
13	37.331311489378436	39.0	37.0	39.0	35.0	39.0
14	38.1972615309995	40.0	38.0	40.0	34.0	40.0
15	38.21902901675985	40.0	38.0	40.0	34.0	40.0
16	38.21203930076622	40.0	38.0	40.0	34.0	40.0
17	38.18669437034221	40.0	38.0	40.0	34.0	40.0
18	38.15167369090301	40.0	38.0	40.0	34.0	40.0
19	38.12430866439007	40.0	38.0	40.0	34.0	40.0
20	38.131297069484226	40.0	38.0	40.0	34.0	40.0
21	38.05085634508118	40.0	38.0	40.0	34.0	40.0
22	38.077534460269916	40.0	38.0	40.0	34.0	40.0
23	38.025942700584004	40.0	38.0	40.0	34.0	40.0
24	37.90869716255809	40.0	38.0	40.0	34.0	40.0
25	37.873173097721	40.0	37.0	40.0	34.0	40.0
26	37.69220866897822	40.0	37.0	40.0	34.0	40.0
27	37.56170993727346	40.0	37.0	40.0	33.0	40.0
28	37.44205627691441	40.0	36.0	40.0	33.0	40.0
29	37.342299448766774	40.0	36.0	40.0	33.0	40.0
30	37.20088878984315	40.0	36.0	40.0	33.0	40.0
31	37.134739491502096	39.0	36.0	40.0	33.0	40.0
32	36.946791901263055	39.0	35.0	40.0	32.0	40.0
33	36.831074872023436	39.0	35.0	40.0	32.0	40.0
34	36.5961407119495	39.0	35.0	40.0	31.0	40.0
35	36.44214410717914	39.0	35.0	40.0	31.0	40.0
36	36.35087142042512	39.0	35.0	40.0	31.0	40.0
37	36.18534807658275	38.0	35.0	40.0	30.0	40.0
38	36.05164157386591	38.0	35.0	40.0	30.0	40.0
39	35.83629225192866	38.0	35.0	40.0	30.0	40.0
40	35.5646109578087	38.0	35.0	40.0	29.0	40.0
41	35.39837317375317	38.0	34.0	40.0	28.0	40.0
42	35.30677538392968	38.0	34.0	40.0	28.0	40.0
43	35.099539218835005	38.0	34.0	40.0	27.0	40.0
44	34.926853120268476	37.0	34.0	40.0	27.0	40.0
45	34.75810365282138	37.0	34.0	40.0	26.0	40.0
46	34.5994074734379	37.0	34.0	40.0	26.0	40.0
47	34.41593136130356	37.0	33.0	40.0	25.0	40.0
48	34.196279667293716	36.0	33.0	40.0	24.0	40.0
49	34.44876546042067	36.0	34.0	40.0	26.0	40.0
50	34.41233163134885	36.0	34.0	40.0	26.0	40.0
51	34.38100768842541	36.0	34.0	40.0	26.0	40.0
52	33.890912189398755	35.0	33.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	7.0
16	16.0
17	55.0
18	125.0
19	263.0
20	453.0
21	801.0
22	1277.0
23	2099.0
24	3159.0
25	4332.0
26	6190.0
27	8092.0
28	10158.0
29	11874.0
30	13963.0
31	16909.0
32	21423.0
33	29554.0
34	48067.0
35	54031.0
36	88539.0
37	129959.0
38	247114.0
39	64370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.64330425321334	0.24041896347178618	0.07970268799937077	0.03657409531550073
8	99.27703894026887	0.5857098848374812	0.092680592788742	0.044570582104911285
9	97.79729561438582	1.209960214200974	0.409787175470449	0.5829569959427661
10	53.54316464241939	32.05909534827322	5.967607674005519	8.430132335301867
11	33.07255173137048	26.52526431010638	21.07677282767571	19.32541113084743
12	27.911802683411224	19.65851068710796	26.422620881317716	26.0070657481631
13	24.79146866622533	19.004634029639437	28.50996611324861	27.693931190886627
14	25.122339693380614	20.69163056231033	28.61955730924774	25.566472435061314
15	24.509494189438083	20.65099267862644	28.326309096986897	26.51320403494858
16	29.17236361729601	21.740087961354686	24.333702570018417	24.75384585133089
17	29.380665543662783	22.516664809559078	21.676640426828868	26.42602921994927
18	28.581934494353302	22.417822989244073	22.550223836085127	26.4500186803175
19	28.82785923561452	21.660385273355313	23.046006017028585	26.465749474001587
20	27.78412107467539	23.18850078981693	22.446007327928058	26.581370807579617
21	30.669935176021028	20.959054054939795	25.012748497381477	23.358262271657697
22	29.23410698250605	20.903078647413924	22.589681910242714	27.273132459837317
23	28.110010683830712	23.644693806655436	22.748562926451985	25.496732583061863
24	27.12565626904901	22.986753360818525	23.880262442074628	26.007327928057837
25	26.986832014786945	22.778189254557013	23.189418419448504	27.045560311207534
26	27.901839847411303	22.6838044924525	21.461128553356886	27.953227106779316
27	27.137192184417337	22.728637254452142	22.382035433612774	27.752135127517747
28	27.814271762569888	23.640236748444945	21.637182352671285	26.90830913631388
29	29.24079256982178	23.295732366763456	22.65194963524222	24.81152542817254
30	29.23803968092707	23.274495795289937	22.168752089246034	25.318712434536955
31	29.909613481290187	22.930253593503185	20.462616424259505	26.697516500947128
32	31.26298609791108	23.11181317060701	21.96503831103712	23.660162420444788
33	31.684964638486697	22.12391932724639	21.794621379459517	24.396494654807395
34	26.868588882261562	25.38176669922067	24.175083733703882	23.574560684813886
35	28.22235476872456	25.847004922427523	23.88930764844298	22.041332660404937
36	29.515688189451193	24.945237174487275	20.99248199151848	24.546592644543054
37	29.5293215439774	25.263130296853188	21.66693977072368	23.540608388445733
38	29.453027194609582	24.40737512043889	22.035957972562876	24.103639712388656
39	28.498692377775008	23.379892112973316	22.42437748661244	25.697038022639234
40	28.631486494458176	22.745154587820434	22.85369706424063	25.769661853480763
41	26.818905792209325	22.93680809087155	23.679301552760425	26.5649845641587
42	26.88903891405088	23.093722757870314	23.352494313973533	26.66474401410528
43	27.316261052521185	21.401089357462624	24.799202973120007	26.483446616896185
44	26.909095675998085	21.908538543721775	25.369050974326036	25.813314805954107
45	26.853120268472214	21.45286988667274	24.927277851697944	26.766731993157105
46	26.37962337858121	23.0853330012388	23.653739013023785	26.8813046071562
47	27.41851121146775	24.610302358963605	22.665582989768428	25.305603439800215
48	29.580184443555947	22.685377571820904	23.288653509605616	24.445784475017536
49	27.584077814992757	22.836262101240766	24.394659395544252	25.185000688222225
50	25.4242398421677	24.791075396383228	23.911592939495435	25.87309182195363
51	26.130028118793707	24.184784389809067	23.62542358439243	26.05976390700479
52	27.215059613153564	23.32916030334214	23.64351399712913	25.812266086375168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	10.0
4	20.0
5	19.0
6	18.0
7	33.5
8	49.0
9	721.0
10	1393.0
11	2120.0
12	2847.0
13	3000.0
14	2843.0
15	2533.0
16	2289.0
17	2045.0
18	2064.0
19	2083.0
20	2871.5
21	3660.0
22	3941.5
23	4223.0
24	4344.0
25	4465.0
26	5360.0
27	6255.0
28	6840.5
29	7426.0
30	7200.5
31	6975.0
32	8248.0
33	9521.0
34	9894.5
35	10268.0
36	10744.0
37	11220.0
38	12592.0
39	14706.0
40	15448.0
41	16965.5
42	18483.0
43	20202.0
44	21921.0
45	23933.5
46	25946.0
47	29057.0
48	32168.0
49	32613.5
50	33059.0
51	37798.0
52	42537.0
53	45501.0
54	48465.0
55	52252.5
56	56040.0
57	63788.5
58	71537.0
59	66134.0
60	60731.0
61	59579.0
62	58427.0
63	57093.0
64	48784.0
65	41809.0
66	33805.5
67	25802.0
68	23044.5
69	20287.0
70	17198.5
71	14110.0
72	15150.5
73	16191.0
74	11564.0
75	6937.0
76	5017.0
77	3097.0
78	2186.5
79	1276.0
80	890.5
81	505.0
82	315.0
83	125.0
84	74.0
85	23.0
86	15.0
87	7.0
88	12.0
89	13.5
90	10.0
91	5.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	762835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.68037649032884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52135754528518	8.587571362090097
2	8.72595452402864	2.0384486815628544
3	3.6059796637561448	1.2635760026742349
4	1.8854795627483107	0.8809244463088348
5	1.0886399856344413	0.6357862447318228
6	0.6599178469619088	0.4624853343121383
7	0.43882292204439854	0.35879318594453585
8	0.3164912123184665	0.29573892126082313
9	0.2491526565060268	0.26191771484003745
>10	4.419653879823124	14.749978697883554
>50	3.3108123274449506	27.576736777940187
>100	1.7002985342641017	33.52887583815635
>500	0.05723777244057372	4.454829681385883
>1k	0.01907925748019124	4.233156580387633
>5k	0.0011223092635406612	0.671180530521017
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	5120	0.671180530521017	No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4229	0.554379387416676	No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3941	0.5166254825748687	No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2730	0.35787555631296414	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2374	0.3112075350501747	No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2092	0.2742401698925718	No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAG	2060	0.27004529157681545	No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1800	0.23596190526129504	No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1765	0.23137375710343655	No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	1622	0.21262789462990034	No Hit
CGTATTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1582	0.20738429673520486	No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1252	0.16412461410396745	No Hit
CGTATTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1218	0.1596675558934763	No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1212	0.15888101620927197	No Hit
CGTATTGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC	1196	0.1567835770513938	No Hit
CGTATTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1180	0.15468613789351562	No Hit
CGTATTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1030	0.13502264578840772	No Hit
CGTATTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	1009	0.1322697568936926	No Hit
CGTATTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	952	0.12479762989375161	No Hit
CGTATTGGGAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAA	938	0.12296237063060819	No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	910	0.11929185210432139	No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	886	0.1161456933675041	No Hit
CGTATTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	871	0.11417934415699332	No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	868	0.11378607431489117	No Hit
CGTATTGGGGGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGTCA	862	0.11299953463068685	No Hit
CGTATTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	826	0.10828029652546094	No Hit
CGTATTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	823	0.10788702668335878	No Hit
CGTATTGGGATATGTGGTTGCTGTGGGCTCCCCCTGGCCCCTGTGTCATAAC	814	0.10670721715705231	No Hit
CGTATTGGGTAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCA	788	0.10329887852550028	No Hit
CGTATTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	780	0.10225015894656118	No Hit
CGTATTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	764	0.10015271978868301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.004457058210491129	0.0	0.0	0.0	0.0
8	0.035001015947092096	0.0	0.0	0.0	0.0
9	0.038671534473378906	0.0	0.0	0.0	0.0
10	0.10159470920972426	0.0	0.0	0.0	0.0
11	0.158881016209272	0.0	0.0	0.0	0.0
12	0.1938820321563641	0.0	0.0	0.0	0.0
13	0.2173471327351262	0.0	0.0	0.0	0.0
14	0.23714171478760152	0.0	0.0	0.0	0.0
15	0.2637529741031809	0.0	0.0	0.0	0.0
16	0.29639437099766003	0.0	0.0	0.0	0.0
17	0.32025274141852433	0.0	0.0	0.0	0.0
18	0.3430623922604495	0.0	0.0	0.0	0.0
19	0.3768835986812351	0.0	0.0	0.0	0.0
20	0.4108358950493881	0.0	0.0	0.0	0.0
21	0.4441327416807042	0.0	0.0	0.0	0.0
22	0.478347217943592	0.0	0.0	0.0	0.0
23	0.523835429680075	0.0	0.0	0.0	0.0
24	0.5678816519955168	0.0	0.0	0.0	0.0
25	0.6082573557846717	0.0	0.0	0.0	0.0
26	0.6512548585211743	0.0	0.0	0.0	0.0
27	0.6857315146787969	0.0	0.0	0.0	0.0
28	0.731612996257382	0.0	0.0	0.0	0.0
29	0.7608460545203091	0.0	0.0	0.0	0.0
30	0.7911278323621753	0.0	0.0	0.0	0.0
31	0.8199676207830002	0.0	0.0	0.0	0.0
32	0.8495939488880295	0.0	0.0	0.0	0.0
33	0.8752875785720372	0.0	0.0	0.0	0.0
34	0.9050449966244338	0.0	0.0	0.0	0.0
35	0.9404392824136281	0.0	0.0	0.0	0.0
36	0.9661329120976357	0.0	0.0	0.0	0.0
37	0.9915643618869087	0.0	0.0	0.0	0.0
38	1.0099169545183428	0.0	0.0	0.0	0.0
39	1.0335131450444723	0.0	0.0	0.0	0.0
40	1.0513413778864367	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.4160672E-5	46.0	29
AGGTATA	20	6.3101144E-4	46.0	46
AACGTCG	20	6.3101144E-4	46.0	17
ATCTCGT	25	3.4160672E-5	46.0	32
ACACGAA	30	1.8605733E-6	46.0	13
GTCGATA	20	6.3101144E-4	46.0	17
CGTATCT	25	3.4160672E-5	46.0	36
CGAGTTA	20	6.3101144E-4	46.0	27
GATAAGC	25	3.4160672E-5	46.0	31
GATAACG	30	1.8605733E-6	46.0	9
CTCAAAC	75	0.0	46.0	22
CAAAACG	20	6.3101144E-4	46.0	18
GTTTCAT	30	1.8605733E-6	46.0	15
TCCGATA	20	6.3101144E-4	46.0	25
ACGTTAG	25	3.4160672E-5	46.0	24
CCGATAG	20	6.3101144E-4	46.0	26
TAGACGG	65	0.0	46.0	23
TAGACCT	20	6.3101144E-4	46.0	15
TTTCGTA	20	6.3101144E-4	46.0	43
GACGTTA	25	3.4160672E-5	46.0	10
>>END_MODULE