FastQCFastQC Report
Fri 17 Jun 2016
SRR1527754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767255
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG48190.628083231780829No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC40190.5238154199060286No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG38170.4974877974076415No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT26160.34095574483059743No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT21310.27774338388149966No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19660.25623814768232206No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAG17890.23316889430502244No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC16660.21713771822927191No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16170.21075131475194034No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT15470.20162788121289532No Hit
CGTATTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG15070.19641449061915528No Hit
CGTATTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA11670.15210067057236512No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT11630.1515793315129911No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11510.15001531433486912No Hit
CGTATTGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC11210.14610527138956408No Hit
CGTATTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11010.14349857609269406No Hit
CGTATTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC9600.1251213742497605No Hit
CGTATTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA9470.12342702230679499No Hit
CGTATTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT9150.11925630983180299No Hit
CGTATTGGGGGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGTCA8850.11534626688649798No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT8850.11534626688649798No Hit
CGTATTGGGAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAA8620.11234856729509744No Hit
CGTATTGGGATATGTGGTTGCTGTGGGCTCCCCCTGGCCCCTGTGTCATAAC8620.11234856729509744No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC8580.11182722823572346No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8450.11013287629275796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA351.0189797E-746.00000413
CTCAAAC700.046.00000422
GCGCCTA351.0189797E-746.0000049
CACGAAT351.0189797E-746.00000414
GCCGATA351.0189797E-746.00000444
CATCCGA351.0189797E-746.00000432
CGGCGTA206.3101365E-446.040
CAAACCG206.3101365E-446.024
CGAACGA206.3101365E-446.034
CGAACCT301.8605879E-646.031
CGAACAC253.416086E-546.026
TTAATCG253.416086E-546.020
CTACTAC206.3101365E-446.038
CCCTATA206.3101365E-446.021
TCCGATA301.8605879E-646.025
CCGATAG301.8605879E-646.026
TAGACGG600.046.023
GGACTAA206.3101365E-446.08
ATTACGA206.3101365E-446.032
TAAACGC206.3101365E-446.046
Produced by FastQC (version 0.11.5)