##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527754_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767255
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.782374829750214	31.0	31.0	33.0	31.0	34.0
2	32.45032094935843	33.0	31.0	34.0	31.0	34.0
3	30.48939531185851	31.0	30.0	31.0	28.0	33.0
4	35.37156877439704	35.0	35.0	37.0	35.0	37.0
5	35.94320923291474	37.0	35.0	37.0	35.0	37.0
6	36.310353142045344	37.0	36.0	37.0	35.0	37.0
7	36.14419456373696	37.0	35.0	37.0	35.0	37.0
8	36.52302298453578	37.0	37.0	37.0	35.0	37.0
9	38.647341496634105	39.0	39.0	39.0	38.0	39.0
10	37.99211214003167	39.0	38.0	39.0	37.0	39.0
11	37.760002867364825	39.0	38.0	39.0	35.0	39.0
12	37.385148353546086	39.0	37.0	39.0	35.0	39.0
13	37.15845058031554	39.0	37.0	39.0	34.0	39.0
14	38.14614046177607	40.0	38.0	40.0	34.0	40.0
15	38.17775446233651	40.0	38.0	40.0	34.0	40.0
16	38.200706414425454	40.0	38.0	40.0	34.0	40.0
17	38.22367922007677	40.0	38.0	40.0	35.0	40.0
18	38.190284846628565	40.0	38.0	40.0	34.0	40.0
19	38.11569817075158	40.0	38.0	40.0	34.0	40.0
20	38.13119106424852	40.0	38.0	40.0	34.0	40.0
21	38.09777714058559	40.0	38.0	40.0	34.0	40.0
22	38.01169363510176	40.0	38.0	40.0	34.0	40.0
23	37.99480355292569	40.0	38.0	40.0	34.0	40.0
24	37.9182579455331	40.0	38.0	40.0	34.0	40.0
25	37.85784647868049	40.0	37.0	40.0	34.0	40.0
26	37.63842659871881	40.0	37.0	40.0	33.0	40.0
27	37.50700223524122	40.0	37.0	40.0	33.0	40.0
28	37.41392496627588	40.0	36.0	40.0	33.0	40.0
29	37.30563763025331	40.0	36.0	40.0	33.0	40.0
30	37.14955653596262	39.0	36.0	40.0	33.0	40.0
31	37.10324859401373	39.0	36.0	40.0	32.0	40.0
32	36.92474601012701	39.0	35.0	40.0	32.0	40.0
33	36.65937269877681	39.0	35.0	40.0	31.0	40.0
34	36.514121120096966	39.0	35.0	40.0	31.0	40.0
35	36.43802256094779	39.0	35.0	40.0	31.0	40.0
36	36.20522251402728	39.0	35.0	40.0	30.0	40.0
37	36.09747476393116	38.0	35.0	40.0	30.0	40.0
38	35.67696267864009	38.0	35.0	40.0	29.0	40.0
39	35.650004235879855	38.0	35.0	40.0	29.0	40.0
40	35.461506278877295	38.0	35.0	40.0	28.0	40.0
41	35.10899375044803	38.0	34.0	40.0	27.0	40.0
42	35.14924894591759	38.0	34.0	40.0	27.0	40.0
43	35.05930362135144	38.0	34.0	40.0	27.0	40.0
44	34.88067070269989	37.0	34.0	40.0	27.0	40.0
45	34.70816091130067	37.0	34.0	40.0	26.0	40.0
46	34.36799369179738	37.0	33.0	40.0	24.0	40.0
47	34.10248483229174	36.0	33.0	40.0	23.0	40.0
48	34.05473278114838	36.0	33.0	40.0	23.0	40.0
49	33.918806654893096	36.0	33.0	39.0	24.0	40.0
50	33.61985128803332	35.0	33.0	39.0	23.0	40.0
51	33.94830401887248	36.0	33.0	39.0	24.0	40.0
52	33.66114785827398	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	3.0
16	10.0
17	52.0
18	145.0
19	275.0
20	465.0
21	830.0
22	1297.0
23	2184.0
24	3266.0
25	4472.0
26	6274.0
27	8451.0
28	10659.0
29	12652.0
30	14464.0
31	17539.0
32	22569.0
33	31603.0
34	49667.0
35	57089.0
36	93353.0
37	136489.0
38	239531.0
39	53913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.6212471733648	0.2540224566799825	0.08328391473499684	0.041446455220233165
8	99.26777929110922	0.5889828023277789	0.09475337404122489	0.0484845325217822
9	97.75146463691993	1.209767287277372	0.43479677551791773	0.6039713002847814
10	53.42799981753132	32.16257958566578	5.928146444141778	8.48127415266111
11	33.055372724843764	26.478289486546192	21.154114342689198	19.312223445920846
12	27.987305393904244	19.708962470104463	26.426546584903328	25.877185551087965
13	25.09530729679181	18.90088692807476	28.52545763794306	27.478348137190373
14	25.01971313318258	20.673309395181523	28.65657441137562	25.65040306026028
15	24.482342897732824	20.621566493538655	28.439436693146348	26.456653915582173
16	29.1332086464083	21.724850277938888	24.41495982430874	24.72698125134408
17	29.482636150953724	22.482616600738996	21.734495050537305	26.300252197769975
18	28.57654886576171	22.41823122690631	22.495910746753033	26.509309160578947
19	28.840737434099484	21.697349642556908	23.071599403066777	26.39031352027683
20	27.91822796853719	23.14211051084711	22.418491896435995	26.52116962417971
21	30.673765566858474	20.937106959224767	25.028575897191935	23.360551576724816
22	29.33392418426729	20.870245224860053	22.645795726323062	27.150034864549593
23	28.04882340291038	23.667750617460946	22.77691249975562	25.506513479873057
24	27.104939035913745	23.027155248255145	23.84878560582857	26.019120110002543
25	27.039510983962305	22.735335709770546	23.157881017393176	27.067272288873973
26	27.959935093287108	22.673557031234726	21.447106894057384	27.91940098142078
27	27.139477748597272	22.61634006946843	22.605261614456733	27.638920567477566
28	27.873523144195865	23.542629243211188	21.65095046627262	26.93289714632032
29	29.251031273826822	23.236081876299274	22.78316856846811	24.72971828140579
30	29.142071410417657	23.239470580185206	22.258962144267553	25.359495865129585
31	29.96148607698875	22.8796162944523	20.56708656183407	26.591811066724887
32	31.238506102925363	23.06390965194101	21.949286742999394	23.748297502134232
33	31.68333865533623	21.983564786153234	21.960756202305625	24.372340356204912
34	26.913607601123484	25.414106131599013	24.088992577435143	23.58329368984236
35	28.256316348541226	25.874578855791096	23.895054447348013	21.97405034831966
36	29.81081908882966	24.816130230497034	21.051931887051893	24.321118793621416
37	29.491629249727925	25.28116467145864	21.709210106157666	23.51799597265577
38	29.423203498185092	24.545294589152235	21.983173781858703	24.04832813080397
39	28.503431062684502	23.369023336439646	22.43178604245003	25.695759558425816
40	28.60261581873041	22.71904386416511	22.86358511837655	25.814755198727934
41	27.06635994552007	23.04123140285824	23.575212934422062	26.317195717199628
42	26.818984561847103	23.173781858704082	23.376582752800566	26.630650826648246
43	27.322207088907856	21.264638223276485	24.77103440186118	26.642120285954473
44	26.93094212484767	21.897283171826835	25.38269545327173	25.789079250053764
45	26.87802621032121	21.45245061941597	24.96549387100768	26.70402929925514
46	26.507875478165673	23.055046887931653	23.6897771927195	26.74730044118318
47	27.622498387107285	24.62701448670911	22.680595108536274	25.069892017647327
48	29.48850121537168	22.715655160279177	23.26227916403282	24.533564460316324
49	27.64947768342989	22.860717753549995	24.31629640732221	25.17350815569791
50	25.530364741839414	24.675890023525426	23.90873959765658	25.88500563697858
51	26.12729796482265	24.111410156988224	23.68417279783123	26.077119080357896
52	27.25156564636268	23.347387765475624	23.566089500883017	25.834957087278674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	10.0
4	20.0
5	15.0
6	10.0
7	30.0
8	50.0
9	696.0
10	1342.0
11	2027.5
12	2713.0
13	2937.5
14	2920.5
15	2679.0
16	2377.5
17	2076.0
18	2078.0
19	2080.0
20	2791.5
21	3503.0
22	3884.5
23	4266.0
24	4344.5
25	4423.0
26	5427.0
27	6431.0
28	6927.0
29	7423.0
30	7324.5
31	7226.0
32	8340.0
33	9454.0
34	9853.5
35	10253.0
36	10740.5
37	11228.0
38	12705.5
39	14867.0
40	15551.0
41	16996.0
42	18441.0
43	20349.0
44	22257.0
45	24301.0
46	26345.0
47	29234.0
48	32123.0
49	32741.5
50	33360.0
51	37897.0
52	42434.0
53	45825.0
54	49216.0
55	53346.0
56	57476.0
57	64788.0
58	72100.0
59	66437.0
60	60774.0
61	59429.5
62	58085.0
63	56962.5
64	48785.5
65	41731.0
66	33811.5
67	25892.0
68	23218.5
69	20545.0
70	17539.5
71	14534.0
72	15224.0
73	15914.0
74	11469.5
75	7025.0
76	5067.0
77	3109.0
78	2205.5
79	1302.0
80	906.5
81	511.0
82	313.5
83	116.0
84	65.0
85	14.0
86	12.5
87	11.0
88	11.5
89	13.5
90	15.0
91	7.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	767255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.0278796488386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0001255624385	12.501766251090638
2	8.497891594690778	2.7240636749716183
3	3.0439490726978082	1.46364148183183
4	1.4932770383903347	0.957362586147777
5	0.8322412270951811	0.6669531113341659
6	0.5081151735408244	0.4886405309556644
7	0.35352781309200526	0.3966410868529038
8	0.24754217373543927	0.3174060934914813
9	0.181205914419532	0.26139119291766055
>10	3.3595818435168456	15.35137894673923
>50	2.3063249509354735	26.165245800348767
>100	1.124444644616032	30.26490242893138
>500	0.03882974312343133	4.136349845505627
>1k	0.012943247707810444	4.304256968881271
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTATTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	4819	0.628083231780829	No Hit
CGTATTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4019	0.5238154199060286	No Hit
CGTATTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3817	0.4974877974076415	No Hit
CGTATTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2616	0.34095574483059743	No Hit
CGTATTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2131	0.27774338388149966	No Hit
CGTATTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1966	0.25623814768232206	No Hit
CGTATTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGTATTGGGAAG	1789	0.23316889430502244	No Hit
CGTATTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1666	0.21713771822927191	No Hit
CGTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1617	0.21075131475194034	No Hit
CGTATTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	1547	0.20162788121289532	No Hit
CGTATTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1507	0.19641449061915528	No Hit
CGTATTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1167	0.15210067057236512	No Hit
CGTATTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1163	0.1515793315129911	No Hit
CGTATTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1151	0.15001531433486912	No Hit
CGTATTGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC	1121	0.14610527138956408	No Hit
CGTATTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1101	0.14349857609269406	No Hit
CGTATTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	960	0.1251213742497605	No Hit
CGTATTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	947	0.12342702230679499	No Hit
CGTATTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	915	0.11925630983180299	No Hit
CGTATTGGGGGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGGTCA	885	0.11534626688649798	No Hit
CGTATTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	885	0.11534626688649798	No Hit
CGTATTGGGAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAA	862	0.11234856729509744	No Hit
CGTATTGGGATATGTGGTTGCTGTGGGCTCCCCCTGGCCCCTGTGTCATAAC	862	0.11234856729509744	No Hit
CGTATTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	858	0.11182722823572346	No Hit
CGTATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	845	0.11013287629275796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.003649373415618015	0.0	0.0	0.0	0.0
8	0.030759004503066126	0.0	0.0	0.0	0.0
9	0.035060051742901645	0.0	0.0	0.0	0.0
10	0.10218245563730442	0.0	0.0	0.0	0.0
11	0.15770506546063565	0.0	0.0	0.0	0.0
12	0.19472013867618979	0.0	0.0	0.0	0.0
13	0.2181803963480199	0.0	0.0	0.0	0.0
14	0.24229232784406748	0.0	0.0	0.0	0.0
15	0.2657525855158976	0.0	0.0	0.0	0.0
16	0.2967722595486507	0.0	0.0	0.0	0.0
17	0.3228392125173508	0.0	0.0	0.0	0.0
18	0.3472118135430854	0.0	0.0	0.0	0.0
19	0.38070784810786507	0.0	0.0	0.0	0.0
20	0.4147252217320187	0.0	0.0	0.0	0.0
21	0.45017627776945085	0.0	0.0	0.0	0.0
22	0.49110139393031	0.0	0.0	0.0	0.0
23	0.5395859264520922	0.0	0.0	0.0	0.0
24	0.5854637636770044	0.0	0.0	0.0	0.0
25	0.6196114720660015	0.0	0.0	0.0	0.0
26	0.6585815667542082	0.0	0.0	0.0	0.0
27	0.6950753009103884	0.0	0.0	0.0	0.0
28	0.7436901681970141	0.0	0.0	0.0	0.0
29	0.7711908035789926	0.0	0.0	0.0	0.0
30	0.8011677994929978	0.0	0.0	0.0	0.0
31	0.8252797309890454	0.0	0.0	0.0	0.0
32	0.8529107011358674	0.0	0.0	0.0	0.0
33	0.8787169845748806	0.0	0.0	0.0	0.0
34	0.9043929332490502	0.0	0.0	0.0	0.0
35	0.9361946158708643	0.0	0.0	0.0	0.0
36	0.9668232856090869	0.0	0.0	0.0	0.0
37	0.9922385647535695	0.0	0.0	0.0	0.0
38	1.012440453304312	0.0	0.0	0.0	0.0
39	1.0336850199738028	0.0	0.0	0.0	0.0
40	1.0531048999354844	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAA	35	1.0189797E-7	46.000004	13
CTCAAAC	70	0.0	46.000004	22
GCGCCTA	35	1.0189797E-7	46.000004	9
CACGAAT	35	1.0189797E-7	46.000004	14
GCCGATA	35	1.0189797E-7	46.000004	44
CATCCGA	35	1.0189797E-7	46.000004	32
CGGCGTA	20	6.3101365E-4	46.0	40
CAAACCG	20	6.3101365E-4	46.0	24
CGAACGA	20	6.3101365E-4	46.0	34
CGAACCT	30	1.8605879E-6	46.0	31
CGAACAC	25	3.416086E-5	46.0	26
TTAATCG	25	3.416086E-5	46.0	20
CTACTAC	20	6.3101365E-4	46.0	38
CCCTATA	20	6.3101365E-4	46.0	21
TCCGATA	30	1.8605879E-6	46.0	25
CCGATAG	30	1.8605879E-6	46.0	26
TAGACGG	60	0.0	46.0	23
GGACTAA	20	6.3101365E-4	46.0	8
ATTACGA	20	6.3101365E-4	46.0	32
TAAACGC	20	6.3101365E-4	46.0	46
>>END_MODULE