FastQCFastQC Report
Fri 17 Jun 2016
SRR1527750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842236
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC76740.9111460445765794No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC52950.6286836468638244No Hit
TCAAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA30800.36569322612664384No Hit
TCAAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT30750.36509956829202267No Hit
TCAAAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA27150.32235620419929806No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC25400.3015781799875569No Hit
TCAAAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA25380.30134071685370845No Hit
TCAAAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA21610.2565789161232719No Hit
TCAAAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC17480.20754277898356277No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG16360.19424484348804846No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16100.19115782274801837No Hit
TCAAAGGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT15600.18522124440180662No Hit
TCAAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG15210.18059071329176143No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC14330.1701423354024288No Hit
TCAAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG13730.16301844138697466No Hit
TCAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG12930.15351991603303586No Hit
TCAAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC12110.1437839275452486No Hit
TCAAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA11700.13891593330135496No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11240.13345428122284014No Hit
TCAAAGGGGATTGACAGTCTTGCATTTTTGTTTTGTCTCGTGATGGTGGT11030.1309609183174312No Hit
TCAAAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAG10960.13012979734896157No Hit
TCAAAGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT10950.13001106578203733No Hit
TCAAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG10790.1281113607112496No Hit
TCAAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG10440.12395575586890135No Hit
TCAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC10140.1203938088611743No Hit
TCAAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC10130.12027507729425006No Hit
TCAAAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC10120.12015634572732584No Hit
TCAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC9670.11481342521573526No Hit
TCAAAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG9300.11042035723953857No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9240.10970796783799316No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT8800.10448377889332681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTA302.5269073E-644.00000429
TAACGTC302.5269073E-644.00000423
AACGTAC207.8552833E-444.010
GATACGA207.8552833E-444.036
CATGCGG254.4418237E-544.029
CGATTCT454.802132E-1044.021
CCTAATA254.4418237E-544.012
CGTTATC207.8552833E-444.042
AACGGTT254.4418237E-544.032
CTAGTTG207.8552833E-444.033
AACATAA254.4418237E-544.022
ACGGAAC207.8552833E-444.042
CATACTC254.4418237E-544.042
GCGATAA207.8552833E-444.013
CATAAGG254.4418237E-544.025
GTATACG254.4418237E-544.043
TTACGCA207.8552833E-444.019
ATCGGAC254.4418237E-544.037
ATACGAT207.8552833E-444.016
TCGACGC207.8552833E-444.014
Produced by FastQC (version 0.11.5)