##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527750_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	842236
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.643368367061015	34.0	31.0	34.0	31.0	34.0
2	32.93498496858363	34.0	31.0	34.0	31.0	34.0
3	33.10581238512721	34.0	33.0	34.0	31.0	34.0
4	36.571204508000136	37.0	37.0	37.0	35.0	37.0
5	36.506365199302806	37.0	37.0	37.0	35.0	37.0
6	36.39208844076957	37.0	37.0	37.0	35.0	37.0
7	36.6335445172137	37.0	37.0	37.0	35.0	37.0
8	36.692741701850785	37.0	37.0	37.0	35.0	37.0
9	38.72217763192264	39.0	39.0	39.0	39.0	39.0
10	38.23116323690747	39.0	39.0	39.0	37.0	39.0
11	37.03800597457245	39.0	35.0	39.0	35.0	39.0
12	36.693558575031226	39.0	35.0	39.0	33.0	39.0
13	36.67309875141884	39.0	35.0	39.0	33.0	39.0
14	37.29356617385151	40.0	35.0	40.0	33.0	40.0
15	37.41904525572405	40.0	35.0	40.0	33.0	40.0
16	37.52750535479367	40.0	35.0	40.0	33.0	40.0
17	37.5319316676086	40.0	35.0	40.0	33.0	40.0
18	37.467143413485054	40.0	35.0	40.0	33.0	40.0
19	37.429145750122295	40.0	35.0	40.0	33.0	40.0
20	37.317593881049966	40.0	35.0	40.0	33.0	40.0
21	37.26048399735941	40.0	35.0	40.0	33.0	40.0
22	37.33131687555507	40.0	35.0	40.0	33.0	40.0
23	37.31268908002033	40.0	35.0	40.0	33.0	40.0
24	37.19367137001981	39.0	35.0	40.0	33.0	40.0
25	37.134296088032336	39.0	35.0	40.0	33.0	40.0
26	36.98884279465613	39.0	35.0	40.0	33.0	40.0
27	36.89079189205876	39.0	35.0	40.0	33.0	40.0
28	36.74643211641393	39.0	35.0	40.0	33.0	40.0
29	36.75681994120413	39.0	35.0	40.0	33.0	40.0
30	36.802150466140134	39.0	35.0	40.0	33.0	40.0
31	36.815806970967756	39.0	35.0	40.0	33.0	40.0
32	36.79053020768526	39.0	35.0	40.0	33.0	40.0
33	36.69834226986261	39.0	35.0	40.0	33.0	40.0
34	36.3112773616896	39.0	35.0	40.0	31.0	40.0
35	36.29376089362127	39.0	35.0	40.0	32.0	40.0
36	36.18710670168456	38.0	35.0	40.0	31.0	40.0
37	36.02310397560779	38.0	35.0	40.0	31.0	40.0
38	35.85824163298648	38.0	35.0	40.0	31.0	40.0
39	35.68781077987642	38.0	35.0	40.0	30.0	40.0
40	35.45030490266386	37.0	35.0	40.0	30.0	40.0
41	35.25233426260573	37.0	35.0	40.0	29.0	40.0
42	35.17366747562441	37.0	35.0	40.0	29.0	40.0
43	35.090028210620304	36.0	35.0	40.0	29.0	40.0
44	34.7619550814736	36.0	35.0	40.0	27.0	40.0
45	34.69872577282377	35.0	34.0	40.0	27.0	40.0
46	34.58804301882133	35.0	34.0	40.0	27.0	40.0
47	34.44796232884845	35.0	34.0	40.0	26.0	40.0
48	34.34298581395238	35.0	34.0	40.0	26.0	40.0
49	34.26256773635893	35.0	34.0	39.0	26.0	40.0
50	33.72101050061978	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	2.0
14	3.0
15	17.0
16	33.0
17	129.0
18	245.0
19	422.0
20	753.0
21	1170.0
22	1655.0
23	2570.0
24	3483.0
25	4704.0
26	6300.0
27	8090.0
28	10572.0
29	13309.0
30	15884.0
31	19279.0
32	23643.0
33	34973.0
34	86021.0
35	90272.0
36	63598.0
37	108131.0
38	207857.0
39	139118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.88178610270757	0.8489307035082803	0.21122345755821406	0.058059736225950925
8	99.06000218466083	0.7515708186304076	0.11505088834958373	0.07337610835917724
9	96.98053752154978	1.432733818074744	0.7938392564554354	0.7928894039200415
10	59.27839702886127	28.147811302295317	5.431494260516055	7.142297408327357
11	38.30019139528588	26.2179484135088	18.73512887124274	16.746731319962578
12	33.003576194795755	16.66528146505255	25.640556803556247	24.690585536595442
13	26.18399118536847	17.74882574480312	28.091057613305537	27.976125456522876
14	24.259708680227394	18.821921646664354	31.194938235838887	25.72343143726936
15	25.32330605673469	20.61417464938568	28.702525182965346	25.35999411091428
16	29.352105585607834	21.62647998898171	24.374165910742356	24.647248514668096
17	31.565855650910198	21.16057732037101	22.359409951605013	24.914157077113778
18	29.996699262439506	20.88155813809906	23.61190925108877	25.509833348372663
19	30.4165340830836	22.89227722396098	21.52840771470229	25.162780978253124
20	29.989575368424052	23.237786083710503	22.854876780379847	23.917761767485597
21	28.71392341339007	21.616506537360074	25.15173894252917	24.51783110672068
22	29.31589245769594	21.178268323842723	24.515456475382198	24.990382743079138
23	28.040121771095038	22.531808186779003	23.772196866436484	25.655873175689475
24	28.14413062372067	23.54043284780038	22.137619384590543	26.17781714388841
25	30.165060624338068	23.58008919115307	19.70374099421065	26.551109190298206
26	27.232034726608696	22.855114243513693	22.641397423050073	27.27145360682754
27	27.364657886863064	21.46654856833477	23.5371083639265	27.63168518087567
28	28.628080490503848	22.04109062068114	21.35672186892985	27.974107019885164
29	31.18923912062652	22.270954934246458	20.9998147787556	25.539991166371422
30	28.611576802701382	22.178937969880174	22.22726171761834	26.9822235098001
31	30.075180828176425	21.851476308303138	22.375201250005937	25.6981416135145
32	31.662503146386523	21.616625268927	22.5126924045042	24.208179180182277
33	33.0748151349503	21.986474099895993	21.22920416605322	23.70950659910049
34	28.856163830565308	23.283972663244032	25.505440280396467	22.354423225794196
35	28.714873265925466	26.077370238270507	24.058933600558515	21.148822895245516
36	29.380482430102727	25.864484538775358	22.536319986322123	22.218713044799795
37	30.114480976828347	25.467683642114565	20.149340564877303	24.26849481617979
38	30.62336447266562	24.512844380909865	21.017624513794235	23.846166632630283
39	30.594750165036878	22.24329047915311	21.57506922050352	25.586890135306493
40	29.852321677059635	23.191480772610053	21.996685014651476	24.959512535678837
41	27.930057608556268	23.7080818202974	22.859388579922967	25.502471991223363
42	27.52767632825004	22.36012234100656	22.392061132509177	27.720140198234223
43	26.664141642010076	22.345993284542576	24.842918136959238	26.146946936488106
44	27.967576783704327	20.97072554485916	24.379983757521646	26.681713913914866
45	28.66286883961265	21.980181326849006	23.28515997891327	26.07178985462507
46	26.772543562611904	25.162899709820053	22.014019823422416	26.050536904145634
47	26.306997088701976	23.491040515959895	24.986939527638334	25.215022867699787
48	28.061730916275245	23.593387126648587	24.161636405947977	24.183245551128188
49	28.73351412193257	23.609534619750285	22.637479281341573	25.019471976975577
50	25.643050166461656	23.89650881700616	23.451384172607202	27.009056843924984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	7.0
4	14.0
5	32.5
6	51.0
7	157.0
8	263.0
9	813.0
10	1363.0
11	1800.0
12	2237.0
13	2555.0
14	2873.0
15	2375.5
16	1878.0
17	2169.0
18	2460.0
19	2609.5
20	2759.0
21	2743.0
22	2727.0
23	3174.5
24	3622.0
25	3917.0
26	4212.0
27	5185.5
28	6159.0
29	7037.0
30	7915.0
31	8438.5
32	8962.0
33	11285.5
34	13609.0
35	13224.5
36	12840.0
37	13947.5
38	15055.0
39	16258.0
40	17461.0
41	19747.5
42	22034.0
43	22656.0
44	23278.0
45	26080.0
46	28882.0
47	30975.0
48	33068.0
49	35230.5
50	37393.0
51	40200.0
52	43007.0
53	48219.5
54	53432.0
55	60338.5
56	67245.0
57	76822.5
58	86400.0
59	84671.5
60	82943.0
61	79410.0
62	75877.0
63	67553.5
64	59230.0
65	49451.5
66	39673.0
67	33958.0
68	28243.0
69	24191.0
70	20139.0
71	17322.0
72	14505.0
73	13282.0
74	12059.0
75	8540.5
76	5022.0
77	3375.0
78	1728.0
79	1301.0
80	874.0
81	729.5
82	585.0
83	344.0
84	103.0
85	72.5
86	42.0
87	25.0
88	8.0
89	7.0
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	842236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.87359372124364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92699999999999	8.540319755878913
2	8.793	2.0880901918179062
3	3.376	1.2025575720875559
4	1.6070000000000002	0.7632346044015412
5	0.923	0.547966350235394
6	0.604	0.4302990364578695
7	0.45199999999999996	0.3756805053401488
8	0.366	0.3476588241580138
9	0.347	0.37081233191443885
>10	7.146	24.145902719646642
>50	3.071	25.063137834612714
>100	1.314	26.19849086623803
>500	0.047	3.607553980325455
>1k	0.025	4.77840905717729
>5k	0.002	1.5398863697080876
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	7674	0.9111460445765794	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5295	0.6286836468638244	No Hit
TCAAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3080	0.36569322612664384	No Hit
TCAAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	3075	0.36509956829202267	No Hit
TCAAAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	2715	0.32235620419929806	No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2540	0.3015781799875569	No Hit
TCAAAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	2538	0.30134071685370845	No Hit
TCAAAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	2161	0.2565789161232719	No Hit
TCAAAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1748	0.20754277898356277	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1636	0.19424484348804846	No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1610	0.19115782274801837	No Hit
TCAAAGGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1560	0.18522124440180662	No Hit
TCAAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1521	0.18059071329176143	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC	1433	0.1701423354024288	No Hit
TCAAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1373	0.16301844138697466	No Hit
TCAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1293	0.15351991603303586	No Hit
TCAAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	1211	0.1437839275452486	No Hit
TCAAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1170	0.13891593330135496	No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1124	0.13345428122284014	No Hit
TCAAAGGGGATTGACAGTCTTGCATTTTTGTTTTGTCTCGTGATGGTGGT	1103	0.1309609183174312	No Hit
TCAAAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAG	1096	0.13012979734896157	No Hit
TCAAAGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1095	0.13001106578203733	No Hit
TCAAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1079	0.1281113607112496	No Hit
TCAAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1044	0.12395575586890135	No Hit
TCAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	1014	0.1203938088611743	No Hit
TCAAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC	1013	0.12027507729425006	No Hit
TCAAAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	1012	0.12015634572732584	No Hit
TCAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	967	0.11481342521573526	No Hit
TCAAAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	930	0.11042035723953857	No Hit
TCAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	924	0.10970796783799316	No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	880	0.10448377889332681	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.04262463252580037	0.0	0.0	0.0	0.0
6	0.04262463252580037	0.0	0.0	0.0	0.0
7	0.08156858647694945	0.0	0.0	0.0	0.0
8	0.16218732041850503	0.0	0.0	0.0	0.0
9	0.23449484467536416	0.0	0.0	0.0	0.0
10	0.31072051064072304	0.0	0.0	0.0	0.0
11	0.3673554680635831	0.0	0.0	0.0	0.0
12	0.4007190383692932	0.0	0.0	0.0	0.0
13	0.4193598943763981	0.0	0.0	0.0	0.0
14	0.4465494232020479	0.0	0.0	0.0	0.0
15	0.471364320689213	0.0	0.0	0.0	0.0
16	0.49499190250713576	0.0	0.0	0.0	0.0
17	0.51838202119121	0.0	0.0	0.0	0.0
18	0.5408222873398905	0.0	0.0	0.0	0.0
19	0.5682492792993887	0.0	0.0	0.0	0.0
20	0.5971010500619779	0.0	0.0	0.0	0.0
21	0.623459457919158	0.0	0.0	0.0	0.0
22	0.6467308450363081	0.0	0.0	0.0	0.0
23	0.6781947102712303	0.0	0.0	0.0	0.0
24	0.7081150651361376	0.0	0.0	0.0	0.0
25	0.7385103462687418	0.0	0.0	0.0	0.0
26	0.7692618221021187	0.0	0.0	0.0	0.0
27	0.7969262771954654	0.0	0.0	0.0	0.0
28	0.829102531831933	0.0	0.0	0.0	0.0
29	0.8547485502875679	0.0	0.0	0.0	0.0
30	0.8802758371762783	0.0	0.0	0.0	0.0
31	0.9035472242934285	0.0	0.0	0.0	0.0
32	0.9257500273082604	0.0	0.0	0.0	0.0
33	0.9440346886145926	0.0	0.0	0.0	0.0
34	0.9641003234247884	0.0	0.0	0.0	0.0
35	0.9903399997150443	0.0	0.0	0.0	0.0
36	1.013967581532967	0.0	0.0	0.0	0.0
37	1.0333208269416174	0.0	0.0	0.0	0.0
38	1.0545737774210553	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGTA	30	2.5269073E-6	44.000004	29
TAACGTC	30	2.5269073E-6	44.000004	23
AACGTAC	20	7.8552833E-4	44.0	10
GATACGA	20	7.8552833E-4	44.0	36
CATGCGG	25	4.4418237E-5	44.0	29
CGATTCT	45	4.802132E-10	44.0	21
CCTAATA	25	4.4418237E-5	44.0	12
CGTTATC	20	7.8552833E-4	44.0	42
AACGGTT	25	4.4418237E-5	44.0	32
CTAGTTG	20	7.8552833E-4	44.0	33
AACATAA	25	4.4418237E-5	44.0	22
ACGGAAC	20	7.8552833E-4	44.0	42
CATACTC	25	4.4418237E-5	44.0	42
GCGATAA	20	7.8552833E-4	44.0	13
CATAAGG	25	4.4418237E-5	44.0	25
GTATACG	25	4.4418237E-5	44.0	43
TTACGCA	20	7.8552833E-4	44.0	19
ATCGGAC	25	4.4418237E-5	44.0	37
ATACGAT	20	7.8552833E-4	44.0	16
TCGACGC	20	7.8552833E-4	44.0	14
>>END_MODULE