FastQCFastQC Report
Fri 17 Jun 2016
SRR1527749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255636
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA112720.8977123943563262No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC74960.596988299156762No Hit
TCAAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT46470.37009133220137047No Hit
TCAAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC45110.35926016775562347No Hit
TCAAAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC38540.30693608657286026No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT38380.30566183193218416No Hit
TCAAAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG37610.2995294814739303No Hit
TCAAAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA31900.2540545189848013No Hit
TCAAAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG24870.1980669557100943No Hit
TCAAAGGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23700.1887489686501502No Hit
TCAAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG22910.18245733636181186No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT21830.17385611753724806No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC21620.17218365832136065No Hit
TCAAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG20810.1657327442029378No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT20200.1608746483853601No Hit
TCAAAGGGGATTGACAGTCTTGCATTTTTGTTTTGTCTCGTGATGGTGGTGG18210.14502610629195087No Hit
TCAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC17680.1408051377947112No Hit
TCAAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT17360.13825662851335896No Hit
TCAAAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA17000.1353895555718377No Hit
TCAAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT16830.1340356600161193No Hit
TCAAAGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC16610.13228355988518964No Hit
TCAAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA16440.13092966432947128No Hit
TCAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT15730.125275159361471No Hit
TCAAAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT15470.1232044955703723No Hit
TCAAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT15310.12193024092969618No Hit
TCAAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG15150.12065598628902008No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14820.11802783609262557No Hit
TCAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG14700.11707214511211848No Hit
TCAAAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG14090.11221404929454078No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG13360.10640026249645598No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC12610.10042719386828666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG700.046.00000438
ATAGCCG700.046.00000415
ACGTGCG551.8189894E-1246.00000431
CGATAAT351.0196709E-746.00000414
ATTTACG206.311679E-446.044
GTCGCAA206.311679E-446.018
CGAACTA405.6115823E-946.044
TACCCGA253.4173387E-546.020
GGTAATC206.311679E-446.023
CTATCGA253.4173387E-546.044
GCGCGTA253.4173387E-546.027
CGCATAC253.4173387E-546.024
CACGCAA301.861541E-646.034
GATTTCG301.861541E-646.09
GTCTAGC301.861541E-646.010
TACGGTC206.311679E-446.020
TAACGTC206.311679E-446.023
CGATTCA206.311679E-446.010
CGTTATG650.046.019
TACGCTT206.311679E-446.021
Produced by FastQC (version 0.11.5)