##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527749_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1255636
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.286630838873684	33.0	31.0	34.0	31.0	34.0
2	32.61593726207277	34.0	31.0	34.0	31.0	34.0
3	32.79754482987108	34.0	31.0	34.0	31.0	34.0
4	36.437361623910114	37.0	37.0	37.0	35.0	37.0
5	36.31689518299889	37.0	37.0	37.0	35.0	37.0
6	36.15882787686878	37.0	37.0	37.0	35.0	37.0
7	36.55266972275405	37.0	37.0	37.0	35.0	37.0
8	36.60248750434043	37.0	37.0	37.0	35.0	37.0
9	38.640776467065294	39.0	39.0	39.0	38.0	39.0
10	37.97001360266829	39.0	38.0	39.0	35.0	39.0
11	36.67347384114505	39.0	35.0	39.0	33.0	39.0
12	36.264653131958624	39.0	35.0	39.0	32.0	39.0
13	36.24270091013638	39.0	35.0	39.0	32.0	39.0
14	36.913542619039276	40.0	35.0	40.0	33.0	40.0
15	37.078392941903545	40.0	35.0	40.0	33.0	40.0
16	37.194225077968454	40.0	35.0	40.0	33.0	40.0
17	37.1988346941311	40.0	35.0	40.0	33.0	40.0
18	37.185106989605266	40.0	35.0	40.0	33.0	40.0
19	37.16135727233052	40.0	35.0	40.0	33.0	40.0
20	37.10889143031898	40.0	35.0	40.0	33.0	40.0
21	37.02503751087099	39.0	35.0	40.0	33.0	40.0
22	37.07511651465871	39.0	35.0	40.0	33.0	40.0
23	37.072607029425726	39.0	35.0	40.0	33.0	40.0
24	36.95144054487128	39.0	35.0	40.0	33.0	40.0
25	36.89387131302384	39.0	35.0	40.0	33.0	40.0
26	36.730329490393714	39.0	35.0	40.0	32.0	40.0
27	36.6411619290941	39.0	35.0	40.0	32.0	40.0
28	36.51739596507268	38.0	35.0	40.0	32.0	40.0
29	36.425282486325656	38.0	35.0	40.0	31.0	40.0
30	36.243678900573094	38.0	35.0	40.0	31.0	40.0
31	36.10727790538022	38.0	35.0	40.0	31.0	40.0
32	35.9937688948071	38.0	35.0	40.0	31.0	40.0
33	35.88178580416618	38.0	35.0	40.0	30.0	40.0
34	35.62999627280518	38.0	34.0	40.0	29.0	40.0
35	35.53797438111045	38.0	34.0	40.0	29.0	40.0
36	35.434460305375126	37.0	34.0	40.0	29.0	40.0
37	35.33965655651797	37.0	34.0	40.0	29.0	40.0
38	35.179843521530124	37.0	34.0	40.0	29.0	40.0
39	35.035643291527165	36.0	34.0	40.0	28.0	40.0
40	34.722260272881634	36.0	34.0	40.0	26.0	40.0
41	34.54283406974633	35.0	33.0	40.0	26.0	40.0
42	34.47363248584781	35.0	33.0	40.0	26.0	40.0
43	34.352381581923424	35.0	33.0	40.0	26.0	40.0
44	34.193446986228494	35.0	33.0	40.0	25.0	40.0
45	34.05734703369448	35.0	33.0	39.0	24.0	40.0
46	33.9135967748615	35.0	33.0	39.0	24.0	40.0
47	33.71319634034067	35.0	33.0	39.0	23.0	40.0
48	33.51805539184923	35.0	33.0	39.0	23.0	40.0
49	33.81026348400333	35.0	33.0	39.0	25.0	40.0
50	33.87455440908034	35.0	33.0	39.0	26.0	40.0
51	33.86765511661023	35.0	33.0	39.0	26.0	40.0
52	33.42723767078994	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	8.0
15	31.0
16	71.0
17	202.0
18	452.0
19	808.0
20	1454.0
21	2346.0
22	3609.0
23	5225.0
24	7129.0
25	9440.0
26	12235.0
27	15915.0
28	19506.0
29	23076.0
30	28445.0
31	36518.0
32	47889.0
33	69897.0
34	143472.0
35	119099.0
36	116997.0
37	174028.0
38	285774.0
39	132007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.9775699326875	0.8105852333000966	0.17513037217792418	0.03671446183448069
8	99.11726009767162	0.7109544485822324	0.10910805360789272	0.06267740013825662
9	97.07494847232797	1.3808938259177024	0.7645527844056716	0.7796049173486583
10	59.19621610084451	28.285267386408165	5.375921047182464	7.1425954655648605
11	38.29167051597756	26.102309905099887	18.7866547311482	16.819364847774352
12	32.93080160173808	16.4856694137473	25.827548748204098	24.755980236310524
13	26.04616305999509	17.4983036485096	28.227607363917567	28.227925927577736
14	24.11447266564514	18.69634193349028	31.260413049641777	25.928772351222808
15	25.455784956786843	20.341245392773065	28.77848357326486	25.424486077175235
16	29.327607682481226	21.384143175251427	24.499695771704538	24.78855337056281
17	31.61091271674275	20.984743986314506	22.434208640083593	24.970134656859152
18	30.084514939042844	20.638704210455895	23.63129123408376	25.6454896164175
19	30.340082635413445	22.69168771841521	21.631268934627553	25.336960711543792
20	29.94992179262143	22.947016492040685	22.944547623674378	24.15851409166351
21	28.73316789260582	21.483694319054248	25.101940371254088	24.681197417085844
22	29.355243080000893	21.100143672210734	24.469272942158398	25.07534030562997
23	28.12566699266348	22.44169488609756	23.687199156443427	25.745438964795532
24	28.301036287586527	23.287799967506505	22.26130821352685	26.149855531380112
25	30.161686985718788	23.312170087509436	19.747920575708246	26.778222351063523
26	27.332523119757635	22.571270654871316	22.749825586396057	27.34638063897499
27	27.519838551937028	21.1920492881695	23.466753103606457	27.821359056287015
28	28.717876836917704	21.866368915832297	21.39234618950078	28.023408057749222
29	31.191842221790388	21.98790095218678	21.158440822021667	25.66181600400116
30	28.633935312463166	22.01434173598081	22.240362652870736	27.111360298685288
31	30.174827736700767	21.602598205212338	22.43627930387469	25.786294754212207
32	31.67860749452867	21.352047886489398	22.62678037265577	24.342564246326166
33	33.13770869901787	21.788161537260798	21.35252573197965	23.721604031741684
34	28.832559754578558	23.085193479639006	25.536301921894562	22.545944843887877
35	28.823321408433657	25.883536311478807	24.088191163681195	21.20495111640635
36	29.41194741151098	25.693274165442855	22.59086231997171	22.30391610307446
37	30.04453519969163	25.336801429713706	20.286532084139033	24.33213128645563
38	30.694962552841748	24.346148087503067	21.026714748541774	23.932174611113414
39	30.723872205002085	22.052489734286052	21.56070708390011	25.662930976811747
40	29.753447655212177	23.12525285990526	22.06085203036549	25.06044745451707
41	27.841826771452872	23.552367087276885	22.929654772561474	25.676151368708766
42	27.5993201851492	22.09621259664425	22.477772220611705	27.826694997594842
43	26.817007476689103	22.19074636279941	24.854814611877966	26.137431548633522
44	27.913901799566116	20.794481840278554	24.34877623769946	26.94284012245587
45	28.7889165331354	21.7768525273248	23.316629978751806	26.117600960788
46	26.71817310112166	24.975709520912112	22.216390737443017	26.08972664052321
47	26.47996712423027	23.23396270893794	24.957551392282475	25.328518774549313
48	28.297054241834417	23.26852686606628	24.27184311376864	24.162575778330663
49	28.748140384633764	23.45257702072894	22.748630972670423	25.050651621966875
50	25.611164382034286	23.68934946114957	23.559534769630687	27.139951387185455
51	27.252324718310085	23.700180625595316	22.39733489641903	26.650159759675574
52	26.747082753282005	25.006849118693637	22.63554087331042	25.610527254713944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	8.0
4	16.0
5	45.0
6	74.0
7	203.5
8	333.0
9	1036.0
10	1739.0
11	2386.5
12	3034.0
13	3398.0
14	3159.0
15	2556.0
16	2753.0
17	2950.0
18	3119.5
19	3289.0
20	3361.5
21	3434.0
22	3867.5
23	4301.0
24	4585.5
25	4870.0
26	5592.0
27	6314.0
28	8926.0
29	11538.0
30	11582.0
31	11626.0
32	12209.0
33	12792.0
34	16703.5
35	20615.0
36	20701.0
37	20787.0
38	21443.0
39	23599.0
40	25099.0
41	27213.0
42	29327.0
43	34980.5
44	40634.0
45	39484.0
46	38334.0
47	43601.0
48	48868.0
49	49397.0
50	49926.0
51	57347.5
52	64769.0
53	69044.5
54	73320.0
55	90099.0
56	106878.0
57	109048.0
58	111218.0
59	114993.5
60	118769.0
61	115855.0
62	112941.0
63	101632.0
64	80087.5
65	69852.0
66	55799.5
67	41747.0
68	38503.5
69	35260.0
70	29906.5
71	24553.0
72	23089.0
73	21625.0
74	15473.0
75	9321.0
76	6621.0
77	3921.0
78	2661.0
79	1401.0
80	1254.0
81	1107.0
82	669.0
83	231.0
84	137.0
85	43.0
86	31.5
87	20.0
88	18.0
89	10.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1255636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.237131411134436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.94655331912324	7.365263209060551
2	9.279407524349798	1.8998902848847712
3	3.8645057887232106	1.186843607947477
4	2.0150640254586105	0.8251390092187739
5	1.1420020468581848	0.5845412512735872
6	0.727688647328174	0.44696665854535067
7	0.4620775615220776	0.3311244103606646
8	0.37878832113962246	0.3102164656407446
9	0.2823294923424904	0.2601219702914053
>10	4.353866495716674	12.273293108823202
>50	3.3381186609790494	24.563701119010712
>100	2.0991570983183725	36.992569386412505
>500	0.07699761123879914	5.277430128621442
>1k	0.03188789960394712	6.1885975530280595
>5k	7.77753648876759E-4	0.5968289945256903
>10k+	7.77753648876759E-4	0.897472842355067
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	11272	0.8977123943563262	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7496	0.596988299156762	No Hit
TCAAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	4647	0.37009133220137047	No Hit
TCAAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4511	0.35926016775562347	No Hit
TCAAAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	3854	0.30693608657286026	No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3838	0.30566183193218416	No Hit
TCAAAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	3761	0.2995294814739303	No Hit
TCAAAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	3190	0.2540545189848013	No Hit
TCAAAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2487	0.1980669557100943	No Hit
TCAAAGGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2370	0.1887489686501502	No Hit
TCAAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2291	0.18245733636181186	No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2183	0.17385611753724806	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC	2162	0.17218365832136065	No Hit
TCAAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2081	0.1657327442029378	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2020	0.1608746483853601	No Hit
TCAAAGGGGATTGACAGTCTTGCATTTTTGTTTTGTCTCGTGATGGTGGTGG	1821	0.14502610629195087	No Hit
TCAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1768	0.1408051377947112	No Hit
TCAAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	1736	0.13825662851335896	No Hit
TCAAAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	1700	0.1353895555718377	No Hit
TCAAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1683	0.1340356600161193	No Hit
TCAAAGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1661	0.13228355988518964	No Hit
TCAAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1644	0.13092966432947128	No Hit
TCAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	1573	0.125275159361471	No Hit
TCAAAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1547	0.1232044955703723	No Hit
TCAAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	1531	0.12193024092969618	No Hit
TCAAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1515	0.12065598628902008	No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1482	0.11802783609262557	No Hit
TCAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	1470	0.11707214511211848	No Hit
TCAAAGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	1409	0.11221404929454078	No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1336	0.10640026249645598	No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1261	0.10042719386828666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.04053722575650905	0.0	0.0	0.0	0.0
6	0.04053722575650905	0.0	0.0	0.0	0.0
7	0.07462353739459525	0.0	0.0	0.0	0.0
8	0.1470171291680073	0.0	0.0	0.0	0.0
9	0.21694185257510934	0.0	0.0	0.0	0.0
10	0.29180431271483137	0.0	0.0	0.0	0.0
11	0.3430930620020452	0.0	0.0	0.0	0.0
12	0.3728787642278495	0.0	0.0	0.0	0.0
13	0.3907979701123574	0.0	0.0	0.0	0.0
14	0.41564593560554175	0.0	0.0	0.0	0.0
15	0.43531724162097934	0.0	0.0	0.0	0.0
16	0.4582538251531495	0.0	0.0	0.0	0.0
17	0.47999579495968575	0.0	0.0	0.0	0.0
18	0.5016581238511798	0.0	0.0	0.0	0.0
19	0.5320013124822799	0.0	0.0	0.0	0.0
20	0.5590792235966474	0.0	0.0	0.0	0.0
21	0.5823343707889866	0.0	0.0	0.0	0.0
22	0.6032002905300581	0.0	0.0	0.0	0.0
23	0.628526101513496	0.0	0.0	0.0	0.0
24	0.6541704761571029	0.0	0.0	0.0	0.0
25	0.6786202370750759	0.0	0.0	0.0	0.0
26	0.7041849708036405	0.0	0.0	0.0	0.0
27	0.7251305314597543	0.0	0.0	0.0	0.0
28	0.7500581378679808	0.0	0.0	0.0	0.0
29	0.7678180619224043	0.0	0.0	0.0	0.0
30	0.7861354723821234	0.0	0.0	0.0	0.0
31	0.802063655390575	0.0	0.0	0.0	0.0
32	0.820619988595421	0.0	0.0	0.0	0.0
33	0.837026017094126	0.0	0.0	0.0	0.0
34	0.8535913274229155	0.0	0.0	0.0	0.0
35	0.8758907836347477	0.0	0.0	0.0	0.0
36	0.8950842441599317	0.0	0.0	0.0	0.0
37	0.9138795001099045	0.0	0.0	0.0	0.0
38	0.9308430150139053	0.0	0.0	0.0	0.0
39	0.9497179118789203	0.0	0.0	0.0	0.0
40	0.9670796313581325	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	70	0.0	46.000004	38
ATAGCCG	70	0.0	46.000004	15
ACGTGCG	55	1.8189894E-12	46.000004	31
CGATAAT	35	1.0196709E-7	46.000004	14
ATTTACG	20	6.311679E-4	46.0	44
GTCGCAA	20	6.311679E-4	46.0	18
CGAACTA	40	5.6115823E-9	46.0	44
TACCCGA	25	3.4173387E-5	46.0	20
GGTAATC	20	6.311679E-4	46.0	23
CTATCGA	25	3.4173387E-5	46.0	44
GCGCGTA	25	3.4173387E-5	46.0	27
CGCATAC	25	3.4173387E-5	46.0	24
CACGCAA	30	1.861541E-6	46.0	34
GATTTCG	30	1.861541E-6	46.0	9
GTCTAGC	30	1.861541E-6	46.0	10
TACGGTC	20	6.311679E-4	46.0	20
TAACGTC	20	6.311679E-4	46.0	23
CGATTCA	20	6.311679E-4	46.0	10
CGTTATG	65	0.0	46.0	19
TACGCTT	20	6.311679E-4	46.0	21
>>END_MODULE