FastQCFastQC Report
Fri 17 Jun 2016
SRR1527748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265318
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA106380.8407372692082148No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC73470.5806445494334231No Hit
TCAAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT44790.35398216100616603No Hit
TCAAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC42500.3358839437991082No Hit
TCAAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT37370.29534077599465114No Hit
TCAAAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG36260.2865682776977803No Hit
TCAAAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC35960.28419733221213955No Hit
TCAAAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA30220.23883324192021296No Hit
TCAAAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG23370.1846966533314155No Hit
TCAAAGGGGGTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC22730.17963863629538188No Hit
TCAAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG22410.17710962777736505No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC21400.1691274446423745No Hit
TCAAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT21060.16644037309198162No Hit
TCAAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG19820.15664046508466647No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT18840.14889537649823995No Hit
TCAAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT17740.14020190971755717No Hit
TCAAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC17220.13609227087577985No Hit
TCAAAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT16490.13032297019405398No Hit
TCAAAGGGGATTGACAGTCTTGCATTTTTGTTTTGTCTCGTGATGGTGGTGG16080.12708267803034493No Hit
TCAAAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA15760.12455366951232812No Hit
TCAAAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA15470.12226175554287538No Hit
TCAAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14930.11799405366872201No Hit
TCAAAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT14870.11751986457159386No Hit
TCAAAGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC14780.11680858092590163No Hit
TCAAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT14700.11617632879639742No Hit
TCAAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT14430.11404247785932074No Hit
TCAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13620.10764092504809068No Hit
TCAAAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG13540.10700867291858647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT351.0196709E-746.00000433
CATACTC351.0196709E-746.00000442
TCGTTAC206.3116965E-446.043
ACACGTA206.3116965E-446.028
CTCGTTA206.3116965E-446.042
GATAACG206.3116965E-446.024
CGAAACG253.4173518E-546.038
GTAGGGT206.3116965E-446.035
TTATCGA253.4173518E-546.020
ACTAGGC206.3116965E-446.041
CCCGTAA253.4173518E-546.033
TCTACGG600.046.011
ACGTGCG501.6370905E-1146.031
TATAGCG206.3116965E-446.012
CACGTAT206.3116965E-446.029
TATACGG253.4173518E-546.044
TAAGCGC405.6115823E-946.034
TTCGAAT206.3116965E-446.026
TATCGAA253.4173518E-546.021
ATACCCG253.4173518E-546.034
Produced by FastQC (version 0.11.5)