FastQCFastQC Report
Fri 17 Jun 2016
SRR1527747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696785
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT51160.7342293533873433No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC34460.4945571445998407No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG31510.45221983825713813No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC29080.4173453791341662No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT28800.41332692293892664No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC28630.41088714596324544No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22930.3290828591315829No Hit
AACGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC22690.32563846810709185No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT18460.264931076300437No Hit
AACGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT17610.2527321914220312No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG16870.24211198576318377No Hit
AACGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG15950.22890848683596804No Hit
AACGCCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC15380.22072805815280178No Hit
AACGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA14050.20164039122541386No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT14010.20106632605466535No Hit
AACGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA13980.20063577717660397No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGC12770.18327030576146158No Hit
AACGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA11840.16992329054155872No Hit
AACGCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC11700.16791406244393894No Hit
AACGCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC11450.16432615512676077No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC11400.16360857366332512No Hit
AACGCCGGGCCTGTGTTACAATTTTTCGTTTGGTCTTAGGTCTTCCCATG11330.16260395961451524No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG10880.1561457264435945No Hit
AACGCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG10680.15327540058985195No Hit
AACGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA9970.1430857438090659No Hit
AACGCCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC9670.13878025502845212No Hit
AACGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC9490.1361969617600838No Hit
AACGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA8940.1283035656622918No Hit
AACGCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG8690.12471565834511363No Hit
AACGCCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC7950.1140954526862662No Hit
AACGCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG7800.1119427082959593No Hit
AACGCCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG7710.11065106166177514No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC7510.10778073580803262No Hit
AACGCCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT7430.10663260546653558No Hit
AACGCCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT7260.10419282849085441No Hit
AACGCCGGGATCTTACTACTGCATGCGCTCCAGGATGGTCCTGCATTCCT7130.10232711668592177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA650.044.00000434
TACTATC650.044.00000413
AACGTTA207.8543444E-444.020
AACCGTC207.8543444E-444.012
CCTTATC207.8543444E-444.012
TGATACC454.783942E-1044.011
AATCCCC254.4410284E-544.015
CTACTTA207.8543444E-444.012
AATTTAC207.8543444E-444.041
TACCATT302.5262761E-644.021
GATCGAC254.4410284E-544.09
CGAGTCA207.8543444E-444.012
CTATAGC207.8543444E-444.035
CTATACG207.8543444E-444.017
ATTCGGC207.8543444E-444.010
ATTCGCG254.4410284E-544.018
TTCCGTA254.4410284E-544.036
TGACGTA207.8543444E-444.019
TCGATTG254.4410284E-544.021
GGATACG254.4410284E-544.09
Produced by FastQC (version 0.11.5)