##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527747_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	696785
Sequences flagged as poor quality	0
Sequence length	50
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.674162044246074	31.0	31.0	34.0	3.0	34.0
2	30.368925852307385	34.0	31.0	34.0	16.0	34.0
3	31.929048415221338	34.0	31.0	34.0	28.0	34.0
4	35.957491909269	37.0	35.0	37.0	35.0	37.0
5	36.11373092130284	37.0	35.0	37.0	35.0	37.0
6	36.46210954598621	37.0	37.0	37.0	35.0	37.0
7	36.46151682369741	37.0	37.0	37.0	35.0	37.0
8	36.62387680561436	37.0	37.0	37.0	35.0	37.0
9	38.71248376471939	39.0	39.0	39.0	39.0	39.0
10	38.23880250005382	39.0	39.0	39.0	37.0	39.0
11	37.95407478634012	39.0	38.0	39.0	35.0	39.0
12	37.39222141693636	39.0	37.0	39.0	35.0	39.0
13	37.253392366368395	39.0	37.0	39.0	35.0	39.0
14	38.01679284140732	40.0	38.0	40.0	34.0	40.0
15	38.031926634471176	40.0	38.0	40.0	34.0	40.0
16	38.036628228219605	40.0	38.0	40.0	34.0	40.0
17	37.97840223311351	40.0	38.0	40.0	34.0	40.0
18	37.87417783103827	40.0	37.0	40.0	33.0	40.0
19	37.78518050761713	40.0	37.0	40.0	33.0	40.0
20	37.63578435241861	40.0	37.0	40.0	33.0	40.0
21	37.62117439382306	40.0	37.0	40.0	33.0	40.0
22	37.649577703308765	40.0	37.0	40.0	33.0	40.0
23	37.52424923039388	40.0	36.0	40.0	33.0	40.0
24	37.40067022108685	39.0	35.0	40.0	33.0	40.0
25	37.33618261013081	39.0	35.0	40.0	33.0	40.0
26	37.17175886392503	39.0	35.0	40.0	33.0	40.0
27	36.97092359910159	39.0	35.0	40.0	33.0	40.0
28	36.68169808477507	38.0	35.0	40.0	32.0	40.0
29	36.67923821551842	39.0	35.0	40.0	32.0	40.0
30	36.822727240109934	39.0	35.0	40.0	32.0	40.0
31	36.80871143896611	39.0	35.0	40.0	33.0	40.0
32	36.717912986071745	39.0	35.0	40.0	33.0	40.0
33	36.45927222887978	38.0	35.0	40.0	32.0	40.0
34	36.05958939988662	38.0	35.0	40.0	31.0	40.0
35	35.98576605409129	37.0	35.0	40.0	31.0	40.0
36	35.86169047841156	37.0	35.0	40.0	31.0	40.0
37	35.69926160867413	37.0	35.0	40.0	31.0	40.0
38	35.604869507810875	36.0	35.0	40.0	31.0	40.0
39	35.42799572321448	36.0	35.0	40.0	31.0	40.0
40	35.13473596590053	35.0	34.0	40.0	30.0	40.0
41	34.97039402398157	35.0	34.0	39.0	30.0	40.0
42	34.85290728129911	35.0	34.0	39.0	30.0	40.0
43	34.676508535631505	35.0	34.0	39.0	29.0	40.0
44	34.266671928930734	35.0	34.0	39.0	28.0	40.0
45	34.23733863386841	35.0	34.0	39.0	28.0	40.0
46	34.06070595664373	35.0	34.0	38.0	28.0	40.0
47	33.93348306866537	35.0	34.0	37.0	27.0	40.0
48	33.835798704047875	35.0	33.0	37.0	27.0	40.0
49	33.72417316675875	35.0	33.0	37.0	27.0	40.0
50	33.271565834511364	35.0	33.0	36.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	5.0
15	14.0
16	31.0
17	52.0
18	114.0
19	210.0
20	406.0
21	650.0
22	918.0
23	1483.0
24	2409.0
25	3310.0
26	4515.0
27	5692.0
28	7782.0
29	9957.0
30	12934.0
31	16247.0
32	21223.0
33	32558.0
34	58743.0
35	80599.0
36	113832.0
37	143293.0
38	133892.0
39	45910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.12455061460852	0.4055770431338218	0.19489512546911889	0.27497721678853587
8	98.93611372231032	0.6717997660684429	0.17336768156604979	0.218718830055182
9	96.78336933200342	1.4041634076508536	0.8013949783649188	1.0110722819808118
10	58.82374046513631	27.122426573476755	5.710513286020795	8.343319675366146
11	38.13916774901871	23.201848489849812	20.595736130944264	18.063247630187217
12	31.439540173798232	16.838910137273334	25.62985712953063	26.091692559397806
13	25.491220390794865	15.994173238516904	29.642285640477333	28.872320730210898
14	26.18454760076638	17.685799780420073	29.78795467755477	26.34169794125878
15	26.700775705561973	18.102571094383492	27.196050431625252	28.00060276842928
16	31.358453468430003	18.218245226289316	24.813392940433562	25.60990836484712
17	31.83320536463902	19.04733884914285	22.73427240827515	26.385183377942983
18	32.10976126064711	19.766929540676106	22.268418522212734	25.85489067646404
19	31.52852027526425	19.899969143997073	22.022718629132374	26.548791951606304
20	30.20257324712788	21.695214449220348	21.885086504445418	26.217125799206354
21	30.30748365708217	20.749729112997553	24.315534921101918	24.62725230881836
22	30.78596697690105	19.028538214800836	24.10413542197378	26.081359386324333
23	30.57987758060234	20.686294911629844	22.915389969646306	25.818437538121515
24	30.153347158736192	20.47819628723351	22.744462065055934	26.62399448897436
25	30.047001585855032	19.791901375603665	22.111985763183764	28.049111275357536
26	27.967306988525877	19.81515101501898	22.257511283968512	29.960030712486635
27	26.882323815811187	18.55105950903076	23.59967565317853	30.966941021979522
28	29.147872012170183	21.045946741103783	22.06132451186521	27.744856734860825
29	30.197693693176518	21.316044403940957	22.09921281313461	26.387049089747915
30	30.18147635210287	19.458513027691467	23.544708913079358	26.8153017071263
31	32.63546144076006	20.606356336603113	21.03102104666432	25.7271611759725
32	34.28561177407665	20.027411611903243	21.574086698192414	24.112889915827694
33	34.36956880529862	19.80166048350639	22.137388146989387	23.6913825642056
34	30.345084925766198	23.005948750331882	24.76560201496875	21.88336430893317
35	29.032771945435105	25.17634564463931	24.748810608724355	21.04207180120123
36	30.35039502859562	24.452592980618125	21.198361043937513	23.99865094684874
37	30.79873992695021	25.56240447196768	20.46815014674541	23.170705454336705
38	32.54461562748911	24.24951742646584	20.092568008783196	23.113298937261852
39	32.0519241946942	22.017695558888324	19.84973844155658	26.0806418048609
40	28.6738376974246	23.515431589371182	21.68114985253701	26.129580860667208
41	27.64712213954089	22.608121587003165	22.155471199867964	27.589285073587977
42	26.86926383317666	20.47661760801395	22.65619954505335	29.997919013756036
43	26.13044195842333	19.17104989343915	25.105735628637245	29.592772519500276
44	27.87789633818179	19.89308036194809	23.92273082801725	28.306292471852867
45	28.183586041605373	20.58899086518797	23.26399104458334	27.96343204862332
46	27.73710685505572	21.339724592234333	23.389424284391886	27.53374426831806
47	27.144958631428633	21.477069684335916	23.82054722762402	27.557424456611436
48	28.929727247285747	21.998607891960937	24.95088154882783	24.120783311925486
49	27.87760930559642	21.11009852393493	24.741491277797312	26.270800892671343
50	26.255157616768443	21.98640900708253	24.663848963453578	27.09458441269545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.5
8	9.0
9	9.0
10	9.0
11	43.5
12	78.0
13	179.0
14	280.0
15	735.0
16	1190.0
17	1467.0
18	1744.0
19	1481.0
20	1218.0
21	1079.5
22	941.0
23	1189.5
24	1438.0
25	1551.5
26	1665.0
27	1966.0
28	2267.0
29	2197.0
30	2127.0
31	3119.0
32	4111.0
33	3795.0
34	3479.0
35	4420.5
36	5362.0
37	6629.5
38	7897.0
39	8139.0
40	8381.0
41	9184.0
42	9987.0
43	11255.5
44	12524.0
45	13849.5
46	15175.0
47	16306.0
48	17437.0
49	18692.0
50	19947.0
51	23374.0
52	26801.0
53	27066.0
54	27331.0
55	31135.5
56	34940.0
57	39299.0
58	43658.0
59	56148.0
60	68638.0
61	71233.0
62	73828.0
63	73329.5
64	72831.0
65	69787.5
66	66744.0
67	61464.5
68	56185.0
69	45036.5
70	33888.0
71	28347.0
72	22806.0
73	20520.5
74	18235.0
75	15519.0
76	12803.0
77	12559.0
78	12315.0
79	9186.0
80	6057.0
81	3688.0
82	1319.0
83	975.0
84	631.0
85	509.0
86	387.0
87	237.0
88	87.0
89	57.5
90	28.0
91	16.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	696785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.052311688684458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.34678697371615	7.071478289572823
2	9.411361592164813	1.8921188027870863
3	3.600645317876162	1.0858442704708051
4	1.7789072426937738	0.7152852027526425
5	0.9465613979983724	0.47575651025782706
6	0.6638779035735191	0.40041045659708513
7	0.4682837685421813	0.3295134080096443
8	0.3455020487414874	0.27784754264227846
9	0.25127421726653626	0.22732980761640967
>10	6.219036877346773	17.92274517964652
>50	4.107476835658096	28.921690334895267
>100	1.7674856873634768	30.094936027612533
>500	0.05853547106777266	3.873935288503627
>1k	0.03283697157460417	5.976879525248104
>5k	0.001427694416287138	0.7342293533873433
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5116	0.7342293533873433	No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3446	0.4945571445998407	No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3151	0.45221983825713813	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2908	0.4173453791341662	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	2880	0.41332692293892664	No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2863	0.41088714596324544	No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	2293	0.3290828591315829	No Hit
AACGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2269	0.32563846810709185	No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	1846	0.264931076300437	No Hit
AACGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1761	0.2527321914220312	No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1687	0.24211198576318377	No Hit
AACGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1595	0.22890848683596804	No Hit
AACGCCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	1538	0.22072805815280178	No Hit
AACGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1405	0.20164039122541386	No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1401	0.20106632605466535	No Hit
AACGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1398	0.20063577717660397	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGC	1277	0.18327030576146158	No Hit
AACGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	1184	0.16992329054155872	No Hit
AACGCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	1170	0.16791406244393894	No Hit
AACGCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1145	0.16432615512676077	No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1140	0.16360857366332512	No Hit
AACGCCGGGCCTGTGTTACAATTTTTCGTTTGGTCTTAGGTCTTCCCATG	1133	0.16260395961451524	No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1088	0.1561457264435945	No Hit
AACGCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1068	0.15327540058985195	No Hit
AACGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	997	0.1430857438090659	No Hit
AACGCCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	967	0.13878025502845212	No Hit
AACGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	949	0.1361969617600838	No Hit
AACGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	894	0.1283035656622918	No Hit
AACGCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	869	0.12471565834511363	No Hit
AACGCCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	795	0.1140954526862662	No Hit
AACGCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	780	0.1119427082959593	No Hit
AACGCCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	771	0.11065106166177514	No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	751	0.10778073580803262	No Hit
AACGCCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	743	0.10663260546653558	No Hit
AACGCCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	726	0.10419282849085441	No Hit
AACGCCGGGATCTTACTACTGCATGCGCTCCAGGATGGTCCTGCATTCCT	713	0.10232711668592177	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.006027684292859347	0.0	0.0	0.0	0.0
8	0.009328559024663275	0.0	0.0	0.0	0.0
9	0.011624819707657311	0.0	0.0	0.0	0.0
10	0.02396722087875026	0.0	0.0	0.0	0.0
11	0.03300874731803928	0.0	0.0	0.0	0.0
12	0.04291137151345106	0.0	0.0	0.0	0.0
13	0.05324454458692423	0.0	0.0	0.0	0.0
14	0.06860078790444685	0.0	0.0	0.0	0.0
15	0.0803691239047913	0.0	0.0	0.0	0.0
16	0.09859569307605646	0.0	0.0	0.0	0.0
17	0.1135213875155177	0.0	0.0	0.0	0.0
18	0.12701191902810766	0.0	0.0	0.0	0.0
19	0.15585869385822027	0.0	0.0	0.0	0.0
20	0.17064087200499437	0.0	0.0	0.0	0.0
21	0.1854230501517685	0.0	0.0	0.0	0.0
22	0.2039366519084079	0.0	0.0	0.0	0.0
23	0.2260381609822255	0.0	0.0	0.0	0.0
24	0.2448387953242392	0.0	0.0	0.0	0.0
25	0.26306536449550433	0.0	0.0	0.0	0.0
26	0.2864585202035061	0.0	0.0	0.0	0.0
27	0.3028193775698386	0.0	0.0	0.0	0.0
28	0.3309485709365156	0.0	0.0	0.0	0.0
29	0.3464483305467253	0.0	0.0	0.0	0.0
30	0.3662535789375489	0.0	0.0	0.0	0.0
31	0.37802191493789333	0.0	0.0	0.0	0.0
32	0.39768364703602976	0.0	0.0	0.0	0.0
33	0.4120352763047425	0.0	0.0	0.0	0.0
34	0.4309794269394433	0.0	0.0	0.0	0.0
35	0.4657103697697281	0.0	0.0	0.0	0.0
36	0.48752484625817144	0.0	0.0	0.0	0.0
37	0.5077606435270564	0.0	0.0	0.0	0.0
38	0.5245520497714503	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACTA	65	0.0	44.000004	34
TACTATC	65	0.0	44.000004	13
AACGTTA	20	7.8543444E-4	44.0	20
AACCGTC	20	7.8543444E-4	44.0	12
CCTTATC	20	7.8543444E-4	44.0	12
TGATACC	45	4.783942E-10	44.0	11
AATCCCC	25	4.4410284E-5	44.0	15
CTACTTA	20	7.8543444E-4	44.0	12
AATTTAC	20	7.8543444E-4	44.0	41
TACCATT	30	2.5262761E-6	44.0	21
GATCGAC	25	4.4410284E-5	44.0	9
CGAGTCA	20	7.8543444E-4	44.0	12
CTATAGC	20	7.8543444E-4	44.0	35
CTATACG	20	7.8543444E-4	44.0	17
ATTCGGC	20	7.8543444E-4	44.0	10
ATTCGCG	25	4.4410284E-5	44.0	18
TTCCGTA	25	4.4410284E-5	44.0	36
TGACGTA	20	7.8543444E-4	44.0	19
TCGATTG	25	4.4410284E-5	44.0	21
GGATACG	25	4.4410284E-5	44.0	9
>>END_MODULE