FastQCFastQC Report
Fri 17 Jun 2016
SRR1527746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884929
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG63730.7201707707624002No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT42410.4792474876515517No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT41800.47235427926986234No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC38180.4314470426440991No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA35120.3968679973195589No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC34150.3859066659585119No Hit
AACGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC29010.32782291008657194No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT26310.29731198774138945No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT23730.26815710638932616No Hit
AACGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23080.2608118843432637No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT21900.247477481244258No Hit
AACGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC19330.21843560330828798No Hit
AACGCCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT19020.21493249740939668No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT18910.21368945983237073No Hit
AACGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG17920.20250212163913714No Hit
AACGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA17800.2011460806460179No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC17130.19357485176776895No Hit
AACGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG15660.17696334960205848No Hit
AACGCCGGGCCTGTGTTACAATTTTTCGTTTGGTCTTAGGTCTTCCCATGGC14490.16374194991914606No Hit
AACGCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC14220.1606908576846278No Hit
AACGCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA13880.15684874153745668No Hit
AACGCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA13530.1528936219741923No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG13500.15255461172591248No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC13410.15153758098107306No Hit
AACGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG12750.14407935551891735No Hit
AACGCCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG12680.14328833160626447No Hit
AACGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT11800.13334403099005682No Hit
AACGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11060.12498177819915497No Hit
AACGCCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT11010.12441676111868863No Hit
AACGCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10570.11944461081058481No Hit
AACGCCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT10540.119105600562305No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT10140.11458546391857424No Hit
AACGCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG10140.11458546391857424No Hit
AACGCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC9450.10678822820813874No Hit
AACGCCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG9320.10531918379892624No Hit
AACGCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC9090.10272010522878107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCC700.046.00000414
GTCGATA351.01921614E-746.00000445
CATTCCG351.01921614E-746.00000434
TCGATAG351.01921614E-746.00000446
CACCTCG351.01921614E-746.00000426
GTTACGG351.01921614E-746.00000426
CGAGGTA351.01921614E-746.00000432
TGTTACG351.01921614E-746.00000425
TACTATC700.046.00000413
TGATACC453.092282E-1046.011
CGTATGT253.416514E-546.034
CGTATGA206.3106645E-446.013
ATAGGTT453.092282E-1046.016
TACCATT405.6097633E-946.021
TTTACGC301.8609135E-646.037
CGGTTTA206.3106645E-446.038
ATAATAC301.8609135E-646.013
TAGCGTC551.8189894E-1246.043
CTATACG301.8609135E-646.017
CGGTTAT206.3106645E-446.039
Produced by FastQC (version 0.11.5)