##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527746_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	884929
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.934531470886366	31.0	31.0	33.0	31.0	34.0
2	32.638797010833635	34.0	31.0	34.0	31.0	34.0
3	32.38137183887069	34.0	31.0	34.0	31.0	34.0
4	36.009287750768706	37.0	35.0	37.0	35.0	37.0
5	36.06087494024944	37.0	37.0	37.0	35.0	37.0
6	36.299289547523024	37.0	37.0	37.0	35.0	37.0
7	36.38112888152609	37.0	35.0	37.0	35.0	37.0
8	36.54628676424888	37.0	37.0	37.0	35.0	37.0
9	38.63543176910238	39.0	39.0	39.0	38.0	39.0
10	37.976465908564414	39.0	38.0	39.0	35.0	39.0
11	37.60358175627649	39.0	37.0	39.0	35.0	39.0
12	37.02929048545138	39.0	37.0	39.0	34.0	39.0
13	36.90300238776218	39.0	37.0	39.0	33.0	39.0
14	37.66290628965714	40.0	37.0	40.0	33.0	40.0
15	37.71139944560524	40.0	37.0	40.0	33.0	40.0
16	37.71109433638179	40.0	37.0	40.0	33.0	40.0
17	37.65092114734628	40.0	37.0	40.0	33.0	40.0
18	37.62754187059075	40.0	37.0	40.0	33.0	40.0
19	37.549596634306255	40.0	37.0	40.0	33.0	40.0
20	37.479920988011465	40.0	36.0	40.0	33.0	40.0
21	37.44390114913174	40.0	36.0	40.0	33.0	40.0
22	37.4422592094959	40.0	36.0	40.0	33.0	40.0
23	37.36098602260746	40.0	35.0	40.0	33.0	40.0
24	37.22644415540682	39.0	35.0	40.0	33.0	40.0
25	37.17444789355982	39.0	35.0	40.0	33.0	40.0
26	37.010659612240076	39.0	35.0	40.0	32.0	40.0
27	36.844389775902926	39.0	35.0	40.0	32.0	40.0
28	36.61801794268241	38.0	35.0	40.0	32.0	40.0
29	36.46641481972	38.0	35.0	40.0	31.0	40.0
30	36.26569702202097	38.0	35.0	40.0	31.0	40.0
31	36.079521633938995	38.0	35.0	40.0	31.0	40.0
32	35.87282934563112	37.0	35.0	40.0	30.0	40.0
33	35.57527553057929	37.0	34.0	40.0	30.0	40.0
34	35.29165729680008	37.0	34.0	40.0	29.0	40.0
35	35.13053137596349	36.0	34.0	40.0	29.0	40.0
36	35.0522392191916	36.0	34.0	40.0	29.0	40.0
37	34.898617855217765	36.0	34.0	39.0	29.0	40.0
38	34.78491381794472	35.0	34.0	39.0	29.0	40.0
39	34.63723756369155	35.0	34.0	39.0	28.0	40.0
40	34.28237519620218	35.0	33.0	39.0	27.0	40.0
41	34.14269054353513	35.0	33.0	39.0	27.0	40.0
42	34.03859292666417	35.0	33.0	38.0	27.0	40.0
43	33.83533820227385	35.0	33.0	38.0	26.0	40.0
44	33.62825831224878	35.0	33.0	38.0	26.0	40.0
45	33.5204993846964	35.0	33.0	37.0	26.0	40.0
46	33.37372828780614	35.0	33.0	37.0	25.0	40.0
47	33.12082325248692	35.0	32.0	37.0	24.0	40.0
48	32.93221151075397	35.0	32.0	36.0	24.0	39.0
49	33.233041294838344	35.0	33.0	36.0	26.0	39.0
50	33.319005253528815	35.0	33.0	36.0	26.0	39.0
51	33.339027198792216	35.0	33.0	36.0	27.0	39.0
52	32.92067612203917	35.0	33.0	35.0	25.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	5.0
15	12.0
16	37.0
17	80.0
18	176.0
19	414.0
20	659.0
21	1196.0
22	1983.0
23	2833.0
24	4220.0
25	5839.0
26	7653.0
27	9956.0
28	12513.0
29	15681.0
30	19440.0
31	24769.0
32	32173.0
33	46192.0
34	83637.0
35	102009.0
36	147195.0
37	163413.0
38	161725.0
39	41116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.23496687304856	0.37618837217449086	0.1474694580017154	0.24137529677522152
8	99.05325737997059	0.6349661950280757	0.1252077850313415	0.1865686399699863
9	96.8439275919311	1.3827097993172335	0.7337311806935923	1.03963142805807
10	59.07592586523891	26.99809815250715	5.663618211178524	8.26235777107542
11	38.162383648857706	23.223784054991985	20.445821077171164	18.16801121897915
12	31.544790599019805	16.773775071220403	25.540580091736174	26.140854238023614
13	25.575159137060716	16.019025255133464	29.535476857465405	28.870338750340423
14	26.34527741773634	17.781652539356266	29.554348427952977	26.31872161495442
15	26.896621084855393	17.850132609508783	27.23834341512144	28.01490289051438
16	31.26996629108098	18.049696642329497	25.062010624581184	25.618326442008343
17	31.987424979857142	18.832132295359287	22.762730117331447	26.41771260745212
18	32.17195955833745	19.6058666853499	22.38055256410401	25.841621192208642
19	31.628074116680548	19.825206315986932	22.004138185097336	26.542581382235188
20	30.32367568471595	21.563085852085308	21.88333753329363	26.22990092990511
21	30.344920326941484	20.61543920472716	24.307034801662052	24.7326056666693
22	30.73546013295982	18.949316837848006	24.125664318832357	26.18955871035981
23	30.768118120210776	20.741890027335526	22.89562213465713	25.594369717796567
24	30.31960756173659	20.29835161916945	22.595598064929504	26.786442754164458
25	29.995852774629377	19.623721225092634	22.067307094693472	28.313118905584517
26	28.268821566475953	19.681465970716296	22.200990135931807	29.848722326875944
27	26.805540331484224	18.462611124734302	23.538611572227826	31.193236971553652
28	29.103125787492555	20.950268326611514	22.03012897079879	27.916476915097142
29	30.161402779206014	21.13435089142745	22.14855655086453	26.555689778502007
30	30.241183190967863	19.311944800091307	23.53725553123471	26.909616477706123
31	32.84851101048785	20.43565076972277	20.976146108896874	25.739692110892516
32	34.410670234561195	19.860237374975846	21.572804145869327	24.156288244593636
33	34.43677402367874	19.63637760769508	22.261672970373894	23.665175398252288
34	30.19236571521557	22.869179335291307	24.83735983338776	22.10109511610536
35	29.10289978066037	25.035567825215356	24.734413721326796	21.12711867279748
36	30.35102251141052	24.29166633707337	21.16734788892668	24.18996326258943
37	30.862927986313025	25.403619951431132	20.488875378702698	23.244576683553145
38	32.52927636002436	24.17402978092028	20.120484242238643	23.176209616816717
39	32.18359891019505	21.860510843242793	19.771303686510443	26.18458656005171
40	28.695296458811946	23.37057549249714	21.76570097714054	26.168427071550372
41	27.824944148061597	22.583619702823615	22.006850267083575	27.584585882031217
42	26.767571183676882	20.509555003847765	22.528926049434475	30.193947763040878
43	26.081753451406836	19.089893087468035	24.88809836721364	29.94025509391149
44	27.722337046248906	19.731865494293892	23.94451984283485	28.60127761662235
45	28.214353919919	20.346717081257367	23.25553801491419	28.183390983909444
46	27.553283935773376	21.199666865929355	23.52414713496789	27.722902063329375
47	27.39270608150484	21.25854164571395	23.80010147706765	27.548650795713552
48	29.14855316076205	21.68512954146604	24.923242429618647	24.243074868153265
49	27.847657834696342	20.910378120730588	24.85137225698333	26.39059178758974
50	26.216566526806105	21.907181254089313	24.91849628614273	26.957755932961852
51	26.696718041786404	22.012161427639956	23.864174413992533	27.426946116581103
52	27.70775960557288	21.109151129638647	25.2295946906475	25.95349457414098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	7.0
8	14.0
9	13.0
10	12.0
11	57.5
12	103.0
13	244.0
14	852.0
15	1319.0
16	1583.0
17	1847.0
18	1795.5
19	1744.0
20	1371.0
21	998.0
22	1269.5
23	1541.0
24	1558.0
25	1575.0
26	1915.5
27	2256.0
28	2406.5
29	2557.0
30	2985.0
31	3413.0
32	4193.0
33	4973.0
34	4923.5
35	4874.0
36	5822.0
37	6770.0
38	8785.0
39	10577.0
40	10354.0
41	11077.5
42	11801.0
43	14599.5
44	17398.0
45	18251.0
46	19104.0
47	20012.5
48	20921.0
49	23577.5
50	26234.0
51	26537.0
52	26840.0
53	31896.0
54	36952.0
55	38607.5
56	40263.0
57	47612.0
58	54961.0
59	66039.0
60	77117.0
61	87265.5
62	97414.0
63	92281.0
64	86657.5
65	86167.0
66	77545.5
67	68924.0
68	60809.0
69	52694.0
70	41145.0
71	29596.0
72	27317.5
73	25039.0
74	23129.0
75	21219.0
76	18659.0
77	16099.0
78	12588.5
79	9078.0
80	6011.5
81	2945.0
82	1865.5
83	786.0
84	633.5
85	481.0
86	315.5
87	150.0
88	92.5
89	28.5
90	22.0
91	13.5
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	884929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.785563587587253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.69619015769301	6.123203104429846
2	9.348390914002007	1.6426176563317507
3	4.207290407223523	1.1089025221232438
4	2.1261544002263784	0.7471785872086913
5	1.1923443006714172	0.5237708335922995
6	0.7344429295397834	0.3871497035355379
7	0.47590872842332727	0.29267884768156543
8	0.37815450312556276	0.2657840346513675
9	0.26496540014920383	0.2095083334369198
>10	4.788670799783912	11.710657013161507
>50	4.023358114886939	25.600810912513882
>100	2.634219123813444	38.56930895020956
>500	0.08746430684536825	5.0335111630424585
>1k	0.04115967380958506	7.06474756731896
>5k	0.0012862398065495331	0.7201707707624002
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6373	0.7201707707624002	No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4241	0.4792474876515517	No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4180	0.47235427926986234	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3818	0.4314470426440991	No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3512	0.3968679973195589	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3415	0.3859066659585119	No Hit
AACGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2901	0.32782291008657194	No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2631	0.29731198774138945	No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	2373	0.26815710638932616	No Hit
AACGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2308	0.2608118843432637	No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2190	0.247477481244258	No Hit
AACGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1933	0.21843560330828798	No Hit
AACGCCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1902	0.21493249740939668	No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1891	0.21368945983237073	No Hit
AACGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	1792	0.20250212163913714	No Hit
AACGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1780	0.2011460806460179	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC	1713	0.19357485176776895	No Hit
AACGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1566	0.17696334960205848	No Hit
AACGCCGGGCCTGTGTTACAATTTTTCGTTTGGTCTTAGGTCTTCCCATGGC	1449	0.16374194991914606	No Hit
AACGCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1422	0.1606908576846278	No Hit
AACGCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1388	0.15684874153745668	No Hit
AACGCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1353	0.1528936219741923	No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1350	0.15255461172591248	No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1341	0.15153758098107306	No Hit
AACGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1275	0.14407935551891735	No Hit
AACGCCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1268	0.14328833160626447	No Hit
AACGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1180	0.13334403099005682	No Hit
AACGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1106	0.12498177819915497	No Hit
AACGCCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1101	0.12441676111868863	No Hit
AACGCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1057	0.11944461081058481	No Hit
AACGCCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1054	0.119105600562305	No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1014	0.11458546391857424	No Hit
AACGCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1014	0.11458546391857424	No Hit
AACGCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	945	0.10678822820813874	No Hit
AACGCCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG	932	0.10531918379892624	No Hit
AACGCCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	909	0.10272010522878107	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.005537167388570156	0.0	0.0	0.0	0.0
8	0.009492286951834555	0.0	0.0	0.0	0.0
9	0.011074334777140312	0.0	0.0	0.0	0.0
10	0.020227611480695063	0.0	0.0	0.0	0.0
11	0.028137850607223857	0.0	0.0	0.0	0.0
12	0.037404130726871876	0.0	0.0	0.0	0.0
13	0.04746143475917277	0.0	0.0	0.0	0.0
14	0.05921379003287269	0.0	0.0	0.0	0.0
15	0.07164416580313222	0.0	0.0	0.0	0.0
16	0.08588259623088405	0.0	0.0	0.0	0.0
17	0.09887798908160994	0.0	0.0	0.0	0.0
18	0.1111953614357762	0.0	0.0	0.0	0.0
19	0.13470007198317605	0.0	0.0	0.0	0.0
20	0.1478084682499952	0.0	0.0	0.0	0.0
21	0.16069085768462782	0.0	0.0	0.0	0.0
22	0.17560730860893925	0.0	0.0	0.0	0.0
23	0.19526990300916797	0.0	0.0	0.0	0.0
24	0.21289843591971785	0.0	0.0	0.0	0.0
25	0.2281538970923091	0.0	0.0	0.0	0.0
26	0.24872051882128396	0.0	0.0	0.0	0.0
27	0.2637499731616887	0.0	0.0	0.0	0.0
28	0.29098379644016636	0.0	0.0	0.0	0.0
29	0.30296215854605285	0.0	0.0	0.0	0.0
30	0.31923465046348354	0.0	0.0	0.0	0.0
31	0.33302106722686226	0.0	0.0	0.0	0.0
32	0.3515536274661583	0.0	0.0	0.0	0.0
33	0.36477502714907073	0.0	0.0	0.0	0.0
34	0.38274257030790043	0.0	0.0	0.0	0.0
35	0.41155844141168385	0.0	0.0	0.0	0.0
36	0.43212506314065874	0.0	0.0	0.0	0.0
37	0.4476065311454365	0.0	0.0	0.0	0.0
38	0.46218397182146814	0.0	0.0	0.0	0.0
39	0.47698741932968636	0.0	0.0	0.0	0.0
40	0.4923558839183709	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCC	70	0.0	46.000004	14
GTCGATA	35	1.01921614E-7	46.000004	45
CATTCCG	35	1.01921614E-7	46.000004	34
TCGATAG	35	1.01921614E-7	46.000004	46
CACCTCG	35	1.01921614E-7	46.000004	26
GTTACGG	35	1.01921614E-7	46.000004	26
CGAGGTA	35	1.01921614E-7	46.000004	32
TGTTACG	35	1.01921614E-7	46.000004	25
TACTATC	70	0.0	46.000004	13
TGATACC	45	3.092282E-10	46.0	11
CGTATGT	25	3.416514E-5	46.0	34
CGTATGA	20	6.3106645E-4	46.0	13
ATAGGTT	45	3.092282E-10	46.0	16
TACCATT	40	5.6097633E-9	46.0	21
TTTACGC	30	1.8609135E-6	46.0	37
CGGTTTA	20	6.3106645E-4	46.0	38
ATAATAC	30	1.8609135E-6	46.0	13
TAGCGTC	55	1.8189894E-12	46.0	43
CTATACG	30	1.8609135E-6	46.0	17
CGGTTAT	20	6.3106645E-4	46.0	39
>>END_MODULE