FastQCFastQC Report
Fri 17 Jun 2016
SRR1527745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890676
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG60410.6782488806255025No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT41550.46649960254907513No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT39480.44325882812605255No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC35800.401941895818457No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA33810.3795993155760344No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC32850.3688209854088355No Hit
AACGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC27070.3039264558604925No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT25290.2839416353421446No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT22280.25014707929707325No Hit
AACGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC21810.2448701884860488No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT20360.22859041896267554No Hit
AACGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC19030.21365794071020214No Hit
AACGCCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT18110.20332870763330324No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT17910.2010832221818035No Hit
AACGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA16950.19030489201460465No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC16510.18536482402130516No Hit
AACGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG16300.1830070642972304No Hit
AACGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG14500.16279769523373258No Hit
AACGCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC13570.1523561878842587No Hit
AACGCCGGGCCTGTGTTACAATTTTTCGTTTGGTCTTAGGTCTTCCCATGGC13510.15168254224880878No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG13290.14921250825215904No Hit
AACGCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA13020.14618110289263436No Hit
AACGCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA12970.14561973152975943No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC12950.14539518298460943No Hit
AACGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG11820.13270819018363578No Hit
AACGCCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG11600.13023815618698606No Hit
AACGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11400.1279926707354863No Hit
AACGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT10920.1226035056518869No Hit
AACGCCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT10110.11350928957331285No Hit
AACGCCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT9780.10980423857833825No Hit
AACGCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9760.10957969003318828No Hit
AACGCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG9650.1083446730348634No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT9320.10463962203988879No Hit
AACGCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC9010.10115911959006417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC551.8189894E-1246.011
ACACGTA206.3106866E-446.030
TTAATCG206.3106866E-446.036
ATAGGTT253.416533E-546.016
TTAGCCA253.416533E-546.034
CGTGCAA206.3106866E-446.011
ATAATCG206.3106866E-446.018
ATAATAC206.3106866E-446.013
CTATAGC301.860928E-646.035
CTATACG501.6370905E-1146.017
TTAGACG206.3106866E-446.044
CGCATAG206.3106866E-446.023
TATATCA206.3106866E-446.040
CGTCTAA206.3106866E-446.043
ATATGCG206.3106866E-446.037
TGCGGTA301.860928E-646.015
CGATTCA206.3106866E-446.041
TACGGCA206.3106866E-446.045
GCGCATA206.3106866E-446.022
CGTTCAT206.3106866E-446.044
Produced by FastQC (version 0.11.5)