##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527745_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	890676
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900635023285684	31.0	31.0	33.0	31.0	34.0
2	32.61603882893443	34.0	31.0	34.0	31.0	34.0
3	32.38363445293238	34.0	31.0	34.0	31.0	34.0
4	35.998066637026255	37.0	35.0	37.0	35.0	37.0
5	36.07067553184323	37.0	37.0	37.0	35.0	37.0
6	36.249404946355355	37.0	37.0	37.0	35.0	37.0
7	36.33888529611217	37.0	35.0	37.0	35.0	37.0
8	36.51585986374394	37.0	37.0	37.0	35.0	37.0
9	38.58671391168057	39.0	39.0	39.0	37.0	39.0
10	37.88624932074065	39.0	38.0	39.0	35.0	39.0
11	37.572398941927254	39.0	37.0	39.0	35.0	39.0
12	36.98407389443524	39.0	37.0	39.0	33.0	39.0
13	36.70674858197594	39.0	37.0	39.0	33.0	39.0
14	37.590348229883816	40.0	37.0	40.0	33.0	40.0
15	37.657938464716686	40.0	37.0	40.0	33.0	40.0
16	37.6893977158922	40.0	37.0	40.0	33.0	40.0
17	37.6655169781155	40.0	37.0	40.0	33.0	40.0
18	37.65116720333769	40.0	37.0	40.0	33.0	40.0
19	37.531559175278105	40.0	36.0	40.0	33.0	40.0
20	37.46771104195016	40.0	36.0	40.0	33.0	40.0
21	37.49704157291765	40.0	36.0	40.0	33.0	40.0
22	37.383867983419336	40.0	36.0	40.0	33.0	40.0
23	37.318110064714894	40.0	35.0	40.0	33.0	40.0
24	37.23723890617913	39.0	35.0	40.0	33.0	40.0
25	37.158769294333744	39.0	35.0	40.0	33.0	40.0
26	36.939959087255076	39.0	35.0	40.0	32.0	40.0
27	36.7723807534951	39.0	35.0	40.0	32.0	40.0
28	36.57606245144138	38.0	35.0	40.0	31.0	40.0
29	36.422318553548095	38.0	35.0	40.0	31.0	40.0
30	36.21397792238704	38.0	35.0	40.0	31.0	40.0
31	36.03473541444925	38.0	35.0	40.0	31.0	40.0
32	35.839189559390846	37.0	35.0	40.0	30.0	40.0
33	35.34938406334065	37.0	34.0	40.0	29.0	40.0
34	35.18236373271537	37.0	34.0	40.0	28.0	40.0
35	35.1111021291693	36.0	34.0	40.0	29.0	40.0
36	34.89990411777122	36.0	34.0	40.0	28.0	40.0
37	34.80601251184493	36.0	34.0	39.0	28.0	40.0
38	34.43811554369939	35.0	33.0	39.0	27.0	40.0
39	34.43871059734404	35.0	33.0	39.0	27.0	40.0
40	34.17014829185922	35.0	33.0	39.0	26.0	40.0
41	33.84294288832303	35.0	33.0	39.0	26.0	40.0
42	33.86866716965541	35.0	33.0	38.0	26.0	40.0
43	33.790953163664454	35.0	33.0	38.0	26.0	40.0
44	33.5752260081107	35.0	33.0	38.0	26.0	40.0
45	33.465900057933524	35.0	33.0	37.0	26.0	40.0
46	33.1648893649318	35.0	32.0	37.0	24.0	40.0
47	32.83502979759194	35.0	32.0	36.0	23.0	40.0
48	32.78210707372827	35.0	32.0	36.0	23.0	39.0
49	32.709253420997086	35.0	32.0	36.0	24.0	39.0
50	32.532117178412804	35.0	31.0	36.0	24.0	39.0
51	32.89481584773812	35.0	32.0	36.0	24.0	39.0
52	32.71600559574974	35.0	32.0	35.0	24.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	3.0
15	10.0
16	38.0
17	99.0
18	209.0
19	397.0
20	711.0
21	1301.0
22	2110.0
23	3123.0
24	4383.0
25	6047.0
26	7993.0
27	10263.0
28	13046.0
29	16297.0
30	20558.0
31	25684.0
32	34123.0
33	49067.0
34	87975.0
35	106263.0
36	149546.0
37	161219.0
38	155339.0
39	34869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.23137032995163	0.37791520148740954	0.15651033596953326	0.23420413259142495
8	99.0645307609052	0.6292972977828076	0.12327715128733681	0.18289479002465542
9	96.82084169776664	1.4046634241856748	0.7307932401905968	1.0437016378570882
10	59.083886845497126	26.95840013652552	5.693989733640516	8.263723284336841
11	38.308430899676196	23.150169085054497	20.452106040804964	18.08929397446434
12	31.616322882844045	16.773551774158054	25.506918340676073	26.10320700232183
13	25.778509806034965	16.03130655816481	29.437640623526402	28.752543012273822
14	26.267801085916766	17.782897484607197	29.521734053685066	26.42756737579097
15	26.89721065797215	17.897304968361112	27.257274250120133	27.94821012354661
16	31.36875811181619	17.967813211538203	25.021556660334397	25.641872016311208
17	32.06047990515069	18.70567973090102	22.716902667187618	26.51693769676066
18	32.18027655398821	19.609038528039378	22.404331092338854	25.806353825633565
19	31.655731152517863	19.76420157273801	22.003624213518723	26.576443061225408
20	30.280259039201685	21.53005133179742	21.824995845851916	26.36469378314898
21	30.346725408566076	20.62545751766074	24.244506419842907	24.783310653930275
22	30.90001302381562	18.86095504987223	24.075645913890124	26.163386012422023
23	30.739685362578534	20.672388163597088	22.883854510506627	25.704071963317748
24	30.235910701534564	20.371829935913848	22.664582856167677	26.72767650638391
25	30.062559224678782	19.675168074586043	22.027763182122342	28.23450951861283
26	28.270549560109398	19.67348396049742	22.174505656377853	29.881460823015328
27	26.822885089527503	18.55927407946324	23.499791169853008	31.118049661156245
28	29.052203045776466	20.997534456974254	22.01956716022437	27.930695337024915
29	30.232430199084742	21.05108928499252	22.224692256218873	26.491788259703863
30	30.31394132097418	19.23999299408539	23.54571134733618	26.90035433760425
31	32.724020856068876	20.484553305579134	21.036044532467475	25.755381305884516
32	34.440694483740444	19.763864749920284	21.588433953536416	24.20700681280286
33	34.38253641054659	19.605108928499252	22.251862630182018	23.76049203077213
34	30.24567856324859	22.79706649780616	24.918152055292833	22.03910288365242
35	29.076342014380092	25.124175345467936	24.694726252868605	21.104756387283366
36	30.690621505463266	24.185786975286184	21.088701166305142	24.034890352945403
37	30.875424958121695	25.411597483259907	20.546191881222803	23.166785677395595
38	32.57177694245719	24.13694766671607	20.00727537286286	23.284000017963884
39	32.18824802734103	21.896177734664455	19.76622250964436	26.14935172835015
40	28.77656970660487	23.3611324432229	21.761560881847046	26.100736968325183
41	28.031405359524676	22.59676919553238	22.01485164077622	27.356973804166724
42	26.714989513582942	20.512958696540608	22.615743547597557	30.1563082422789
43	26.142053900632778	19.10200791309073	24.971819157583678	29.784119028692814
44	27.706371340420084	19.73691892450229	23.99559435754416	28.56111537753347
45	28.213514229641305	20.41067683422479	23.204846655798516	28.170962280335388
46	27.685600599993716	21.120474785443864	23.519326893281058	27.674597721281362
47	27.597128473204624	21.29775586183977	23.68055274869874	27.424562916256868
48	29.11125931315091	21.737871010333723	25.031099973503274	24.119769703012096
49	28.124817554307064	20.896487611656763	24.68147788870476	26.29721694533141
50	26.286663163709363	21.90504740219788	24.805316411354973	27.002973022737788
51	26.73497433410129	22.064925966344664	23.906224036574468	27.293875662979577
52	27.694807090344863	21.136193183604362	25.20770740426373	25.96129232178705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.0
8	8.0
9	11.0
10	14.0
11	43.0
12	72.0
13	213.5
14	815.5
15	1276.0
16	1520.0
17	1764.0
18	1741.5
19	1719.0
20	1441.5
21	1164.0
22	1372.0
23	1580.0
24	1531.5
25	1483.0
26	1894.5
27	2306.0
28	2442.5
29	2579.0
30	2956.0
31	3333.0
32	4064.0
33	4795.0
34	4843.5
35	4892.0
36	5939.5
37	6987.0
38	8831.5
39	10518.0
40	10360.0
41	11124.0
42	11888.0
43	14677.5
44	17467.0
45	18230.0
46	18993.0
47	20133.5
48	21274.0
49	24082.0
50	26890.0
51	27018.5
52	27147.0
53	32327.0
54	37507.0
55	39178.0
56	40849.0
57	48053.0
58	55257.0
59	66139.5
60	77022.0
61	87346.5
62	97671.0
63	92717.0
64	86988.5
65	86214.0
66	77934.0
67	69654.0
68	61305.0
69	52956.0
70	41665.5
71	30375.0
72	27774.5
73	25174.0
74	23251.0
75	21328.0
76	18815.0
77	16302.0
78	12755.5
79	9209.0
80	6028.0
81	2847.0
82	1832.0
83	817.0
84	666.5
85	516.0
86	326.0
87	136.0
88	83.5
89	24.5
90	18.0
91	11.0
92	4.0
93	2.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	890676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.022584248535708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.53163739336554	9.836171113849526
2	9.450160620284043	2.461310256796868
3	3.486942704609762	1.3622701542179274
4	1.6268447937476245	0.8474289354348054
5	0.8628347053360017	0.5618168821399283
6	0.5376779525395199	0.4201173861315648
7	0.33957528965444395	0.3095503472772138
8	0.23667230291381885	0.24656680031921352
9	0.1972267068063591	0.23115612649023554
>10	3.376005904922109	12.262103691399712
>50	2.6882851116479074	25.22482242504005
>100	1.5855308361840448	34.54264545223834
>500	0.05573796882204592	4.824350024820177
>1k	0.02401020195411209	6.195094355051826
>5k	8.575072126468604E-4	0.6745960487926183
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6041	0.6782488806255025	No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4155	0.46649960254907513	No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3948	0.44325882812605255	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3580	0.401941895818457	No Hit
AACGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3381	0.3795993155760344	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3285	0.3688209854088355	No Hit
AACGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2707	0.3039264558604925	No Hit
AACGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2529	0.2839416353421446	No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	2228	0.25014707929707325	No Hit
AACGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2181	0.2448701884860488	No Hit
AACGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2036	0.22859041896267554	No Hit
AACGCCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1903	0.21365794071020214	No Hit
AACGCCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1811	0.20332870763330324	No Hit
AACGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1791	0.2010832221818035	No Hit
AACGCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1695	0.19030489201460465	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCGCCCGACTGCTC	1651	0.18536482402130516	No Hit
AACGCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	1630	0.1830070642972304	No Hit
AACGCCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1450	0.16279769523373258	No Hit
AACGCCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1357	0.1523561878842587	No Hit
AACGCCGGGCCTGTGTTACAATTTTTCGTTTGGTCTTAGGTCTTCCCATGGC	1351	0.15168254224880878	No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1329	0.14921250825215904	No Hit
AACGCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1302	0.14618110289263436	No Hit
AACGCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1297	0.14561973152975943	No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1295	0.14539518298460943	No Hit
AACGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1182	0.13270819018363578	No Hit
AACGCCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1160	0.13023815618698606	No Hit
AACGCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1140	0.1279926707354863	No Hit
AACGCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1092	0.1226035056518869	No Hit
AACGCCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1011	0.11350928957331285	No Hit
AACGCCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	978	0.10980423857833825	No Hit
AACGCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	976	0.10957969003318828	No Hit
AACGCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	965	0.1083446730348634	No Hit
AACGCCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	932	0.10463962203988879	No Hit
AACGCCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	901	0.10115911959006417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.004603245175574508	0.0	0.0	0.0	0.0
8	0.007746924807674171	0.0	0.0	0.0	0.0
9	0.008645118988274076	0.0	0.0	0.0	0.0
10	0.017963883611998077	0.0	0.0	0.0	0.0
11	0.02492488851164733	0.0	0.0	0.0	0.0
12	0.032559539046746515	0.0	0.0	0.0	0.0
13	0.042776497851070426	0.0	0.0	0.0	0.0
14	0.05523894210689409	0.0	0.0	0.0	0.0
15	0.06848730627074268	0.0	0.0	0.0	0.0
16	0.08633891561016577	0.0	0.0	0.0	0.0
17	0.1009345710449142	0.0	0.0	0.0	0.0
18	0.1131724667555879	0.0	0.0	0.0	0.0
19	0.13708688681406034	0.0	0.0	0.0	0.0
20	0.15112117088593383	0.0	0.0	0.0	0.0
21	0.16515545495780734	0.0	0.0	0.0	0.0
22	0.17975111039255576	0.0	0.0	0.0	0.0
23	0.2007463993640785	0.0	0.0	0.0	0.0
24	0.21713844316002676	0.0	0.0	0.0	0.0
25	0.2318463728673502	0.0	0.0	0.0	0.0
26	0.252504839021148	0.0	0.0	0.0	0.0
27	0.2664268488204465	0.0	0.0	0.0	0.0
28	0.29573043396251836	0.0	0.0	0.0	0.0
29	0.3068455869474422	0.0	0.0	0.0	0.0
30	0.3251462933771652	0.0	0.0	0.0	0.0
31	0.33783328617813885	0.0	0.0	0.0	0.0
32	0.3570321867884618	0.0	0.0	0.0	0.0
33	0.3700560024071604	0.0	0.0	0.0	0.0
34	0.38925490301748333	0.0	0.0	0.0	0.0
35	0.41900758524985515	0.0	0.0	0.0	0.0
36	0.4371960174070032	0.0	0.0	0.0	0.0
37	0.45190394711432663	0.0	0.0	0.0	0.0
38	0.46784689381997496	0.0	0.0	0.0	0.0
39	0.486147600249698	0.0	0.0	0.0	0.0
40	0.49658910759917185	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACC	55	1.8189894E-12	46.0	11
ACACGTA	20	6.3106866E-4	46.0	30
TTAATCG	20	6.3106866E-4	46.0	36
ATAGGTT	25	3.416533E-5	46.0	16
TTAGCCA	25	3.416533E-5	46.0	34
CGTGCAA	20	6.3106866E-4	46.0	11
ATAATCG	20	6.3106866E-4	46.0	18
ATAATAC	20	6.3106866E-4	46.0	13
CTATAGC	30	1.860928E-6	46.0	35
CTATACG	50	1.6370905E-11	46.0	17
TTAGACG	20	6.3106866E-4	46.0	44
CGCATAG	20	6.3106866E-4	46.0	23
TATATCA	20	6.3106866E-4	46.0	40
CGTCTAA	20	6.3106866E-4	46.0	43
ATATGCG	20	6.3106866E-4	46.0	37
TGCGGTA	30	1.860928E-6	46.0	15
CGATTCA	20	6.3106866E-4	46.0	41
TACGGCA	20	6.3106866E-4	46.0	45
GCGCATA	20	6.3106866E-4	46.0	22
CGTTCAT	20	6.3106866E-4	46.0	44
>>END_MODULE