FastQCFastQC Report
Fri 17 Jun 2016
SRR1527744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738043
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24240.32843614802931537No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC23380.3167837104342159No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC23290.31556426929054265No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21260.28805909682769165No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT15100.20459512521628143No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC14710.19931088026036423No Hit
AAAACGGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC13000.17614149853057343No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC12450.16868935820812608No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC12370.16760541052486103No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG11970.16218567210853568No Hit
AAAACGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA11480.15554649254853714Illumina Single End Adapter 2 (97% over 34bp)
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT11220.15202366257792568No Hit
AAAACGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT9240.12519595741711526No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA9220.124924970496299No Hit
AAAACGGGGGGGCCAGGTGGTGGTGGCGCACGCCTTTAAAAACCCAGCAC9210.12478947703589087No Hit
AAAACGGGGTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGG8950.1212666470652794No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG8910.12072467322364687No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG8770.118827764777933No Hit
AAAACGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT8770.118827764777933No Hit
AAAACGGGGCAGGGTGTCACTGGAGAGTCGTTCCTGTAAATGGCTTAACC8580.11625338903017847No Hit
AAAACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA8510.11530493480732151No Hit
AAAACGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT8420.11408549366364831No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG7820.10595588603916031No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG7640.10351700375181391No Hit
AAAACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCC7470.10121361492487564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA207.854649E-444.023
TAATAGA302.5264817E-644.026
GGTAATC254.4412875E-544.08
TTAATAG408.305506E-944.025
GCGAATT207.854649E-444.037
CCGATAT207.854649E-444.017
CTATACT254.4412875E-544.030
TTATGCG254.4412875E-544.037
CCGGATA207.854649E-444.013
ACCGATA207.854649E-444.016
CAATTAT207.854649E-444.037
CCGCTTA207.854649E-444.021
TGCGGAT408.305506E-944.024
GCGTTAG207.854649E-444.024
TCGATAT207.854649E-444.037
CGTTAGG207.854649E-444.025
TCAATTA207.854649E-444.036
AATGCGA302.5264817E-644.027
ATATCGA207.854649E-444.040
GTAGCAA254.4412875E-544.08
Produced by FastQC (version 0.11.5)