##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527744_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	738043
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.90987652480953	33.0	31.0	34.0	3.0	34.0
2	30.53641183508278	34.0	31.0	34.0	16.0	34.0
3	32.50744197831292	34.0	31.0	34.0	28.0	34.0
4	36.39125498107834	37.0	35.0	37.0	35.0	37.0
5	36.33101594351549	37.0	37.0	37.0	35.0	37.0
6	36.34546767600262	37.0	37.0	37.0	35.0	37.0
7	36.62871404511661	37.0	37.0	37.0	35.0	37.0
8	36.69326583952426	37.0	37.0	37.0	35.0	37.0
9	38.594776998088186	39.0	39.0	39.0	38.0	39.0
10	38.17735687487043	39.0	39.0	39.0	37.0	39.0
11	37.30796579603086	39.0	35.0	39.0	35.0	39.0
12	37.120463170845056	39.0	37.0	39.0	34.0	39.0
13	37.09294580397077	39.0	37.0	39.0	33.0	39.0
14	37.951516375062155	40.0	37.0	40.0	34.0	40.0
15	38.016745636771844	40.0	37.0	40.0	34.0	40.0
16	38.04802701197627	40.0	37.0	40.0	35.0	40.0
17	38.0095265452013	40.0	37.0	40.0	34.0	40.0
18	37.9289214856045	40.0	37.0	40.0	34.0	40.0
19	37.914586277493314	40.0	37.0	40.0	34.0	40.0
20	37.843328911730076	40.0	36.0	40.0	34.0	40.0
21	37.76758129268891	40.0	36.0	40.0	34.0	40.0
22	37.75528390622227	40.0	36.0	40.0	34.0	40.0
23	37.59205222459938	40.0	36.0	40.0	34.0	40.0
24	37.41874389432594	40.0	35.0	40.0	33.0	40.0
25	37.33440328002569	40.0	35.0	40.0	33.0	40.0
26	37.16401618875865	39.0	35.0	40.0	33.0	40.0
27	37.035351598755085	39.0	35.0	40.0	33.0	40.0
28	36.78717364706392	39.0	35.0	40.0	33.0	40.0
29	36.706721695077384	39.0	35.0	40.0	33.0	40.0
30	36.789821189280296	39.0	35.0	40.0	33.0	40.0
31	36.74568690442156	39.0	35.0	40.0	33.0	40.0
32	36.688402979230204	39.0	35.0	40.0	33.0	40.0
33	36.527444877872966	39.0	35.0	40.0	33.0	40.0
34	36.154851411096644	39.0	35.0	40.0	31.0	40.0
35	36.00714728003653	38.0	35.0	40.0	31.0	40.0
36	35.88470861453872	38.0	35.0	40.0	31.0	40.0
37	35.65070463374085	37.0	35.0	40.0	31.0	40.0
38	35.42051208398426	37.0	35.0	40.0	30.0	40.0
39	35.23432510029903	37.0	35.0	40.0	30.0	40.0
40	34.97813677522854	36.0	35.0	40.0	29.0	40.0
41	34.754842197541336	36.0	35.0	40.0	27.0	40.0
42	34.63545890957573	36.0	35.0	40.0	27.0	40.0
43	34.50883620602052	35.0	34.0	40.0	27.0	40.0
44	34.174675459288956	35.0	34.0	40.0	24.0	40.0
45	34.13084738965074	35.0	34.0	40.0	24.0	40.0
46	33.893199989702495	35.0	34.0	39.0	23.0	40.0
47	33.86391036836607	35.0	34.0	39.0	23.0	40.0
48	33.70749942754013	35.0	34.0	39.0	23.0	40.0
49	33.631839879248226	35.0	34.0	39.0	24.0	40.0
50	33.17306986178312	35.0	33.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	13.0
16	34.0
17	86.0
18	151.0
19	325.0
20	543.0
21	949.0
22	1590.0
23	2591.0
24	3763.0
25	4974.0
26	6813.0
27	9043.0
28	11493.0
29	12732.0
30	13390.0
31	15310.0
32	20311.0
33	33572.0
34	64466.0
35	74319.0
36	84839.0
37	132481.0
38	159162.0
39	85087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.10804655013325	0.5654142102831407	0.2035111775330164	0.12302806205058514
8	98.57596373111052	0.8616029147353204	0.2682770516081041	0.2941563025460576
9	91.52664546645656	4.446895370594938	2.551341859485152	1.4751173034633482
10	47.25469925194061	37.38061332469788	6.834290142986249	8.530397280375263
11	32.9463188459209	25.83264118757308	22.020803665911064	19.200236300594952
12	28.682881620718575	21.655648790111144	25.578184468926608	24.083285120243673
13	22.261711038516726	21.837074533597637	28.207435068146435	27.693779359739203
14	24.572822992698256	23.452969542425034	27.75149957387307	24.22270789100364
15	24.83459635820677	24.13409516789672	26.28207299574686	24.749235478149647
16	29.611960278737147	24.85586883149085	23.250542312575284	22.28162857719672
17	28.25526425967051	25.872069784551847	21.67055307075604	24.202112885021602
18	26.437754981755806	26.052953554196705	22.50681870839504	25.002472755652448
19	26.43219974987907	26.149695884928114	22.281357590275906	25.136746774916908
20	28.032648504219942	25.80283262628329	21.752933094684185	24.41158577481258
21	28.178032987237874	23.926790173472277	24.19018946050569	23.70498737878416
22	27.86517858715549	23.4357618729532	23.364898793159746	25.33416074673156
23	26.778792021603078	25.106667226706303	22.161445877814707	25.953094873875916
24	27.717355221850216	26.001872519622843	22.450453428865256	23.830318829661685
25	27.435664317661708	26.32556639653787	20.77412833669583	25.464640949104588
26	27.13148149904545	26.20809356636402	21.761604676150306	24.898820258440228
27	27.53565849144291	24.931067702017362	20.81396341405582	26.71931039248391
28	27.180530131713194	26.363233578531332	21.238870905895727	25.217365383859747
29	28.813632810012425	24.75411324272434	21.459860739821394	24.972393207441844
30	27.057637563123016	25.667745646256385	22.44936948118199	24.825247309438613
31	28.827317649513645	25.189182744094857	21.01503570930149	24.968463897090007
32	29.11212490329154	24.394378105340746	22.067819896672685	24.425677094695025
33	29.719542086301203	24.982148736591228	21.585192190698915	23.71311698640865
34	28.00771770750485	26.162703257127294	23.529252360634814	22.300326674733046
35	27.353961761035606	26.73611158157452	22.961128281143512	22.948798376246373
36	28.6515826313643	26.85331342482755	21.362440941787945	23.132663002020205
37	30.165993038346006	25.596476086081704	20.989156458363535	23.248374417208755
38	29.00725296493565	26.7558936267941	21.572320311960144	22.66453309631011
39	28.616354331658183	25.077129652337334	22.416173583381998	23.89034243262249
40	28.304854866179884	24.749506465070464	22.291655093266925	24.65398357548273
41	26.087639880061186	25.74890622904086	22.9893109209084	25.174142969989553
42	26.9340675272308	23.961341005876353	22.79135497525212	26.31323649164073
43	27.421437504318853	23.30026841254507	23.474919483011153	25.803374600124922
44	25.748635242120038	23.918525072387382	23.897794572944935	26.435045112547645
45	27.101130963914027	24.640705216362733	22.885929410616995	25.37223440910624
46	27.45856271247068	24.859120674540645	22.871431610353326	24.810885002635345
47	26.903446005178562	24.90288506225247	23.047573108883903	25.146095823685073
48	26.991381260983438	24.31281104217505	24.54287893794806	24.152928758893452
49	27.946068183019147	24.27649879478567	23.761894632155578	24.015538390039602
50	25.277117999899733	24.093447129774283	24.612251589677026	26.017183280648958
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	10.0
4	20.0
5	40.0
6	60.0
7	452.0
8	844.0
9	1912.5
10	2981.0
11	2704.0
12	2427.0
13	2687.0
14	2947.0
15	2835.5
16	2724.0
17	2890.0
18	3056.0
19	3131.5
20	3207.0
21	3965.0
22	4723.0
23	5287.0
24	5851.0
25	6584.5
26	7318.0
27	7897.5
28	8477.0
29	9855.5
30	11234.0
31	11198.0
32	11162.0
33	11755.0
34	12348.0
35	13616.0
36	14884.0
37	15557.5
38	16231.0
39	16900.0
40	17569.0
41	20583.0
42	23597.0
43	23960.0
44	24323.0
45	27752.0
46	31181.0
47	31402.5
48	31624.0
49	33663.0
50	35702.0
51	38996.5
52	42291.0
53	41847.0
54	41403.0
55	49155.5
56	56908.0
57	58918.0
58	60928.0
59	59147.0
60	57366.0
61	56776.0
62	56186.0
63	52046.5
64	47907.0
65	40358.5
66	32810.0
67	27412.0
68	22014.0
69	19173.0
70	16332.0
71	13399.5
72	10467.0
73	11137.5
74	11808.0
75	8139.0
76	4470.0
77	3123.5
78	1777.0
79	1053.0
80	329.0
81	323.5
82	318.0
83	215.0
84	112.0
85	112.0
86	112.0
87	62.5
88	13.0
89	7.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	738043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.623976579266591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1836518642341	10.243003121420584
2	8.960403922548005	2.4415266636312505
3	3.2288414383512194	1.3196898040278742
4	1.5888879565006655	0.865878892257753
5	0.7909437504626644	0.538789956591031
6	0.5359618749919494	0.43811592193620763
7	0.36097432235243615	0.3442533999412354
8	0.26598107962459777	0.28989759994668507
9	0.20698527624914048	0.2537966300284184
>10	4.543676788773354	17.789468135067434
>50	2.9237920181279238	27.84338391216196
>100	1.359903264245546	31.477729857356852
>500	0.03799729708921379	3.5051998339404915
>1k	0.011999146449225406	2.6492662716922166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2424	0.32843614802931537	No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2338	0.3167837104342159	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2329	0.31556426929054265	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2126	0.28805909682769165	No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1510	0.20459512521628143	No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1471	0.19931088026036423	No Hit
AAAACGGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC	1300	0.17614149853057343	No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1245	0.16868935820812608	No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1237	0.16760541052486103	No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1197	0.16218567210853568	No Hit
AAAACGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1148	0.15554649254853714	Illumina Single End Adapter 2 (97% over 34bp)
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1122	0.15202366257792568	No Hit
AAAACGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	924	0.12519595741711526	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	922	0.124924970496299	No Hit
AAAACGGGGGGGCCAGGTGGTGGTGGCGCACGCCTTTAAAAACCCAGCAC	921	0.12478947703589087	No Hit
AAAACGGGGTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGG	895	0.1212666470652794	No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	891	0.12072467322364687	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	877	0.118827764777933	No Hit
AAAACGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	877	0.118827764777933	No Hit
AAAACGGGGCAGGGTGTCACTGGAGAGTCGTTCCTGTAAATGGCTTAACC	858	0.11625338903017847	No Hit
AAAACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	851	0.11530493480732151	No Hit
AAAACGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	842	0.11408549366364831	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	782	0.10595588603916031	No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	764	0.10351700375181391	No Hit
AAAACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCC	747	0.10121361492487564	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.05528133184651843	0.0	0.0	0.0	0.0
8	0.08712229504242977	0.0	0.0	0.0	0.0
9	0.16950231897057488	0.0	0.0	0.0	0.0
10	0.25784405515667785	0.0	0.0	0.0	0.0
11	0.30973805049299297	0.0	0.0	0.0	0.0
12	0.3659678365623683	0.0	0.0	0.0	0.0
13	0.3952344240105251	0.0	0.0	0.0	0.0
14	0.4361534490537814	0.0	0.0	0.0	0.0
15	0.480459810607241	0.0	0.0	0.0	0.0
16	0.5242241983190681	0.0	0.0	0.0	0.0
17	0.5666336514268139	0.0	0.0	0.0	0.0
18	0.6049783007223156	0.0	0.0	0.0	0.0
19	0.6496911426569997	0.0	0.0	0.0	0.0
20	0.696707373418622	0.0	0.0	0.0	0.0
21	0.740471761130449	0.0	0.0	0.0	0.0
22	0.7869460180504388	0.0	0.0	0.0	0.0
23	0.8391110003075701	0.0	0.0	0.0	0.0
24	0.8935793713916398	0.0	0.0	0.0	0.0
25	0.9481832359361175	0.0	0.0	0.0	0.0
26	1.002516113559779	0.0	0.0	0.0	0.0
27	1.0491258639401768	0.0	0.0	0.0	0.0
28	1.1007488723556758	0.0	0.0	0.0	0.0
29	1.1363836524430149	0.0	0.0	0.0	0.0
30	1.1726958998323946	0.0	0.0	0.0	0.0
31	1.1992526180723888	0.0	0.0	0.0	0.0
32	1.225944829772791	0.0	0.0	0.0	0.0
33	1.2518240807107446	0.0	0.0	0.0	0.0
34	1.2770258643466572	0.0	0.0	0.0	0.0
35	1.309273307923793	0.0	0.0	0.0	0.0
36	1.3329846634952165	0.0	0.0	0.0	0.0
37	1.3577799667499049	0.0	0.0	0.0	0.0
38	1.3779684923507167	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	20	7.854649E-4	44.0	23
TAATAGA	30	2.5264817E-6	44.0	26
GGTAATC	25	4.4412875E-5	44.0	8
TTAATAG	40	8.305506E-9	44.0	25
GCGAATT	20	7.854649E-4	44.0	37
CCGATAT	20	7.854649E-4	44.0	17
CTATACT	25	4.4412875E-5	44.0	30
TTATGCG	25	4.4412875E-5	44.0	37
CCGGATA	20	7.854649E-4	44.0	13
ACCGATA	20	7.854649E-4	44.0	16
CAATTAT	20	7.854649E-4	44.0	37
CCGCTTA	20	7.854649E-4	44.0	21
TGCGGAT	40	8.305506E-9	44.0	24
GCGTTAG	20	7.854649E-4	44.0	24
TCGATAT	20	7.854649E-4	44.0	37
CGTTAGG	20	7.854649E-4	44.0	25
TCAATTA	20	7.854649E-4	44.0	36
AATGCGA	30	2.5264817E-6	44.0	27
ATATCGA	20	7.854649E-4	44.0	40
GTAGCAA	25	4.4412875E-5	44.0	8
>>END_MODULE