FastQCFastQC Report
Fri 17 Jun 2016
SRR1527743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934379
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC31720.339476807590924No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC29260.3131491611005813No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT28090.3006274755746865No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25010.2676644059851516No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG20550.21993216885225372No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19080.20419979472997576No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG16100.17230695467256862No Hit
AAAACGGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT16060.1718788628597175No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG15810.16920328902939813No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT15230.16299595774305714No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT14030.1501532033575241No Hit
AAAACGGGGGGGCCAGGTGGTGGTGGCGCACGCCTTTAAAAACCCAGCACTT12590.1347418980948844No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11670.12489578639930905No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG11660.12478876344609628No Hit
AAAACGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC11420.12222021256898968No Hit
AAAACGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA11320.12114998303686192No Hit
AAAACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG10960.117297156721202No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC10900.11665501900192535No Hit
AAAACGGGGTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG10640.1138724222183932No Hit
AAAACGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC10280.11001959590273326No Hit
AAAACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG9940.1063808154934989No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9820.10509654005494559No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC9800.10488249414852004No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC9530.1019928744117751No Hit
AAAACGGGGCAGGGTGTCACTGGAGAGTCGTTCCTGTAAATGGCTTAACCTT9420.10081562192643456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAA1050.046.00000442
TGATCGT301.8610262E-646.09
AGGTATA405.6097633E-946.036
AACGTAC206.310846E-446.028
CTATGCG206.310846E-446.014
TAATAGG206.310846E-446.014
GGTACGA206.310846E-446.06
CTCAATT501.6370905E-1146.031
AGTCAAT501.6370905E-1146.014
TTTACGG206.310846E-446.020
CCGATTA800.046.040
ACGTTAG206.310846E-446.030
TTTCGAA301.8610262E-646.042
TTATGCG253.416661E-546.037
CGCATAT206.310846E-446.042
CAACCGA206.310846E-446.028
TTCCGTA253.416661E-546.044
CGACATA206.310846E-446.046
CGATTAG800.046.041
CCGCTTA301.8610262E-646.021
Produced by FastQC (version 0.11.5)