##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527743_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	934379
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16795647162447	33.0	31.0	34.0	31.0	34.0
2	32.816721052164056	34.0	31.0	34.0	31.0	34.0
3	33.11072380693487	34.0	31.0	34.0	31.0	34.0
4	36.568158102868324	37.0	37.0	37.0	35.0	37.0
5	36.24730864028408	37.0	37.0	37.0	35.0	37.0
6	36.102623239606196	37.0	37.0	37.0	35.0	37.0
7	36.55570277157342	37.0	37.0	37.0	35.0	37.0
8	36.6177386264032	37.0	37.0	37.0	35.0	37.0
9	38.49586088728449	39.0	39.0	39.0	37.0	39.0
10	37.864786130681445	39.0	38.0	39.0	35.0	39.0
11	36.89671535854295	39.0	35.0	39.0	34.0	39.0
12	36.694595019793894	39.0	35.0	39.0	33.0	39.0
13	36.66610336918959	39.0	35.0	39.0	33.0	39.0
14	37.555740229607046	40.0	36.0	40.0	33.0	40.0
15	37.64198574668309	40.0	36.0	40.0	33.0	40.0
16	37.67894291288653	40.0	36.0	40.0	33.0	40.0
17	37.6368828922739	40.0	36.0	40.0	33.0	40.0
18	37.59039426185734	40.0	36.0	40.0	33.0	40.0
19	37.591122017939185	40.0	36.0	40.0	33.0	40.0
20	37.564848953154986	40.0	35.0	40.0	33.0	40.0
21	37.474176966734056	40.0	35.0	40.0	33.0	40.0
22	37.446614275363636	40.0	35.0	40.0	33.0	40.0
23	37.311959065860854	40.0	35.0	40.0	33.0	40.0
24	37.12887918071789	39.0	35.0	40.0	33.0	40.0
25	37.069060841478674	39.0	35.0	40.0	33.0	40.0
26	36.878222862457314	39.0	35.0	40.0	33.0	40.0
27	36.75870283899788	39.0	35.0	40.0	32.0	40.0
28	36.543703358059204	38.0	35.0	40.0	32.0	40.0
29	36.44865627331094	38.0	35.0	40.0	32.0	40.0
30	36.23621250049498	38.0	35.0	40.0	31.0	40.0
31	36.056843101139904	38.0	35.0	40.0	31.0	40.0
32	35.912232616529266	38.0	35.0	40.0	31.0	40.0
33	35.71993912534421	38.0	35.0	40.0	30.0	40.0
34	35.49977471668349	37.0	35.0	40.0	29.0	40.0
35	35.26607832581854	37.0	34.0	40.0	29.0	40.0
36	35.15384442501383	37.0	34.0	40.0	29.0	40.0
37	34.87931235612102	36.0	34.0	40.0	28.0	40.0
38	34.64435309440816	36.0	34.0	40.0	27.0	40.0
39	34.468543278476936	36.0	34.0	40.0	26.0	40.0
40	34.203559797469765	35.0	33.0	40.0	25.0	40.0
41	33.96048070429665	35.0	33.0	39.0	23.0	40.0
42	33.84461337423037	35.0	33.0	39.0	23.0	40.0
43	33.72440626341131	35.0	33.0	39.0	23.0	40.0
44	33.62202703613844	35.0	33.0	39.0	23.0	40.0
45	33.47234687423412	35.0	33.0	39.0	23.0	40.0
46	33.285928943180444	35.0	33.0	39.0	23.0	40.0
47	33.123946492804315	35.0	33.0	38.0	23.0	40.0
48	32.87093673980259	35.0	32.0	38.0	22.0	40.0
49	33.121170317397976	35.0	33.0	38.0	23.0	40.0
50	33.164282373640674	35.0	33.0	38.0	23.0	40.0
51	33.13708677099978	35.0	33.0	38.0	23.0	40.0
52	32.66039904578335	35.0	33.0	37.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	3.0
15	17.0
16	38.0
17	131.0
18	328.0
19	643.0
20	1129.0
21	1852.0
22	3091.0
23	4473.0
24	6378.0
25	8551.0
26	11511.0
27	14909.0
28	17268.0
29	18087.0
30	19844.0
31	23562.0
32	30364.0
33	44746.0
34	93324.0
35	89595.0
36	112685.0
37	148085.0
38	203994.0
39	79769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.27920041011195	0.5102854409185138	0.12007975350473417	0.09043439546479533
8	98.68190530823145	0.8676350816959713	0.21447399823840219	0.23598561183417005
9	91.73996847103798	4.336356018275239	2.538584450207036	1.385091060479741
10	47.28969722136306	37.38697038353816	6.73281398661571	8.590518408483067
11	33.014975721843065	25.662926927938233	22.034206676305867	19.287890673912834
12	28.662138168773055	21.488068546061072	25.711408325743623	24.138384959422247
13	22.374967759335345	21.478115411412286	28.3726410803325	27.77427574891987
14	24.548925007946455	23.16404799337314	27.866850603448924	24.420176395231486
15	25.088320692138844	23.789597154901813	26.362642996043363	24.75943915691598
16	29.611859855583226	24.49198879683726	23.345344876115583	22.550806471463936
17	28.426901717611376	25.420519938911294	21.845846278651383	24.306732064825944
18	26.673758721032897	25.58372994256078	22.564719455381596	25.177791881024724
19	26.64068862849015	25.685080679253282	22.329804073079554	25.344426619177014
20	28.062381538968662	25.522298767416647	21.862862928212216	24.552456765402475
21	28.21970528019144	23.606159813095115	24.34204963938616	23.832085267327283
22	27.87594755447201	23.094911165597686	23.604982560609773	25.424158719320534
23	26.885236076581343	24.791867111739453	22.233697461094483	26.089199350584718
24	27.711453275383974	25.732277801620114	22.62540146985324	23.930867453142675
25	27.67356714994665	25.911327202345085	20.818532950761952	25.596572696946314
26	27.146586128326945	25.931768586408726	21.848307806575278	25.073337478689055
27	27.6011126106216	24.43226998894453	20.86904778467838	27.09756961575549
28	27.233488766335718	26.006042515938393	21.38147368466115	25.378995033064744
29	28.954417853997143	24.479360088358153	21.552175295035525	25.014046762609176
30	27.262599009609588	25.44053323116209	22.503395303190675	24.793472456037648
31	28.998832379580445	24.996173929422643	21.0233748832112	24.981618807785704
32	29.255901513197536	24.09140188296184	22.202767827615986	24.449928776224635
33	29.788233682477884	24.77998756393284	21.624415788454147	23.80736296513513
34	27.99955906543276	25.927915760093068	23.576621478008388	22.49590369646578
35	27.578102675680853	26.560207367674142	22.903982217066094	22.957707739578908
36	28.75471302330211	26.707899043107773	21.425460118431598	23.111927815158516
37	30.177690209219172	25.30793179213146	21.156832505867534	23.357545492781835
38	28.853281163211076	26.722775233604352	21.562556521497164	22.861387081687408
39	28.654539539094948	24.913231140682743	22.407716783018454	24.024512537203854
40	28.275036147002446	24.612710688061266	22.468291774536887	24.6439613903994
41	26.1786705394706	25.61722812691638	22.9744033202801	25.229698013332918
42	27.021583318974418	23.76326950841147	22.79535391955513	26.41979325305898
43	27.410825799809285	23.10861010360892	23.463926308275333	26.016637788306458
44	25.646980507909532	23.738012091453253	23.948098148609933	26.66690925202728
45	27.270090616334485	24.181943301379846	23.031339531389296	25.51662655089637
46	27.444752075977735	24.539078896250878	22.9358750571235	25.080293970647883
47	27.04898119499689	24.576322883968924	23.150456078315116	25.224239842719072
48	27.15150918417473	23.94103463369789	24.65327238732891	24.25418379479847
49	28.11321744174473	23.890305753875033	23.955161663521977	24.04131514085826
50	25.472426071219495	23.78799181060362	24.62416214405504	26.11541997412185
51	25.450486365810875	24.32514001277854	25.06466861947882	25.159705001931766
52	27.061823949382426	24.139241143047947	23.887309111185075	24.911625796384552
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	11.5
4	23.0
5	41.5
6	60.0
7	545.5
8	1031.0
9	2254.0
10	3477.0
11	3151.5
12	2826.0
13	3165.0
14	3274.5
15	3045.0
16	2996.5
17	2948.0
18	3144.5
19	3341.0
20	3916.5
21	4492.0
22	4855.5
23	5219.0
24	6482.5
25	7746.0
26	8018.5
27	8291.0
28	10505.5
29	12720.0
30	12965.0
31	13210.0
32	13596.0
33	13982.0
34	14618.0
35	15254.0
36	17516.5
37	19779.0
38	21028.5
39	22365.0
40	22452.0
41	25301.0
42	28150.0
43	28779.5
44	29409.0
45	32631.5
46	35854.0
47	37334.0
48	38814.0
49	42029.0
50	45244.0
51	47864.5
52	50485.0
53	52731.0
54	54977.0
55	58930.0
56	62883.0
57	70196.5
58	77510.0
59	74162.5
60	70815.0
61	70906.5
62	70998.0
63	66562.0
64	52256.5
65	42387.0
66	37678.0
67	32969.0
68	26832.5
69	20696.0
70	18330.5
71	15965.0
72	16482.5
73	17000.0
74	12444.5
75	7889.0
76	5007.5
77	2126.0
78	1852.0
79	1578.0
80	1005.0
81	432.0
82	333.5
83	235.0
84	164.5
85	94.0
86	77.0
87	60.0
88	32.5
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	934379.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.81464495496151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47042296116562	9.79941319803074
2	8.181539010387485	2.0968703520656558
3	3.458866416852528	1.3297243523581515
4	1.794241460555485	0.9197026912196048
5	0.9755833491420904	0.6250877121614071
6	0.5114527811451868	0.39324514809619215
7	0.372700303444534	0.3343215444273685
8	0.26656887476531826	0.2732788388928922
9	0.2013884280789217	0.23226490834822644
>10	3.1954719999186714	11.417165926730654
>50	2.7099674641503624	25.21566093340171
>100	1.789932256617353	39.23946026538977
>500	0.055151974293531886	4.564553342791543
>1k	0.01671271948288845	3.5592507860860816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3172	0.339476807590924	No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2926	0.3131491611005813	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2809	0.3006274755746865	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2501	0.2676644059851516	No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2055	0.21993216885225372	No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1908	0.20419979472997576	No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1610	0.17230695467256862	No Hit
AAAACGGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	1606	0.1718788628597175	No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1581	0.16920328902939813	No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1523	0.16299595774305714	No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1403	0.1501532033575241	No Hit
AAAACGGGGGGGCCAGGTGGTGGTGGCGCACGCCTTTAAAAACCCAGCACTT	1259	0.1347418980948844	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1167	0.12489578639930905	No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1166	0.12478876344609628	No Hit
AAAACGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1142	0.12222021256898968	No Hit
AAAACGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	1132	0.12114998303686192	No Hit
AAAACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1096	0.117297156721202	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1090	0.11665501900192535	No Hit
AAAACGGGGTGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG	1064	0.1138724222183932	No Hit
AAAACGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	1028	0.11001959590273326	No Hit
AAAACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	994	0.1063808154934989	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	982	0.10509654005494559	No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	980	0.10488249414852004	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	953	0.1019928744117751	No Hit
AAAACGGGGCAGGGTGTCACTGGAGAGTCGTTCCTGTAAATGGCTTAACCTT	942	0.10081562192643456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.05575895862385606	0.0	0.0	0.0	0.0
8	0.08422706417845435	0.0	0.0	0.0	0.0
9	0.15646755759707784	0.0	0.0	0.0	0.0
10	0.23652072660023396	0.0	0.0	0.0	0.0
11	0.2888549507212812	0.0	0.0	0.0	0.0
12	0.33776444033951963	0.0	0.0	0.0	0.0
13	0.366660637706969	0.0	0.0	0.0	0.0
14	0.40765042878746205	0.0	0.0	0.0	0.0
15	0.4484261739615295	0.0	0.0	0.0	0.0
16	0.4870614600713415	0.0	0.0	0.0	0.0
17	0.5232352182572596	0.0	0.0	0.0	0.0
18	0.5561982878467945	0.0	0.0	0.0	0.0
19	0.5972951018805003	0.0	0.0	0.0	0.0
20	0.6370006175224401	0.0	0.0	0.0	0.0
21	0.6769201790708053	0.0	0.0	0.0	0.0
22	0.7194082914962772	0.0	0.0	0.0	0.0
23	0.7659632761438345	0.0	0.0	0.0	0.0
24	0.8145516969024347	0.0	0.0	0.0	0.0
25	0.8608926356435664	0.0	0.0	0.0	0.0
26	0.899741967659804	0.0	0.0	0.0	0.0
27	0.9337752667814666	0.0	0.0	0.0	0.0
28	0.972196506984853	0.0	0.0	0.0	0.0
29	0.9975609468962808	0.0	0.0	0.0	0.0
30	1.0235675245269853	0.0	0.0	0.0	0.0
31	1.0422965413392211	0.0	0.0	0.0	0.0
32	1.0624168565432228	0.0	0.0	0.0	0.0
33	1.0786843454315647	0.0	0.0	0.0	0.0
34	1.1009451196998221	0.0	0.0	0.0	0.0
35	1.1268446743773137	0.0	0.0	0.0	0.0
36	1.14675094367489	0.0	0.0	0.0	0.0
37	1.1632324784696575	0.0	0.0	0.0	0.0
38	1.1799280591708503	0.0	0.0	0.0	0.0
39	1.1992992137023628	0.0	0.0	0.0	0.0
40	1.219205482999939	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAAA	105	0.0	46.000004	42
TGATCGT	30	1.8610262E-6	46.0	9
AGGTATA	40	5.6097633E-9	46.0	36
AACGTAC	20	6.310846E-4	46.0	28
CTATGCG	20	6.310846E-4	46.0	14
TAATAGG	20	6.310846E-4	46.0	14
GGTACGA	20	6.310846E-4	46.0	6
CTCAATT	50	1.6370905E-11	46.0	31
AGTCAAT	50	1.6370905E-11	46.0	14
TTTACGG	20	6.310846E-4	46.0	20
CCGATTA	80	0.0	46.0	40
ACGTTAG	20	6.310846E-4	46.0	30
TTTCGAA	30	1.8610262E-6	46.0	42
TTATGCG	25	3.416661E-5	46.0	37
CGCATAT	20	6.310846E-4	46.0	42
CAACCGA	20	6.310846E-4	46.0	28
TTCCGTA	25	3.416661E-5	46.0	44
CGACATA	20	6.310846E-4	46.0	46
CGATTAG	80	0.0	46.0	41
CCGCTTA	30	1.8610262E-6	46.0	21
>>END_MODULE