FastQCFastQC Report
Fri 17 Jun 2016
SRR1527742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944290
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC29480.31219222908216754No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT28640.30329665674739753No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC26900.2848701140539453No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23180.24547543657139226No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG19810.2097872475616601No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC17750.1879719154073431No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG15890.16827457666606657No Hit
AAAACGGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT15260.16160289741498904No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14860.15736691058890806No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT14320.15164832837369877No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT14060.14889493693674613No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG11210.11871353080091922No Hit
AAAACGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA11160.1181840324476591No Hit
AAAACGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC11060.11712503574113885No Hit
AAAACGGGGGGGCCAGGTGGTGGTGGCGCACGCCTTTAAAAACCCAGCACTT11050.11701913607048682No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC10770.11405394529223013No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC10270.10875896175962893No Hit
AAAACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG10220.1082294634063688No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC10040.10632326933463236No Hit
AAAACGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC9680.10251088119115949No Hit
AAAACGGGGCAGGGTGTCACTGGAGAGTCGTTCCTGTAAATGGCTTAACCTT9650.10219318217920342No Hit
AAAACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG9610.10176958349659533No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9560.10124008514333521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG405.6097633E-946.00000410
ATTAGTG351.0193071E-746.00000440
ATTACCG206.310878E-446.00000423
CCGGATA206.310878E-446.00000413
ACCGATA206.310878E-446.00000416
GCGCCTA206.310878E-446.00000445
CGATTAG800.046.00000441
CGCGAAT206.310878E-446.00000419
TCTACTA405.6097633E-946.00000431
ATTCACG405.6097633E-946.00000410
CTACGAA206.310878E-446.00000439
TACGAAT206.310878E-446.00000424
GTCCGTA351.0193071E-746.00000428
TACTCAC206.310878E-446.00000426
ATGCGTC206.310878E-446.00000421
TTAGTCA206.310878E-446.00000440
TAGGCGG206.310878E-446.00000431
CGGTATG206.310878E-446.00000433
AATAGGT405.6097633E-946.00000446
CGTCCCA206.310878E-446.00000439
Produced by FastQC (version 0.11.5)