##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527742_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	944290
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12788550127609	33.0	31.0	34.0	31.0	34.0
2	32.791196560378694	34.0	31.0	34.0	31.0	34.0
3	33.093984898706964	34.0	31.0	34.0	31.0	34.0
4	36.54954833790467	37.0	37.0	37.0	35.0	37.0
5	36.24879327325292	37.0	37.0	37.0	35.0	37.0
6	35.98930307426744	37.0	37.0	37.0	35.0	37.0
7	36.50081754545744	37.0	37.0	37.0	35.0	37.0
8	36.57673701934787	37.0	37.0	37.0	35.0	37.0
9	38.44718571625242	39.0	39.0	39.0	37.0	39.0
10	37.763642525071745	39.0	38.0	39.0	35.0	39.0
11	36.85845026421968	39.0	35.0	39.0	33.0	39.0
12	36.640442025225305	39.0	35.0	39.0	33.0	39.0
13	36.45084137288333	39.0	35.0	39.0	33.0	39.0
14	37.4740937635684	40.0	36.0	40.0	33.0	40.0
15	37.582393120757395	40.0	36.0	40.0	33.0	40.0
16	37.64986074193309	40.0	36.0	40.0	33.0	40.0
17	37.646280274068346	40.0	36.0	40.0	33.0	40.0
18	37.6101833123299	40.0	36.0	40.0	33.0	40.0
19	37.56171515106588	40.0	36.0	40.0	33.0	40.0
20	37.5465164303339	40.0	35.0	40.0	33.0	40.0
21	37.51406029927247	40.0	35.0	40.0	33.0	40.0
22	37.380827923625155	40.0	35.0	40.0	33.0	40.0
23	37.27602113757426	39.0	35.0	40.0	33.0	40.0
24	37.145337766999546	39.0	35.0	40.0	33.0	40.0
25	37.04173188321384	39.0	35.0	40.0	33.0	40.0
26	36.80472947929132	39.0	35.0	40.0	32.0	40.0
27	36.683602494996244	39.0	35.0	40.0	32.0	40.0
28	36.486161031039195	38.0	35.0	40.0	31.0	40.0
29	36.397252962543284	38.0	35.0	40.0	31.0	40.0
30	36.17494837391056	38.0	35.0	40.0	31.0	40.0
31	36.013742600260514	38.0	35.0	40.0	31.0	40.0
32	35.874818117315655	38.0	35.0	40.0	30.0	40.0
33	35.5143737622976	38.0	35.0	40.0	30.0	40.0
34	35.39765432229506	37.0	34.0	40.0	29.0	40.0
35	35.24889811392686	37.0	34.0	40.0	29.0	40.0
36	34.99477702824344	37.0	34.0	40.0	28.0	40.0
37	34.800301814061356	36.0	34.0	40.0	27.0	40.0
38	34.295228160840416	36.0	33.0	40.0	24.0	40.0
39	34.27794851158013	35.0	33.0	40.0	25.0	40.0
40	34.098623304281524	35.0	33.0	40.0	24.0	40.0
41	33.687846953795976	35.0	33.0	39.0	23.0	40.0
42	33.70215294030436	35.0	33.0	39.0	23.0	40.0
43	33.683516716263014	35.0	33.0	39.0	23.0	40.0
44	33.56781603109214	35.0	33.0	39.0	23.0	40.0
45	33.41404653231528	35.0	33.0	39.0	23.0	40.0
46	33.065955373878786	35.0	33.0	39.0	22.0	40.0
47	32.83474356394752	35.0	32.0	38.0	22.0	40.0
48	32.73480710374991	35.0	32.0	38.0	22.0	40.0
49	32.66932616039564	35.0	32.0	38.0	22.0	40.0
50	32.51567844624003	35.0	32.0	37.0	21.0	40.0
51	32.76684916709909	35.0	33.0	37.0	22.0	40.0
52	32.501584259072956	35.0	32.0	37.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	4.0
15	13.0
16	50.0
17	160.0
18	327.0
19	649.0
20	1105.0
21	1947.0
22	3197.0
23	4659.0
24	6632.0
25	8914.0
26	11784.0
27	15407.0
28	17990.0
29	18857.0
30	20755.0
31	24807.0
32	32848.0
33	49747.0
34	95636.0
35	91023.0
36	117664.0
37	150879.0
38	200900.0
39	68333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.25340732190323	0.5296042529307734	0.12474981202808459	0.09223861313791314
8	98.71141280750618	0.841690582342289	0.20946954854970404	0.23742706160183844
9	91.74141418420189	4.297408635059145	2.551758464031177	1.409418716707791
10	47.167607408740956	37.31163096082771	6.874053521693548	8.646708108737782
11	32.919018521852394	25.791229389276598	22.009763949634117	19.279988139236888
12	28.733334039331137	21.39268656874477	25.731713774370164	24.142265617553928
13	22.53767380783446	21.48683137595442	28.491670990903216	27.4838238253079
14	24.56597019983268	23.158563576867273	27.891325757976894	24.384140465323153
15	25.034258543455927	23.812388143472873	26.424509419775706	24.72884389329549
16	29.6808183926548	24.514079361213188	23.243601012400852	22.561501233731164
17	28.486481907041266	25.399506507534763	21.79849410668333	24.31551747874064
18	26.705673045356832	25.62263711359858	22.55144076501922	25.120249076025374
19	26.63556746338519	25.7258892924843	22.353302481229285	25.28524076290123
20	27.98684726090502	25.526692011987844	21.953425324847238	24.533035402259898
21	28.29522710184371	23.604295290641648	24.36677291933622	23.73370468817842
22	28.05314045473318	23.045992226964174	23.49098264304398	25.40988467525866
23	26.861769159897914	24.70988785224878	22.31655529551303	26.111787692340275
24	27.747831704243396	25.734361266136464	22.581092672801788	23.93671435681835
25	27.611221129102287	25.947431403488334	20.871448389795507	25.56989907761387
26	27.3242330216353	25.837507545351535	21.846890256171303	24.99136917684186
27	27.644367726016373	24.472672589988246	20.902688792637854	26.980270891357527
28	27.264082008704953	26.03776382255451	21.43186944688602	25.266284721854515
29	28.98039797096231	24.404049603405735	21.588706859121668	25.026845566510286
30	27.173325990956172	25.40766078217497	22.598672018130024	24.820341208738842
31	28.953499454616693	24.95832847959843	21.06185599762785	25.02631606815703
32	29.20691736648699	24.081161507587712	22.226540575458813	24.485380550466488
33	29.83479651378284	24.693367503627066	21.65415285558462	23.817683127005477
34	27.953594764320282	25.832742060172194	23.661375213123087	22.552287962384437
35	27.46582088129706	26.60062057207002	22.90758135742198	23.02597718921094
36	28.924800643869997	26.620847409164554	21.42191487784473	23.032437069120714
37	30.131950989632422	25.33437821008377	21.05518431837677	23.47848648190704
38	28.867720721388558	26.668184561946013	21.504198921941352	22.959895794724076
39	28.69944614472249	24.892141185440913	22.446494191403065	23.96191847843353
40	28.28876722193394	24.558027724533776	22.391108663652055	24.762096389880227
41	26.368488494000786	25.552002033273673	23.027565684270723	25.051943788454818
42	27.061072340065024	23.793114403414208	22.733164599858092	26.41264865666268
43	27.257622128795177	23.114191614864076	23.613190862976417	26.014995393364327
44	25.687024113355005	23.6805430535111	23.998453864808482	26.633978968325405
45	27.232206207838694	24.237045822787493	23.00077306759576	25.529974901778058
46	27.514746529138296	24.645818551504306	23.004161857056623	24.835273062300775
47	27.296699107265777	24.543413569983798	23.144902519353163	25.01498480339726
48	27.15881773607684	23.930148577237926	24.65492592318038	24.256107763504854
49	28.17333658092323	23.874763049486916	23.86396128308041	24.08793908650944
50	25.435830094568406	23.828908492094588	24.668375181353184	26.066886231983823
51	25.525209416598717	24.330343432631924	24.9594933759756	25.18495377479376
52	27.106079700092135	24.175941712821274	23.847335034788042	24.870643552298553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	12.5
4	25.0
5	42.0
6	59.0
7	532.5
8	1006.0
9	2152.5
10	3299.0
11	3117.0
12	2935.0
13	3199.0
14	3283.5
15	3104.0
16	3118.5
17	3133.0
18	3212.0
19	3291.0
20	3884.5
21	4478.0
22	4883.5
23	5289.0
24	6484.5
25	7680.0
26	7946.0
27	8212.0
28	10586.0
29	12960.0
30	13128.0
31	13296.0
32	13851.0
33	14406.0
34	15038.0
35	15670.0
36	17955.5
37	20241.0
38	21618.5
39	22850.5
40	22705.0
41	25545.5
42	28386.0
43	29059.5
44	29733.0
45	32851.5
46	35970.0
47	37526.0
48	39082.0
49	42373.0
50	45664.0
51	48151.0
52	50638.0
53	53288.0
54	55938.0
55	59692.0
56	63446.0
57	70813.0
58	78180.0
59	74901.0
60	71622.0
61	71555.0
62	71488.0
63	66886.5
64	52706.0
65	43127.0
66	38171.5
67	33216.0
68	27319.0
69	21422.0
70	18879.5
71	16337.0
72	16727.0
73	17117.0
74	12474.5
75	7832.0
76	5016.0
77	2200.0
78	1882.5
79	1565.0
80	993.0
81	421.0
82	318.0
83	215.0
84	145.5
85	76.0
86	77.0
87	78.0
88	39.5
89	0.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	944290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.02377253406569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70795661612928	12.611983934379662
2	8.45836887659946	2.7106995777570093
3	3.1847847157885334	1.5309679756729353
4	1.4809251240246213	0.9492002930941421
5	0.8464801685643558	0.678190283783641
6	0.45442052791669846	0.4368918704488333
7	0.3075301641409354	0.3449455378290764
8	0.225738640301747	0.2893747699475574
9	0.15221135533408336	0.2195100121477678
>10	2.6523149672784245	11.896215353149458
>50	2.1670831951994027	25.09878232182722
>100	1.312129831155536	36.04400741334116
>500	0.03737501045000509	3.917480485878618
>1k	0.012680807116966012	3.2717501707429384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2948	0.31219222908216754	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2864	0.30329665674739753	No Hit
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2690	0.2848701140539453	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2318	0.24547543657139226	No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1981	0.2097872475616601	No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1775	0.1879719154073431	No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1589	0.16827457666606657	No Hit
AAAACGGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	1526	0.16160289741498904	No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1486	0.15736691058890806	No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1432	0.15164832837369877	No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1406	0.14889493693674613	No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1121	0.11871353080091922	No Hit
AAAACGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	1116	0.1181840324476591	No Hit
AAAACGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1106	0.11712503574113885	No Hit
AAAACGGGGGGGCCAGGTGGTGGTGGCGCACGCCTTTAAAAACCCAGCACTT	1105	0.11701913607048682	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1077	0.11405394529223013	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1027	0.10875896175962893	No Hit
AAAACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1022	0.1082294634063688	No Hit
AAAACGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1004	0.10632326933463236	No Hit
AAAACGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	968	0.10251088119115949	No Hit
AAAACGGGGCAGGGTGTCACTGGAGAGTCGTTCCTGTAAATGGCTTAACCTT	965	0.10219318217920342	No Hit
AAAACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	961	0.10176958349659533	No Hit
AAAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	956	0.10124008514333521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.05210263796079594	0.0	0.0	0.0	0.0
8	0.07931885331836618	0.0	0.0	0.0	0.0
9	0.15186012771500282	0.0	0.0	0.0	0.0
10	0.23181437905728114	0.0	0.0	0.0	0.0
11	0.28169312393438456	0.0	0.0	0.0	0.0
12	0.33051287210496777	0.0	0.0	0.0	0.0
13	0.3569877897679738	0.0	0.0	0.0	0.0
14	0.39627656757987484	0.0	0.0	0.0	0.0
15	0.4385305361700325	0.0	0.0	0.0	0.0
16	0.47845471200584566	0.0	0.0	0.0	0.0
17	0.5186965868536149	0.0	0.0	0.0	0.0
18	0.5527962808035667	0.0	0.0	0.0	0.0
19	0.5880608711306908	0.0	0.0	0.0	0.0
20	0.6280909466371559	0.0	0.0	0.0	0.0
21	0.6653676307066685	0.0	0.0	0.0	0.0
22	0.7053977062131337	0.0	0.0	0.0	0.0
23	0.7535820563598047	0.0	0.0	0.0	0.0
24	0.8008133094706076	0.0	0.0	0.0	0.0
25	0.8433849770727213	0.0	0.0	0.0	0.0
26	0.881085259824842	0.0	0.0	0.0	0.0
27	0.9148672547628377	0.0	0.0	0.0	0.0
28	0.9504495441019178	0.0	0.0	0.0	0.0
29	0.973112073621451	0.0	0.0	0.0	0.0
30	0.9968335998475045	0.0	0.0	0.0	0.0
31	1.0133539484692202	0.0	0.0	0.0	0.0
32	1.0322040898452807	0.0	0.0	0.0	0.0
33	1.051054231221341	0.0	0.0	0.0	0.0
34	1.0694807739147931	0.0	0.0	0.0	0.0
35	1.0937317984941066	0.0	0.0	0.0	0.0
36	1.1118406421756029	0.0	0.0	0.0	0.0
37	1.1320674792701395	0.0	0.0	0.0	0.0
38	1.1527179150472842	0.0	0.0	0.0	0.0
39	1.1715680564233446	0.0	0.0	0.0	0.0
40	1.186817608997236	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	40	5.6097633E-9	46.000004	10
ATTAGTG	35	1.0193071E-7	46.000004	40
ATTACCG	20	6.310878E-4	46.000004	23
CCGGATA	20	6.310878E-4	46.000004	13
ACCGATA	20	6.310878E-4	46.000004	16
GCGCCTA	20	6.310878E-4	46.000004	45
CGATTAG	80	0.0	46.000004	41
CGCGAAT	20	6.310878E-4	46.000004	19
TCTACTA	40	5.6097633E-9	46.000004	31
ATTCACG	40	5.6097633E-9	46.000004	10
CTACGAA	20	6.310878E-4	46.000004	39
TACGAAT	20	6.310878E-4	46.000004	24
GTCCGTA	35	1.0193071E-7	46.000004	28
TACTCAC	20	6.310878E-4	46.000004	26
ATGCGTC	20	6.310878E-4	46.000004	21
TTAGTCA	20	6.310878E-4	46.000004	40
TAGGCGG	20	6.310878E-4	46.000004	31
CGGTATG	20	6.310878E-4	46.000004	33
AATAGGT	40	5.6097633E-9	46.000004	46
CGTCCCA	20	6.310878E-4	46.000004	39
>>END_MODULE