FastQCFastQC Report
Fri 17 Jun 2016
SRR1527741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410969
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT165041.1696926013257556No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC114890.8142631057096222No Hit
GCGAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC77090.5463621100109215No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG71900.5095788780618142No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA54070.3832118210959986No Hit
GCGAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC49870.35344504379614294No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT48490.34366453125476176No Hit
GCGAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC48320.3424596855069105No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC43730.3099288503149254No Hit
GCGAATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG41180.2918561640971559No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT36760.26053017465302214No Hit
GCGAATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC34270.24288272811096487No Hit
GCGAATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG33270.23579540018242784No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC30770.21807708036108517No Hit
GCGAATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG27230.192987939494064No Hit
GCGAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG25750.18249869415982917No Hit
GCGAATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC24980.17704145165485563No Hit
GCGAATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT22780.1614493302120741No Hit
GCGAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG21510.15244842374283205No Hit
GCGAATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG19480.13806114804790182No Hit
GCGAATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC18940.13423399096649183No Hit
GCGAATGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT16070.11389335981159046No Hit
GCGAATGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG15630.11077493552303418No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT15100.10701865172090953No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA14310.10141966265736525No Hit
GCGAATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC14170.10042743674737006No Hit
GCGAATGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA14170.10042743674737006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAC207.8570907E-444.00000416
CTCGTAA207.8570907E-444.00000416
CGACGTT207.8570907E-444.00000437
TTATAGG207.8570907E-444.00000429
AAACGTT408.314601E-944.00000427
GCCGATT207.8570907E-444.00000419
TACTCGA207.8570907E-444.00000444
CGTGTAA207.8570907E-444.00000442
ATAACTA207.8570907E-444.00000437
AATAGAC207.8570907E-444.00000411
GATACGC254.4433575E-544.041
CGCGAAC302.5281279E-644.037
AGTATTC254.4433575E-544.018
TTATATG254.4433575E-544.044
ATATCGT302.5281279E-644.035
ATAAACG302.5281279E-644.040
ATCGGAT254.4433575E-544.032
AATGGGA340150.041.9561964
ATGGGAG133000.041.9157875
GCGAATG1481100.041.8075751
Produced by FastQC (version 0.11.5)