##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527741_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1410969
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46806910711717	33.0	31.0	34.0	31.0	34.0
2	32.850955619861246	34.0	31.0	34.0	31.0	34.0
3	33.099591132051806	34.0	33.0	34.0	31.0	34.0
4	35.86653144044979	37.0	35.0	37.0	35.0	37.0
5	36.302642368471595	37.0	37.0	37.0	35.0	37.0
6	36.30418598849443	37.0	37.0	37.0	35.0	37.0
7	36.4947932945373	37.0	37.0	37.0	35.0	37.0
8	36.66428178081871	37.0	37.0	37.0	35.0	37.0
9	38.77414174230617	39.0	39.0	39.0	39.0	39.0
10	38.32636365504841	39.0	39.0	39.0	37.0	39.0
11	37.95543842564933	39.0	38.0	39.0	35.0	39.0
12	37.404719026427934	39.0	37.0	39.0	35.0	39.0
13	37.26584283566825	39.0	37.0	39.0	35.0	39.0
14	37.99799286873064	40.0	38.0	40.0	34.0	40.0
15	38.04891815482835	40.0	38.0	40.0	34.0	40.0
16	38.10740065869626	40.0	38.0	40.0	34.0	40.0
17	37.9638702196859	40.0	38.0	40.0	34.0	40.0
18	37.88038645781729	40.0	38.0	40.0	34.0	40.0
19	37.87676128958184	40.0	38.0	40.0	34.0	40.0
20	37.72820451760457	40.0	37.0	40.0	33.0	40.0
21	37.71778898048079	40.0	37.0	40.0	33.0	40.0
22	37.777618785387915	40.0	37.0	40.0	34.0	40.0
23	37.67302541728415	40.0	37.0	40.0	34.0	40.0
24	37.575376213084766	40.0	37.0	40.0	33.0	40.0
25	37.53781124886514	39.0	36.0	40.0	33.0	40.0
26	37.40406344859455	39.0	36.0	40.0	33.0	40.0
27	37.27718185162112	39.0	36.0	40.0	33.0	40.0
28	37.05091394637302	39.0	35.0	40.0	32.0	40.0
29	37.22534655261739	40.0	36.0	40.0	33.0	40.0
30	37.39707746945539	40.0	36.0	40.0	33.0	40.0
31	37.43389472057855	40.0	36.0	40.0	33.0	40.0
32	37.464492132711634	40.0	36.0	40.0	33.0	40.0
33	37.35011329093694	40.0	35.0	40.0	33.0	40.0
34	36.94057275531922	40.0	35.0	40.0	32.0	40.0
35	36.920981254726364	39.0	35.0	40.0	32.0	40.0
36	36.8820016598522	39.0	35.0	40.0	32.0	40.0
37	36.787021543350704	39.0	35.0	40.0	32.0	40.0
38	36.68609799364834	39.0	35.0	40.0	32.0	40.0
39	36.55611781690455	39.0	35.0	40.0	31.0	40.0
40	36.289364968330275	39.0	35.0	40.0	31.0	40.0
41	36.14373030165794	39.0	35.0	40.0	31.0	40.0
42	36.0712467814672	38.0	35.0	40.0	30.0	40.0
43	35.92775815769163	38.0	35.0	40.0	30.0	40.0
44	35.59537027390396	38.0	35.0	40.0	29.0	40.0
45	35.5061783781217	37.0	35.0	40.0	29.0	40.0
46	35.32819289438677	37.0	35.0	40.0	29.0	40.0
47	35.215951590715314	37.0	34.0	40.0	29.0	40.0
48	35.04112918143488	36.0	34.0	40.0	28.0	40.0
49	34.896189072899546	36.0	34.0	40.0	28.0	40.0
50	34.2923572381817	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	20.0
16	35.0
17	99.0
18	224.0
19	393.0
20	635.0
21	1088.0
22	1697.0
23	2630.0
24	3869.0
25	5323.0
26	7078.0
27	9395.0
28	12296.0
29	16556.0
30	22031.0
31	27767.0
32	35362.0
33	49634.0
34	100509.0
35	104092.0
36	133218.0
37	235209.0
38	461097.0
39	180707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.66448589586305	0.27406697099652794	0.04061038903051733	0.02083674410989894
8	99.70679724359643	0.21297420425253852	0.054430678491164584	0.02579787365987488
9	99.19296596877749	0.4241765765229427	0.10957008977518286	0.2732873649243889
10	65.7392189339383	23.173932240892604	4.40874321122576	6.678105613943325
11	37.176862142258265	27.31420747018538	18.003726517024823	17.505203870531528
12	31.37857741736353	17.838662649569198	25.930973678372805	24.85178625469447
13	24.145817519732894	17.097044655127082	29.908382111867805	28.84875571327223
14	25.483196299847833	16.731409407293853	29.765218087711354	28.020176205146957
15	25.719913052660974	16.708588211363963	28.39700943110727	29.17448930486779
16	31.784256067992988	17.001153108253973	24.96794755944319	26.24664326430985
17	32.17342124454896	17.422140387209073	22.49751766339303	27.906920704848936
18	30.009589154687312	19.15520468557424	23.249554029890096	27.585652129848352
19	31.528190909934946	19.44181622700428	21.485801601594364	27.54419126146641
20	30.224547810759837	20.047782764894198	22.517787421268647	27.20988200307732
21	32.335224941157456	20.021276158441466	22.966486152424327	24.677012747976747
22	32.478743331710334	17.90967767541314	23.48371934464896	26.127859648227563
23	31.403595684951263	19.198720879055458	22.80893485257295	26.58874858342033
24	31.140017959288972	19.1549211924571	22.552160961722052	27.152899886531877
25	29.508727689977597	19.68632904053881	22.41161924889916	28.393324020584437
26	28.529117223695206	18.319750469358294	23.483577598090392	29.667554708856116
27	28.497153374737504	16.90887609862442	25.002958959410165	29.59101156722791
28	31.93996466258295	20.085132983077585	20.970907227586146	27.003995126753317
29	32.123313836094205	20.641488225467747	20.94241616931343	26.292781769124623
30	30.826616318289062	19.359957589429673	23.23325317565446	26.5801729166268
31	32.26279244972781	19.783992419394046	20.855312909071706	27.09790222180643
32	34.020875015680716	20.724197342393776	20.356506769461273	24.898420872464243
33	35.38738271358194	19.193830622784766	20.869133198532356	24.549653465100935
34	30.6486535140035	21.98375726185338	24.544267095875245	22.82332212826788
35	30.018235694760126	22.82481046713287	24.092237320593153	23.06471651751385
36	30.87431403524812	23.461110768556928	21.241501407897694	24.423073788297263
37	31.057025349245805	24.186002669087696	19.657129249473236	25.099842732193267
38	32.361660674330906	22.855640343622007	20.757366037099327	24.025332944947763
39	32.86301825199561	21.734070698931017	19.620275144244843	25.782635904828528
40	29.76925786463062	21.864690152653957	21.023424327536606	27.34262765517882
41	28.55491509735508	21.805156598054246	22.28348035995121	27.356447944639463
42	27.830590183058597	19.370517708043195	22.590645152374005	30.208246956524203
43	26.477761028059437	18.74832118919693	25.803118282542	28.970799500201633
44	27.410453383454918	18.215283255691656	24.452982312155687	29.92128104869774
45	29.198373599986958	18.915086015355403	23.231339597113756	28.655200787543876
46	28.544851091696554	21.232642247987023	22.873004297046922	27.349502363269494
47	27.400318504517106	20.68996554849894	23.276415002739252	28.6333009442447
48	28.903469884880533	20.722921623366634	25.154840396918715	25.218768094834115
49	29.423467134997296	19.86677240959936	24.068707391870408	26.641053063532933
50	26.371947222086384	20.61675345099715	24.845265913000215	28.166033413916253
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	9.5
6	19.0
7	16.0
8	13.0
9	13.5
10	14.0
11	232.5
12	451.0
13	936.5
14	1422.0
15	2494.5
16	3567.0
17	3002.5
18	2438.0
19	2133.5
20	1829.0
21	2135.5
22	2442.0
23	2528.5
24	2615.0
25	3224.0
26	3833.0
27	4078.5
28	4324.0
29	4904.0
30	5484.0
31	6457.0
32	7430.0
33	8400.0
34	9370.0
35	12027.5
36	14685.0
37	14854.0
38	15023.0
39	16777.0
40	18531.0
41	21006.0
42	23481.0
43	25197.0
44	26913.0
45	27971.5
46	29030.0
47	33680.0
48	38330.0
49	41990.0
50	45650.0
51	49706.0
52	53762.0
53	59535.0
54	65308.0
55	72419.0
56	79530.0
57	105893.5
58	132257.0
59	134369.5
60	136482.0
61	145815.0
62	155148.0
63	154550.0
64	153952.0
65	136795.0
66	119638.0
67	100767.0
68	81896.0
69	69521.5
70	57147.0
71	48539.0
72	39931.0
73	34600.0
74	29269.0
75	29959.0
76	30649.0
77	21636.5
78	12624.0
79	8531.0
80	4438.0
81	3009.0
82	1580.0
83	949.5
84	319.0
85	195.0
86	71.0
87	65.0
88	59.0
89	36.0
90	13.0
91	6.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1410969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.378762833795138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.38459911868976	5.478420489982721
2	9.654732557512851	1.6178942864624113
3	3.8804568479313795	0.975402828467798
4	1.873590386369575	0.6279347798027708
5	1.1100073011503446	0.4650243960059877
6	0.71406519110619	0.3589789730488421
7	0.49484359662063904	0.2902323995134566
8	0.4040874592471569	0.27086023881142285
9	0.34158975367278804	0.2575889579230914
>10	9.786238143606555	23.869960593750942
>50	4.374742063754584	25.13301325099552
>100	1.8706088863608157	27.047390297424545
>500	0.06848911254410309	3.865767677838461
>1k	0.0376690118992567	6.276958851349596
>5k	0.002568341720403866	1.4565849934347916
>10k+	0.001712227813602577	2.0079869851876353
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	16504	1.1696926013257556	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	11489	0.8142631057096222	No Hit
GCGAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	7709	0.5463621100109215	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7190	0.5095788780618142	No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	5407	0.3832118210959986	No Hit
GCGAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4987	0.35344504379614294	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	4849	0.34366453125476176	No Hit
GCGAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4832	0.3424596855069105	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4373	0.3099288503149254	No Hit
GCGAATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	4118	0.2918561640971559	No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3676	0.26053017465302214	No Hit
GCGAATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	3427	0.24288272811096487	No Hit
GCGAATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	3327	0.23579540018242784	No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	3077	0.21807708036108517	No Hit
GCGAATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	2723	0.192987939494064	No Hit
GCGAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2575	0.18249869415982917	No Hit
GCGAATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	2498	0.17704145165485563	No Hit
GCGAATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	2278	0.1614493302120741	No Hit
GCGAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2151	0.15244842374283205	No Hit
GCGAATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	1948	0.13806114804790182	No Hit
GCGAATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1894	0.13423399096649183	No Hit
GCGAATGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1607	0.11389335981159046	No Hit
GCGAATGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	1563	0.11077493552303418	No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1510	0.10701865172090953	No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	1431	0.10141966265736525	No Hit
GCGAATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	1417	0.10042743674737006	No Hit
GCGAATGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA	1417	0.10042743674737006	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.05584814407687199	0.0	0.0	0.0	0.0
8	0.06555778333896776	0.0	0.0	0.0	0.0
9	0.08667802056600818	0.0	0.0	0.0	0.0
10	0.11460209260444418	0.0	0.0	0.0	0.0
11	0.14833777354428057	0.0	0.0	0.0	0.0
12	0.16251242940135469	0.0	0.0	0.0	0.0
13	0.17392302736629933	0.0	0.0	0.0	0.0
14	0.18256956743911454	0.0	0.0	0.0	0.0
15	0.19178309374621272	0.0	0.0	0.0	0.0
16	0.20489465041400626	0.0	0.0	0.0	0.0
17	0.21736834756823148	0.0	0.0	0.0	0.0
18	0.22962942488460059	0.0	0.0	0.0	0.0
19	0.24096914957025986	0.0	0.0	0.0	0.0
20	0.2528758604902021	0.0	0.0	0.0	0.0
21	0.26584567059942493	0.0	0.0	0.0	0.0
22	0.27626404265437443	0.0	0.0	0.0	0.0
23	0.28824162685360205	0.0	0.0	0.0	0.0
24	0.30021921105282967	0.0	0.0	0.0	0.0
25	0.31177155557634506	0.0	0.0	0.0	0.0
26	0.32424525273057025	0.0	0.0	0.0	0.0
27	0.33608109037122713	0.0	0.0	0.0	0.0
28	0.3496887599940183	0.0	0.0	0.0	0.0
29	0.3612411045175337	0.0	0.0	0.0	0.0
30	0.3723682093653369	0.0	0.0	0.0	0.0
31	0.3834244409338547	0.0	0.0	0.0	0.0
32	0.39802433646664104	0.0	0.0	0.0	0.0
33	0.40773397572873676	0.0	0.0	0.0	0.0
34	0.41864846073868384	0.0	0.0	0.0	0.0
35	0.43416970890218	0.0	0.0	0.0	0.0
36	0.4459346732635515	0.0	0.0	0.0	0.0
37	0.4581957505799206	0.0	0.0	0.0	0.0
38	0.47130730724771414	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAC	20	7.8570907E-4	44.000004	16
CTCGTAA	20	7.8570907E-4	44.000004	16
CGACGTT	20	7.8570907E-4	44.000004	37
TTATAGG	20	7.8570907E-4	44.000004	29
AAACGTT	40	8.314601E-9	44.000004	27
GCCGATT	20	7.8570907E-4	44.000004	19
TACTCGA	20	7.8570907E-4	44.000004	44
CGTGTAA	20	7.8570907E-4	44.000004	42
ATAACTA	20	7.8570907E-4	44.000004	37
AATAGAC	20	7.8570907E-4	44.000004	11
GATACGC	25	4.4433575E-5	44.0	41
CGCGAAC	30	2.5281279E-6	44.0	37
AGTATTC	25	4.4433575E-5	44.0	18
TTATATG	25	4.4433575E-5	44.0	44
ATATCGT	30	2.5281279E-6	44.0	35
ATAAACG	30	2.5281279E-6	44.0	40
ATCGGAT	25	4.4433575E-5	44.0	32
AATGGGA	34015	0.0	41.956196	4
ATGGGAG	13300	0.0	41.915787	5
GCGAATG	148110	0.0	41.807575	1
>>END_MODULE