FastQCFastQC Report
Fri 17 Jun 2016
SRR1527740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135623
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG252821.1838231747831895No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC177680.8319820492661859No Hit
GCGAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT116090.5435884517070663No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT88960.4165529215596573No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC85330.39955553953108763No Hit
GCGAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC78610.3680893116434876No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC74210.3474864243361305No Hit
GCGAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC73040.3420079293021287No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA66680.3122273921942215No Hit
GCGAATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT61420.2875975769131537No Hit
GCGAATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT51510.24119425572771974No Hit
GCGAATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG50610.2369800287784876No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT46490.21768823429978043No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC46300.21679856416605367No Hit
GCGAATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG43880.2054669761470072No Hit
GCGAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC40340.1888910168133608No Hit
GCGAATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG37870.17732530507491256No Hit
GCGAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG33280.15583274763382862No Hit
GCGAATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG32630.15278913928160542No Hit
GCGAATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT31130.14576542769955184No Hit
GCGAATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA28840.13504256135095005No Hit
GCGAATGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC23600.11050639555764291No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC23300.1091016532412322No Hit
GCGAATGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA21940.1027334880735036No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT21640.10132874575709289No Hit
GCGAATGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG21430.1003454261356054No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT21410.10025177664784468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC405.6152203E-946.00000414
CGTATGT206.3126755E-446.00000419
GCGTTAG206.3126755E-446.00000426
CGTAATA206.3126755E-446.00000422
CGGTAAT405.6152203E-946.00000445
TATCGTG206.3126755E-446.00000421
TATCGAG206.3126755E-446.00000423
CTCGTAA253.418149E-546.016
ATTCCGA253.418149E-546.019
ATATCGT750.046.035
CTCGATT301.8621595E-646.042
ATACGTT253.418149E-546.021
ATACGCT253.418149E-546.043
CGATCTA253.418149E-546.013
CGTTACA351.02012564E-745.99999634
GCGAATA6750.044.9777761
CGAATAG6450.044.573642
AATGGGA513650.043.8148544
CGAATGG2237150.043.6621172
GCGAATG2242450.043.658411
Produced by FastQC (version 0.11.5)