##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527740_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2135623
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14945193978525	33.0	31.0	34.0	31.0	34.0
2	32.56520977719382	34.0	31.0	34.0	31.0	34.0
3	32.85505306882348	34.0	31.0	34.0	31.0	34.0
4	35.71710550036219	37.0	35.0	37.0	35.0	37.0
5	36.07723273255626	37.0	35.0	37.0	35.0	37.0
6	36.042700420439374	37.0	35.0	37.0	35.0	37.0
7	36.391044674083396	37.0	36.0	37.0	35.0	37.0
8	36.58718182001224	37.0	37.0	37.0	35.0	37.0
9	38.70296770544239	39.0	39.0	39.0	38.0	39.0
10	38.131012823892604	39.0	38.0	39.0	37.0	39.0
11	37.70010343585923	39.0	38.0	39.0	35.0	39.0
12	37.142551845527045	39.0	37.0	39.0	34.0	39.0
13	36.99896704615	39.0	37.0	39.0	33.0	39.0
14	37.782725696436124	40.0	37.0	40.0	33.0	40.0
15	37.85934877082706	40.0	37.0	40.0	33.0	40.0
16	37.92520402711527	40.0	37.0	40.0	34.0	40.0
17	37.80944155405706	40.0	37.0	40.0	33.0	40.0
18	37.78952839522706	40.0	37.0	40.0	33.0	40.0
19	37.80039688652913	40.0	37.0	40.0	34.0	40.0
20	37.73942264154301	40.0	37.0	40.0	33.0	40.0
21	37.71979230416604	40.0	37.0	40.0	33.0	40.0
22	37.71475817595147	40.0	37.0	40.0	33.0	40.0
23	37.64845480686432	40.0	37.0	40.0	33.0	40.0
24	37.56023230691934	40.0	36.0	40.0	33.0	40.0
25	37.54913296962994	40.0	36.0	40.0	33.0	40.0
26	37.406084313570325	40.0	36.0	40.0	33.0	40.0
27	37.30360695684585	40.0	36.0	40.0	33.0	40.0
28	37.1531281504273	39.0	35.0	40.0	33.0	40.0
29	37.035482386170216	39.0	35.0	40.0	32.0	40.0
30	36.8951753188648	39.0	35.0	40.0	32.0	40.0
31	36.77716572634777	39.0	35.0	40.0	31.0	40.0
32	36.693492250270765	39.0	35.0	40.0	31.0	40.0
33	36.53042039723303	38.0	35.0	40.0	31.0	40.0
34	36.24299888135687	38.0	35.0	40.0	30.0	40.0
35	36.140923280934885	38.0	35.0	40.0	30.0	40.0
36	36.133186428503535	38.0	35.0	40.0	30.0	40.0
37	36.018270546814676	38.0	35.0	40.0	30.0	40.0
38	35.92346355138524	38.0	35.0	40.0	30.0	40.0
39	35.81144612134258	38.0	34.0	40.0	30.0	40.0
40	35.5072402760225	38.0	34.0	40.0	29.0	40.0
41	35.34965253698804	38.0	34.0	40.0	28.0	40.0
42	35.27798632998427	37.0	34.0	40.0	28.0	40.0
43	35.10779617938185	37.0	34.0	40.0	28.0	40.0
44	34.92647204117955	37.0	34.0	40.0	28.0	40.0
45	34.771809912142736	36.0	33.0	40.0	27.0	40.0
46	34.56565133452861	36.0	33.0	39.0	27.0	40.0
47	34.3548627262396	36.0	33.0	39.0	26.0	40.0
48	34.0663932725954	35.0	33.0	39.0	26.0	40.0
49	34.45824145928378	35.0	33.0	39.0	27.0	40.0
50	34.569411829709644	35.0	33.0	39.0	27.0	40.0
51	34.58091760577592	35.0	34.0	39.0	28.0	40.0
52	34.078500278373106	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	16.0
16	45.0
17	133.0
18	323.0
19	610.0
20	1128.0
21	1899.0
22	3365.0
23	4887.0
24	7473.0
25	10307.0
26	14335.0
27	18835.0
28	24552.0
29	32122.0
30	41217.0
31	52405.0
32	68300.0
33	95345.0
34	171857.0
35	175088.0
36	244926.0
37	374714.0
38	616717.0
39	175021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.71839599030353	0.2495290601384233	0.01713785626021072	0.014937093297833935
8	99.72935298037153	0.2105240484860858	0.03886453742069644	0.02125843372168215
9	99.19981195182858	0.41247916884206626	0.1102722718382411	0.2774366074911162
10	65.7058385304897	23.252231316107757	4.378300851788916	6.663629301613627
11	37.22042701356934	27.455922697966823	17.8320799129809	17.49157037548294
12	31.34630971852242	17.85610100659152	25.90906728387922	24.88852199100684
13	24.10359880934041	17.077967412787746	29.943065793915874	28.875367983955968
14	25.498882527487293	16.683937193034538	29.71947764188717	28.097702637591
15	25.727527751855078	16.729076246135204	28.261495591684486	29.28190041032523
16	31.858759715549045	16.98310048168614	24.924249270587552	26.233890532177263
17	32.211162737992616	17.432383899218166	22.496901372573717	27.859551990215504
18	30.025383693657542	19.104870101136765	23.221139686171202	27.64860651903449
19	31.53922766330949	19.306544273029463	21.476168780725814	27.678059282935237
20	30.200742359489478	19.993791038961465	22.523404177609997	27.282062423939056
21	32.48639858252135	19.954270955126443	22.92726759357808	24.632062868774124
22	32.4741304996247	17.9088256682008	23.48317095292568	26.13387287924882
23	31.454943124324846	19.198706887872998	22.729058452732527	26.617291535069626
24	31.18237629019729	19.05340970761225	22.584604117861627	27.179609884328837
25	29.476831819099157	19.657167955205576	22.30192313905591	28.564077086639355
26	28.475812444424882	18.240813102312533	23.52367435638219	29.759700096880394
27	28.47955842393531	16.886875633012004	24.9908808811293	29.642685061923384
28	31.887556933035466	20.135248590224023	20.891234080172392	27.08596039656812
29	32.105619765286285	20.581020151965024	20.876624760081718	26.43673532266697
30	30.907374569387947	19.343629470182705	23.158581828347042	26.5904141320823
31	32.40187991981731	19.813188001814925	20.767335807864963	27.017596270502796
32	33.979452365890424	20.742518693608375	20.385760969983934	24.892267970517267
33	35.48823926320329	19.11381362721791	20.77876104537177	24.619186064207025
34	30.501965936871816	21.97700624127011	24.553537773286767	22.967490048571307
35	29.976030413607646	22.89458392234959	24.130195263864458	22.99919040017831
36	30.905876177583778	23.463129962544887	21.184076028400145	24.44691783147119
37	31.15259575308938	24.095498128649112	19.602757602816602	25.149148515444907
38	32.337636371213456	22.829216579892613	20.819779521010965	24.013367527882963
39	32.849337172338	21.63457688927306	19.660913934716007	25.855172003672934
40	29.687074919121958	21.767371862917752	21.067435591394172	27.47811762656611
41	28.549561416036447	21.875677495513017	22.225083734348246	27.34967735410229
42	27.840541144200078	19.271285240887554	22.5920492521386	30.296124362773767
43	26.47850299420825	18.65034231229014	25.713714452410375	29.157440241091244
44	27.280657681622642	18.101603138756232	24.417277768594925	30.200461411026197
45	29.40636057955922	18.802616379389057	23.200349499888322	28.590673541163397
46	28.381788358713123	21.14694400650302	22.942064212644272	27.52920342213958
47	27.417339109009408	20.65917064950134	23.260238347311297	28.66325189417795
48	28.97098411095966	20.698924857055765	25.105929276843337	25.22416175514124
49	29.48502614927822	19.70488236921966	24.10327103613325	26.706820445368866
50	26.363407773750332	20.501230788392895	24.983576221083965	28.15178521677281
51	27.11447666559126	20.273475234158838	24.083136396264695	28.528911703985205
52	27.532153381004044	20.669565742642778	23.519319655201315	28.27896122115186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	12.0
6	24.0
7	20.5
8	17.0
9	19.0
10	21.0
11	333.0
12	645.0
13	1340.0
14	3519.5
15	5004.0
16	4180.0
17	3356.0
18	2843.5
19	2331.0
20	2651.0
21	2971.0
22	3151.5
23	3332.0
24	4424.5
25	5517.0
26	5379.0
27	5241.0
28	5786.5
29	6332.0
30	7429.5
31	8527.0
32	10261.0
33	11995.0
34	13019.5
35	14044.0
36	18416.0
37	22788.0
38	24287.5
39	26644.0
40	27501.0
41	31970.0
42	36439.0
43	36442.5
44	36446.0
45	41159.5
46	45873.0
47	50619.5
48	55366.0
49	60492.5
50	65619.0
51	69929.0
52	74239.0
53	86402.0
54	98565.0
55	106626.5
56	114688.0
57	140862.0
58	167036.0
59	196586.5
60	226137.0
61	220929.0
62	215721.0
63	225119.5
64	206104.0
65	177690.0
66	160035.5
67	142381.0
68	116456.5
69	90532.0
70	79626.0
71	68720.0
72	58804.5
73	48889.0
74	50079.0
75	51269.0
76	38016.5
77	24764.0
78	16387.0
79	8010.0
80	5951.0
81	3892.0
82	2481.5
83	1071.0
84	629.5
85	188.0
86	126.5
87	65.0
88	42.0
89	16.5
90	14.0
91	9.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2135623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.678266605892345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.80071284756576	5.205919493131604
2	9.939586475353899	1.5263758982017805
3	4.306437985236416	0.9919793691716127
4	2.251915949015302	0.6916324412240225
5	1.2628326940304093	0.4848183051701379
6	0.8030493715646952	0.369961630354082
7	0.5245477633980791	0.28193323024264927
8	0.3630342811704057	0.22299791983238867
9	0.28867351170889416	0.19948609664640674
>10	5.116449974376225	11.144967352196119
>50	4.4241938052020116	24.572280545479167
>100	2.7594041307428117	37.159586824561316
>500	0.10732709989187512	5.500165930821472
>1k	0.04451635393242547	6.137006548400051
>5k	0.00548831760810725	2.9515860606058992
>10k+	0.0018294392027024167	2.5593023539612902
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	25282	1.1838231747831895	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	17768	0.8319820492661859	No Hit
GCGAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	11609	0.5435884517070663	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8896	0.4165529215596573	No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	8533	0.39955553953108763	No Hit
GCGAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	7861	0.3680893116434876	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	7421	0.3474864243361305	No Hit
GCGAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	7304	0.3420079293021287	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6668	0.3122273921942215	No Hit
GCGAATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6142	0.2875975769131537	No Hit
GCGAATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	5151	0.24119425572771974	No Hit
GCGAATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	5061	0.2369800287784876	No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4649	0.21768823429978043	No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4630	0.21679856416605367	No Hit
GCGAATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	4388	0.2054669761470072	No Hit
GCGAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	4034	0.1888910168133608	No Hit
GCGAATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3787	0.17732530507491256	No Hit
GCGAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3328	0.15583274763382862	No Hit
GCGAATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	3263	0.15278913928160542	No Hit
GCGAATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	3113	0.14576542769955184	No Hit
GCGAATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2884	0.13504256135095005	No Hit
GCGAATGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2360	0.11050639555764291	No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	2330	0.1091016532412322	No Hit
GCGAATGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA	2194	0.1027334880735036	No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2164	0.10132874575709289	No Hit
GCGAATGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	2143	0.1003454261356054	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	2141	0.10025177664784468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0467779191364768	0.0	0.0	0.0	0.0
8	0.05558097098598395	0.0	0.0	0.0	0.0
9	0.07182915711246789	0.0	0.0	0.0	0.0
10	0.09692721983233932	0.0	0.0	0.0	0.0
11	0.12520936513607506	0.0	0.0	0.0	0.0
12	0.13771157175213042	0.0	0.0	0.0	0.0
13	0.14628049988223576	0.0	0.0	0.0	0.0
14	0.1533510362081697	0.0	0.0	0.0	0.0
15	0.16177949010663398	0.0	0.0	0.0	0.0
16	0.173579325564484	0.0	0.0	0.0	0.0
17	0.1839744187059233	0.0	0.0	0.0	0.0
18	0.19287112004319115	0.0	0.0	0.0	0.0
19	0.20378128536731435	0.0	0.0	0.0	0.0
20	0.21450415171591616	0.0	0.0	0.0	0.0
21	0.2246182963940733	0.0	0.0	0.0	0.0
22	0.23257850285373402	0.0	0.0	0.0	0.0
23	0.24208392586144653	0.0	0.0	0.0	0.0
24	0.2532750396488519	0.0	0.0	0.0	0.0
25	0.2630145863759662	0.0	0.0	0.0	0.0
26	0.2744866486266537	0.0	0.0	0.0	0.0
27	0.2858182366457001	0.0	0.0	0.0	0.0
28	0.29897598967608047	0.0	0.0	0.0	0.0
29	0.30918378384199835	0.0	0.0	0.0	0.0
30	0.3203748976294037	0.0	0.0	0.0	0.0
31	0.3297398464054751	0.0	0.0	0.0	0.0
32	0.3453793108615144	0.0	0.0	0.0	0.0
33	0.3551188575886287	0.0	0.0	0.0	0.0
34	0.36513935277902515	0.0	0.0	0.0	0.0
35	0.379280425430893	0.0	0.0	0.0	0.0
36	0.3903310649866573	0.0	0.0	0.0	0.0
37	0.4022713746761484	0.0	0.0	0.0	0.0
38	0.41355613795131446	0.0	0.0	0.0	0.0
39	0.42437265378767697	0.0	0.0	0.0	0.0
40	0.43593836552612514	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAC	40	5.6152203E-9	46.000004	14
CGTATGT	20	6.3126755E-4	46.000004	19
GCGTTAG	20	6.3126755E-4	46.000004	26
CGTAATA	20	6.3126755E-4	46.000004	22
CGGTAAT	40	5.6152203E-9	46.000004	45
TATCGTG	20	6.3126755E-4	46.000004	21
TATCGAG	20	6.3126755E-4	46.000004	23
CTCGTAA	25	3.418149E-5	46.0	16
ATTCCGA	25	3.418149E-5	46.0	19
ATATCGT	75	0.0	46.0	35
CTCGATT	30	1.8621595E-6	46.0	42
ATACGTT	25	3.418149E-5	46.0	21
ATACGCT	25	3.418149E-5	46.0	43
CGATCTA	25	3.418149E-5	46.0	13
CGTTACA	35	1.02012564E-7	45.999996	34
GCGAATA	675	0.0	44.977776	1
CGAATAG	645	0.0	44.57364	2
AATGGGA	51365	0.0	43.814854	4
CGAATGG	223715	0.0	43.662117	2
GCGAATG	224245	0.0	43.65841	1
>>END_MODULE