FastQCFastQC Report
Fri 17 Jun 2016
SRR1527739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151580
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG239111.1113228418185706No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC167620.7790553918515696No Hit
GCGAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT113680.5283559058924139No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT85260.3962669294193105No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC80570.3744689948781825No Hit
GCGAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC74890.34806979057250953No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC70350.32696901811691875No Hit
GCGAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC69820.3245057120813542No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA63570.29545729185063996No Hit
GCGAATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT59410.2761226633450767No Hit
GCGAATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT49350.2293663261417191No Hit
GCGAATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG48230.22416084923637514No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC44950.20891623829929631No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT41830.1944152669201238No Hit
GCGAATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG40320.18739716859238328No Hit
GCGAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC37460.17410461149480846No Hit
GCGAATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG34410.15992898242221995No Hit
GCGAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG31130.14468437148514116No Hit
GCGAATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG30630.14236049786668403No Hit
GCGAATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT30350.14105912864034803No Hit
GCGAATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA26740.12428076111508753No Hit
GCGAATGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC23140.10754887106219616No Hit
GCGAATGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA21730.10099554745814704No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT21650.10062372767919389No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC21610.10043781778971733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTT351.02012564E-746.00000414
ACGTAAG351.02012564E-746.00000438
CTCGTAA253.4181572E-546.016
CTAACGT206.312688E-446.039
ACAATCG206.312688E-446.042
ATACGTA206.312688E-446.034
ATACCGA253.4181572E-546.027
ATTTCGT206.312688E-446.012
TAATCGA301.8621649E-646.031
AATGGGA521650.043.6724
CGAATGG2253250.043.666572
GCGAATG2260800.043.6397741
GAATGGG2238950.043.6023563
AATGGGG1475400.043.4309354
ATGGGAT141800.043.3723535
ATGGGGA440800.043.3493655
AATGGGT97200.043.231484
ATGGGGG565950.043.167425
ATGGGAG201600.043.159235
ATGGGAC88650.043.1201325
Produced by FastQC (version 0.11.5)