##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527739_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2151580
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094310692607294	33.0	31.0	34.0	31.0	34.0
2	32.53520854441852	34.0	31.0	34.0	31.0	34.0
3	32.83885842032367	34.0	31.0	34.0	31.0	34.0
4	35.681847758391505	37.0	35.0	37.0	35.0	37.0
5	36.082723393970944	37.0	35.0	37.0	35.0	37.0
6	35.984529508547205	37.0	35.0	37.0	35.0	37.0
7	36.34991634054973	37.0	36.0	37.0	35.0	37.0
8	36.56125637903308	37.0	37.0	37.0	35.0	37.0
9	38.657908141923606	39.0	39.0	39.0	38.0	39.0
10	38.03749058831185	39.0	38.0	39.0	37.0	39.0
11	37.65897015216724	39.0	38.0	39.0	35.0	39.0
12	37.098028890396826	39.0	37.0	39.0	34.0	39.0
13	36.801880013757334	39.0	37.0	39.0	33.0	39.0
14	37.717761830840594	40.0	37.0	40.0	33.0	40.0
15	37.818842432073176	40.0	37.0	40.0	33.0	40.0
16	37.91196701958561	40.0	37.0	40.0	34.0	40.0
17	37.82632576989933	40.0	37.0	40.0	33.0	40.0
18	37.81956422721907	40.0	37.0	40.0	33.0	40.0
19	37.784267840377765	40.0	37.0	40.0	33.0	40.0
20	37.7349942832709	40.0	37.0	40.0	33.0	40.0
21	37.777096831165935	40.0	37.0	40.0	33.0	40.0
22	37.654770447763966	40.0	37.0	40.0	33.0	40.0
23	37.62048587549614	40.0	37.0	40.0	33.0	40.0
24	37.57969631619554	40.0	36.0	40.0	33.0	40.0
25	37.53152799338161	40.0	36.0	40.0	33.0	40.0
26	37.3410837617007	40.0	36.0	40.0	33.0	40.0
27	37.24036800862622	40.0	36.0	40.0	33.0	40.0
28	37.10951579769286	39.0	35.0	40.0	32.0	40.0
29	36.996723802972696	39.0	35.0	40.0	32.0	40.0
30	36.84384731220777	39.0	35.0	40.0	31.0	40.0
31	36.74361446007121	39.0	35.0	40.0	31.0	40.0
32	36.66607562814304	39.0	35.0	40.0	31.0	40.0
33	36.363044367395126	39.0	35.0	40.0	31.0	40.0
34	36.17356314894171	38.0	35.0	40.0	30.0	40.0
35	36.127926454047724	38.0	35.0	40.0	30.0	40.0
36	35.97471671980591	38.0	35.0	40.0	30.0	40.0
37	35.9395495403378	38.0	35.0	40.0	30.0	40.0
38	35.557760343561476	38.0	34.0	40.0	29.0	40.0
39	35.62309140259716	38.0	34.0	40.0	29.0	40.0
40	35.389220015058704	38.0	34.0	40.0	28.0	40.0
41	35.041888286747415	37.0	34.0	40.0	27.0	40.0
42	35.11625410163693	37.0	34.0	40.0	28.0	40.0
43	35.05091002890899	37.0	34.0	40.0	28.0	40.0
44	34.8705811543145	37.0	33.0	40.0	27.0	40.0
45	34.725095975980445	36.0	33.0	40.0	27.0	40.0
46	34.346183269969046	36.0	33.0	39.0	26.0	40.0
47	34.05955576831909	35.0	33.0	39.0	25.0	40.0
48	33.93751754524582	35.0	33.0	39.0	25.0	40.0
49	33.81217756253544	35.0	32.0	39.0	26.0	40.0
50	33.64110746521161	35.0	32.0	39.0	25.0	40.0
51	34.07517638200764	35.0	33.0	39.0	26.0	40.0
52	33.82031530317255	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	16.0
16	49.0
17	146.0
18	345.0
19	653.0
20	1196.0
21	2214.0
22	3513.0
23	5302.0
24	7891.0
25	11048.0
26	15107.0
27	19856.0
28	26095.0
29	33617.0
30	43127.0
31	54924.0
32	73007.0
33	102361.0
34	175143.0
35	182089.0
36	259775.0
37	390493.0
38	594236.0
39	149371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.71132841911525	0.2499093689288802	0.02235566420955763	0.016406547746307367
8	99.73321930860112	0.20459383336896608	0.0412719954637987	0.020914862566114203
9	99.16168583087777	0.4233632958105206	0.12953271549280063	0.28541815781890517
10	65.63204714674798	23.28753753055894	4.386404409782578	6.694010912910513
11	37.094367859898306	27.530744847972187	17.86733470286952	17.507552589259987
12	31.40269011610072	17.812258898112084	25.942051887450152	24.842999098337035
13	24.295401518883796	17.16315451900464	29.820689911599846	28.720754050511715
14	25.51473800648826	16.70102901123825	29.75181029754878	28.03242268472471
15	25.726768235436282	16.766329859916898	28.262067875700648	29.24483402894617
16	31.873321001310668	16.9940694745257	24.927588098048876	26.20502142611476
17	32.218183846289705	17.38048317980275	22.51647626395486	27.884856709952686
18	29.972067039106143	19.14444268862882	23.23762072523448	27.645869547030554
19	31.55755305403471	19.32277675010922	21.431366716552485	27.688303479303585
20	30.18683943892395	19.956450608390114	22.463120125675083	27.39358982701085
21	32.4278437241469	19.954173212244026	22.993335130462263	24.624647933146804
22	32.6837486870114	17.85906171278781	23.405357923014712	26.051831677186065
23	31.50275611411149	19.23070487734595	22.66557599531507	26.600963013227492
24	31.190055680011895	19.064269048792053	22.56867046542541	27.177004805770643
25	29.499298190167227	19.670056423651456	22.33061285195066	28.500032534230655
26	28.586573587782	18.321837905167364	23.44579332397587	29.645795183074764
27	28.52057557701782	16.875412487567274	24.982385037972097	29.62162689744281
28	31.890796530921463	20.137480363267922	20.91899906115506	27.05272404465556
29	32.17802731016277	20.57450803595497	20.87940025469655	26.368064399185716
30	30.87991150689261	19.384219968581228	23.16255960735831	26.57330891716785
31	32.32471021295978	19.862054862008385	20.745916954052372	27.067317970979467
32	33.986419282573735	20.750750611178763	20.381394138261186	24.881435967986317
33	35.406910270591844	19.174141793472703	20.877494678329413	24.541453257606037
34	30.532631833350376	21.99881017670735	24.509523234088437	22.95903475585384
35	30.004787179654024	22.86454605452737	24.074494092713262	23.056172673105348
36	31.058431478262488	23.424227776796585	21.197306165701484	24.320034579239444
37	31.190195112429002	24.06529155318417	19.690413556549142	25.054099777837678
38	32.349808048039115	22.90419133845825	20.678524619117113	24.06747599438552
39	32.86779947759321	21.64581377406371	19.643285399566828	25.843101348776248
40	29.72401676907203	21.77111703957092	21.058477955734855	27.446388235622194
41	28.770066648695376	21.863421299696036	22.236449492930777	27.13006255867781
42	27.799709980572416	19.321150038576302	22.6085946141905	30.270545366660777
43	26.410358899041636	18.67185974957938	25.795740804432093	29.12204054694689
44	27.424636778553435	18.098095353182313	24.39886037237751	30.078407495886744
45	29.387845211426022	18.80088121287612	23.1622807425241	28.648992833173757
46	28.55915187908421	21.165329664711514	22.879047025906544	27.396471430297737
47	27.58591360767436	20.658818170832596	23.24645144498462	28.50881677650843
48	28.9519794755482	20.641993325834967	25.11061638423856	25.295410814378272
49	29.537316762565187	19.74307253274338	24.02573922419803	26.693871480493403
50	26.45056191264094	20.511902880673738	24.914620883257886	28.122914323427434
51	27.125833108692216	20.280119725968824	24.065942237797337	28.52810492754162
52	27.619005568001192	20.695210031697638	23.459132358545812	28.22665204175536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	13.0
6	26.0
7	27.5
8	29.0
9	21.5
10	14.0
11	303.5
12	593.0
13	1273.0
14	3488.0
15	5023.0
16	4273.0
17	3523.0
18	2963.0
19	2403.0
20	2738.0
21	3073.0
22	3243.5
23	3414.0
24	4445.0
25	5476.0
26	5439.0
27	5402.0
28	5839.5
29	6277.0
30	7476.0
31	8675.0
32	10368.5
33	12062.0
34	13134.0
35	14206.0
36	18539.0
37	22872.0
38	24318.5
39	26739.5
40	27714.0
41	32186.5
42	36659.0
43	36728.5
44	36798.0
45	41455.0
46	46112.0
47	50900.0
48	55688.0
49	60856.5
50	66025.0
51	70975.5
52	75926.0
53	87800.5
54	99675.0
55	108057.5
56	116440.0
57	141834.5
58	167229.0
59	197116.5
60	227004.0
61	222091.5
62	217179.0
63	225913.0
64	206947.5
65	179248.0
66	161307.5
67	143367.0
68	117813.5
69	92260.0
70	80980.0
71	69700.0
72	59504.0
73	49308.0
74	50256.0
75	51204.0
76	38163.0
77	25122.0
78	16689.5
79	8257.0
80	6028.0
81	3799.0
82	2475.5
83	1152.0
84	673.0
85	194.0
86	125.0
87	56.0
88	36.0
89	14.5
90	13.0
91	7.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2151580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.17352754722701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.01690588435677	5.5593805392293705
2	10.090311741331705	1.6494688195576566
3	4.354930786440911	1.0678544024772532
4	2.176568259428896	0.7116096250744812
5	1.2903462555638656	0.527334033265624
6	0.7436290341850527	0.3646843437497606
7	0.5053929350511254	0.2891590153769999
8	0.3782372516372775	0.24732260765157782
9	0.29634453224353047	0.21799621779863398
>10	5.238195555264428	12.19880113301486
>50	4.284284427019815	25.20015593382877
>100	2.487361019799359	35.72264414784471
>500	0.08928072576451396	4.923788713183297
>1k	0.04225954352853661	6.336756990880121
>5k	0.004166433869010652	2.4512888241395787
>10k+	0.0017856145152902792	2.531754652927299
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCGAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	23911	1.1113228418185706	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	16762	0.7790553918515696	No Hit
GCGAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	11368	0.5283559058924139	No Hit
GCGAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8526	0.3962669294193105	No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	8057	0.3744689948781825	No Hit
GCGAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	7489	0.34806979057250953	No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	7035	0.32696901811691875	No Hit
GCGAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6982	0.3245057120813542	No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6357	0.29545729185063996	No Hit
GCGAATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5941	0.2761226633450767	No Hit
GCGAATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	4935	0.2293663261417191	No Hit
GCGAATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	4823	0.22416084923637514	No Hit
GCGAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4495	0.20891623829929631	No Hit
GCGAATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4183	0.1944152669201238	No Hit
GCGAATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	4032	0.18739716859238328	No Hit
GCGAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3746	0.17410461149480846	No Hit
GCGAATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3441	0.15992898242221995	No Hit
GCGAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3113	0.14468437148514116	No Hit
GCGAATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	3063	0.14236049786668403	No Hit
GCGAATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	3035	0.14105912864034803	No Hit
GCGAATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2674	0.12428076111508753	No Hit
GCGAATGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2314	0.10754887106219616	No Hit
GCGAATGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA	2173	0.10099554745814704	No Hit
GCGAATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2165	0.10062372767919389	No Hit
GCGAATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	2161	0.10043781778971733	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.04880134598759981	0.0	0.0	0.0	0.0
8	0.058375705295643204	0.0	0.0	0.0	0.0
9	0.07478225304195056	0.0	0.0	0.0	0.0
10	0.09783507933704533	0.0	0.0	0.0	0.0
11	0.12572156275853094	0.0	0.0	0.0	0.0
12	0.13715502096134005	0.0	0.0	0.0	0.0
13	0.14631108301806114	0.0	0.0	0.0	0.0
14	0.15346861376290913	0.0	0.0	0.0	0.0
15	0.16109091923144853	0.0	0.0	0.0	0.0
16	0.17103709831844505	0.0	0.0	0.0	0.0
17	0.18168043949097873	0.0	0.0	0.0	0.0
18	0.19162661857797525	0.0	0.0	0.0	0.0
19	0.2036642839215832	0.0	0.0	0.0	0.0
20	0.21351750806384145	0.0	0.0	0.0	0.0
21	0.22355664209557627	0.0	0.0	0.0	0.0
22	0.23224792942860595	0.0	0.0	0.0	0.0
23	0.2426588832392939	0.0	0.0	0.0	0.0
24	0.2521867650749682	0.0	0.0	0.0	0.0
25	0.26217942163433383	0.0	0.0	0.0	0.0
26	0.2734734474200355	0.0	0.0	0.0	0.0
27	0.28276894189386403	0.0	0.0	0.0	0.0
28	0.2961544539361771	0.0	0.0	0.0	0.0
29	0.30400914676656227	0.0	0.0	0.0	0.0
30	0.31381589343645133	0.0	0.0	0.0	0.0
31	0.32436627966424675	0.0	0.0	0.0	0.0
32	0.33798417906840555	0.0	0.0	0.0	0.0
33	0.3474191059593415	0.0	0.0	0.0	0.0
34	0.3579694921871369	0.0	0.0	0.0	0.0
35	0.37195921137024884	0.0	0.0	0.0	0.0
36	0.3844151739651791	0.0	0.0	0.0	0.0
37	0.3955697673337733	0.0	0.0	0.0	0.0
38	0.406863793119475	0.0	0.0	0.0	0.0
39	0.41680997220647154	0.0	0.0	0.0	0.0
40	0.42694206118294464	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTT	35	1.02012564E-7	46.000004	14
ACGTAAG	35	1.02012564E-7	46.000004	38
CTCGTAA	25	3.4181572E-5	46.0	16
CTAACGT	20	6.312688E-4	46.0	39
ACAATCG	20	6.312688E-4	46.0	42
ATACGTA	20	6.312688E-4	46.0	34
ATACCGA	25	3.4181572E-5	46.0	27
ATTTCGT	20	6.312688E-4	46.0	12
TAATCGA	30	1.8621649E-6	46.0	31
AATGGGA	52165	0.0	43.672	4
CGAATGG	225325	0.0	43.66657	2
GCGAATG	226080	0.0	43.639774	1
GAATGGG	223895	0.0	43.602356	3
AATGGGG	147540	0.0	43.430935	4
ATGGGAT	14180	0.0	43.372353	5
ATGGGGA	44080	0.0	43.349365	5
AATGGGT	9720	0.0	43.23148	4
ATGGGGG	56595	0.0	43.16742	5
ATGGGAG	20160	0.0	43.15923	5
ATGGGAC	8865	0.0	43.120132	5
>>END_MODULE