FastQCFastQC Report
Fri 17 Jun 2016
SRR1527738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598345
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC73861.2344049001830049No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42150.7044430888534207No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGA36910.6168681947705754No Hit
GTCCCGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG19170.32038372510842406No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGC18640.3115259591038615No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA17700.29581595902029767No Hit
GTCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC15800.2640617035322431No Hit
GTCCCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC14900.24902021409053304No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG14240.23798978849994568No Hit
GTCCCGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA11660.19487085210037688No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC11030.18434180949117984No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG10930.1826705328865454Illumina Single End Adapter 2 (100% over 23bp)
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC10640.17782383073310548No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG9960.16645914982159124No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG9960.16645914982159124No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT9510.1589384051007362No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC8690.1452339369427337No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA8180.13671042625909802No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT7890.1318637241056581No Hit
GTCCCGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA7860.13136234112426778Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT7840.13102808580334088No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC7780.1300253198405602No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC7520.12568000066851065No Hit
GTCCCGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG6710.11214266017097159No Hit
GTCCCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG6690.1118084048500447No Hit
GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT6480.10829872398031236No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGACACCTACTGCT6400.10696170269660481No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG6290.10512329843150692No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGG6210.10378627714779935No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC6200.1036191494873359No Hit
GTCCCGGGGACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTGTC6030.10077797925945733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA207.853451E-444.00000430
GAAATCG207.853451E-444.00000420
AGGTAAT207.853451E-444.00000416
CGAACTA207.853451E-444.00000430
AAATCGT207.853451E-444.00000421
CGAACGA207.853451E-444.00000429
CATATCG207.853451E-444.00000436
CGACGAA207.853451E-444.00000427
GCTATAA207.853451E-444.00000417
TAAACTA207.853451E-444.00000435
ATTCGAG207.853451E-444.00000423
CGCGAAT207.853451E-444.00000419
ATTCCTA207.853451E-444.00000441
ATATCTC207.853451E-444.00000432
GTTAATC207.853451E-444.00000436
AGTTGCG207.853451E-444.00000421
TGATTAG207.853451E-444.00000413
TCCGTTA207.853451E-444.00000444
GAACGAC207.853451E-444.0000049
AATTCGT207.853451E-444.00000413
Produced by FastQC (version 0.11.5)