##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527738_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 598345 Sequences flagged as poor quality 0 Sequence length 50 %GC 61 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29475469837635 33.0 31.0 34.0 31.0 34.0 2 32.15855066892846 33.0 31.0 34.0 30.0 34.0 3 32.758171289139206 34.0 31.0 34.0 31.0 34.0 4 36.44000200553192 37.0 37.0 37.0 35.0 37.0 5 36.50710041865479 37.0 37.0 37.0 35.0 37.0 6 36.161766205115775 37.0 37.0 37.0 35.0 37.0 7 36.51815758467105 37.0 37.0 37.0 35.0 37.0 8 36.59680117657873 37.0 37.0 37.0 35.0 37.0 9 38.58143545947572 39.0 39.0 39.0 37.0 39.0 10 37.905691532477086 39.0 38.0 39.0 35.0 39.0 11 36.70744303035874 38.0 35.0 39.0 33.0 39.0 12 36.44411835980914 39.0 35.0 39.0 33.0 39.0 13 36.33621238583092 38.0 35.0 39.0 33.0 39.0 14 36.839435442762955 39.0 35.0 40.0 32.0 40.0 15 37.07763246956188 39.0 35.0 40.0 33.0 40.0 16 36.98511393928252 39.0 35.0 40.0 33.0 40.0 17 37.034344734225236 39.0 35.0 40.0 33.0 40.0 18 37.00987055962697 39.0 35.0 40.0 33.0 40.0 19 36.47521078976176 38.0 35.0 40.0 31.0 40.0 20 36.491014381335184 38.0 35.0 40.0 31.0 40.0 21 36.529968496436005 38.0 35.0 40.0 31.0 40.0 22 36.62497388630305 38.0 35.0 40.0 32.0 40.0 23 36.55472845933366 38.0 35.0 40.0 32.0 40.0 24 36.52097368574986 38.0 35.0 40.0 32.0 40.0 25 36.36946911898654 38.0 35.0 40.0 31.0 40.0 26 36.17562944455122 38.0 35.0 40.0 31.0 40.0 27 36.005401565986176 38.0 35.0 40.0 31.0 40.0 28 35.76399903065957 37.0 34.0 40.0 30.0 40.0 29 35.92067954106744 38.0 35.0 40.0 31.0 40.0 30 35.943243446506614 37.0 35.0 40.0 31.0 40.0 31 35.855330954549636 37.0 35.0 40.0 31.0 40.0 32 35.56160074873192 37.0 35.0 40.0 30.0 40.0 33 35.61672780753579 36.0 35.0 40.0 30.0 40.0 34 35.10994493143588 36.0 34.0 40.0 29.0 40.0 35 35.15786210296735 36.0 34.0 40.0 29.0 40.0 36 34.98665318503539 35.0 34.0 40.0 29.0 40.0 37 34.48201789937244 35.0 34.0 39.0 26.0 40.0 38 34.43320826613409 35.0 34.0 39.0 27.0 40.0 39 34.199359901060426 35.0 34.0 39.0 26.0 40.0 40 33.8361330001922 35.0 33.0 39.0 23.0 40.0 41 33.55113020080388 35.0 33.0 39.0 23.0 40.0 42 33.45830916945909 35.0 33.0 39.0 23.0 40.0 43 33.40454754364121 35.0 33.0 38.0 23.0 40.0 44 33.09842147924692 35.0 33.0 37.0 23.0 40.0 45 33.034162565075334 35.0 33.0 37.0 23.0 40.0 46 32.940962153941285 35.0 33.0 37.0 23.0 40.0 47 32.82531984056021 35.0 33.0 37.0 23.0 40.0 48 32.700171305851974 35.0 33.0 36.0 22.0 40.0 49 32.69115643984658 35.0 33.0 36.0 23.0 40.0 50 31.99958552340205 35.0 31.0 35.0 20.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 1.0 14 2.0 15 9.0 16 41.0 17 103.0 18 176.0 19 339.0 20 597.0 21 1005.0 22 1598.0 23 2584.0 24 3643.0 25 4975.0 26 6771.0 27 8870.0 28 11854.0 29 14253.0 30 17512.0 31 19472.0 32 22446.0 33 32086.0 34 71187.0 35 67245.0 36 73394.0 37 92877.0 38 109118.0 39 36185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 99.29672680476982 0.5877879818499361 0.08139117064569772 0.03409404273454278 8 99.11472478252513 0.568234045575713 0.16311659661232233 0.15392457528683284 9 95.80726838195356 2.2936600122003195 1.1839323467230445 0.7151392591230812 10 53.795719860615534 33.20642772982143 4.6774018333904355 8.32045057617261 11 38.46693797056882 27.529936742180517 16.996715941471894 17.00640934577877 12 38.507549992061435 14.989345611645454 22.892144164319916 23.610960231973195 13 24.980571409471125 14.16624188386299 24.66453300353475 36.18865370313114 14 22.425523736305976 23.544109167787813 28.63832738637408 25.392039709532128 15 31.25788633647812 17.498934561164546 26.048517159832542 25.1946619425248 16 31.979710702019737 18.10443807502361 26.644494397045182 23.27135682591147 17 37.61876509371684 18.884422866406506 19.983621489274583 23.51319055060208 18 37.94098722309035 17.926781371950966 20.509739364413505 23.622492040545172 19 31.396936549983707 20.07871712807828 25.191820772296918 23.33252554964109 20 31.45359282688081 27.323868336829086 20.226792235248894 20.995746601041205 21 30.205817713860732 19.086145952585884 28.6528674928344 22.055168840718984 22 28.511310364421867 18.595626269125674 24.073903851456933 28.81915951499553 23 27.72948716877387 24.04682917046186 23.03102725016504 25.192656410599234 24 28.04535844704978 27.055461314124795 20.25136000133702 24.647820237488403 25 28.452815683259658 21.306102666521824 19.612263827724806 30.62881782249371 26 31.270922293994268 21.422590645864844 20.57893021584537 26.727556844295513 27 26.519482907018528 19.75064553058854 22.052661925812032 31.6772096365809 28 26.391128863782598 26.474525566353858 19.85844287158746 27.275902698276077 29 34.037052202324745 21.544928093324085 19.53956329542321 24.87845640892796 30 29.789335583985828 24.213121192622985 21.372452347725808 24.625090875665375 31 34.04340305342235 19.501458188837546 22.73320575921918 23.72193299852092 32 31.802889637249415 20.581771386073253 25.029038431005525 22.58630054567181 33 37.426735411844334 19.015283824549382 20.945608302902173 22.61237246070411 34 28.995312069124 21.09886436754715 24.212118426660204 25.693705136668644 35 28.50763355589167 29.48934143345394 20.959981281702028 21.04304372895236 36 33.61405209369177 23.5725208700666 20.08974755366887 22.723679482572763 37 29.96097569128179 23.18194352756353 23.205842783009803 23.651237998144882 38 34.03304113847362 22.57460160943937 20.23297595868604 23.159381293400962 39 30.234563671460446 20.246011916202193 20.73352330177406 28.785901110563305 40 30.417568459667915 20.345620001838405 23.768895871111148 25.467915667382528 41 29.5695627104764 21.791274264847203 22.140738202876268 26.498424821800132 42 25.679165030208324 20.793856387201366 26.33497396986688 27.19200461272343 43 26.28349865044414 19.70318127501692 25.54913971036776 28.46418036417117 44 27.792828552089517 19.414551805396552 23.417593528816987 29.375026113696943 45 27.815557913912542 19.601567657455146 22.41115075750612 30.171723671126188 46 29.48583175258421 22.104137245234774 22.484519800449572 25.925511201731442 47 30.20347792661424 20.09359148985953 24.77567289774294 24.927257685783285 48 30.940678036918502 22.230318628884675 23.049244165155557 23.779759169041274 49 32.51970017297713 21.11791692083998 21.73194394538268 24.63043896080021 50 26.094644394120447 23.200661825535434 22.461790438626544 28.24290334171757 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 2.0 10 4.0 11 5.0 12 6.0 13 16.0 14 26.0 15 133.5 16 241.0 17 484.0 18 727.0 19 988.0 20 1249.0 21 1101.0 22 953.0 23 821.5 24 690.0 25 702.0 26 714.0 27 880.5 28 1047.0 29 1122.0 30 1197.0 31 1551.5 32 1906.0 33 2360.0 34 2814.0 35 3018.5 36 3223.0 37 3513.5 38 3804.0 39 5782.0 40 7760.0 41 7579.5 42 7399.0 43 7817.5 44 8236.0 45 10087.5 46 11939.0 47 12210.5 48 12482.0 49 13860.0 50 15238.0 51 16576.5 52 17915.0 53 20128.0 54 22341.0 55 24097.5 56 25854.0 57 28577.0 58 31300.0 59 35860.5 60 40421.0 61 47096.0 62 53771.0 63 62100.0 64 70429.0 65 68159.5 66 65890.0 67 60656.5 68 55423.0 69 49732.5 70 44042.0 71 36360.5 72 28679.0 73 25253.0 74 21827.0 75 18927.5 76 16028.0 77 12896.5 78 9765.0 79 8632.0 80 7499.0 81 5499.5 82 3500.0 83 2291.0 84 1082.0 85 879.0 86 676.0 87 407.5 88 139.0 89 103.5 90 68.0 91 53.0 92 38.0 93 19.5 94 1.0 95 1.0 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 598345.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.031445069316197 #Duplication Level Percentage of deduplicated Percentage of total 1 71.11949007989945 9.26789728333988 2 9.743116206892129 2.5393376730815835 3 3.7064111936183037 1.4489968162180682 4 1.7057186461980427 0.8891191536655274 5 1.006758749823657 0.6559760673190217 6 0.6348351352391212 0.4963691515764317 7 0.46298077539661164 0.4223315979911256 8 0.3667936336937145 0.38238808714036215 9 0.32318879612173446 0.37904553393109325 >10 6.862631936696036 25.292097368574986 >50 2.8638118323009247 25.555824816786306 >100 1.154245700434766 24.703640876083195 >500 0.03334487579033768 2.9927550159189096 >1k 0.015389942672463545 3.739815658190509 >5k 0.0012824952227052954 1.2344049001830049 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 7386 1.2344049001830049 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 4215 0.7044430888534207 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGA 3691 0.6168681947705754 No Hit GTCCCGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG 1917 0.32038372510842406 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGC 1864 0.3115259591038615 No Hit GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 1770 0.29581595902029767 No Hit GTCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC 1580 0.2640617035322431 No Hit GTCCCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC 1490 0.24902021409053304 No Hit GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 1424 0.23798978849994568 No Hit GTCCCGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA 1166 0.19487085210037688 No Hit GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 1103 0.18434180949117984 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG 1093 0.1826705328865454 Illumina Single End Adapter 2 (100% over 23bp) GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC 1064 0.17782383073310548 No Hit GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG 996 0.16645914982159124 No Hit GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 996 0.16645914982159124 No Hit GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 951 0.1589384051007362 No Hit GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 869 0.1452339369427337 No Hit GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA 818 0.13671042625909802 No Hit GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 789 0.1318637241056581 No Hit GTCCCGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA 786 0.13136234112426778 Illumina Single End Adapter 2 (100% over 34bp) GTCCCGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT 784 0.13102808580334088 No Hit GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 778 0.1300253198405602 No Hit GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC 752 0.12568000066851065 No Hit GTCCCGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG 671 0.11214266017097159 No Hit GTCCCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG 669 0.1118084048500447 No Hit GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 648 0.10829872398031236 No Hit GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGACACCTACTGCT 640 0.10696170269660481 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG 629 0.10512329843150692 No Hit GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGG 621 0.10378627714779935 No Hit GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC 620 0.1036191494873359 No Hit GTCCCGGGGACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCCTGTC 603 0.10077797925945733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.01571000008356383 0.0 0.0 0.0 0.0 8 0.0167127660463445 0.0 0.0 0.0 0.0 9 0.02506914906951675 0.0 0.0 0.0 0.0 10 0.0601659577668402 0.0 0.0 0.0 0.0 11 0.18467606481210672 0.0 0.0 0.0 0.0 12 0.2750921291228305 0.0 0.0 0.0 0.0 13 0.30500798034578713 0.0 0.0 0.0 0.0 14 0.49336085368808963 0.0 0.0 0.0 0.0 15 0.545337556092221 0.0 0.0 0.0 0.0 16 0.6228847905472595 0.0 0.0 0.0 0.0 17 0.6833850036350266 0.0 0.0 0.0 0.0 18 0.7117967059138123 0.0 0.0 0.0 0.0 19 0.7453893656669647 0.0 0.0 0.0 0.0 20 0.7849986211968012 0.0 0.0 0.0 0.0 21 0.8095663872849276 0.0 0.0 0.0 0.0 22 0.8291203235591507 0.0 0.0 0.0 0.0 23 0.8652198982192548 0.0 0.0 0.0 0.0 24 0.9108457495257752 0.0 0.0 0.0 0.0 25 0.9452740475812449 0.0 0.0 0.0 0.0 26 1.0600907503196317 0.0 0.0 0.0 0.0 27 1.1159113889144223 0.0 0.0 0.0 0.0 28 1.3443749007679515 0.0 0.0 0.0 0.0 29 1.3781346881815675 0.0 0.0 0.0 0.0 30 1.4844278802363184 0.0 0.0 0.0 0.0 31 1.5068229867384202 0.0 0.0 0.0 0.0 32 1.5720027743191638 0.0 0.0 0.0 0.0 33 1.6072667106769505 0.0 0.0 0.0 0.0 34 1.6431991576765912 0.0 0.0 0.0 0.0 35 1.8196859671259893 0.0 0.0 0.0 0.0 36 1.8499360736698727 0.0 0.0 0.0 0.0 37 1.90074288245076 0.0 0.0 0.0 0.0 38 1.9375109677527178 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGAA 20 7.853451E-4 44.000004 30 GAAATCG 20 7.853451E-4 44.000004 20 AGGTAAT 20 7.853451E-4 44.000004 16 CGAACTA 20 7.853451E-4 44.000004 30 AAATCGT 20 7.853451E-4 44.000004 21 CGAACGA 20 7.853451E-4 44.000004 29 CATATCG 20 7.853451E-4 44.000004 36 CGACGAA 20 7.853451E-4 44.000004 27 GCTATAA 20 7.853451E-4 44.000004 17 TAAACTA 20 7.853451E-4 44.000004 35 ATTCGAG 20 7.853451E-4 44.000004 23 CGCGAAT 20 7.853451E-4 44.000004 19 ATTCCTA 20 7.853451E-4 44.000004 41 ATATCTC 20 7.853451E-4 44.000004 32 GTTAATC 20 7.853451E-4 44.000004 36 AGTTGCG 20 7.853451E-4 44.000004 21 TGATTAG 20 7.853451E-4 44.000004 13 TCCGTTA 20 7.853451E-4 44.000004 44 GAACGAC 20 7.853451E-4 44.000004 9 AATTCGT 20 7.853451E-4 44.000004 13 >>END_MODULE