FastQCFastQC Report
Fri 17 Jun 2016
SRR1527737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884268
Sequences flagged as poor quality0
Sequence length52
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA109501.2383123668390126No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC61430.6946988921910552No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAG44460.5027887473028539No Hit
GTCCCGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG28400.32116960016646534No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC24950.28215427901948276No Hit
GTCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT24320.2750297421143816No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAG24080.2723156328172002No Hit
GTCCCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT21920.2478886491425676No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT20250.2290029719496804No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC16590.1876128051676641No Hit
GTCCCGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC16590.1876128051676641No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC15930.18014900460041525No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC15230.17223285248363618No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG15190.17178050093410596No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG14420.16307273360564897No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC14360.16239420628135362Illumina Single End Adapter 2 (100% over 25bp)
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC13810.15617437247531293No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG12980.1467880778225606No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT12040.1361578164086001No Hit
GTCCCGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA11940.13502693753477452No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC11070.12518829133249196No Hit
GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT11060.12507520344510942No Hit
GTCCCGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG10460.1182899302021559No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA10070.11387950259423613No Hit
GTCCCGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA10030.11342715104470591Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9740.11014760231061171No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC9270.10483247160363149No Hit
GTCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG9250.10460629582886637No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGACACCTACTGCTCT9150.10347541695504077No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGGAG8850.10008278033356403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAA253.4165125E-546.012
AACGTCC206.310661E-446.037
AGACTAC206.310661E-446.045
CCCCTAG206.310661E-446.035
ACACGTC206.310661E-446.020
AAATCGT206.310661E-446.021
CGAACAA453.092282E-1046.031
GTACATG253.4165125E-546.021
CGTATCG301.8609135E-646.026
CACGGGT206.310661E-446.030
GCGAATC301.8609135E-646.029
TCCGATA551.8189894E-1246.021
CCGATAG551.8189894E-1246.022
ACGCAAT206.310661E-446.024
CTATACT206.310661E-446.028
GGCAATA206.310661E-446.038
ATTACGA206.310661E-446.031
ATAGCTA301.8609135E-646.024
CCGTGTA253.4165125E-546.012
AAGACGT206.310661E-446.043
Produced by FastQC (version 0.11.5)