##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	884268
Sequences flagged as poor quality	0
Sequence length	52
%GC	61
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00071471544826	31.0	31.0	34.0	30.0	34.0
2	31.868090895520364	31.0	31.0	34.0	30.0	34.0
3	32.46683245350957	34.0	31.0	34.0	31.0	34.0
4	36.27350757915021	37.0	35.0	37.0	35.0	37.0
5	36.34180700873491	37.0	37.0	37.0	35.0	37.0
6	35.973431131738344	37.0	35.0	37.0	35.0	37.0
7	36.450339716013694	37.0	37.0	37.0	35.0	37.0
8	36.524229079871716	37.0	37.0	37.0	35.0	37.0
9	38.51705026078067	39.0	39.0	39.0	37.0	39.0
10	37.632558228953215	39.0	37.0	39.0	35.0	39.0
11	36.33696571627606	38.0	35.0	39.0	33.0	39.0
12	36.08091777605884	38.0	35.0	39.0	32.0	39.0
13	35.96211895036346	38.0	35.0	39.0	31.0	39.0
14	36.500530382191826	39.0	35.0	40.0	31.0	40.0
15	36.774912130711506	39.0	35.0	40.0	32.0	40.0
16	36.66178692432611	39.0	35.0	40.0	31.0	40.0
17	36.74680074366595	39.0	35.0	40.0	32.0	40.0
18	36.76734202753011	39.0	35.0	40.0	32.0	40.0
19	36.20597149280535	38.0	35.0	40.0	30.0	40.0
20	36.33659591888432	38.0	35.0	40.0	30.0	40.0
21	36.36363862539411	38.0	35.0	40.0	31.0	40.0
22	36.394637146204545	38.0	35.0	40.0	31.0	40.0
23	36.38604359764234	38.0	35.0	40.0	31.0	40.0
24	36.326213319943726	38.0	35.0	40.0	31.0	40.0
25	36.19698326751618	38.0	35.0	40.0	31.0	40.0
26	35.925740838750244	38.0	35.0	40.0	30.0	40.0
27	35.77968783219567	38.0	35.0	40.0	30.0	40.0
28	35.60314746208163	37.0	34.0	40.0	30.0	40.0
29	35.49482057475788	37.0	34.0	40.0	30.0	40.0
30	35.14812364577255	36.0	34.0	40.0	29.0	40.0
31	34.91331134904802	36.0	34.0	40.0	28.0	40.0
32	34.35041412784359	35.0	33.0	39.0	26.0	40.0
33	34.525959324548666	35.0	33.0	39.0	27.0	40.0
34	34.080199667973964	35.0	33.0	39.0	24.0	40.0
35	34.100346275111164	35.0	33.0	39.0	26.0	40.0
36	33.96512821904671	35.0	33.0	39.0	25.0	40.0
37	33.48074113277875	35.0	33.0	39.0	23.0	40.0
38	33.45536986524447	35.0	33.0	38.0	23.0	40.0
39	33.24532381585673	35.0	32.0	38.0	23.0	40.0
40	32.825601514472986	35.0	32.0	38.0	22.0	40.0
41	32.53688135271207	35.0	31.0	38.0	21.0	40.0
42	32.47262820773793	35.0	31.0	37.0	22.0	40.0
43	32.34595620332298	35.0	31.0	37.0	21.0	40.0
44	32.26717578833566	35.0	31.0	37.0	21.0	40.0
45	32.16813454744489	35.0	31.0	36.0	22.0	40.0
46	31.985714738065834	35.0	31.0	36.0	20.0	40.0
47	31.838619061189593	34.0	31.0	36.0	20.0	39.0
48	31.62322734736528	34.0	30.0	36.0	20.0	39.0
49	32.08274640719782	35.0	31.0	36.0	22.0	39.0
50	32.059998778650815	35.0	31.0	35.0	21.0	39.0
51	32.09105384340494	35.0	31.0	35.0	21.0	39.0
52	31.658868125952765	35.0	31.0	35.0	20.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	5.0
15	19.0
16	59.0
17	169.0
18	336.0
19	708.0
20	1278.0
21	2137.0
22	3783.0
23	5565.0
24	8037.0
25	11078.0
26	14006.0
27	17862.0
28	20652.0
29	24324.0
30	29184.0
31	35446.0
32	43553.0
33	60937.0
34	111614.0
35	97984.0
36	115265.0
37	125167.0
38	126399.0
39	28699.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.33764424360035	0.5630645912777574	0.06706111721785703	0.0322300479040291
8	99.14822203223457	0.5505118357782934	0.1526686479664536	0.14859748402068151
9	95.9269135601424	2.253728507647003	1.1459195628474625	0.6734383693631343
10	53.891919644270736	33.370765424056955	4.58232119674126	8.15499373493104
11	38.375809143834225	27.79236611525013	16.865362084797823	16.966462656117827
12	38.51140152080591	14.93348170464158	22.92936078202536	23.625755992527154
13	25.055299976930073	14.086792691808366	24.640041254461316	36.21786607680025
14	22.460611488824654	23.62654760773883	28.481976052508966	25.43086485092755
15	31.471567443354274	17.437586794953567	25.850307825229457	25.240537936462704
16	31.81693785142061	17.991717443128103	26.94545092664215	23.24589377880914
17	37.86058072891929	18.82008621820534	19.901432597357363	23.41790045551801
18	38.31033125703972	17.725395468342175	20.484739920476596	23.479533354141505
19	31.33122537511252	20.064618418850394	25.25772729534485	23.346428910692232
20	31.48106682589441	27.398820267158825	20.180081151867984	20.940031755078778
21	30.067015882062904	19.126215129349923	28.789575106189524	22.017193882397645
22	28.522122252529776	18.376329348116183	23.995553384268117	29.105995015085924
23	27.789425830178182	24.155007305477525	22.805416457454076	25.25015040689022
24	27.99501960943967	27.118249218562696	20.22237602174906	24.664355150248568
25	28.300922344809493	21.21166886057168	19.509922331238947	30.977486463379883
26	31.493958845056024	21.333803778944844	20.52239818697499	26.649839189024142
27	26.454310231739697	19.641782807927008	21.86723934372837	32.036667616604916
28	26.327651797871233	26.60686579181877	19.863548155084203	27.20193425522579
29	34.19562847462534	21.362528102340015	19.5847865126862	24.857056910348447
30	29.820823551231072	24.36749944586935	21.257469454961615	24.554207547937956
31	34.48988315759476	19.43064772218377	22.388687592449347	23.690781527772124
32	32.04922037210439	20.425255691713375	24.88996548557677	22.635558450605473
33	37.67941393333243	18.94923258559622	20.767572726820376	22.603780754250973
34	28.98159833896511	20.879982086878638	24.015796116109595	26.122623458046657
35	28.475982394477693	29.689528513979923	20.77447108795071	21.060018003591672
36	33.79348794709296	23.544785065161243	19.90098024580783	22.760746741937965
37	30.207244862417276	23.120592399589267	23.038151329687377	23.63401140830608
38	34.28609878453139	22.54226094351486	20.162213265661542	23.00942700629221
39	30.339444602767486	20.139482600297647	20.475919065260758	29.045153731674105
40	30.349848688406684	20.254492981765708	23.755920150904476	25.639738178923132
41	29.720853858784896	21.829241813567833	22.099521864412146	26.35038246323513
42	25.7495465175716	20.64837809351916	26.435650730321576	27.16642465858767
43	26.19149398146263	19.573025372398416	25.624471314126485	28.611009332012465
44	27.460679341557086	19.254909145191277	23.388836868460693	29.895574644790944
45	27.708907254361797	19.36686615370001	22.32897718791136	30.59524940402683
46	29.7246988469559	21.813748772996423	22.47056322291432	25.99098915713336
47	30.75164995227691	19.924954877932937	24.449035812672175	24.874359357117978
48	31.54247354874314	22.040376899311067	22.778614628144407	23.638534923801384
49	32.89918893367169	20.840966765731654	21.66198482812903	24.597859472467622
50	26.20777863724572	23.00999244572912	22.551081798730703	28.23114711829445
51	25.985900202201144	25.128015488517057	22.843866339164144	26.042217970117658
52	28.650363916821597	24.17468459788209	21.943008228274685	25.231943257021626
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.5
10	5.0
11	10.5
12	16.0
13	30.5
14	212.5
15	380.0
16	709.5
17	1039.0
18	1304.5
19	1570.0
20	1421.5
21	1273.0
22	1104.5
23	936.0
24	866.0
25	796.0
26	911.5
27	1027.0
28	1397.5
29	1768.0
30	2011.0
31	2254.0
32	2663.5
33	3073.0
34	3390.0
35	3707.0
36	4551.0
37	5395.0
38	6525.0
39	9066.5
40	10478.0
41	10683.0
42	10888.0
43	12500.0
44	14112.0
45	14888.5
46	15665.0
47	16165.5
48	16666.0
49	21345.0
50	26024.0
51	26144.0
52	26264.0
53	27884.0
54	29504.0
55	33729.0
56	37954.0
57	42442.5
58	46931.0
59	50497.0
60	54063.0
61	66985.5
62	79908.0
63	84639.0
64	91620.5
65	93871.0
66	88370.0
67	82869.0
68	74296.5
69	65724.0
70	57769.0
71	49814.0
72	42367.5
73	34921.0
74	30049.0
75	25177.0
76	21588.5
77	18000.0
78	15351.0
79	12702.0
80	10328.0
81	7954.0
82	5269.0
83	2584.0
84	1964.5
85	1345.0
86	868.0
87	391.0
88	252.5
89	71.5
90	29.0
91	16.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	884268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.360648341537894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.1335312679245	7.967623856851426
2	10.494001222925704	2.3843731517865505
3	4.564353217749631	1.555620354402615
4	2.2294263443350495	1.013109148054035
5	1.2236781259379164	0.6950888436006394
6	0.7737962425006244	0.5274496199431784
7	0.526861258485356	0.418983983770256
8	0.3898973257424286	0.3543589125652626
9	0.28692444226722696	0.29336829202708714
>10	4.283871899347964	13.124227493936191
>50	3.158168338399098	25.74156273571803
>100	1.862509532902032	35.751066249578166
>500	0.04798736316655799	3.6870363391630723
>1k	0.02299394485064237	4.544766630059154
>5k	9.997367326366247E-4	0.6977008973748191
>10k+	9.997367326366247E-4	1.243663491169505
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	10950	1.2383123668390126	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6143	0.6946988921910552	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAG	4446	0.5027887473028539	No Hit
GTCCCGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2840	0.32116960016646534	No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2495	0.28215427901948276	No Hit
GTCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	2432	0.2750297421143816	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAG	2408	0.2723156328172002	No Hit
GTCCCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	2192	0.2478886491425676	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2025	0.2290029719496804	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC	1659	0.1876128051676641	No Hit
GTCCCGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	1659	0.1876128051676641	No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1593	0.18014900460041525	No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1523	0.17223285248363618	No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1519	0.17178050093410596	No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1442	0.16307273360564897	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	1436	0.16239420628135362	Illumina Single End Adapter 2 (100% over 25bp)
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1381	0.15617437247531293	No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1298	0.1467880778225606	No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1204	0.1361578164086001	No Hit
GTCCCGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	1194	0.13502693753477452	No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1107	0.12518829133249196	No Hit
GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1106	0.12507520344510942	No Hit
GTCCCGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG	1046	0.1182899302021559	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	1007	0.11387950259423613	No Hit
GTCCCGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	1003	0.11342715104470591	Illumina Single End Adapter 2 (100% over 34bp)
GTCCCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	974	0.11014760231061171	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	927	0.10483247160363149	No Hit
GTCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	925	0.10460629582886637	No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGGACACCTACTGCTCT	915	0.10347541695504077	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGGAG	885	0.10008278033356403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.01277893127422908	0.0	0.0	0.0	0.0
8	0.01334437071114187	0.0	0.0	0.0	0.0
9	0.01990346817933025	0.0	0.0	0.0	0.0
10	0.05168116453382911	0.0	0.0	0.0	0.0
11	0.15719216346175593	0.0	0.0	0.0	0.0
12	0.23737147561598973	0.0	0.0	0.0	0.0
13	0.26191154717800486	0.0	0.0	0.0	0.0
14	0.4309779388149294	0.0	0.0	0.0	0.0
15	0.47474295123197946	0.0	0.0	0.0	0.0
16	0.5394292228148028	0.0	0.0	0.0	0.0
17	0.599026539465411	0.0	0.0	0.0	0.0
18	0.6248105777886342	0.0	0.0	0.0	0.0
19	0.6624688442870261	0.0	0.0	0.0	0.0
20	0.7012579896592436	0.0	0.0	0.0	0.0
21	0.7238755671357552	0.0	0.0	0.0	0.0
22	0.7440052110898506	0.0	0.0	0.0	0.0
23	0.7743127649083762	0.0	0.0	0.0	0.0
24	0.8161552832399227	0.0	0.0	0.0	0.0
25	0.8438618156486495	0.0	0.0	0.0	0.0
26	0.9512953086620798	0.0	0.0	0.0	0.0
27	0.9989053092501369	0.0	0.0	0.0	0.0
28	1.2016718913270636	0.0	0.0	0.0	0.0
29	1.2290391600736428	0.0	0.0	0.0	0.0
30	1.3173608001194208	0.0	0.0	0.0	0.0
31	1.3369250046366035	0.0	0.0	0.0	0.0
32	1.3893977843821104	0.0	0.0	0.0	0.0
33	1.4252466446823813	0.0	0.0	0.0	0.0
34	1.4599646261088268	0.0	0.0	0.0	0.0
35	1.625525293236892	0.0	0.0	0.0	0.0
36	1.6501784526862897	0.0	0.0	0.0	0.0
37	1.6932649377790443	0.0	0.0	0.0	0.0
38	1.7231201400480398	0.0	0.0	0.0	0.0
39	1.7412142020292491	0.0	0.0	0.0	0.0
40	1.7572726820375724	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAA	25	3.4165125E-5	46.0	12
AACGTCC	20	6.310661E-4	46.0	37
AGACTAC	20	6.310661E-4	46.0	45
CCCCTAG	20	6.310661E-4	46.0	35
ACACGTC	20	6.310661E-4	46.0	20
AAATCGT	20	6.310661E-4	46.0	21
CGAACAA	45	3.092282E-10	46.0	31
GTACATG	25	3.4165125E-5	46.0	21
CGTATCG	30	1.8609135E-6	46.0	26
CACGGGT	20	6.310661E-4	46.0	30
GCGAATC	30	1.8609135E-6	46.0	29
TCCGATA	55	1.8189894E-12	46.0	21
CCGATAG	55	1.8189894E-12	46.0	22
ACGCAAT	20	6.310661E-4	46.0	24
CTATACT	20	6.310661E-4	46.0	28
GGCAATA	20	6.310661E-4	46.0	38
ATTACGA	20	6.310661E-4	46.0	31
ATAGCTA	30	1.8609135E-6	46.0	24
CCGTGTA	25	3.4165125E-5	46.0	12
AAGACGT	20	6.310661E-4	46.0	43
>>END_MODULE