FastQCFastQC Report
Fri 17 Jun 2016
SRR1527736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892269
Sequences flagged as poor quality0
Sequence length52
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA106261.1908964673209537No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC59260.6641494885510983No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAG37780.4234149118707475No Hit
GTCCCGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG27200.30484080473489494No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC24230.27155487862965094No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGCAG22980.2575456504708782No Hit
GTCCCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT22150.24824352297345306No Hit
GTCCCGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT21080.2362516236695436No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT18660.20912975795415956No Hit
GTCCCGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC16050.17987848955864208No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC15870.1778611607037788No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC15870.1778611607037788No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGGAG14750.16530889227351842No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14460.16205875134068312No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC14250.15970520101000932No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC13810.1547739526981213Illumina Single End Adapter 2 (100% over 25bp)
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG13580.15219625471690712No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC12940.14502352989961548No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG12340.13829910038340457No Hit
GTCCCGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA11930.13370407354732708No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT10760.12059143599071581No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC10590.1186861809611227No Hit
GTCCCGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT10540.1181258118347718No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA10230.11465152325139615No Hit
GTCCCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9650.10815124138572561No Hit
GTCCCGGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG9610.10770294608464488No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC9160.10265962394748669No Hit
GTCCCGGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA9050.10142681186951469Illumina Single End Adapter 2 (100% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGT351.0192343E-746.00000425
ATACTGG351.0192343E-746.00000419
TAGTTCG351.0192343E-746.00000431
TATACCC351.0192343E-746.00000439
TCACGAT501.6370905E-1146.037
AGCGTAA405.6097633E-946.026
TGATAGC253.4165365E-546.040
CGAACTA301.8609317E-646.030
AAATCCG206.3106924E-446.032
ACCGGAT301.8609317E-646.032
GTACATG206.3106924E-446.021
GATAACG206.3106924E-446.031
TTTACGG253.4165365E-546.046
ACCATTA405.6097633E-946.018
CCGATAG600.046.022
GCAAATC206.3106924E-446.013
GTTAGCT301.8609317E-646.038
CACGATA501.6370905E-1146.038
TAACGTC206.3106924E-446.033
CGCGAAT301.8609317E-646.019
Produced by FastQC (version 0.11.5)