FastQCFastQC Report
Fri 17 Jun 2016
SRR1527735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760928
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32240.4236931746499012No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA26900.35351570713654906No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22320.2933260439883931No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA20410.26822511459691323No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAA18560.2439126960763699No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGA18160.23865595693679298No Hit
TATGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA18010.23668467975945162No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC17320.2276168047436814No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15470.20330438622313804No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC14770.19410509272887844No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA13870.1822774296648303No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA13520.17767778291770048No Hit
TATGTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA13460.17688927204676394Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT13440.1766264350897851No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC12630.1659815383321418No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG12530.16466735354724757No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA11530.15152550569830522No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA11180.1469258589511754No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11030.14495458177383405No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAA10880.14298330459649272No Hit
TATGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG9590.1260303208713571No Hit
TATGTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA9540.12537322847890997No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAA8750.1149911686782455No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC8690.11420265780730898No Hit
TATGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC8470.11131145128054165No Hit
TATGTAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA8410.11052294040960513Illumina Single End Adapter 2 (97% over 34bp)
TATGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT8220.10802598931830606No Hit
TATGTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8110.1065803860549224Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7950.10447769039909163No Hit
TATGTAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA7660.10066655452289835Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC7630.1002722990874301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC551.8189894E-1244.00000411
CACGGCA302.5265836E-644.00000434
ATTACCA302.5265836E-644.00000423
CATTAGC302.5265836E-644.00000417
GACATAT302.5265836E-644.0000049
TAATACT254.4414177E-544.024
GTACACC207.854803E-444.031
CATGCGA207.854803E-444.026
ACCGCGT254.4414177E-544.025
CGGTTAT207.854803E-444.040
CAACCGC254.4414177E-544.026
TTACTAC207.854803E-444.042
CAATTGG207.854803E-444.038
CGATTGG254.4414177E-544.030
CACGAAC207.854803E-444.030
CGTTCTA207.854803E-444.040
TACGGAT207.854803E-444.013
CGTTACA254.4414177E-544.042
AACGGTC254.4414177E-544.041
CTAGTAT207.854803E-444.032
Produced by FastQC (version 0.11.5)