##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527735_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	760928
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13895007149165	33.0	31.0	34.0	30.0	34.0
2	32.06970173262122	33.0	31.0	34.0	30.0	34.0
3	32.82640013246983	34.0	31.0	34.0	31.0	34.0
4	36.25602685142353	37.0	37.0	37.0	35.0	37.0
5	35.308782171243536	35.0	35.0	37.0	33.0	37.0
6	36.05128868960007	37.0	35.0	37.0	35.0	37.0
7	36.43306199798141	37.0	37.0	37.0	35.0	37.0
8	36.64266395769376	37.0	37.0	37.0	35.0	37.0
9	38.6857166512469	39.0	39.0	39.0	39.0	39.0
10	38.06298624837041	39.0	39.0	39.0	37.0	39.0
11	37.77776083939611	39.0	38.0	39.0	35.0	39.0
12	37.45221098448211	39.0	37.0	39.0	35.0	39.0
13	37.253440535766856	39.0	37.0	39.0	34.0	39.0
14	38.039645012405906	40.0	38.0	40.0	34.0	40.0
15	38.156029216956135	40.0	38.0	40.0	34.0	40.0
16	38.215730003364314	40.0	38.0	40.0	35.0	40.0
17	38.24131323857185	40.0	38.0	40.0	35.0	40.0
18	38.15823704739476	40.0	38.0	40.0	35.0	40.0
19	38.14892736237857	40.0	38.0	40.0	34.0	40.0
20	38.0949682492956	40.0	38.0	40.0	34.0	40.0
21	38.10646736616342	40.0	38.0	40.0	34.0	40.0
22	38.1439912843265	40.0	38.0	40.0	34.0	40.0
23	38.03660661718323	40.0	38.0	40.0	34.0	40.0
24	37.889835832036674	40.0	38.0	40.0	34.0	40.0
25	37.57335910887758	39.0	37.0	40.0	34.0	40.0
26	37.18392935993944	39.0	35.0	40.0	33.0	40.0
27	36.957994711720424	39.0	35.0	40.0	33.0	40.0
28	36.796102916438876	39.0	35.0	40.0	33.0	40.0
29	36.76031109382228	39.0	35.0	40.0	32.0	40.0
30	36.7444580827621	40.0	35.0	40.0	32.0	40.0
31	36.65185799444888	40.0	35.0	40.0	32.0	40.0
32	36.51927646242483	40.0	35.0	40.0	31.0	40.0
33	36.324522162412215	40.0	35.0	40.0	31.0	40.0
34	35.88925496236175	40.0	35.0	40.0	28.0	40.0
35	35.70296006980949	40.0	35.0	40.0	26.0	40.0
36	35.38096245636906	39.0	35.0	40.0	24.0	40.0
37	34.961766422053074	39.0	35.0	40.0	21.0	40.0
38	34.625479677446485	39.0	35.0	40.0	18.0	40.0
39	34.238843885361035	39.0	35.0	40.0	15.0	40.0
40	33.828673672147694	39.0	34.0	40.0	11.0	40.0
41	33.54889161655242	39.0	34.0	40.0	10.0	40.0
42	33.311620810378905	38.0	33.0	40.0	10.0	40.0
43	33.08201564405568	38.0	33.0	40.0	10.0	40.0
44	32.74773171706127	37.0	33.0	40.0	10.0	40.0
45	32.59969142941251	37.0	33.0	40.0	10.0	40.0
46	32.41162238740065	36.0	33.0	40.0	9.0	40.0
47	32.28128942554355	36.0	32.0	40.0	9.0	40.0
48	32.097070944951426	35.0	32.0	40.0	9.0	40.0
49	31.932414104882458	35.0	31.0	40.0	9.0	40.0
50	31.35477469616048	35.0	30.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	6.0
14	16.0
15	19.0
16	58.0
17	183.0
18	410.0
19	764.0
20	1383.0
21	1986.0
22	3064.0
23	4475.0
24	6303.0
25	8963.0
26	11835.0
27	16135.0
28	20954.0
29	24206.0
30	22194.0
31	18277.0
32	17592.0
33	20351.0
34	33467.0
35	43314.0
36	65532.0
37	113566.0
38	219916.0
39	105955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.38207031414274	0.3949125278607174	0.18700849489044954	0.036008663106102026
8	98.53994070398251	1.15792821397031	0.19357941881492075	0.10855166323226376
9	96.62714474956896	1.9145043946339206	0.7786544850498339	0.679696370747298
10	53.641080365027975	31.190204592287312	6.119895706295471	9.04881933638925
11	30.307860927709324	35.81035262206148	17.7609445308886	16.120841919340595
12	31.437402750325916	28.638714832415157	22.287785440935277	17.63609697632365
13	22.53642920223727	31.39600592960175	24.369191303250766	21.698373564910213
14	23.534946801799908	34.11860254846714	23.288011480718282	19.058439169014676
15	22.983383447579797	32.85409394844191	23.09167227385508	21.070850330123218
16	25.026415114176377	34.06774359729173	21.825718070566467	19.08012321796543
17	25.629625930442828	33.65942638462509	19.98073405105345	20.730213633878634
18	24.260508221540015	34.89725703351697	21.189915471634635	19.65231927330838
19	25.444588712729722	32.249043273476595	21.843722402119518	20.462645611674166
20	23.219279616468313	33.47031519407881	22.209197190798605	21.101207998654274
21	24.094001009293915	32.33328251818832	24.192302031204004	19.380414441313764
22	23.455438622313807	33.289351949198874	22.77745069178687	20.477758736700448
23	23.31311241010976	34.490911098027674	22.356648723663735	19.83932776819883
24	22.712135707977627	33.41196538962951	25.012221918499517	18.863676983893352
25	25.204355734051052	34.55491189705202	19.95957567601665	20.281156692880273
26	24.863324782371	33.229162286050716	22.4950323815131	19.412480550065183
27	25.09580407081879	33.08736174776063	21.45827200471004	20.358562176710542
28	27.47342718364944	33.322863661213674	20.237394339543293	18.96631481559359
29	28.405972707010385	32.81466840489507	19.9763972412633	18.802961646831235
30	27.558717776189074	32.68416985575508	20.319793725556163	19.437318642499683
31	27.99818116825771	33.713702216241224	18.75289120652677	19.535225408974306
32	27.651104966567143	34.0606469994533	20.301657975524623	17.98659005845494
33	27.905399722444173	35.043131544640225	18.692570124900122	18.358898608015476
34	24.950849489044955	35.954650111442874	20.298109676605407	18.796390722906768
35	26.105623659531517	37.53587724462761	19.26253206610875	17.09596702973212
36	26.61605302998444	36.3829429328399	18.68652487488961	18.31447916228605
37	25.715836452331892	35.76935005677278	18.905599478531478	19.60921401236385
38	26.42037091551369	34.70578031035788	19.901882963959796	18.971965810168637
39	26.585038269060934	33.83789267841373	18.63750578241305	20.939563270112284
40	25.689552756634004	34.043694015728164	20.04079229572312	20.225960931914717
41	25.716230707767355	33.46032738971361	20.490900584549394	20.33254131796964
42	24.932056646620968	32.54854598595399	20.926027166827872	21.593370200597164
43	25.700986164262584	32.03956221876446	21.98880840237184	20.27064321460112
44	26.550738046175198	32.19884141469363	20.6834286555364	20.566991883594767
45	25.855534294966148	32.73016632322638	21.05823415618823	20.356065225619243
46	25.709265528407414	33.75891017284158	20.253427393919004	20.278396904831993
47	25.210795239497035	33.75391627065898	20.949813911434457	20.08547457840952
48	25.292406114638965	33.38384183523277	20.80604209596703	20.517709954161234
49	24.821533706211362	33.94683859708146	20.328335926657974	20.903291770049204
50	23.70487089448673	34.064458135329495	20.733761932797847	21.49690903738593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	19.5
2	39.0
3	55.0
4	71.0
5	116.5
6	162.0
7	2318.5
8	4475.0
9	4379.0
10	4283.0
11	4249.0
12	4215.0
13	4136.5
14	4058.0
15	4593.5
16	5129.0
17	5347.0
18	5565.0
19	6830.5
20	8096.0
21	9356.5
22	10617.0
23	12880.0
24	15143.0
25	17367.0
26	19591.0
27	22203.0
28	24815.0
29	27294.0
30	29773.0
31	29344.0
32	28915.0
33	28110.0
34	27305.0
35	28474.0
36	29643.0
37	28284.0
38	26925.0
39	26782.0
40	26639.0
41	26046.0
42	25453.0
43	27838.5
44	30224.0
45	34535.5
46	38847.0
47	40450.5
48	42054.0
49	43395.0
50	44736.0
51	44846.5
52	44957.0
53	44373.5
54	43790.0
55	43123.5
56	42457.0
57	41661.5
58	40866.0
59	39344.5
60	37823.0
61	33419.5
62	29016.0
63	25815.0
64	22614.0
65	18185.5
66	13757.0
67	12522.0
68	11287.0
69	9315.5
70	7344.0
71	6840.0
72	6336.0
73	4395.5
74	2455.0
75	1628.5
76	802.0
77	535.0
78	268.0
79	252.0
80	236.0
81	152.5
82	69.0
83	67.5
84	66.0
85	37.5
86	9.0
87	5.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	760928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.780772107295633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88631757973836	19.3008308621995
2	8.88769247378977	4.404877635054217
3	3.3295172651809173	2.475240257272636
4	1.7100998862421217	1.6951038224671282
5	1.0618976802820344	1.3157322208167486
6	0.7127784406302841	1.059792006015157
7	0.4974502285718035	0.8629040524271989
8	0.40511528841971256	0.8031255711608192
9	0.2923022380289812	0.65191276323438
>10	3.6296064014977083	22.679419211717768
>50	1.1171335713633803	19.085307536274847
>100	0.43889311229297745	17.8998648392643
>500	0.020438649837172973	3.391053867477027
>1k	0.010757184124827878	4.374835354618288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3224	0.4236931746499012	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA	2690	0.35351570713654906	No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2232	0.2933260439883931	No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	2041	0.26822511459691323	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAA	1856	0.2439126960763699	No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGA	1816	0.23865595693679298	No Hit
TATGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA	1801	0.23668467975945162	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1732	0.2276168047436814	No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1547	0.20330438622313804	No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1477	0.19410509272887844	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA	1387	0.1822774296648303	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA	1352	0.17767778291770048	No Hit
TATGTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	1346	0.17688927204676394	Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1344	0.1766264350897851	No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1263	0.1659815383321418	No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1253	0.16466735354724757	No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1153	0.15152550569830522	No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA	1118	0.1469258589511754	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1103	0.14495458177383405	No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAA	1088	0.14298330459649272	No Hit
TATGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	959	0.1260303208713571	No Hit
TATGTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	954	0.12537322847890997	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAA	875	0.1149911686782455	No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	869	0.11420265780730898	No Hit
TATGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	847	0.11131145128054165	No Hit
TATGTAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	841	0.11052294040960513	Illumina Single End Adapter 2 (97% over 34bp)
TATGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	822	0.10802598931830606	No Hit
TATGTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	811	0.1065803860549224	Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	795	0.10447769039909163	No Hit
TATGTAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	766	0.10066655452289835	Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	763	0.1002722990874301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.1877970057613861	0.0	0.0	0.0	0.0
7	0.19607636990621977	0.0	0.0	0.0	0.0
8	0.2719048319946171	0.0	0.0	0.0	0.0
9	0.3970152235165482	0.0	0.0	0.0	0.0
10	0.6199009630346104	0.0	0.0	0.0	0.0
11	0.8710416754278986	0.0	0.0	0.0	0.0
12	1.0098195887127297	0.0	0.0	0.0	0.0
13	1.1190283443374407	0.0	0.0	0.0	0.0
14	1.2495268934774382	0.0	0.0	0.0	0.0
15	1.4063091383153203	0.0	0.0	0.0	0.0
16	1.5349678287564659	0.0	0.0	0.0	0.0
17	1.6491704865637749	0.0	0.0	0.0	0.0
18	1.7539110139198453	0.0	0.0	0.0	0.0
19	1.8633826065015349	0.0	0.0	0.0	0.0
20	1.9827105849699314	0.0	0.0	0.0	0.0
21	2.1007243786534335	0.0	0.0	0.0	0.0
22	2.2427877539005006	0.0	0.0	0.0	0.0
23	2.3973358846040624	0.0	0.0	0.0	0.0
24	2.556352243576265	0.0	0.0	0.0	0.0
25	2.7130030699356573	0.0	0.0	0.0	0.0
26	2.876750494133479	0.0	0.0	0.0	0.0
27	3.0341898313638085	0.0	0.0	0.0	0.0
28	3.197674418604651	0.0	0.0	0.0	0.0
29	3.3188422557718997	0.0	0.0	0.0	0.0
30	3.4367246309769124	0.0	0.0	0.0	0.0
31	3.5360770007149167	0.0	0.0	0.0	0.0
32	3.6322753269691743	0.0	0.0	0.0	0.0
33	3.7209827999495353	0.0	0.0	0.0	0.0
34	3.8032507674839144	0.0	0.0	0.0	0.0
35	3.9090426426679	0.0	0.0	0.0	0.0
36	3.9886822406324907	0.0	0.0	0.0	0.0
37	4.068978930989529	0.0	0.0	0.0	0.0
38	4.153086757222759	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTC	55	1.8189894E-12	44.000004	11
CACGGCA	30	2.5265836E-6	44.000004	34
ATTACCA	30	2.5265836E-6	44.000004	23
CATTAGC	30	2.5265836E-6	44.000004	17
GACATAT	30	2.5265836E-6	44.000004	9
TAATACT	25	4.4414177E-5	44.0	24
GTACACC	20	7.854803E-4	44.0	31
CATGCGA	20	7.854803E-4	44.0	26
ACCGCGT	25	4.4414177E-5	44.0	25
CGGTTAT	20	7.854803E-4	44.0	40
CAACCGC	25	4.4414177E-5	44.0	26
TTACTAC	20	7.854803E-4	44.0	42
CAATTGG	20	7.854803E-4	44.0	38
CGATTGG	25	4.4414177E-5	44.0	30
CACGAAC	20	7.854803E-4	44.0	30
CGTTCTA	20	7.854803E-4	44.0	40
TACGGAT	20	7.854803E-4	44.0	13
CGTTACA	25	4.4414177E-5	44.0	42
AACGGTC	25	4.4414177E-5	44.0	41
CTAGTAT	20	7.854803E-4	44.0	32
>>END_MODULE