FastQCFastQC Report
Fri 17 Jun 2016
SRR1527734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124555
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43340.38539689032550656No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA35340.31425763968858794No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT31750.2823339009652707No Hit
TATGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG29020.25805763168542223No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27940.24845383284943823No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA25550.2272009817216588No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAG24950.22186553792388988No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC23610.20994971344220603No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA22150.1969668002009684No Hit
TATGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT20590.18309464632676925No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20420.18158293725073474No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA18830.16744401118664717No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA18690.1661990743005011No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT18330.16299780802183975No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18060.16059685831284373No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC17940.15952976955328996No Hit
TATGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG15190.1350756521468492No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA15020.13356394307081468No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT14870.13223008212137247No Hit
TATGTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14790.13151868961500326Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA14770.13134084148841096No Hit
TATGTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC14750.1311629933618187No Hit
TATGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT13650.12138134639924236No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG13530.12031425763968859No Hit
TATGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC12980.11542343415840044No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA12710.11302248444940444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG206.3113944E-446.00000430
AGCGTAA206.3113944E-446.00000437
CTCGTCA206.3113944E-446.00000422
CAACGTC206.3113944E-446.00000429
TTTCGAA405.6115823E-946.00000415
CTATACA206.3113944E-446.00000416
GATTTCG206.3113944E-446.00000410
CGCGATT206.3113944E-446.00000428
GCGCATA206.3113944E-446.00000413
TCGATAG206.3113944E-446.00000423
GCACCGT206.3113944E-446.00000442
CTAACTG206.3113944E-446.00000446
CTAACGA206.3113944E-446.00000428
CTACGAA206.3113944E-446.00000420
ACGGACT206.3113944E-446.00000434
TCCGTTA206.3113944E-446.00000422
TCGTACG206.3113944E-446.00000425
ATGAATC206.3113944E-446.00000443
CTCGATA351.01954356E-746.00000424
TTAGTCT351.01954356E-746.00000429
Produced by FastQC (version 0.11.5)