##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527734_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1124555
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83980952465642	31.0	31.0	34.0	30.0	34.0
2	31.72583110652658	31.0	31.0	34.0	30.0	34.0
3	32.5306418983509	34.0	31.0	34.0	30.0	34.0
4	36.15340379083282	37.0	35.0	37.0	35.0	37.0
5	35.08775293338254	35.0	35.0	37.0	33.0	37.0
6	35.83205623557763	37.0	35.0	37.0	35.0	37.0
7	36.356878053985795	37.0	35.0	37.0	35.0	37.0
8	36.583570390065404	37.0	37.0	37.0	35.0	37.0
9	38.623597778676896	39.0	39.0	39.0	37.0	39.0
10	37.87220633939647	39.0	38.0	39.0	35.0	39.0
11	37.48755107575886	39.0	37.0	39.0	35.0	39.0
12	37.16872096073558	39.0	37.0	39.0	34.0	39.0
13	36.97649203462703	39.0	37.0	39.0	33.0	39.0
14	37.80145924387869	40.0	38.0	40.0	33.0	40.0
15	37.93320291137383	40.0	38.0	40.0	33.0	40.0
16	37.9898724384312	40.0	38.0	40.0	34.0	40.0
17	38.01202431183891	40.0	38.0	40.0	34.0	40.0
18	37.987033093090155	40.0	38.0	40.0	34.0	40.0
19	37.96861780882216	40.0	38.0	40.0	34.0	40.0
20	37.98322625394045	40.0	38.0	40.0	34.0	40.0
21	37.96886501771812	40.0	38.0	40.0	34.0	40.0
22	37.978289189946246	40.0	38.0	40.0	34.0	40.0
23	37.87826295734757	40.0	38.0	40.0	34.0	40.0
24	37.72005104241233	40.0	37.0	40.0	33.0	40.0
25	37.433206023716046	39.0	37.0	40.0	33.0	40.0
26	37.008307285993126	39.0	35.0	40.0	33.0	40.0
27	36.81509574898515	39.0	35.0	40.0	32.0	40.0
28	36.6727007571884	39.0	35.0	40.0	32.0	40.0
29	36.55741337684684	39.0	35.0	40.0	31.0	40.0
30	36.33340476899752	39.0	35.0	40.0	31.0	40.0
31	36.15111043924041	39.0	35.0	40.0	30.0	40.0
32	35.900560666219086	38.0	35.0	40.0	30.0	40.0
33	35.68112631218571	38.0	35.0	40.0	29.0	40.0
34	35.43471684355145	38.0	35.0	40.0	26.0	40.0
35	35.205757833098424	38.0	35.0	40.0	25.0	40.0
36	34.92713562253513	38.0	35.0	40.0	23.0	40.0
37	34.51521713033156	38.0	34.0	40.0	21.0	40.0
38	34.19290652747087	38.0	34.0	40.0	18.0	40.0
39	33.821243069480815	38.0	33.0	40.0	15.0	40.0
40	33.404118962611875	38.0	33.0	40.0	12.0	40.0
41	33.13639439600553	38.0	33.0	40.0	10.0	40.0
42	32.874626852399395	37.0	33.0	40.0	10.0	40.0
43	32.628388117966665	37.0	32.0	40.0	10.0	40.0
44	32.424935196588876	36.0	32.0	40.0	10.0	40.0
45	32.21437457483182	36.0	31.0	40.0	10.0	40.0
46	32.04106246470826	35.0	31.0	40.0	10.0	40.0
47	31.838780673244084	35.0	31.0	40.0	9.0	40.0
48	31.59073944804834	35.0	30.0	39.0	9.0	40.0
49	31.713077617368647	35.0	31.0	39.0	9.0	40.0
50	31.65139988706644	35.0	31.0	40.0	9.0	40.0
51	31.52798662582088	35.0	31.0	40.0	9.0	40.0
52	30.997146426808825	35.0	29.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	1.0
14	9.0
15	36.0
16	119.0
17	315.0
18	663.0
19	1226.0
20	2226.0
21	3616.0
22	5800.0
23	8905.0
24	12785.0
25	17221.0
26	23042.0
27	29193.0
28	34149.0
29	32256.0
30	27763.0
31	26612.0
32	31488.0
33	39601.0
34	60572.0
35	75758.0
36	114276.0
37	180364.0
38	285377.0
39	111177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.44013409748746	0.3845965737558412	0.1448572991094255	0.030412029647282703
8	98.57454726536274	1.127646046658456	0.18914148263090733	0.10866520534789316
9	96.6777080711926	1.9060872967529379	0.73300105375015	0.6832035783043071
10	53.41499526479363	31.328747815802693	6.200674933640418	9.055581985763258
11	30.176203031421316	35.35193921151033	18.022773452610146	16.449084304458207
12	31.51975670376282	27.897879605710703	22.70249120763324	17.879872482893234
13	22.664253860415897	30.462894211488102	24.742587067773474	22.130264860322526
14	23.736500215640856	33.328472151206476	23.489558091867448	19.44546954128522
15	23.2344349542708	31.994433353637664	23.355816300670043	21.415315391421498
16	25.328507720831794	32.95917051633757	22.310780708813706	19.40154105401692
17	25.949286606702206	32.577508436670506	20.323150046018203	21.150054910609086
18	24.71084117717675	34.02323585773928	21.39352899591394	19.872393969170027
19	25.617866622797465	31.206388304707193	22.20407183285833	20.971673239637013
20	23.488402079044597	32.6492701557505	22.52197535914206	21.34035240606284
21	24.499290830595214	31.311941167839723	24.56696204276358	19.62180595880148
22	23.720760656437434	32.504146084451186	22.781544699903517	20.993548559207863
23	23.72431761896928	33.61098390029834	22.565903846410357	20.09879463432202
24	22.96872985314191	32.66785528497939	25.13349725002334	19.229917611855356
25	25.36692291617573	33.66549435109888	20.1916313564032	20.77595137632219
26	25.32886341708498	32.30691251206033	22.579331379968075	19.78489269088662
27	25.384440956645072	32.249912187487496	21.538830915339847	20.826815940527588
28	27.74350743182859	32.52602140402204	20.392599739452493	19.337871424696882
29	28.63025819101778	31.963132083357415	20.1846952794661	19.221914446158703
30	27.640711214658243	32.06957418712291	20.561377611588583	19.728336986630268
31	28.315111310696228	32.845169867191906	19.00449511139962	19.83522371071224
32	27.68108273939469	33.23892561946726	20.801828278741368	18.278163362396683
33	28.06096633779584	34.344073878111786	18.935134342028626	18.65982544206375
34	24.83320068827225	35.301163571368235	20.595346603767712	19.270289136591806
35	26.19302746419695	36.96635558065191	19.4920657504524	17.348551204698747
36	26.93154180987146	35.70612375561889	18.876533384316463	18.48580105019319
37	25.885883749572052	35.11415626625643	19.231785017184574	19.768174966986944
38	26.297068618253444	34.348609005339895	20.154105401692224	19.200216974714444
39	26.5727332144715	33.288723094913095	18.789654574476124	21.348889116139272
40	25.61982295218998	33.7065772683417	20.254589593216874	20.41901018625145
41	25.750096704918835	33.01216925806208	20.7296219393449	20.508112097674193
42	24.8597000591345	32.20900711837127	21.089053003187928	21.842239819306304
43	25.69487486161193	31.56893171076559	22.27725633695106	20.458937090671423
44	26.65872278367888	31.600232981045835	20.890129873594443	20.850914361680843
45	26.067022066506308	32.081312163477996	21.348355571759495	20.5033101982562
46	25.72991094255061	33.3140664529525	20.478500384596572	20.477522219900315
47	25.465184006117976	33.194107891566	21.113596044657665	20.22711205765836
48	25.62435807941808	32.8431246137361	20.825482079578144	20.707035227267674
49	24.956271591874117	33.56696648896675	20.47716652364713	20.999595395512003
50	23.53828847855374	33.98971148587663	20.622290594946445	21.84970944062318
51	22.923823201177353	33.575769971233065	20.835797270920498	22.66460955666908
52	23.98646575756633	32.474356523246975	22.036538897608388	21.502638821578312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	21.5
2	43.0
3	78.0
4	113.0
5	157.5
6	202.0
7	3132.0
8	6062.0
9	5766.5
10	5471.0
11	5526.0
12	5581.0
13	5394.0
14	5368.0
15	5529.0
16	5810.0
17	6091.0
18	7116.5
19	8142.0
20	9430.5
21	10719.0
22	13109.0
23	15499.0
24	19349.0
25	23199.0
26	27168.5
27	31138.0
28	36116.5
29	41095.0
30	40841.5
31	40588.0
32	41263.0
33	41938.0
34	42089.5
35	42241.0
36	41362.5
37	40484.0
38	39163.5
39	37733.0
40	37623.0
41	38965.5
42	40308.0
43	42840.0
44	45372.0
45	47457.5
46	49543.0
47	54300.0
48	59057.0
49	62140.0
50	65223.0
51	63026.0
52	60829.0
53	61919.0
54	63009.0
55	65202.5
56	67396.0
57	61916.0
58	56436.0
59	58134.5
60	59833.0
61	51167.5
62	42502.0
63	39176.0
64	30015.0
65	24180.0
66	21301.5
67	18423.0
68	15868.0
69	13313.0
70	11940.5
71	10568.0
72	7595.0
73	4622.0
74	3246.0
75	1870.0
76	1412.5
77	955.0
78	631.0
79	307.0
80	178.5
81	50.0
82	72.0
83	94.0
84	49.5
85	5.0
86	3.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1124555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.241192788041626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.90408623747986	19.369703590350532
2	8.1268411748583	3.9400864735506946
3	3.228955390156275	2.3482119035029325
4	1.6964367376952	1.6449460004474301
5	1.003394633248832	1.216174137353563
6	0.6655268659227596	0.9679899037472847
7	0.4975280164920149	0.8442470795664421
8	0.3428164272896868	0.6648223283869561
9	0.27447494542585193	0.5988240060799753
>10	2.484025573817522	13.999134038365677
>50	1.0820429575800472	18.62828434089071
>100	0.6605153798946426	26.88176364888861
>500	0.022359288664580017	3.7237375873787304
>1k	0.010996371474383615	5.172074961490434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4334	0.38539689032550656	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA	3534	0.31425763968858794	No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	3175	0.2823339009652707	No Hit
TATGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	2902	0.25805763168542223	No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2794	0.24845383284943823	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA	2555	0.2272009817216588	No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAG	2495	0.22186553792388988	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2361	0.20994971344220603	No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2215	0.1969668002009684	No Hit
TATGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2059	0.18309464632676925	No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2042	0.18158293725073474	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA	1883	0.16744401118664717	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA	1869	0.1661990743005011	No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1833	0.16299780802183975	No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1806	0.16059685831284373	No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1794	0.15952976955328996	No Hit
TATGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1519	0.1350756521468492	No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA	1502	0.13356394307081468	No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1487	0.13223008212137247	No Hit
TATGTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1479	0.13151868961500326	Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA	1477	0.13134084148841096	No Hit
TATGTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	1475	0.1311629933618187	No Hit
TATGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1365	0.12138134639924236	No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1353	0.12031425763968859	No Hit
TATGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1298	0.11542343415840044	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA	1271	0.11302248444940444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.16175287113569367	0.0	0.0	0.0	0.0
7	0.16904464432597782	0.0	0.0	0.0	0.0
8	0.2340481345954622	0.0	0.0	0.0	0.0
9	0.34804878374112425	0.0	0.0	0.0	0.0
10	0.5473276095877925	0.0	0.0	0.0	0.0
11	0.7835099217023622	0.0	0.0	0.0	0.0
12	0.9114716487855196	0.0	0.0	0.0	0.0
13	1.0123115365633517	0.0	0.0	0.0	0.0
14	1.1373387695577362	0.0	0.0	0.0	0.0
15	1.2845080943128615	0.0	0.0	0.0	0.0
16	1.402954946623331	0.0	0.0	0.0	0.0
17	1.5086856578824512	0.0	0.0	0.0	0.0
18	1.6091698494070987	0.0	0.0	0.0	0.0
19	1.7106322056280039	0.0	0.0	0.0	0.0
20	1.824010386330593	0.0	0.0	0.0	0.0
21	1.9328534398050785	0.0	0.0	0.0	0.0
22	2.0576139006095744	0.0	0.0	0.0	0.0
23	2.197758224364304	0.0	0.0	0.0	0.0
24	2.3331006487010417	0.0	0.0	0.0	0.0
25	2.4691544655441486	0.0	0.0	0.0	0.0
26	2.6040522695644053	0.0	0.0	0.0	0.0
27	2.730324439444936	0.0	0.0	0.0	0.0
28	2.8572190777685393	0.0	0.0	0.0	0.0
29	2.9465877613811684	0.0	0.0	0.0	0.0
30	3.0372013818799437	0.0	0.0	0.0	0.0
31	3.115988101960331	0.0	0.0	0.0	0.0
32	3.2002881139650796	0.0	0.0	0.0	0.0
33	3.2777409730960247	0.0	0.0	0.0	0.0
34	3.3561719969232273	0.0	0.0	0.0	0.0
35	3.4594128344100556	0.0	0.0	0.0	0.0
36	3.528062211274682	0.0	0.0	0.0	0.0
37	3.595377727189866	0.0	0.0	0.0	0.0
38	3.669095775662373	0.0	0.0	0.0	0.0
39	3.746192938540134	0.0	0.0	0.0	0.0
40	3.8298704821018092	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	6.3113944E-4	46.000004	30
AGCGTAA	20	6.3113944E-4	46.000004	37
CTCGTCA	20	6.3113944E-4	46.000004	22
CAACGTC	20	6.3113944E-4	46.000004	29
TTTCGAA	40	5.6115823E-9	46.000004	15
CTATACA	20	6.3113944E-4	46.000004	16
GATTTCG	20	6.3113944E-4	46.000004	10
CGCGATT	20	6.3113944E-4	46.000004	28
GCGCATA	20	6.3113944E-4	46.000004	13
TCGATAG	20	6.3113944E-4	46.000004	23
GCACCGT	20	6.3113944E-4	46.000004	42
CTAACTG	20	6.3113944E-4	46.000004	46
CTAACGA	20	6.3113944E-4	46.000004	28
CTACGAA	20	6.3113944E-4	46.000004	20
ACGGACT	20	6.3113944E-4	46.000004	34
TCCGTTA	20	6.3113944E-4	46.000004	22
TCGTACG	20	6.3113944E-4	46.000004	25
ATGAATC	20	6.3113944E-4	46.000004	43
CTCGATA	35	1.01954356E-7	46.000004	24
TTAGTCT	35	1.01954356E-7	46.000004	29
>>END_MODULE