FastQCFastQC Report
Fri 17 Jun 2016
SRR1527733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135558
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39770.3502242950161947No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA33250.292807588868204No Hit
TATGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT31240.2751070398869983No Hit
TATGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG27820.2449896878891259No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27600.2430523143687949No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA24290.213903649131088No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAG23190.2042167815294331No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC22120.19479410122600518No Hit
TATGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA21290.1874849193083929No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19860.17489199142624154No Hit
TATGTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT19550.17216205601122972No Hit
TATGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT18680.16450062436264815No Hit
TATGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18080.15921687839810914No Hit
TATGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC17940.15798400433971668No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA17730.15613469325212803No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA17110.15067482242210437No Hit
TATGTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14550.12813083964007121Illumina Single End Adapter 2 (100% over 34bp)
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA14530.1279547147745866No Hit
TATGTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC14160.12469640476312085No Hit
TATGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG13810.12161421961713977No Hit
TATGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT13750.12108584502068588No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA13510.11897234663487026No Hit
TATGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT13390.11791559744196245No Hit
TATGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG13210.11633047365260075No Hit
TATGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC12020.10585104415626503No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA12000.10567491929078039No Hit
TATGTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA11600.10215242198108772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAA253.417129E-546.025
GGTACGT206.3114235E-446.08
CGAACGA206.3114235E-446.027
CATGCTA206.3114235E-446.045
TAAACGT206.3114235E-446.043
GTTAGCT206.3114235E-446.010
TTCGTAC253.417129E-546.017
CCGCTAT301.8613828E-646.015
ATCCGTA206.3114235E-446.017
GTAATCC301.8613828E-646.027
CGTTACA301.8613828E-646.042
TACGCTT600.046.011
TAACCCG301.8613828E-646.018
TAATTCG206.3114235E-446.035
CATAGCG206.3114235E-446.038
CCATAGA301.8613828E-646.028
TTGCGAT253.417129E-546.040
CTCGATA301.8613828E-646.024
TTACGGC206.3114235E-446.044
TTACGCT301.8613828E-646.045
Produced by FastQC (version 0.11.5)